The use of colloid gold labelling in the detection of plasma membrane from symbiotic and non-symbiotic Glycine max root cells
Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ostrowski, Elke D. [verfasserIn] Mellor, Robert B. [verfasserIn] Werner, Dietrich [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1986 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Physiologia plantarum - Oxford [u.a.] : Wiley-Blackwell, 1948, 66(1986), 2, Seite 0 |
| Übergeordnetes Werk: |
volume:66 ; year:1986 ; number:2 ; pages:0 |
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| DOI / URN: |
10.1111/j.1399-3054.1986.tb02419.x |
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| 520 | |a Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase I activity. After sucrose density gradient centrifugation one of the several GS II activity peaks co-sedimented with the single radioactive particulate peak from [125I]-labelled protoplasts at 1.14 g ml−1. This peak is presumed to be the plasma membrane peak because labelling of protoplasts with colloidal gold prior to disruption moved the 125I peak and the corresponding glucan synthetase II activity into denser regions of the gradient, leaving endoplasmic reticulum-contaminating IDPase (EC 3.1.3.31) and other glucan synthetase II peaks unmoved. Results are discussed in relation to various strategies of plasma membrane isolation. | ||
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10.1111/j.1399-3054.1986.tb02419.x doi (DE-627)NLEJ240970659 DE-627 ger DE-627 rakwb Ostrowski, Elke D. verfasserin aut The use of colloid gold labelling in the detection of plasma membrane from symbiotic and non-symbiotic Glycine max root cells Oxford, UK Blackwell Publishing Ltd 1986 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase I activity. After sucrose density gradient centrifugation one of the several GS II activity peaks co-sedimented with the single radioactive particulate peak from [125I]-labelled protoplasts at 1.14 g ml−1. This peak is presumed to be the plasma membrane peak because labelling of protoplasts with colloidal gold prior to disruption moved the 125I peak and the corresponding glucan synthetase II activity into denser regions of the gradient, leaving endoplasmic reticulum-contaminating IDPase (EC 3.1.3.31) and other glucan synthetase II peaks unmoved. Results are discussed in relation to various strategies of plasma membrane isolation. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Glucan synthetase Mellor, Robert B. verfasserin aut Werner, Dietrich verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 66(1986), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:66 year:1986 number:2 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1986.tb02419.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 66 1986 2 0 |
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10.1111/j.1399-3054.1986.tb02419.x doi (DE-627)NLEJ240970659 DE-627 ger DE-627 rakwb Ostrowski, Elke D. verfasserin aut The use of colloid gold labelling in the detection of plasma membrane from symbiotic and non-symbiotic Glycine max root cells Oxford, UK Blackwell Publishing Ltd 1986 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase I activity. After sucrose density gradient centrifugation one of the several GS II activity peaks co-sedimented with the single radioactive particulate peak from [125I]-labelled protoplasts at 1.14 g ml−1. This peak is presumed to be the plasma membrane peak because labelling of protoplasts with colloidal gold prior to disruption moved the 125I peak and the corresponding glucan synthetase II activity into denser regions of the gradient, leaving endoplasmic reticulum-contaminating IDPase (EC 3.1.3.31) and other glucan synthetase II peaks unmoved. Results are discussed in relation to various strategies of plasma membrane isolation. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Glucan synthetase Mellor, Robert B. verfasserin aut Werner, Dietrich verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 66(1986), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:66 year:1986 number:2 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1986.tb02419.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 66 1986 2 0 |
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10.1111/j.1399-3054.1986.tb02419.x doi (DE-627)NLEJ240970659 DE-627 ger DE-627 rakwb Ostrowski, Elke D. verfasserin aut The use of colloid gold labelling in the detection of plasma membrane from symbiotic and non-symbiotic Glycine max root cells Oxford, UK Blackwell Publishing Ltd 1986 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase I activity. After sucrose density gradient centrifugation one of the several GS II activity peaks co-sedimented with the single radioactive particulate peak from [125I]-labelled protoplasts at 1.14 g ml−1. This peak is presumed to be the plasma membrane peak because labelling of protoplasts with colloidal gold prior to disruption moved the 125I peak and the corresponding glucan synthetase II activity into denser regions of the gradient, leaving endoplasmic reticulum-contaminating IDPase (EC 3.1.3.31) and other glucan synthetase II peaks unmoved. Results are discussed in relation to various strategies of plasma membrane isolation. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Glucan synthetase Mellor, Robert B. verfasserin aut Werner, Dietrich verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 66(1986), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:66 year:1986 number:2 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1986.tb02419.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 66 1986 2 0 |
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10.1111/j.1399-3054.1986.tb02419.x doi (DE-627)NLEJ240970659 DE-627 ger DE-627 rakwb Ostrowski, Elke D. verfasserin aut The use of colloid gold labelling in the detection of plasma membrane from symbiotic and non-symbiotic Glycine max root cells Oxford, UK Blackwell Publishing Ltd 1986 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase I activity. After sucrose density gradient centrifugation one of the several GS II activity peaks co-sedimented with the single radioactive particulate peak from [125I]-labelled protoplasts at 1.14 g ml−1. This peak is presumed to be the plasma membrane peak because labelling of protoplasts with colloidal gold prior to disruption moved the 125I peak and the corresponding glucan synthetase II activity into denser regions of the gradient, leaving endoplasmic reticulum-contaminating IDPase (EC 3.1.3.31) and other glucan synthetase II peaks unmoved. Results are discussed in relation to various strategies of plasma membrane isolation. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Glucan synthetase Mellor, Robert B. verfasserin aut Werner, Dietrich verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 66(1986), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:66 year:1986 number:2 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1986.tb02419.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 66 1986 2 0 |
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10.1111/j.1399-3054.1986.tb02419.x doi (DE-627)NLEJ240970659 DE-627 ger DE-627 rakwb Ostrowski, Elke D. verfasserin aut The use of colloid gold labelling in the detection of plasma membrane from symbiotic and non-symbiotic Glycine max root cells Oxford, UK Blackwell Publishing Ltd 1986 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase I activity. After sucrose density gradient centrifugation one of the several GS II activity peaks co-sedimented with the single radioactive particulate peak from [125I]-labelled protoplasts at 1.14 g ml−1. This peak is presumed to be the plasma membrane peak because labelling of protoplasts with colloidal gold prior to disruption moved the 125I peak and the corresponding glucan synthetase II activity into denser regions of the gradient, leaving endoplasmic reticulum-contaminating IDPase (EC 3.1.3.31) and other glucan synthetase II peaks unmoved. Results are discussed in relation to various strategies of plasma membrane isolation. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Glucan synthetase Mellor, Robert B. verfasserin aut Werner, Dietrich verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 66(1986), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:66 year:1986 number:2 pages:0 http://dx.doi.org/10.1111/j.1399-3054.1986.tb02419.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 66 1986 2 0 |
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| abstract |
Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase I activity. After sucrose density gradient centrifugation one of the several GS II activity peaks co-sedimented with the single radioactive particulate peak from [125I]-labelled protoplasts at 1.14 g ml−1. This peak is presumed to be the plasma membrane peak because labelling of protoplasts with colloidal gold prior to disruption moved the 125I peak and the corresponding glucan synthetase II activity into denser regions of the gradient, leaving endoplasmic reticulum-contaminating IDPase (EC 3.1.3.31) and other glucan synthetase II peaks unmoved. Results are discussed in relation to various strategies of plasma membrane isolation. |
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Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase I activity. After sucrose density gradient centrifugation one of the several GS II activity peaks co-sedimented with the single radioactive particulate peak from [125I]-labelled protoplasts at 1.14 g ml−1. This peak is presumed to be the plasma membrane peak because labelling of protoplasts with colloidal gold prior to disruption moved the 125I peak and the corresponding glucan synthetase II activity into denser regions of the gradient, leaving endoplasmic reticulum-contaminating IDPase (EC 3.1.3.31) and other glucan synthetase II peaks unmoved. Results are discussed in relation to various strategies of plasma membrane isolation. |
| abstract_unstemmed |
Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase I activity. After sucrose density gradient centrifugation one of the several GS II activity peaks co-sedimented with the single radioactive particulate peak from [125I]-labelled protoplasts at 1.14 g ml−1. This peak is presumed to be the plasma membrane peak because labelling of protoplasts with colloidal gold prior to disruption moved the 125I peak and the corresponding glucan synthetase II activity into denser regions of the gradient, leaving endoplasmic reticulum-contaminating IDPase (EC 3.1.3.31) and other glucan synthetase II peaks unmoved. Results are discussed in relation to various strategies of plasma membrane isolation. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ240970659</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506080303.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120426s1986 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1399-3054.1986.tb02419.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ240970659</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Ostrowski, Elke D.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">The use of colloid gold labelling in the detection of plasma membrane from symbiotic and non-symbiotic Glycine max root cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1986</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Glycine max L. Merr. cv. Maple Arrow protoplasts were prepared from both tissue-cultured root cells and symbiotically-infected (fix+) nodule cells. Whilst both cell types showed glucan synthetase II (GS II; EC 2.4.1.29) activity, neither cell type, whole or gently disrupted, showed glucan synthetase I activity. After sucrose density gradient centrifugation one of the several GS II activity peaks co-sedimented with the single radioactive particulate peak from [125I]-labelled protoplasts at 1.14 g ml−1. This peak is presumed to be the plasma membrane peak because labelling of protoplasts with colloidal gold prior to disruption moved the 125I peak and the corresponding glucan synthetase II activity into denser regions of the gradient, leaving endoplasmic reticulum-contaminating IDPase (EC 3.1.3.31) and other glucan synthetase II peaks unmoved. Results are discussed in relation to various strategies of plasma membrane isolation.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Glucan synthetase</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mellor, Robert B.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Werner, Dietrich</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Physiologia plantarum</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1948</subfield><subfield code="g">66(1986), 2, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927738</subfield><subfield code="w">(DE-600)2020837-6</subfield><subfield code="x">1399-3054</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:66</subfield><subfield code="g">year:1986</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1399-3054.1986.tb02419.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">66</subfield><subfield code="j">1986</subfield><subfield code="e">2</subfield><subfield code="h">0</subfield></datafield></record></collection>
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7.399132 |