In vitro cytokine production and phenotype expression by blood mononuclear cellsfrom umbilical cords, children and adults
Age related differences in immunological reactions include variations in the in vitro functions of blood mononuclear cells (MNC). In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro prod...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Müller, K. [verfasserIn] Zak, M. [verfasserIn] Nielsen, S. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1996 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2007 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Pediatric allergy and immunology - Oxford [u.a.] : Wiley-Blackwell, 1990, 7(1996), 3, Seite 0 |
| Übergeordnetes Werk: |
volume:7 ; year:1996 ; number:3 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1111/j.1399-3038.1996.tb00118.x |
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| Katalog-ID: |
NLEJ241200407 |
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| 520 | |a Age related differences in immunological reactions include variations in the in vitro functions of blood mononuclear cells (MNC). In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro production of interleukin IL-6, tumour necrosis factor alpha (TNFα) and in-terferon gamma (IFNg) in neonates, children and adults. In cultures without added polyclonal activators IL-6 and TNFα levels in children were 3–6 times higher than those of umbilical cords and adults. However, using optimal in vitro stimulation (E. coli lipopolysaccharide (LPS), phytohaemmag-glutinin or pokeweed mitogen (PWM)) no significant differences in the levels of these cytokines were observed. The levels of IFNg in PWM driven cultures followed a different pattern with comparable levels in children and adults, and unmeasurable levels in cord blood MNC. Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD3, CD4, CD8, CD14, CD19, CD45RA, CD45RO, CD2, LFA-1, ICAM-1 and LFA-3. Correlation studies did not indicate that the observed differences in cytokine production could be ascribed to differences in the frequency of monocytes, T cells or B cells. The TNFα levels in suboptimally stimulated cultures correlated negatively with the expression of LFA-3 and positively with CD45RA. while IFNg correlated positively with CD2, LFA-1, CD45R0 and CD8.In conclusion, the study provides evidence of age related differences in the production of TNFα, IL-6 and IFNg among neonates, children and adults. These differences may to some extent be caused by differences in the expression of cell surface molecules involved in cellular interactions and signalling. | ||
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10.1111/j.1399-3038.1996.tb00118.x doi (DE-627)NLEJ241200407 DE-627 ger DE-627 rakwb Müller, K. verfasserin aut In vitro cytokine production and phenotype expression by blood mononuclear cellsfrom umbilical cords, children and adults Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Age related differences in immunological reactions include variations in the in vitro functions of blood mononuclear cells (MNC). In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro production of interleukin IL-6, tumour necrosis factor alpha (TNFα) and in-terferon gamma (IFNg) in neonates, children and adults. In cultures without added polyclonal activators IL-6 and TNFα levels in children were 3–6 times higher than those of umbilical cords and adults. However, using optimal in vitro stimulation (E. coli lipopolysaccharide (LPS), phytohaemmag-glutinin or pokeweed mitogen (PWM)) no significant differences in the levels of these cytokines were observed. The levels of IFNg in PWM driven cultures followed a different pattern with comparable levels in children and adults, and unmeasurable levels in cord blood MNC. Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD3, CD4, CD8, CD14, CD19, CD45RA, CD45RO, CD2, LFA-1, ICAM-1 and LFA-3. Correlation studies did not indicate that the observed differences in cytokine production could be ascribed to differences in the frequency of monocytes, T cells or B cells. The TNFα levels in suboptimally stimulated cultures correlated negatively with the expression of LFA-3 and positively with CD45RA. while IFNg correlated positively with CD2, LFA-1, CD45R0 and CD8.In conclusion, the study provides evidence of age related differences in the production of TNFα, IL-6 and IFNg among neonates, children and adults. These differences may to some extent be caused by differences in the expression of cell surface molecules involved in cellular interactions and signalling. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| interleukin-6 Zak, M. verfasserin aut Nielsen, S. verfasserin aut Pedersen, F. K. oth Nully, P. oth Bendtzen, K. oth In Pediatric allergy and immunology Oxford [u.a.] : Wiley-Blackwell, 1990 7(1996), 3, Seite 0 Online-Ressource (DE-627)NLEJ243926308 (DE-600)2008584-9 1399-3038 nnns volume:7 year:1996 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3038.1996.tb00118.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 7 1996 3 0 |
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10.1111/j.1399-3038.1996.tb00118.x doi (DE-627)NLEJ241200407 DE-627 ger DE-627 rakwb Müller, K. verfasserin aut In vitro cytokine production and phenotype expression by blood mononuclear cellsfrom umbilical cords, children and adults Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Age related differences in immunological reactions include variations in the in vitro functions of blood mononuclear cells (MNC). In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro production of interleukin IL-6, tumour necrosis factor alpha (TNFα) and in-terferon gamma (IFNg) in neonates, children and adults. In cultures without added polyclonal activators IL-6 and TNFα levels in children were 3–6 times higher than those of umbilical cords and adults. However, using optimal in vitro stimulation (E. coli lipopolysaccharide (LPS), phytohaemmag-glutinin or pokeweed mitogen (PWM)) no significant differences in the levels of these cytokines were observed. The levels of IFNg in PWM driven cultures followed a different pattern with comparable levels in children and adults, and unmeasurable levels in cord blood MNC. Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD3, CD4, CD8, CD14, CD19, CD45RA, CD45RO, CD2, LFA-1, ICAM-1 and LFA-3. Correlation studies did not indicate that the observed differences in cytokine production could be ascribed to differences in the frequency of monocytes, T cells or B cells. The TNFα levels in suboptimally stimulated cultures correlated negatively with the expression of LFA-3 and positively with CD45RA. while IFNg correlated positively with CD2, LFA-1, CD45R0 and CD8.In conclusion, the study provides evidence of age related differences in the production of TNFα, IL-6 and IFNg among neonates, children and adults. These differences may to some extent be caused by differences in the expression of cell surface molecules involved in cellular interactions and signalling. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| interleukin-6 Zak, M. verfasserin aut Nielsen, S. verfasserin aut Pedersen, F. K. oth Nully, P. oth Bendtzen, K. oth In Pediatric allergy and immunology Oxford [u.a.] : Wiley-Blackwell, 1990 7(1996), 3, Seite 0 Online-Ressource (DE-627)NLEJ243926308 (DE-600)2008584-9 1399-3038 nnns volume:7 year:1996 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3038.1996.tb00118.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 7 1996 3 0 |
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10.1111/j.1399-3038.1996.tb00118.x doi (DE-627)NLEJ241200407 DE-627 ger DE-627 rakwb Müller, K. verfasserin aut In vitro cytokine production and phenotype expression by blood mononuclear cellsfrom umbilical cords, children and adults Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Age related differences in immunological reactions include variations in the in vitro functions of blood mononuclear cells (MNC). In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro production of interleukin IL-6, tumour necrosis factor alpha (TNFα) and in-terferon gamma (IFNg) in neonates, children and adults. In cultures without added polyclonal activators IL-6 and TNFα levels in children were 3–6 times higher than those of umbilical cords and adults. However, using optimal in vitro stimulation (E. coli lipopolysaccharide (LPS), phytohaemmag-glutinin or pokeweed mitogen (PWM)) no significant differences in the levels of these cytokines were observed. The levels of IFNg in PWM driven cultures followed a different pattern with comparable levels in children and adults, and unmeasurable levels in cord blood MNC. Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD3, CD4, CD8, CD14, CD19, CD45RA, CD45RO, CD2, LFA-1, ICAM-1 and LFA-3. Correlation studies did not indicate that the observed differences in cytokine production could be ascribed to differences in the frequency of monocytes, T cells or B cells. The TNFα levels in suboptimally stimulated cultures correlated negatively with the expression of LFA-3 and positively with CD45RA. while IFNg correlated positively with CD2, LFA-1, CD45R0 and CD8.In conclusion, the study provides evidence of age related differences in the production of TNFα, IL-6 and IFNg among neonates, children and adults. These differences may to some extent be caused by differences in the expression of cell surface molecules involved in cellular interactions and signalling. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| interleukin-6 Zak, M. verfasserin aut Nielsen, S. verfasserin aut Pedersen, F. K. oth Nully, P. oth Bendtzen, K. oth In Pediatric allergy and immunology Oxford [u.a.] : Wiley-Blackwell, 1990 7(1996), 3, Seite 0 Online-Ressource (DE-627)NLEJ243926308 (DE-600)2008584-9 1399-3038 nnns volume:7 year:1996 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3038.1996.tb00118.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 7 1996 3 0 |
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10.1111/j.1399-3038.1996.tb00118.x doi (DE-627)NLEJ241200407 DE-627 ger DE-627 rakwb Müller, K. verfasserin aut In vitro cytokine production and phenotype expression by blood mononuclear cellsfrom umbilical cords, children and adults Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Age related differences in immunological reactions include variations in the in vitro functions of blood mononuclear cells (MNC). In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro production of interleukin IL-6, tumour necrosis factor alpha (TNFα) and in-terferon gamma (IFNg) in neonates, children and adults. In cultures without added polyclonal activators IL-6 and TNFα levels in children were 3–6 times higher than those of umbilical cords and adults. However, using optimal in vitro stimulation (E. coli lipopolysaccharide (LPS), phytohaemmag-glutinin or pokeweed mitogen (PWM)) no significant differences in the levels of these cytokines were observed. The levels of IFNg in PWM driven cultures followed a different pattern with comparable levels in children and adults, and unmeasurable levels in cord blood MNC. Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD3, CD4, CD8, CD14, CD19, CD45RA, CD45RO, CD2, LFA-1, ICAM-1 and LFA-3. Correlation studies did not indicate that the observed differences in cytokine production could be ascribed to differences in the frequency of monocytes, T cells or B cells. The TNFα levels in suboptimally stimulated cultures correlated negatively with the expression of LFA-3 and positively with CD45RA. while IFNg correlated positively with CD2, LFA-1, CD45R0 and CD8.In conclusion, the study provides evidence of age related differences in the production of TNFα, IL-6 and IFNg among neonates, children and adults. These differences may to some extent be caused by differences in the expression of cell surface molecules involved in cellular interactions and signalling. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| interleukin-6 Zak, M. verfasserin aut Nielsen, S. verfasserin aut Pedersen, F. K. oth Nully, P. oth Bendtzen, K. oth In Pediatric allergy and immunology Oxford [u.a.] : Wiley-Blackwell, 1990 7(1996), 3, Seite 0 Online-Ressource (DE-627)NLEJ243926308 (DE-600)2008584-9 1399-3038 nnns volume:7 year:1996 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3038.1996.tb00118.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 7 1996 3 0 |
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10.1111/j.1399-3038.1996.tb00118.x doi (DE-627)NLEJ241200407 DE-627 ger DE-627 rakwb Müller, K. verfasserin aut In vitro cytokine production and phenotype expression by blood mononuclear cellsfrom umbilical cords, children and adults Oxford, UK Blackwell Publishing Ltd 1996 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Age related differences in immunological reactions include variations in the in vitro functions of blood mononuclear cells (MNC). In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro production of interleukin IL-6, tumour necrosis factor alpha (TNFα) and in-terferon gamma (IFNg) in neonates, children and adults. In cultures without added polyclonal activators IL-6 and TNFα levels in children were 3–6 times higher than those of umbilical cords and adults. However, using optimal in vitro stimulation (E. coli lipopolysaccharide (LPS), phytohaemmag-glutinin or pokeweed mitogen (PWM)) no significant differences in the levels of these cytokines were observed. The levels of IFNg in PWM driven cultures followed a different pattern with comparable levels in children and adults, and unmeasurable levels in cord blood MNC. Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD3, CD4, CD8, CD14, CD19, CD45RA, CD45RO, CD2, LFA-1, ICAM-1 and LFA-3. Correlation studies did not indicate that the observed differences in cytokine production could be ascribed to differences in the frequency of monocytes, T cells or B cells. The TNFα levels in suboptimally stimulated cultures correlated negatively with the expression of LFA-3 and positively with CD45RA. while IFNg correlated positively with CD2, LFA-1, CD45R0 and CD8.In conclusion, the study provides evidence of age related differences in the production of TNFα, IL-6 and IFNg among neonates, children and adults. These differences may to some extent be caused by differences in the expression of cell surface molecules involved in cellular interactions and signalling. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| interleukin-6 Zak, M. verfasserin aut Nielsen, S. verfasserin aut Pedersen, F. K. oth Nully, P. oth Bendtzen, K. oth In Pediatric allergy and immunology Oxford [u.a.] : Wiley-Blackwell, 1990 7(1996), 3, Seite 0 Online-Ressource (DE-627)NLEJ243926308 (DE-600)2008584-9 1399-3038 nnns volume:7 year:1996 number:3 pages:0 http://dx.doi.org/10.1111/j.1399-3038.1996.tb00118.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 7 1996 3 0 |
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Müller, K. Zak, M. Nielsen, S. |
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Elektronische Aufsätze |
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Müller, K. |
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10.1111/j.1399-3038.1996.tb00118.x |
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verfasserin |
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in vitro cytokine production and phenotype expression by blood mononuclear cellsfrom umbilical cords, children and adults |
| title_auth |
In vitro cytokine production and phenotype expression by blood mononuclear cellsfrom umbilical cords, children and adults |
| abstract |
Age related differences in immunological reactions include variations in the in vitro functions of blood mononuclear cells (MNC). In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro production of interleukin IL-6, tumour necrosis factor alpha (TNFα) and in-terferon gamma (IFNg) in neonates, children and adults. In cultures without added polyclonal activators IL-6 and TNFα levels in children were 3–6 times higher than those of umbilical cords and adults. However, using optimal in vitro stimulation (E. coli lipopolysaccharide (LPS), phytohaemmag-glutinin or pokeweed mitogen (PWM)) no significant differences in the levels of these cytokines were observed. The levels of IFNg in PWM driven cultures followed a different pattern with comparable levels in children and adults, and unmeasurable levels in cord blood MNC. Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD3, CD4, CD8, CD14, CD19, CD45RA, CD45RO, CD2, LFA-1, ICAM-1 and LFA-3. Correlation studies did not indicate that the observed differences in cytokine production could be ascribed to differences in the frequency of monocytes, T cells or B cells. The TNFα levels in suboptimally stimulated cultures correlated negatively with the expression of LFA-3 and positively with CD45RA. while IFNg correlated positively with CD2, LFA-1, CD45R0 and CD8.In conclusion, the study provides evidence of age related differences in the production of TNFα, IL-6 and IFNg among neonates, children and adults. These differences may to some extent be caused by differences in the expression of cell surface molecules involved in cellular interactions and signalling. |
| abstractGer |
Age related differences in immunological reactions include variations in the in vitro functions of blood mononuclear cells (MNC). In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro production of interleukin IL-6, tumour necrosis factor alpha (TNFα) and in-terferon gamma (IFNg) in neonates, children and adults. In cultures without added polyclonal activators IL-6 and TNFα levels in children were 3–6 times higher than those of umbilical cords and adults. However, using optimal in vitro stimulation (E. coli lipopolysaccharide (LPS), phytohaemmag-glutinin or pokeweed mitogen (PWM)) no significant differences in the levels of these cytokines were observed. The levels of IFNg in PWM driven cultures followed a different pattern with comparable levels in children and adults, and unmeasurable levels in cord blood MNC. Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD3, CD4, CD8, CD14, CD19, CD45RA, CD45RO, CD2, LFA-1, ICAM-1 and LFA-3. Correlation studies did not indicate that the observed differences in cytokine production could be ascribed to differences in the frequency of monocytes, T cells or B cells. The TNFα levels in suboptimally stimulated cultures correlated negatively with the expression of LFA-3 and positively with CD45RA. while IFNg correlated positively with CD2, LFA-1, CD45R0 and CD8.In conclusion, the study provides evidence of age related differences in the production of TNFα, IL-6 and IFNg among neonates, children and adults. These differences may to some extent be caused by differences in the expression of cell surface molecules involved in cellular interactions and signalling. |
| abstract_unstemmed |
Age related differences in immunological reactions include variations in the in vitro functions of blood mononuclear cells (MNC). In an attempt to understand the mechanism behind these differences we examined age related differences in the phenotype profiles of MNC in parallel with the in vitro production of interleukin IL-6, tumour necrosis factor alpha (TNFα) and in-terferon gamma (IFNg) in neonates, children and adults. In cultures without added polyclonal activators IL-6 and TNFα levels in children were 3–6 times higher than those of umbilical cords and adults. However, using optimal in vitro stimulation (E. coli lipopolysaccharide (LPS), phytohaemmag-glutinin or pokeweed mitogen (PWM)) no significant differences in the levels of these cytokines were observed. The levels of IFNg in PWM driven cultures followed a different pattern with comparable levels in children and adults, and unmeasurable levels in cord blood MNC. Flow cytometry analysis of the phenotypic distribution of MNC revealed age related differences in the expression of CD3, CD4, CD8, CD14, CD19, CD45RA, CD45RO, CD2, LFA-1, ICAM-1 and LFA-3. Correlation studies did not indicate that the observed differences in cytokine production could be ascribed to differences in the frequency of monocytes, T cells or B cells. The TNFα levels in suboptimally stimulated cultures correlated negatively with the expression of LFA-3 and positively with CD45RA. while IFNg correlated positively with CD2, LFA-1, CD45R0 and CD8.In conclusion, the study provides evidence of age related differences in the production of TNFα, IL-6 and IFNg among neonates, children and adults. These differences may to some extent be caused by differences in the expression of cell surface molecules involved in cellular interactions and signalling. |
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In vitro cytokine production and phenotype expression by blood mononuclear cellsfrom umbilical cords, children and adults |
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