Determination of ornithine, putrescine, N-methylputrescine and N-methylpyrroline pools in tobacco tissue by high-performance liquid chromatography
A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and...
Ausführliche Beschreibung
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| Autor*in: |
Feth, Friedhelm [verfasserIn] Wagner, Karl G. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1989 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Physiologia plantarum - Oxford [u.a.] : Wiley-Blackwell, 1948, 75(1989), 1, Seite 0 |
| Übergeordnetes Werk: |
volume:75 ; year:1989 ; number:1 ; pages:0 |
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| DOI / URN: |
10.1111/j.1399-3054.1989.tb02065.x |
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| 520 | |a A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and N-methylputrescine with dansyl chloride and the products were separated by reversed-phase high-performance liquid chromatography (HPLC). N-methylpyrroline was determined by cation-exchange HPLC without derivatization. The metabolites in the roots of tobacco (Nicotiana) species with different nicotine-producing capacities were determined. Furthermore, the specific activities of the enzymes ornithine decarboxylase (EC 4.1.1.17), putrescine N-methyltransferase (EC 2.1.1.53) and N-methylputrescine oxidase were determined. Both the metabolite pools and the enzyme activities were correlated with the different nicotine-producing capacities of the different tobacco species. | ||
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10.1111/j.1399-3054.1989.tb02065.x doi (DE-627)NLEJ240960300 DE-627 ger DE-627 rakwb Feth, Friedhelm verfasserin aut Determination of ornithine, putrescine, N-methylputrescine and N-methylpyrroline pools in tobacco tissue by high-performance liquid chromatography Oxford, UK Blackwell Publishing Ltd 1989 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and N-methylputrescine with dansyl chloride and the products were separated by reversed-phase high-performance liquid chromatography (HPLC). N-methylpyrroline was determined by cation-exchange HPLC without derivatization. The metabolites in the roots of tobacco (Nicotiana) species with different nicotine-producing capacities were determined. Furthermore, the specific activities of the enzymes ornithine decarboxylase (EC 4.1.1.17), putrescine N-methyltransferase (EC 2.1.1.53) and N-methylputrescine oxidase were determined. Both the metabolite pools and the enzyme activities were correlated with the different nicotine-producing capacities of the different tobacco species. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Callus culture Wagner, Karl G. verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 75(1989), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:75 year:1989 number:1 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1989.tb02065.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 75 1989 1 0 |
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10.1111/j.1399-3054.1989.tb02065.x doi (DE-627)NLEJ240960300 DE-627 ger DE-627 rakwb Feth, Friedhelm verfasserin aut Determination of ornithine, putrescine, N-methylputrescine and N-methylpyrroline pools in tobacco tissue by high-performance liquid chromatography Oxford, UK Blackwell Publishing Ltd 1989 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and N-methylputrescine with dansyl chloride and the products were separated by reversed-phase high-performance liquid chromatography (HPLC). N-methylpyrroline was determined by cation-exchange HPLC without derivatization. The metabolites in the roots of tobacco (Nicotiana) species with different nicotine-producing capacities were determined. Furthermore, the specific activities of the enzymes ornithine decarboxylase (EC 4.1.1.17), putrescine N-methyltransferase (EC 2.1.1.53) and N-methylputrescine oxidase were determined. Both the metabolite pools and the enzyme activities were correlated with the different nicotine-producing capacities of the different tobacco species. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Callus culture Wagner, Karl G. verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 75(1989), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:75 year:1989 number:1 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1989.tb02065.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 75 1989 1 0 |
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10.1111/j.1399-3054.1989.tb02065.x doi (DE-627)NLEJ240960300 DE-627 ger DE-627 rakwb Feth, Friedhelm verfasserin aut Determination of ornithine, putrescine, N-methylputrescine and N-methylpyrroline pools in tobacco tissue by high-performance liquid chromatography Oxford, UK Blackwell Publishing Ltd 1989 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and N-methylputrescine with dansyl chloride and the products were separated by reversed-phase high-performance liquid chromatography (HPLC). N-methylpyrroline was determined by cation-exchange HPLC without derivatization. The metabolites in the roots of tobacco (Nicotiana) species with different nicotine-producing capacities were determined. Furthermore, the specific activities of the enzymes ornithine decarboxylase (EC 4.1.1.17), putrescine N-methyltransferase (EC 2.1.1.53) and N-methylputrescine oxidase were determined. Both the metabolite pools and the enzyme activities were correlated with the different nicotine-producing capacities of the different tobacco species. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Callus culture Wagner, Karl G. verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 75(1989), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:75 year:1989 number:1 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1989.tb02065.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 75 1989 1 0 |
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10.1111/j.1399-3054.1989.tb02065.x doi (DE-627)NLEJ240960300 DE-627 ger DE-627 rakwb Feth, Friedhelm verfasserin aut Determination of ornithine, putrescine, N-methylputrescine and N-methylpyrroline pools in tobacco tissue by high-performance liquid chromatography Oxford, UK Blackwell Publishing Ltd 1989 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and N-methylputrescine with dansyl chloride and the products were separated by reversed-phase high-performance liquid chromatography (HPLC). N-methylpyrroline was determined by cation-exchange HPLC without derivatization. The metabolites in the roots of tobacco (Nicotiana) species with different nicotine-producing capacities were determined. Furthermore, the specific activities of the enzymes ornithine decarboxylase (EC 4.1.1.17), putrescine N-methyltransferase (EC 2.1.1.53) and N-methylputrescine oxidase were determined. Both the metabolite pools and the enzyme activities were correlated with the different nicotine-producing capacities of the different tobacco species. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Callus culture Wagner, Karl G. verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 75(1989), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:75 year:1989 number:1 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1989.tb02065.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 75 1989 1 0 |
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10.1111/j.1399-3054.1989.tb02065.x doi (DE-627)NLEJ240960300 DE-627 ger DE-627 rakwb Feth, Friedhelm verfasserin aut Determination of ornithine, putrescine, N-methylputrescine and N-methylpyrroline pools in tobacco tissue by high-performance liquid chromatography Oxford, UK Blackwell Publishing Ltd 1989 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and N-methylputrescine with dansyl chloride and the products were separated by reversed-phase high-performance liquid chromatography (HPLC). N-methylpyrroline was determined by cation-exchange HPLC without derivatization. The metabolites in the roots of tobacco (Nicotiana) species with different nicotine-producing capacities were determined. Furthermore, the specific activities of the enzymes ornithine decarboxylase (EC 4.1.1.17), putrescine N-methyltransferase (EC 2.1.1.53) and N-methylputrescine oxidase were determined. Both the metabolite pools and the enzyme activities were correlated with the different nicotine-producing capacities of the different tobacco species. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Callus culture Wagner, Karl G. verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 75(1989), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:75 year:1989 number:1 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1989.tb02065.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 75 1989 1 0 |
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Determination of ornithine, putrescine, N-methylputrescine and N-methylpyrroline pools in tobacco tissue by high-performance liquid chromatography |
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A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and N-methylputrescine with dansyl chloride and the products were separated by reversed-phase high-performance liquid chromatography (HPLC). N-methylpyrroline was determined by cation-exchange HPLC without derivatization. The metabolites in the roots of tobacco (Nicotiana) species with different nicotine-producing capacities were determined. Furthermore, the specific activities of the enzymes ornithine decarboxylase (EC 4.1.1.17), putrescine N-methyltransferase (EC 2.1.1.53) and N-methylputrescine oxidase were determined. Both the metabolite pools and the enzyme activities were correlated with the different nicotine-producing capacities of the different tobacco species. |
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A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and N-methylputrescine with dansyl chloride and the products were separated by reversed-phase high-performance liquid chromatography (HPLC). N-methylpyrroline was determined by cation-exchange HPLC without derivatization. The metabolites in the roots of tobacco (Nicotiana) species with different nicotine-producing capacities were determined. Furthermore, the specific activities of the enzymes ornithine decarboxylase (EC 4.1.1.17), putrescine N-methyltransferase (EC 2.1.1.53) and N-methylputrescine oxidase were determined. Both the metabolite pools and the enzyme activities were correlated with the different nicotine-producing capacities of the different tobacco species. |
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A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and N-methylputrescine with dansyl chloride and the products were separated by reversed-phase high-performance liquid chromatography (HPLC). N-methylpyrroline was determined by cation-exchange HPLC without derivatization. The metabolites in the roots of tobacco (Nicotiana) species with different nicotine-producing capacities were determined. Furthermore, the specific activities of the enzymes ornithine decarboxylase (EC 4.1.1.17), putrescine N-methyltransferase (EC 2.1.1.53) and N-methylputrescine oxidase were determined. Both the metabolite pools and the enzyme activities were correlated with the different nicotine-producing capacities of the different tobacco species. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ240960300</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707123235.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1989 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1399-3054.1989.tb02065.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ240960300</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Feth, Friedhelm</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Determination of ornithine, putrescine, N-methylputrescine and N-methylpyrroline pools in tobacco tissue by high-performance liquid chromatography</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1989</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A procedure for the determination of metabolites of the biochemical pathway ornithine to N-methyl-δ1-pyrrolinium salt (N-methylpyrroline) is described. Plant tissue was extracted with 0.5 M HCl and the extract purified on C18-cartridges. Ornithine was reacted with o-phthaldialdehyde, putrescine and N-methylputrescine with dansyl chloride and the products were separated by reversed-phase high-performance liquid chromatography (HPLC). N-methylpyrroline was determined by cation-exchange HPLC without derivatization. The metabolites in the roots of tobacco (Nicotiana) species with different nicotine-producing capacities were determined. Furthermore, the specific activities of the enzymes ornithine decarboxylase (EC 4.1.1.17), putrescine N-methyltransferase (EC 2.1.1.53) and N-methylputrescine oxidase were determined. Both the metabolite pools and the enzyme activities were correlated with the different nicotine-producing capacities of the different tobacco species.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Callus culture</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wagner, Karl G.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Physiologia plantarum</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1948</subfield><subfield code="g">75(1989), 1, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927738</subfield><subfield code="w">(DE-600)2020837-6</subfield><subfield code="x">1399-3054</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:75</subfield><subfield code="g">year:1989</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1399-3054.1989.tb02065.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">75</subfield><subfield code="j">1989</subfield><subfield code="e">1</subfield><subfield code="h">0</subfield></datafield></record></collection>
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