Platelets Contain Interleukin-1 Alpha and Beta which are Detectable on the Cell Surface After Activation
Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activat...
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| Autor*in: |
SEDLMAYR, P. [verfasserIn] BLASCHITZ, A. [verfasserIn] WILDERS-TRUSCHNIG, M. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1995 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2008 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Scandinavian journal of immunology - Oxford [u.a.] : Wiley-Blackwell, 1972, 42(1995), 2, Seite 0 |
| Übergeordnetes Werk: |
volume:42 ; year:1995 ; number:2 ; pages:0 |
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| DOI / URN: |
10.1111/j.1365-3083.1995.tb03647.x |
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| 520 | |a Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activated platelets. Immunogold-silver staining of the cell surface of activated platelets as well as pre-embedding antibody treatment of platelets revealed the presence of IL-1 (α and β) in low density on the surface of intact cells in contrast to distinct enrichment in the cytoplasma of damaged platelets. Fibrin fibres present between cells indicated adsorbance of IL-1. There was also weak binding of anti-IL-1α to the surface of thrombin-activated platelets as shown by flow cytometry. Following activation there appears to be some transfer of IL-1 onto the cell surface of activated cells, the bulk of the cytokine, however, is probably not released prior to platelet disintegration. In summary, we present evidence for the presence of both IL-1α and IL-1β in resting and activated platelets without being able to demonstrate localization of the cytokines to specific subcellular structures. | ||
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10.1111/j.1365-3083.1995.tb03647.x doi (DE-627)NLEJ241944163 DE-627 ger DE-627 rakwb SEDLMAYR, P. verfasserin aut Platelets Contain Interleukin-1 Alpha and Beta which are Detectable on the Cell Surface After Activation Oxford, UK Blackwell Publishing Ltd 1995 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activated platelets. Immunogold-silver staining of the cell surface of activated platelets as well as pre-embedding antibody treatment of platelets revealed the presence of IL-1 (α and β) in low density on the surface of intact cells in contrast to distinct enrichment in the cytoplasma of damaged platelets. Fibrin fibres present between cells indicated adsorbance of IL-1. There was also weak binding of anti-IL-1α to the surface of thrombin-activated platelets as shown by flow cytometry. Following activation there appears to be some transfer of IL-1 onto the cell surface of activated cells, the bulk of the cytokine, however, is probably not released prior to platelet disintegration. In summary, we present evidence for the presence of both IL-1α and IL-1β in resting and activated platelets without being able to demonstrate localization of the cytokines to specific subcellular structures. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| BLASCHITZ, A. verfasserin aut WILDERS-TRUSCHNIG, M. verfasserin aut TIRAN, A. oth DOHR, G. oth In Scandinavian journal of immunology Oxford [u.a.] : Wiley-Blackwell, 1972 42(1995), 2, Seite 0 Online-Ressource (DE-627)NLEJ24392724X (DE-600)2020954-X 1365-3083 nnns volume:42 year:1995 number:2 pages:0 http://dx.doi.org/10.1111/j.1365-3083.1995.tb03647.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 42 1995 2 0 |
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10.1111/j.1365-3083.1995.tb03647.x doi (DE-627)NLEJ241944163 DE-627 ger DE-627 rakwb SEDLMAYR, P. verfasserin aut Platelets Contain Interleukin-1 Alpha and Beta which are Detectable on the Cell Surface After Activation Oxford, UK Blackwell Publishing Ltd 1995 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activated platelets. Immunogold-silver staining of the cell surface of activated platelets as well as pre-embedding antibody treatment of platelets revealed the presence of IL-1 (α and β) in low density on the surface of intact cells in contrast to distinct enrichment in the cytoplasma of damaged platelets. Fibrin fibres present between cells indicated adsorbance of IL-1. There was also weak binding of anti-IL-1α to the surface of thrombin-activated platelets as shown by flow cytometry. Following activation there appears to be some transfer of IL-1 onto the cell surface of activated cells, the bulk of the cytokine, however, is probably not released prior to platelet disintegration. In summary, we present evidence for the presence of both IL-1α and IL-1β in resting and activated platelets without being able to demonstrate localization of the cytokines to specific subcellular structures. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| BLASCHITZ, A. verfasserin aut WILDERS-TRUSCHNIG, M. verfasserin aut TIRAN, A. oth DOHR, G. oth In Scandinavian journal of immunology Oxford [u.a.] : Wiley-Blackwell, 1972 42(1995), 2, Seite 0 Online-Ressource (DE-627)NLEJ24392724X (DE-600)2020954-X 1365-3083 nnns volume:42 year:1995 number:2 pages:0 http://dx.doi.org/10.1111/j.1365-3083.1995.tb03647.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 42 1995 2 0 |
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10.1111/j.1365-3083.1995.tb03647.x doi (DE-627)NLEJ241944163 DE-627 ger DE-627 rakwb SEDLMAYR, P. verfasserin aut Platelets Contain Interleukin-1 Alpha and Beta which are Detectable on the Cell Surface After Activation Oxford, UK Blackwell Publishing Ltd 1995 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activated platelets. Immunogold-silver staining of the cell surface of activated platelets as well as pre-embedding antibody treatment of platelets revealed the presence of IL-1 (α and β) in low density on the surface of intact cells in contrast to distinct enrichment in the cytoplasma of damaged platelets. Fibrin fibres present between cells indicated adsorbance of IL-1. There was also weak binding of anti-IL-1α to the surface of thrombin-activated platelets as shown by flow cytometry. Following activation there appears to be some transfer of IL-1 onto the cell surface of activated cells, the bulk of the cytokine, however, is probably not released prior to platelet disintegration. In summary, we present evidence for the presence of both IL-1α and IL-1β in resting and activated platelets without being able to demonstrate localization of the cytokines to specific subcellular structures. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| BLASCHITZ, A. verfasserin aut WILDERS-TRUSCHNIG, M. verfasserin aut TIRAN, A. oth DOHR, G. oth In Scandinavian journal of immunology Oxford [u.a.] : Wiley-Blackwell, 1972 42(1995), 2, Seite 0 Online-Ressource (DE-627)NLEJ24392724X (DE-600)2020954-X 1365-3083 nnns volume:42 year:1995 number:2 pages:0 http://dx.doi.org/10.1111/j.1365-3083.1995.tb03647.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 42 1995 2 0 |
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10.1111/j.1365-3083.1995.tb03647.x doi (DE-627)NLEJ241944163 DE-627 ger DE-627 rakwb SEDLMAYR, P. verfasserin aut Platelets Contain Interleukin-1 Alpha and Beta which are Detectable on the Cell Surface After Activation Oxford, UK Blackwell Publishing Ltd 1995 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activated platelets. Immunogold-silver staining of the cell surface of activated platelets as well as pre-embedding antibody treatment of platelets revealed the presence of IL-1 (α and β) in low density on the surface of intact cells in contrast to distinct enrichment in the cytoplasma of damaged platelets. Fibrin fibres present between cells indicated adsorbance of IL-1. There was also weak binding of anti-IL-1α to the surface of thrombin-activated platelets as shown by flow cytometry. Following activation there appears to be some transfer of IL-1 onto the cell surface of activated cells, the bulk of the cytokine, however, is probably not released prior to platelet disintegration. In summary, we present evidence for the presence of both IL-1α and IL-1β in resting and activated platelets without being able to demonstrate localization of the cytokines to specific subcellular structures. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| BLASCHITZ, A. verfasserin aut WILDERS-TRUSCHNIG, M. verfasserin aut TIRAN, A. oth DOHR, G. oth In Scandinavian journal of immunology Oxford [u.a.] : Wiley-Blackwell, 1972 42(1995), 2, Seite 0 Online-Ressource (DE-627)NLEJ24392724X (DE-600)2020954-X 1365-3083 nnns volume:42 year:1995 number:2 pages:0 http://dx.doi.org/10.1111/j.1365-3083.1995.tb03647.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 42 1995 2 0 |
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10.1111/j.1365-3083.1995.tb03647.x doi (DE-627)NLEJ241944163 DE-627 ger DE-627 rakwb SEDLMAYR, P. verfasserin aut Platelets Contain Interleukin-1 Alpha and Beta which are Detectable on the Cell Surface After Activation Oxford, UK Blackwell Publishing Ltd 1995 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activated platelets. Immunogold-silver staining of the cell surface of activated platelets as well as pre-embedding antibody treatment of platelets revealed the presence of IL-1 (α and β) in low density on the surface of intact cells in contrast to distinct enrichment in the cytoplasma of damaged platelets. Fibrin fibres present between cells indicated adsorbance of IL-1. There was also weak binding of anti-IL-1α to the surface of thrombin-activated platelets as shown by flow cytometry. Following activation there appears to be some transfer of IL-1 onto the cell surface of activated cells, the bulk of the cytokine, however, is probably not released prior to platelet disintegration. In summary, we present evidence for the presence of both IL-1α and IL-1β in resting and activated platelets without being able to demonstrate localization of the cytokines to specific subcellular structures. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| BLASCHITZ, A. verfasserin aut WILDERS-TRUSCHNIG, M. verfasserin aut TIRAN, A. oth DOHR, G. oth In Scandinavian journal of immunology Oxford [u.a.] : Wiley-Blackwell, 1972 42(1995), 2, Seite 0 Online-Ressource (DE-627)NLEJ24392724X (DE-600)2020954-X 1365-3083 nnns volume:42 year:1995 number:2 pages:0 http://dx.doi.org/10.1111/j.1365-3083.1995.tb03647.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 42 1995 2 0 |
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Platelets Contain Interleukin-1 Alpha and Beta which are Detectable on the Cell Surface After Activation |
| abstract |
Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activated platelets. Immunogold-silver staining of the cell surface of activated platelets as well as pre-embedding antibody treatment of platelets revealed the presence of IL-1 (α and β) in low density on the surface of intact cells in contrast to distinct enrichment in the cytoplasma of damaged platelets. Fibrin fibres present between cells indicated adsorbance of IL-1. There was also weak binding of anti-IL-1α to the surface of thrombin-activated platelets as shown by flow cytometry. Following activation there appears to be some transfer of IL-1 onto the cell surface of activated cells, the bulk of the cytokine, however, is probably not released prior to platelet disintegration. In summary, we present evidence for the presence of both IL-1α and IL-1β in resting and activated platelets without being able to demonstrate localization of the cytokines to specific subcellular structures. |
| abstractGer |
Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activated platelets. Immunogold-silver staining of the cell surface of activated platelets as well as pre-embedding antibody treatment of platelets revealed the presence of IL-1 (α and β) in low density on the surface of intact cells in contrast to distinct enrichment in the cytoplasma of damaged platelets. Fibrin fibres present between cells indicated adsorbance of IL-1. There was also weak binding of anti-IL-1α to the surface of thrombin-activated platelets as shown by flow cytometry. Following activation there appears to be some transfer of IL-1 onto the cell surface of activated cells, the bulk of the cytokine, however, is probably not released prior to platelet disintegration. In summary, we present evidence for the presence of both IL-1α and IL-1β in resting and activated platelets without being able to demonstrate localization of the cytokines to specific subcellular structures. |
| abstract_unstemmed |
Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activated platelets. Immunogold-silver staining of the cell surface of activated platelets as well as pre-embedding antibody treatment of platelets revealed the presence of IL-1 (α and β) in low density on the surface of intact cells in contrast to distinct enrichment in the cytoplasma of damaged platelets. Fibrin fibres present between cells indicated adsorbance of IL-1. There was also weak binding of anti-IL-1α to the surface of thrombin-activated platelets as shown by flow cytometry. Following activation there appears to be some transfer of IL-1 onto the cell surface of activated cells, the bulk of the cytokine, however, is probably not released prior to platelet disintegration. In summary, we present evidence for the presence of both IL-1α and IL-1β in resting and activated platelets without being able to demonstrate localization of the cytokines to specific subcellular structures. |
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Platelets Contain Interleukin-1 Alpha and Beta which are Detectable on the Cell Surface After Activation |
| url |
http://dx.doi.org/10.1111/j.1365-3083.1995.tb03647.x |
| remote_bool |
true |
| author2 |
BLASCHITZ, A. WILDERS-TRUSCHNIG, M. TIRAN, A. DOHR, G. |
| author2Str |
BLASCHITZ, A. WILDERS-TRUSCHNIG, M. TIRAN, A. DOHR, G. |
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NLEJ24392724X |
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z |
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oth oth |
| doi_str |
10.1111/j.1365-3083.1995.tb03647.x |
| up_date |
2024-07-06T00:19:52.798Z |
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1803786851765452800 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ241944163</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707144105.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120427s1995 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1365-3083.1995.tb03647.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ241944163</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">SEDLMAYR, P.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Platelets Contain Interleukin-1 Alpha and Beta which are Detectable on the Cell Surface After Activation</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1995</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Activated platelets have been shown previously to exhibit membrane-bound IL-1 bioactivity, which leads to the question of localization of the cytokine in platelets. Using immunocytological and flow cytometric techniques, we found IL-la and IL-Iβ in the cytoplasma of both resting and thrombin-activated platelets. Immunogold-silver staining of the cell surface of activated platelets as well as pre-embedding antibody treatment of platelets revealed the presence of IL-1 (α and β) in low density on the surface of intact cells in contrast to distinct enrichment in the cytoplasma of damaged platelets. Fibrin fibres present between cells indicated adsorbance of IL-1. There was also weak binding of anti-IL-1α to the surface of thrombin-activated platelets as shown by flow cytometry. Following activation there appears to be some transfer of IL-1 onto the cell surface of activated cells, the bulk of the cytokine, however, is probably not released prior to platelet disintegration. 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| score |
7.400403 |