The Influence of Antigen Density and a Comparison of IgG and IgM Antibodies in the Anti-Complementary Modulation of Lymphocytic Surface Immunoglobulin
Experiments were carried out to determine the influence of antigen density on anti-complementary modulation, defined here as the conferring by anti-immunoglobulin (Ig) of resistance to lysis of guinea pig L2C leukaemic lymphocytes through anti-Ig plus syngeneic complement (C). Populations of leukaem...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
GORDON, J. [verfasserIn] ANDERSON, V. A. [verfasserIn] ROBINSON, D. S. F. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|
| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1982 |
|---|
| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
|---|---|
| Übergeordnetes Werk: |
In: Scandinavian journal of immunology - Oxford [u.a.] : Wiley-Blackwell, 1972, 15(1982), 2, Seite 0 |
| Übergeordnetes Werk: |
volume:15 ; year:1982 ; number:2 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1111/j.1365-3083.1982.tb00635.x |
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| 520 | |a Experiments were carried out to determine the influence of antigen density on anti-complementary modulation, defined here as the conferring by anti-immunoglobulin (Ig) of resistance to lysis of guinea pig L2C leukaemic lymphocytes through anti-Ig plus syngeneic complement (C). Populations of leukaemic cells, varied widely in their quantitative expression of surface membrane Ig as judged by the binding to cells of 125I-labelled Fab’ fragments from anti-Ig, and a good correlation between the bulk antigen density and the percentage of cells lysed by anti-Ig plus C was obtained (P=0.02). In the presence of 10mm sodium azide, which has been shown to diminish ihe modulation occuring during simultaneous incubations with anti-Ig and C, this correlation was even stronger (P < 0.001). No zone could be defined in which the level of surface Ig expression was sufficient to serve complement lysis but too low for modulation. Furthermore, both the degrees and rates of modulation occurring on incubation with antibody at 37°C, either before C addition or in the presenee of lytic C, were similar for populations of high, low, or intermediate antigen density. Separation of cells by their size or density failed to yield populations differing in either their susceptibility to humoral killing through anti-Ig or their modulating capacity. IgG and IgM antibodies to the L2C cell surfce Ig evoked similar levels of killing with syngeneic C, and when compared for their ability to promote anti-complementary modulation, no difference was revealed in either the rule or degree of modulation occurring during incubations at 37°C with the two isotypes. The findings are discussed with particular reference to observations on the modulation of mouse thymus leukaemia (TL) antigens. | ||
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10.1111/j.1365-3083.1982.tb00635.x doi (DE-627)NLEJ241972302 DE-627 ger DE-627 rakwb GORDON, J. verfasserin aut The Influence of Antigen Density and a Comparison of IgG and IgM Antibodies in the Anti-Complementary Modulation of Lymphocytic Surface Immunoglobulin Oxford, UK Blackwell Publishing Ltd 1982 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Experiments were carried out to determine the influence of antigen density on anti-complementary modulation, defined here as the conferring by anti-immunoglobulin (Ig) of resistance to lysis of guinea pig L2C leukaemic lymphocytes through anti-Ig plus syngeneic complement (C). Populations of leukaemic cells, varied widely in their quantitative expression of surface membrane Ig as judged by the binding to cells of 125I-labelled Fab’ fragments from anti-Ig, and a good correlation between the bulk antigen density and the percentage of cells lysed by anti-Ig plus C was obtained (P=0.02). In the presence of 10mm sodium azide, which has been shown to diminish ihe modulation occuring during simultaneous incubations with anti-Ig and C, this correlation was even stronger (P < 0.001). No zone could be defined in which the level of surface Ig expression was sufficient to serve complement lysis but too low for modulation. Furthermore, both the degrees and rates of modulation occurring on incubation with antibody at 37°C, either before C addition or in the presenee of lytic C, were similar for populations of high, low, or intermediate antigen density. Separation of cells by their size or density failed to yield populations differing in either their susceptibility to humoral killing through anti-Ig or their modulating capacity. IgG and IgM antibodies to the L2C cell surfce Ig evoked similar levels of killing with syngeneic C, and when compared for their ability to promote anti-complementary modulation, no difference was revealed in either the rule or degree of modulation occurring during incubations at 37°C with the two isotypes. The findings are discussed with particular reference to observations on the modulation of mouse thymus leukaemia (TL) antigens. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| ANDERSON, V. A. verfasserin aut ROBINSON, D. S. F. verfasserin aut STEVENSON, G. T. oth In Scandinavian journal of immunology Oxford [u.a.] : Wiley-Blackwell, 1972 15(1982), 2, Seite 0 Online-Ressource (DE-627)NLEJ24392724X (DE-600)2020954-X 1365-3083 nnns volume:15 year:1982 number:2 pages:0 http://dx.doi.org/10.1111/j.1365-3083.1982.tb00635.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 15 1982 2 0 |
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10.1111/j.1365-3083.1982.tb00635.x doi (DE-627)NLEJ241972302 DE-627 ger DE-627 rakwb GORDON, J. verfasserin aut The Influence of Antigen Density and a Comparison of IgG and IgM Antibodies in the Anti-Complementary Modulation of Lymphocytic Surface Immunoglobulin Oxford, UK Blackwell Publishing Ltd 1982 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Experiments were carried out to determine the influence of antigen density on anti-complementary modulation, defined here as the conferring by anti-immunoglobulin (Ig) of resistance to lysis of guinea pig L2C leukaemic lymphocytes through anti-Ig plus syngeneic complement (C). Populations of leukaemic cells, varied widely in their quantitative expression of surface membrane Ig as judged by the binding to cells of 125I-labelled Fab’ fragments from anti-Ig, and a good correlation between the bulk antigen density and the percentage of cells lysed by anti-Ig plus C was obtained (P=0.02). In the presence of 10mm sodium azide, which has been shown to diminish ihe modulation occuring during simultaneous incubations with anti-Ig and C, this correlation was even stronger (P < 0.001). No zone could be defined in which the level of surface Ig expression was sufficient to serve complement lysis but too low for modulation. Furthermore, both the degrees and rates of modulation occurring on incubation with antibody at 37°C, either before C addition or in the presenee of lytic C, were similar for populations of high, low, or intermediate antigen density. Separation of cells by their size or density failed to yield populations differing in either their susceptibility to humoral killing through anti-Ig or their modulating capacity. IgG and IgM antibodies to the L2C cell surfce Ig evoked similar levels of killing with syngeneic C, and when compared for their ability to promote anti-complementary modulation, no difference was revealed in either the rule or degree of modulation occurring during incubations at 37°C with the two isotypes. The findings are discussed with particular reference to observations on the modulation of mouse thymus leukaemia (TL) antigens. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| ANDERSON, V. A. verfasserin aut ROBINSON, D. S. F. verfasserin aut STEVENSON, G. T. oth In Scandinavian journal of immunology Oxford [u.a.] : Wiley-Blackwell, 1972 15(1982), 2, Seite 0 Online-Ressource (DE-627)NLEJ24392724X (DE-600)2020954-X 1365-3083 nnns volume:15 year:1982 number:2 pages:0 http://dx.doi.org/10.1111/j.1365-3083.1982.tb00635.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 15 1982 2 0 |
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10.1111/j.1365-3083.1982.tb00635.x doi (DE-627)NLEJ241972302 DE-627 ger DE-627 rakwb GORDON, J. verfasserin aut The Influence of Antigen Density and a Comparison of IgG and IgM Antibodies in the Anti-Complementary Modulation of Lymphocytic Surface Immunoglobulin Oxford, UK Blackwell Publishing Ltd 1982 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Experiments were carried out to determine the influence of antigen density on anti-complementary modulation, defined here as the conferring by anti-immunoglobulin (Ig) of resistance to lysis of guinea pig L2C leukaemic lymphocytes through anti-Ig plus syngeneic complement (C). Populations of leukaemic cells, varied widely in their quantitative expression of surface membrane Ig as judged by the binding to cells of 125I-labelled Fab’ fragments from anti-Ig, and a good correlation between the bulk antigen density and the percentage of cells lysed by anti-Ig plus C was obtained (P=0.02). In the presence of 10mm sodium azide, which has been shown to diminish ihe modulation occuring during simultaneous incubations with anti-Ig and C, this correlation was even stronger (P < 0.001). No zone could be defined in which the level of surface Ig expression was sufficient to serve complement lysis but too low for modulation. Furthermore, both the degrees and rates of modulation occurring on incubation with antibody at 37°C, either before C addition or in the presenee of lytic C, were similar for populations of high, low, or intermediate antigen density. Separation of cells by their size or density failed to yield populations differing in either their susceptibility to humoral killing through anti-Ig or their modulating capacity. IgG and IgM antibodies to the L2C cell surfce Ig evoked similar levels of killing with syngeneic C, and when compared for their ability to promote anti-complementary modulation, no difference was revealed in either the rule or degree of modulation occurring during incubations at 37°C with the two isotypes. The findings are discussed with particular reference to observations on the modulation of mouse thymus leukaemia (TL) antigens. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| ANDERSON, V. A. verfasserin aut ROBINSON, D. S. F. verfasserin aut STEVENSON, G. T. oth In Scandinavian journal of immunology Oxford [u.a.] : Wiley-Blackwell, 1972 15(1982), 2, Seite 0 Online-Ressource (DE-627)NLEJ24392724X (DE-600)2020954-X 1365-3083 nnns volume:15 year:1982 number:2 pages:0 http://dx.doi.org/10.1111/j.1365-3083.1982.tb00635.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 15 1982 2 0 |
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10.1111/j.1365-3083.1982.tb00635.x doi (DE-627)NLEJ241972302 DE-627 ger DE-627 rakwb GORDON, J. verfasserin aut The Influence of Antigen Density and a Comparison of IgG and IgM Antibodies in the Anti-Complementary Modulation of Lymphocytic Surface Immunoglobulin Oxford, UK Blackwell Publishing Ltd 1982 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Experiments were carried out to determine the influence of antigen density on anti-complementary modulation, defined here as the conferring by anti-immunoglobulin (Ig) of resistance to lysis of guinea pig L2C leukaemic lymphocytes through anti-Ig plus syngeneic complement (C). Populations of leukaemic cells, varied widely in their quantitative expression of surface membrane Ig as judged by the binding to cells of 125I-labelled Fab’ fragments from anti-Ig, and a good correlation between the bulk antigen density and the percentage of cells lysed by anti-Ig plus C was obtained (P=0.02). In the presence of 10mm sodium azide, which has been shown to diminish ihe modulation occuring during simultaneous incubations with anti-Ig and C, this correlation was even stronger (P < 0.001). No zone could be defined in which the level of surface Ig expression was sufficient to serve complement lysis but too low for modulation. Furthermore, both the degrees and rates of modulation occurring on incubation with antibody at 37°C, either before C addition or in the presenee of lytic C, were similar for populations of high, low, or intermediate antigen density. Separation of cells by their size or density failed to yield populations differing in either their susceptibility to humoral killing through anti-Ig or their modulating capacity. IgG and IgM antibodies to the L2C cell surfce Ig evoked similar levels of killing with syngeneic C, and when compared for their ability to promote anti-complementary modulation, no difference was revealed in either the rule or degree of modulation occurring during incubations at 37°C with the two isotypes. The findings are discussed with particular reference to observations on the modulation of mouse thymus leukaemia (TL) antigens. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| ANDERSON, V. A. verfasserin aut ROBINSON, D. S. F. verfasserin aut STEVENSON, G. T. oth In Scandinavian journal of immunology Oxford [u.a.] : Wiley-Blackwell, 1972 15(1982), 2, Seite 0 Online-Ressource (DE-627)NLEJ24392724X (DE-600)2020954-X 1365-3083 nnns volume:15 year:1982 number:2 pages:0 http://dx.doi.org/10.1111/j.1365-3083.1982.tb00635.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 15 1982 2 0 |
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10.1111/j.1365-3083.1982.tb00635.x doi (DE-627)NLEJ241972302 DE-627 ger DE-627 rakwb GORDON, J. verfasserin aut The Influence of Antigen Density and a Comparison of IgG and IgM Antibodies in the Anti-Complementary Modulation of Lymphocytic Surface Immunoglobulin Oxford, UK Blackwell Publishing Ltd 1982 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Experiments were carried out to determine the influence of antigen density on anti-complementary modulation, defined here as the conferring by anti-immunoglobulin (Ig) of resistance to lysis of guinea pig L2C leukaemic lymphocytes through anti-Ig plus syngeneic complement (C). Populations of leukaemic cells, varied widely in their quantitative expression of surface membrane Ig as judged by the binding to cells of 125I-labelled Fab’ fragments from anti-Ig, and a good correlation between the bulk antigen density and the percentage of cells lysed by anti-Ig plus C was obtained (P=0.02). In the presence of 10mm sodium azide, which has been shown to diminish ihe modulation occuring during simultaneous incubations with anti-Ig and C, this correlation was even stronger (P < 0.001). No zone could be defined in which the level of surface Ig expression was sufficient to serve complement lysis but too low for modulation. Furthermore, both the degrees and rates of modulation occurring on incubation with antibody at 37°C, either before C addition or in the presenee of lytic C, were similar for populations of high, low, or intermediate antigen density. Separation of cells by their size or density failed to yield populations differing in either their susceptibility to humoral killing through anti-Ig or their modulating capacity. IgG and IgM antibodies to the L2C cell surfce Ig evoked similar levels of killing with syngeneic C, and when compared for their ability to promote anti-complementary modulation, no difference was revealed in either the rule or degree of modulation occurring during incubations at 37°C with the two isotypes. The findings are discussed with particular reference to observations on the modulation of mouse thymus leukaemia (TL) antigens. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| ANDERSON, V. A. verfasserin aut ROBINSON, D. S. F. verfasserin aut STEVENSON, G. T. oth In Scandinavian journal of immunology Oxford [u.a.] : Wiley-Blackwell, 1972 15(1982), 2, Seite 0 Online-Ressource (DE-627)NLEJ24392724X (DE-600)2020954-X 1365-3083 nnns volume:15 year:1982 number:2 pages:0 http://dx.doi.org/10.1111/j.1365-3083.1982.tb00635.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 15 1982 2 0 |
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the influence of antigen density and a comparison of igg and igm antibodies in the anti-complementary modulation of lymphocytic surface immunoglobulin |
| title_auth |
The Influence of Antigen Density and a Comparison of IgG and IgM Antibodies in the Anti-Complementary Modulation of Lymphocytic Surface Immunoglobulin |
| abstract |
Experiments were carried out to determine the influence of antigen density on anti-complementary modulation, defined here as the conferring by anti-immunoglobulin (Ig) of resistance to lysis of guinea pig L2C leukaemic lymphocytes through anti-Ig plus syngeneic complement (C). Populations of leukaemic cells, varied widely in their quantitative expression of surface membrane Ig as judged by the binding to cells of 125I-labelled Fab’ fragments from anti-Ig, and a good correlation between the bulk antigen density and the percentage of cells lysed by anti-Ig plus C was obtained (P=0.02). In the presence of 10mm sodium azide, which has been shown to diminish ihe modulation occuring during simultaneous incubations with anti-Ig and C, this correlation was even stronger (P < 0.001). No zone could be defined in which the level of surface Ig expression was sufficient to serve complement lysis but too low for modulation. Furthermore, both the degrees and rates of modulation occurring on incubation with antibody at 37°C, either before C addition or in the presenee of lytic C, were similar for populations of high, low, or intermediate antigen density. Separation of cells by their size or density failed to yield populations differing in either their susceptibility to humoral killing through anti-Ig or their modulating capacity. IgG and IgM antibodies to the L2C cell surfce Ig evoked similar levels of killing with syngeneic C, and when compared for their ability to promote anti-complementary modulation, no difference was revealed in either the rule or degree of modulation occurring during incubations at 37°C with the two isotypes. The findings are discussed with particular reference to observations on the modulation of mouse thymus leukaemia (TL) antigens. |
| abstractGer |
Experiments were carried out to determine the influence of antigen density on anti-complementary modulation, defined here as the conferring by anti-immunoglobulin (Ig) of resistance to lysis of guinea pig L2C leukaemic lymphocytes through anti-Ig plus syngeneic complement (C). Populations of leukaemic cells, varied widely in their quantitative expression of surface membrane Ig as judged by the binding to cells of 125I-labelled Fab’ fragments from anti-Ig, and a good correlation between the bulk antigen density and the percentage of cells lysed by anti-Ig plus C was obtained (P=0.02). In the presence of 10mm sodium azide, which has been shown to diminish ihe modulation occuring during simultaneous incubations with anti-Ig and C, this correlation was even stronger (P < 0.001). No zone could be defined in which the level of surface Ig expression was sufficient to serve complement lysis but too low for modulation. Furthermore, both the degrees and rates of modulation occurring on incubation with antibody at 37°C, either before C addition or in the presenee of lytic C, were similar for populations of high, low, or intermediate antigen density. Separation of cells by their size or density failed to yield populations differing in either their susceptibility to humoral killing through anti-Ig or their modulating capacity. IgG and IgM antibodies to the L2C cell surfce Ig evoked similar levels of killing with syngeneic C, and when compared for their ability to promote anti-complementary modulation, no difference was revealed in either the rule or degree of modulation occurring during incubations at 37°C with the two isotypes. The findings are discussed with particular reference to observations on the modulation of mouse thymus leukaemia (TL) antigens. |
| abstract_unstemmed |
Experiments were carried out to determine the influence of antigen density on anti-complementary modulation, defined here as the conferring by anti-immunoglobulin (Ig) of resistance to lysis of guinea pig L2C leukaemic lymphocytes through anti-Ig plus syngeneic complement (C). Populations of leukaemic cells, varied widely in their quantitative expression of surface membrane Ig as judged by the binding to cells of 125I-labelled Fab’ fragments from anti-Ig, and a good correlation between the bulk antigen density and the percentage of cells lysed by anti-Ig plus C was obtained (P=0.02). In the presence of 10mm sodium azide, which has been shown to diminish ihe modulation occuring during simultaneous incubations with anti-Ig and C, this correlation was even stronger (P < 0.001). No zone could be defined in which the level of surface Ig expression was sufficient to serve complement lysis but too low for modulation. Furthermore, both the degrees and rates of modulation occurring on incubation with antibody at 37°C, either before C addition or in the presenee of lytic C, were similar for populations of high, low, or intermediate antigen density. Separation of cells by their size or density failed to yield populations differing in either their susceptibility to humoral killing through anti-Ig or their modulating capacity. IgG and IgM antibodies to the L2C cell surfce Ig evoked similar levels of killing with syngeneic C, and when compared for their ability to promote anti-complementary modulation, no difference was revealed in either the rule or degree of modulation occurring during incubations at 37°C with the two isotypes. The findings are discussed with particular reference to observations on the modulation of mouse thymus leukaemia (TL) antigens. |
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| title_short |
The Influence of Antigen Density and a Comparison of IgG and IgM Antibodies in the Anti-Complementary Modulation of Lymphocytic Surface Immunoglobulin |
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ANDERSON, V. A. ROBINSON, D. S. F. STEVENSON, G. T. |
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