Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes
Summary Background Both intercellular and intracellular signals are transduced primarily by interactions of secreted and/or membrane-anchored polypeptides, and they play a pivotal role in regulating proliferation, differentiation and apoptosis of keratinocytes within the epidermis. Despite recent id...
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| Autor*in: |
Bonkobara, M. [verfasserIn] Das, A. [verfasserIn] Takao, J. [verfasserIn] |
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E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Science Ltd ; 2003 |
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Online-Ressource |
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| Reproduktion: |
2003 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: British journal of dermatology - Oxford : Wiley-Blackwell, 1892, 148(2003), 4, Seite 0 |
| Übergeordnetes Werk: |
volume:148 ; year:2003 ; number:4 ; pages:0 |
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| DOI / URN: |
10.1046/j.1365-2133.2003.05244.x |
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NLEJ242119204 |
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| 520 | |a Summary Background Both intercellular and intracellular signals are transduced primarily by interactions of secreted and/or membrane-anchored polypeptides, and they play a pivotal role in regulating proliferation, differentiation and apoptosis of keratinocytes within the epidermis. Despite recent identification of these polypeptides, it is likely that several important molecules remain undisclosed. Objectives To identify novel genes encoding secreted or membrane-anchored polypeptides expressed by human keratinocytes. Methods We employed a signal sequence (SS) trap of a 5′-end-enriched cDNA library prepared from primary cultured human keratinocytes. Gene expression analysis was performed using Northern blotting. Results Screening of 4018 cDNA clones yielded 82 positive clones (57 independent genes), most of which encoded SSs in their N-termini. Most of the positive clones were known genes registered in the GenBank database. Seven genes were identified in the EST database, four of which encoded novel membrane-anchored polypeptides with features of type I transmembrane proteins; the other three genes encoded novel non-type I transmembrane polypeptides. These EST genes were expressed differentially by keratinocytes subjected to low vs. high calcium concentrations and by basal vs. squamous cell carcinomas. Conclusions Using the SS trap, we isolated many genes known to be involved in constituting epidermal structures and others that had not previously been associated with keratinocytes. In addition, we identified novel genes (EST genes) that differ in kinetics of gene expression in keratinocyte differentiation. Our results validate the effective use of this SS trap method for identifying secreted and membrane-anchored polypeptides expressed by human keratinocytes. The identification will better illuminate the molecular mechanisms responsible for co-ordinated regulation of epidermal homeostasis. | ||
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| 700 | 1 | |a Takao, J. |e verfasserin |4 aut | |
| 700 | 1 | |a Cruz, P.D. |4 oth | |
| 700 | 1 | |a Ariizumi, K. |4 oth | |
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10.1046/j.1365-2133.2003.05244.x doi (DE-627)NLEJ242119204 DE-627 ger DE-627 rakwb Bonkobara, M. verfasserin aut Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes Oxford, UK Blackwell Science Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Background Both intercellular and intracellular signals are transduced primarily by interactions of secreted and/or membrane-anchored polypeptides, and they play a pivotal role in regulating proliferation, differentiation and apoptosis of keratinocytes within the epidermis. Despite recent identification of these polypeptides, it is likely that several important molecules remain undisclosed. Objectives To identify novel genes encoding secreted or membrane-anchored polypeptides expressed by human keratinocytes. Methods We employed a signal sequence (SS) trap of a 5′-end-enriched cDNA library prepared from primary cultured human keratinocytes. Gene expression analysis was performed using Northern blotting. Results Screening of 4018 cDNA clones yielded 82 positive clones (57 independent genes), most of which encoded SSs in their N-termini. Most of the positive clones were known genes registered in the GenBank database. Seven genes were identified in the EST database, four of which encoded novel membrane-anchored polypeptides with features of type I transmembrane proteins; the other three genes encoded novel non-type I transmembrane polypeptides. These EST genes were expressed differentially by keratinocytes subjected to low vs. high calcium concentrations and by basal vs. squamous cell carcinomas. Conclusions Using the SS trap, we isolated many genes known to be involved in constituting epidermal structures and others that had not previously been associated with keratinocytes. In addition, we identified novel genes (EST genes) that differ in kinetics of gene expression in keratinocyte differentiation. Our results validate the effective use of this SS trap method for identifying secreted and membrane-anchored polypeptides expressed by human keratinocytes. The identification will better illuminate the molecular mechanisms responsible for co-ordinated regulation of epidermal homeostasis. 2003 Blackwell Publishing Journal Backfiles 1879-2005 |2003|||||||||| cDNA cloning Das, A. verfasserin aut Takao, J. verfasserin aut Cruz, P.D. oth Ariizumi, K. oth In British journal of dermatology Oxford : Wiley-Blackwell, 1892 148(2003), 4, Seite 0 Online-Ressource (DE-627)NLEJ24392786X (DE-600)2004086-6 1365-2133 nnns volume:148 year:2003 number:4 pages:0 http://dx.doi.org/10.1046/j.1365-2133.2003.05244.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 148 2003 4 0 |
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10.1046/j.1365-2133.2003.05244.x doi (DE-627)NLEJ242119204 DE-627 ger DE-627 rakwb Bonkobara, M. verfasserin aut Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes Oxford, UK Blackwell Science Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Background Both intercellular and intracellular signals are transduced primarily by interactions of secreted and/or membrane-anchored polypeptides, and they play a pivotal role in regulating proliferation, differentiation and apoptosis of keratinocytes within the epidermis. Despite recent identification of these polypeptides, it is likely that several important molecules remain undisclosed. Objectives To identify novel genes encoding secreted or membrane-anchored polypeptides expressed by human keratinocytes. Methods We employed a signal sequence (SS) trap of a 5′-end-enriched cDNA library prepared from primary cultured human keratinocytes. Gene expression analysis was performed using Northern blotting. Results Screening of 4018 cDNA clones yielded 82 positive clones (57 independent genes), most of which encoded SSs in their N-termini. Most of the positive clones were known genes registered in the GenBank database. Seven genes were identified in the EST database, four of which encoded novel membrane-anchored polypeptides with features of type I transmembrane proteins; the other three genes encoded novel non-type I transmembrane polypeptides. These EST genes were expressed differentially by keratinocytes subjected to low vs. high calcium concentrations and by basal vs. squamous cell carcinomas. Conclusions Using the SS trap, we isolated many genes known to be involved in constituting epidermal structures and others that had not previously been associated with keratinocytes. In addition, we identified novel genes (EST genes) that differ in kinetics of gene expression in keratinocyte differentiation. Our results validate the effective use of this SS trap method for identifying secreted and membrane-anchored polypeptides expressed by human keratinocytes. The identification will better illuminate the molecular mechanisms responsible for co-ordinated regulation of epidermal homeostasis. 2003 Blackwell Publishing Journal Backfiles 1879-2005 |2003|||||||||| cDNA cloning Das, A. verfasserin aut Takao, J. verfasserin aut Cruz, P.D. oth Ariizumi, K. oth In British journal of dermatology Oxford : Wiley-Blackwell, 1892 148(2003), 4, Seite 0 Online-Ressource (DE-627)NLEJ24392786X (DE-600)2004086-6 1365-2133 nnns volume:148 year:2003 number:4 pages:0 http://dx.doi.org/10.1046/j.1365-2133.2003.05244.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 148 2003 4 0 |
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10.1046/j.1365-2133.2003.05244.x doi (DE-627)NLEJ242119204 DE-627 ger DE-627 rakwb Bonkobara, M. verfasserin aut Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes Oxford, UK Blackwell Science Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Background Both intercellular and intracellular signals are transduced primarily by interactions of secreted and/or membrane-anchored polypeptides, and they play a pivotal role in regulating proliferation, differentiation and apoptosis of keratinocytes within the epidermis. Despite recent identification of these polypeptides, it is likely that several important molecules remain undisclosed. Objectives To identify novel genes encoding secreted or membrane-anchored polypeptides expressed by human keratinocytes. Methods We employed a signal sequence (SS) trap of a 5′-end-enriched cDNA library prepared from primary cultured human keratinocytes. Gene expression analysis was performed using Northern blotting. Results Screening of 4018 cDNA clones yielded 82 positive clones (57 independent genes), most of which encoded SSs in their N-termini. Most of the positive clones were known genes registered in the GenBank database. Seven genes were identified in the EST database, four of which encoded novel membrane-anchored polypeptides with features of type I transmembrane proteins; the other three genes encoded novel non-type I transmembrane polypeptides. These EST genes were expressed differentially by keratinocytes subjected to low vs. high calcium concentrations and by basal vs. squamous cell carcinomas. Conclusions Using the SS trap, we isolated many genes known to be involved in constituting epidermal structures and others that had not previously been associated with keratinocytes. In addition, we identified novel genes (EST genes) that differ in kinetics of gene expression in keratinocyte differentiation. Our results validate the effective use of this SS trap method for identifying secreted and membrane-anchored polypeptides expressed by human keratinocytes. The identification will better illuminate the molecular mechanisms responsible for co-ordinated regulation of epidermal homeostasis. 2003 Blackwell Publishing Journal Backfiles 1879-2005 |2003|||||||||| cDNA cloning Das, A. verfasserin aut Takao, J. verfasserin aut Cruz, P.D. oth Ariizumi, K. oth In British journal of dermatology Oxford : Wiley-Blackwell, 1892 148(2003), 4, Seite 0 Online-Ressource (DE-627)NLEJ24392786X (DE-600)2004086-6 1365-2133 nnns volume:148 year:2003 number:4 pages:0 http://dx.doi.org/10.1046/j.1365-2133.2003.05244.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 148 2003 4 0 |
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10.1046/j.1365-2133.2003.05244.x doi (DE-627)NLEJ242119204 DE-627 ger DE-627 rakwb Bonkobara, M. verfasserin aut Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes Oxford, UK Blackwell Science Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Background Both intercellular and intracellular signals are transduced primarily by interactions of secreted and/or membrane-anchored polypeptides, and they play a pivotal role in regulating proliferation, differentiation and apoptosis of keratinocytes within the epidermis. Despite recent identification of these polypeptides, it is likely that several important molecules remain undisclosed. Objectives To identify novel genes encoding secreted or membrane-anchored polypeptides expressed by human keratinocytes. Methods We employed a signal sequence (SS) trap of a 5′-end-enriched cDNA library prepared from primary cultured human keratinocytes. Gene expression analysis was performed using Northern blotting. Results Screening of 4018 cDNA clones yielded 82 positive clones (57 independent genes), most of which encoded SSs in their N-termini. Most of the positive clones were known genes registered in the GenBank database. Seven genes were identified in the EST database, four of which encoded novel membrane-anchored polypeptides with features of type I transmembrane proteins; the other three genes encoded novel non-type I transmembrane polypeptides. These EST genes were expressed differentially by keratinocytes subjected to low vs. high calcium concentrations and by basal vs. squamous cell carcinomas. Conclusions Using the SS trap, we isolated many genes known to be involved in constituting epidermal structures and others that had not previously been associated with keratinocytes. In addition, we identified novel genes (EST genes) that differ in kinetics of gene expression in keratinocyte differentiation. Our results validate the effective use of this SS trap method for identifying secreted and membrane-anchored polypeptides expressed by human keratinocytes. The identification will better illuminate the molecular mechanisms responsible for co-ordinated regulation of epidermal homeostasis. 2003 Blackwell Publishing Journal Backfiles 1879-2005 |2003|||||||||| cDNA cloning Das, A. verfasserin aut Takao, J. verfasserin aut Cruz, P.D. oth Ariizumi, K. oth In British journal of dermatology Oxford : Wiley-Blackwell, 1892 148(2003), 4, Seite 0 Online-Ressource (DE-627)NLEJ24392786X (DE-600)2004086-6 1365-2133 nnns volume:148 year:2003 number:4 pages:0 http://dx.doi.org/10.1046/j.1365-2133.2003.05244.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 148 2003 4 0 |
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10.1046/j.1365-2133.2003.05244.x doi (DE-627)NLEJ242119204 DE-627 ger DE-627 rakwb Bonkobara, M. verfasserin aut Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes Oxford, UK Blackwell Science Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Summary Background Both intercellular and intracellular signals are transduced primarily by interactions of secreted and/or membrane-anchored polypeptides, and they play a pivotal role in regulating proliferation, differentiation and apoptosis of keratinocytes within the epidermis. Despite recent identification of these polypeptides, it is likely that several important molecules remain undisclosed. Objectives To identify novel genes encoding secreted or membrane-anchored polypeptides expressed by human keratinocytes. Methods We employed a signal sequence (SS) trap of a 5′-end-enriched cDNA library prepared from primary cultured human keratinocytes. Gene expression analysis was performed using Northern blotting. Results Screening of 4018 cDNA clones yielded 82 positive clones (57 independent genes), most of which encoded SSs in their N-termini. Most of the positive clones were known genes registered in the GenBank database. Seven genes were identified in the EST database, four of which encoded novel membrane-anchored polypeptides with features of type I transmembrane proteins; the other three genes encoded novel non-type I transmembrane polypeptides. These EST genes were expressed differentially by keratinocytes subjected to low vs. high calcium concentrations and by basal vs. squamous cell carcinomas. Conclusions Using the SS trap, we isolated many genes known to be involved in constituting epidermal structures and others that had not previously been associated with keratinocytes. In addition, we identified novel genes (EST genes) that differ in kinetics of gene expression in keratinocyte differentiation. Our results validate the effective use of this SS trap method for identifying secreted and membrane-anchored polypeptides expressed by human keratinocytes. The identification will better illuminate the molecular mechanisms responsible for co-ordinated regulation of epidermal homeostasis. 2003 Blackwell Publishing Journal Backfiles 1879-2005 |2003|||||||||| cDNA cloning Das, A. verfasserin aut Takao, J. verfasserin aut Cruz, P.D. oth Ariizumi, K. oth In British journal of dermatology Oxford : Wiley-Blackwell, 1892 148(2003), 4, Seite 0 Online-Ressource (DE-627)NLEJ24392786X (DE-600)2004086-6 1365-2133 nnns volume:148 year:2003 number:4 pages:0 http://dx.doi.org/10.1046/j.1365-2133.2003.05244.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 148 2003 4 0 |
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Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes |
| abstract |
Summary Background Both intercellular and intracellular signals are transduced primarily by interactions of secreted and/or membrane-anchored polypeptides, and they play a pivotal role in regulating proliferation, differentiation and apoptosis of keratinocytes within the epidermis. Despite recent identification of these polypeptides, it is likely that several important molecules remain undisclosed. Objectives To identify novel genes encoding secreted or membrane-anchored polypeptides expressed by human keratinocytes. Methods We employed a signal sequence (SS) trap of a 5′-end-enriched cDNA library prepared from primary cultured human keratinocytes. Gene expression analysis was performed using Northern blotting. Results Screening of 4018 cDNA clones yielded 82 positive clones (57 independent genes), most of which encoded SSs in their N-termini. Most of the positive clones were known genes registered in the GenBank database. Seven genes were identified in the EST database, four of which encoded novel membrane-anchored polypeptides with features of type I transmembrane proteins; the other three genes encoded novel non-type I transmembrane polypeptides. These EST genes were expressed differentially by keratinocytes subjected to low vs. high calcium concentrations and by basal vs. squamous cell carcinomas. Conclusions Using the SS trap, we isolated many genes known to be involved in constituting epidermal structures and others that had not previously been associated with keratinocytes. In addition, we identified novel genes (EST genes) that differ in kinetics of gene expression in keratinocyte differentiation. Our results validate the effective use of this SS trap method for identifying secreted and membrane-anchored polypeptides expressed by human keratinocytes. The identification will better illuminate the molecular mechanisms responsible for co-ordinated regulation of epidermal homeostasis. |
| abstractGer |
Summary Background Both intercellular and intracellular signals are transduced primarily by interactions of secreted and/or membrane-anchored polypeptides, and they play a pivotal role in regulating proliferation, differentiation and apoptosis of keratinocytes within the epidermis. Despite recent identification of these polypeptides, it is likely that several important molecules remain undisclosed. Objectives To identify novel genes encoding secreted or membrane-anchored polypeptides expressed by human keratinocytes. Methods We employed a signal sequence (SS) trap of a 5′-end-enriched cDNA library prepared from primary cultured human keratinocytes. Gene expression analysis was performed using Northern blotting. Results Screening of 4018 cDNA clones yielded 82 positive clones (57 independent genes), most of which encoded SSs in their N-termini. Most of the positive clones were known genes registered in the GenBank database. Seven genes were identified in the EST database, four of which encoded novel membrane-anchored polypeptides with features of type I transmembrane proteins; the other three genes encoded novel non-type I transmembrane polypeptides. These EST genes were expressed differentially by keratinocytes subjected to low vs. high calcium concentrations and by basal vs. squamous cell carcinomas. Conclusions Using the SS trap, we isolated many genes known to be involved in constituting epidermal structures and others that had not previously been associated with keratinocytes. In addition, we identified novel genes (EST genes) that differ in kinetics of gene expression in keratinocyte differentiation. Our results validate the effective use of this SS trap method for identifying secreted and membrane-anchored polypeptides expressed by human keratinocytes. The identification will better illuminate the molecular mechanisms responsible for co-ordinated regulation of epidermal homeostasis. |
| abstract_unstemmed |
Summary Background Both intercellular and intracellular signals are transduced primarily by interactions of secreted and/or membrane-anchored polypeptides, and they play a pivotal role in regulating proliferation, differentiation and apoptosis of keratinocytes within the epidermis. Despite recent identification of these polypeptides, it is likely that several important molecules remain undisclosed. Objectives To identify novel genes encoding secreted or membrane-anchored polypeptides expressed by human keratinocytes. Methods We employed a signal sequence (SS) trap of a 5′-end-enriched cDNA library prepared from primary cultured human keratinocytes. Gene expression analysis was performed using Northern blotting. Results Screening of 4018 cDNA clones yielded 82 positive clones (57 independent genes), most of which encoded SSs in their N-termini. Most of the positive clones were known genes registered in the GenBank database. Seven genes were identified in the EST database, four of which encoded novel membrane-anchored polypeptides with features of type I transmembrane proteins; the other three genes encoded novel non-type I transmembrane polypeptides. These EST genes were expressed differentially by keratinocytes subjected to low vs. high calcium concentrations and by basal vs. squamous cell carcinomas. Conclusions Using the SS trap, we isolated many genes known to be involved in constituting epidermal structures and others that had not previously been associated with keratinocytes. In addition, we identified novel genes (EST genes) that differ in kinetics of gene expression in keratinocyte differentiation. Our results validate the effective use of this SS trap method for identifying secreted and membrane-anchored polypeptides expressed by human keratinocytes. The identification will better illuminate the molecular mechanisms responsible for co-ordinated regulation of epidermal homeostasis. |
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| title_short |
Identification of novel genes for secreted and membrane-anchored proteins in human keratinocytes |
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http://dx.doi.org/10.1046/j.1365-2133.2003.05244.x |
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| author2 |
Das, A. Takao, J. Cruz, P.D. Ariizumi, K. |
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Das, A. Takao, J. Cruz, P.D. Ariizumi, K. |
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10.1046/j.1365-2133.2003.05244.x |
| up_date |
2024-07-06T00:54:04.946Z |
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