Cell migration and MMP-9 secretion are increased by epidermal growth factor in HaCa T-ras transfected cells
Abstract: Mutated RAS oncoproteins and epidermal growth factor (EGF) are thought to contribute to the proliferative, invasive and metastatic properties of transformed cells. In the present study, we investigated the role of EGF in two H-ras transfected clones and compared it to that in the parental...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Charvat, Sandrine [verfasserIn] Chignol, Marie-Christine [verfasserIn] Souchier, Catherine [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1998 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2007 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Experimental dermatology - Oxford : Wiley-Blackwell, 1992, 7(1998), 4, Seite 0 |
| Übergeordnetes Werk: |
volume:7 ; year:1998 ; number:4 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1111/j.1600-0625.1998.tb00322.x |
|---|
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NLEJ242378749 |
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| 520 | |a Abstract: Mutated RAS oncoproteins and epidermal growth factor (EGF) are thought to contribute to the proliferative, invasive and metastatic properties of transformed cells. In the present study, we investigated the role of EGF in two H-ras transfected clones and compared it to that in the parental cell line, HaCaT and primary cultured keratinocytes. Our findings show that the motility on type I collagen, measured by the migration index, was similar for both the HaCaT cell line and normal human keratinocytes, whereas it was higher for the HaCaT-ras clones. These results suggest an involvement of the ras oncogene in the stimulation of cell migration. EGF, in cell pretreatment or during the migration assay also caused an increase in migration of all the cells, but preserved the difference between HaCaT and HaCaT-ras. However, no significant difference in EGF-R expression was detected between normal cultured keratinocytes, HaCaT and HaCaT-ras cell lines with or without EGF pretreatment. Moreover, when the cells were stimulated with EGF, the MMP-9 activity was greatly increased in a dose-dependent manner in all the cells, and EGF stimulation particularly highlights the increased amount of MMP-9 in HaCaT-ras cells compared to HaCaT cells. In conclusion, EGF is able to enhance motility and to up-regulate MMP-9 activity in all cells, but with a higher impact in HaCaT-ras cells without an overexpression of EGF-R. As EGF acts in synergy with the H-ras mutation, they could be implicated in the local invasion by the HaCaT-ras clones. | ||
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| 700 | 1 | |a Schmitt, Daniel |4 oth | |
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10.1111/j.1600-0625.1998.tb00322.x doi (DE-627)NLEJ242378749 DE-627 ger DE-627 rakwb Charvat, Sandrine verfasserin aut Cell migration and MMP-9 secretion are increased by epidermal growth factor in HaCa T-ras transfected cells Oxford, UK Blackwell Publishing Ltd 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Mutated RAS oncoproteins and epidermal growth factor (EGF) are thought to contribute to the proliferative, invasive and metastatic properties of transformed cells. In the present study, we investigated the role of EGF in two H-ras transfected clones and compared it to that in the parental cell line, HaCaT and primary cultured keratinocytes. Our findings show that the motility on type I collagen, measured by the migration index, was similar for both the HaCaT cell line and normal human keratinocytes, whereas it was higher for the HaCaT-ras clones. These results suggest an involvement of the ras oncogene in the stimulation of cell migration. EGF, in cell pretreatment or during the migration assay also caused an increase in migration of all the cells, but preserved the difference between HaCaT and HaCaT-ras. However, no significant difference in EGF-R expression was detected between normal cultured keratinocytes, HaCaT and HaCaT-ras cell lines with or without EGF pretreatment. Moreover, when the cells were stimulated with EGF, the MMP-9 activity was greatly increased in a dose-dependent manner in all the cells, and EGF stimulation particularly highlights the increased amount of MMP-9 in HaCaT-ras cells compared to HaCaT cells. In conclusion, EGF is able to enhance motility and to up-regulate MMP-9 activity in all cells, but with a higher impact in HaCaT-ras cells without an overexpression of EGF-R. As EGF acts in synergy with the H-ras mutation, they could be implicated in the local invasion by the HaCaT-ras clones. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| keratinocytes Chignol, Marie-Christine verfasserin aut Souchier, Catherine verfasserin aut Griel, Caroline Le oth Schmitt, Daniel oth Serres, Mireille oth In Experimental dermatology Oxford : Wiley-Blackwell, 1992 7(1998), 4, Seite 0 Online-Ressource (DE-627)NLEJ243926081 (DE-600)2026228-0 1600-0625 nnns volume:7 year:1998 number:4 pages:0 http://dx.doi.org/10.1111/j.1600-0625.1998.tb00322.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 7 1998 4 0 |
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10.1111/j.1600-0625.1998.tb00322.x doi (DE-627)NLEJ242378749 DE-627 ger DE-627 rakwb Charvat, Sandrine verfasserin aut Cell migration and MMP-9 secretion are increased by epidermal growth factor in HaCa T-ras transfected cells Oxford, UK Blackwell Publishing Ltd 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Mutated RAS oncoproteins and epidermal growth factor (EGF) are thought to contribute to the proliferative, invasive and metastatic properties of transformed cells. In the present study, we investigated the role of EGF in two H-ras transfected clones and compared it to that in the parental cell line, HaCaT and primary cultured keratinocytes. Our findings show that the motility on type I collagen, measured by the migration index, was similar for both the HaCaT cell line and normal human keratinocytes, whereas it was higher for the HaCaT-ras clones. These results suggest an involvement of the ras oncogene in the stimulation of cell migration. EGF, in cell pretreatment or during the migration assay also caused an increase in migration of all the cells, but preserved the difference between HaCaT and HaCaT-ras. However, no significant difference in EGF-R expression was detected between normal cultured keratinocytes, HaCaT and HaCaT-ras cell lines with or without EGF pretreatment. Moreover, when the cells were stimulated with EGF, the MMP-9 activity was greatly increased in a dose-dependent manner in all the cells, and EGF stimulation particularly highlights the increased amount of MMP-9 in HaCaT-ras cells compared to HaCaT cells. In conclusion, EGF is able to enhance motility and to up-regulate MMP-9 activity in all cells, but with a higher impact in HaCaT-ras cells without an overexpression of EGF-R. As EGF acts in synergy with the H-ras mutation, they could be implicated in the local invasion by the HaCaT-ras clones. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| keratinocytes Chignol, Marie-Christine verfasserin aut Souchier, Catherine verfasserin aut Griel, Caroline Le oth Schmitt, Daniel oth Serres, Mireille oth In Experimental dermatology Oxford : Wiley-Blackwell, 1992 7(1998), 4, Seite 0 Online-Ressource (DE-627)NLEJ243926081 (DE-600)2026228-0 1600-0625 nnns volume:7 year:1998 number:4 pages:0 http://dx.doi.org/10.1111/j.1600-0625.1998.tb00322.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 7 1998 4 0 |
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10.1111/j.1600-0625.1998.tb00322.x doi (DE-627)NLEJ242378749 DE-627 ger DE-627 rakwb Charvat, Sandrine verfasserin aut Cell migration and MMP-9 secretion are increased by epidermal growth factor in HaCa T-ras transfected cells Oxford, UK Blackwell Publishing Ltd 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Mutated RAS oncoproteins and epidermal growth factor (EGF) are thought to contribute to the proliferative, invasive and metastatic properties of transformed cells. In the present study, we investigated the role of EGF in two H-ras transfected clones and compared it to that in the parental cell line, HaCaT and primary cultured keratinocytes. Our findings show that the motility on type I collagen, measured by the migration index, was similar for both the HaCaT cell line and normal human keratinocytes, whereas it was higher for the HaCaT-ras clones. These results suggest an involvement of the ras oncogene in the stimulation of cell migration. EGF, in cell pretreatment or during the migration assay also caused an increase in migration of all the cells, but preserved the difference between HaCaT and HaCaT-ras. However, no significant difference in EGF-R expression was detected between normal cultured keratinocytes, HaCaT and HaCaT-ras cell lines with or without EGF pretreatment. Moreover, when the cells were stimulated with EGF, the MMP-9 activity was greatly increased in a dose-dependent manner in all the cells, and EGF stimulation particularly highlights the increased amount of MMP-9 in HaCaT-ras cells compared to HaCaT cells. In conclusion, EGF is able to enhance motility and to up-regulate MMP-9 activity in all cells, but with a higher impact in HaCaT-ras cells without an overexpression of EGF-R. As EGF acts in synergy with the H-ras mutation, they could be implicated in the local invasion by the HaCaT-ras clones. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| keratinocytes Chignol, Marie-Christine verfasserin aut Souchier, Catherine verfasserin aut Griel, Caroline Le oth Schmitt, Daniel oth Serres, Mireille oth In Experimental dermatology Oxford : Wiley-Blackwell, 1992 7(1998), 4, Seite 0 Online-Ressource (DE-627)NLEJ243926081 (DE-600)2026228-0 1600-0625 nnns volume:7 year:1998 number:4 pages:0 http://dx.doi.org/10.1111/j.1600-0625.1998.tb00322.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 7 1998 4 0 |
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10.1111/j.1600-0625.1998.tb00322.x doi (DE-627)NLEJ242378749 DE-627 ger DE-627 rakwb Charvat, Sandrine verfasserin aut Cell migration and MMP-9 secretion are increased by epidermal growth factor in HaCa T-ras transfected cells Oxford, UK Blackwell Publishing Ltd 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Mutated RAS oncoproteins and epidermal growth factor (EGF) are thought to contribute to the proliferative, invasive and metastatic properties of transformed cells. In the present study, we investigated the role of EGF in two H-ras transfected clones and compared it to that in the parental cell line, HaCaT and primary cultured keratinocytes. Our findings show that the motility on type I collagen, measured by the migration index, was similar for both the HaCaT cell line and normal human keratinocytes, whereas it was higher for the HaCaT-ras clones. These results suggest an involvement of the ras oncogene in the stimulation of cell migration. EGF, in cell pretreatment or during the migration assay also caused an increase in migration of all the cells, but preserved the difference between HaCaT and HaCaT-ras. However, no significant difference in EGF-R expression was detected between normal cultured keratinocytes, HaCaT and HaCaT-ras cell lines with or without EGF pretreatment. Moreover, when the cells were stimulated with EGF, the MMP-9 activity was greatly increased in a dose-dependent manner in all the cells, and EGF stimulation particularly highlights the increased amount of MMP-9 in HaCaT-ras cells compared to HaCaT cells. In conclusion, EGF is able to enhance motility and to up-regulate MMP-9 activity in all cells, but with a higher impact in HaCaT-ras cells without an overexpression of EGF-R. As EGF acts in synergy with the H-ras mutation, they could be implicated in the local invasion by the HaCaT-ras clones. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| keratinocytes Chignol, Marie-Christine verfasserin aut Souchier, Catherine verfasserin aut Griel, Caroline Le oth Schmitt, Daniel oth Serres, Mireille oth In Experimental dermatology Oxford : Wiley-Blackwell, 1992 7(1998), 4, Seite 0 Online-Ressource (DE-627)NLEJ243926081 (DE-600)2026228-0 1600-0625 nnns volume:7 year:1998 number:4 pages:0 http://dx.doi.org/10.1111/j.1600-0625.1998.tb00322.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 7 1998 4 0 |
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10.1111/j.1600-0625.1998.tb00322.x doi (DE-627)NLEJ242378749 DE-627 ger DE-627 rakwb Charvat, Sandrine verfasserin aut Cell migration and MMP-9 secretion are increased by epidermal growth factor in HaCa T-ras transfected cells Oxford, UK Blackwell Publishing Ltd 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Mutated RAS oncoproteins and epidermal growth factor (EGF) are thought to contribute to the proliferative, invasive and metastatic properties of transformed cells. In the present study, we investigated the role of EGF in two H-ras transfected clones and compared it to that in the parental cell line, HaCaT and primary cultured keratinocytes. Our findings show that the motility on type I collagen, measured by the migration index, was similar for both the HaCaT cell line and normal human keratinocytes, whereas it was higher for the HaCaT-ras clones. These results suggest an involvement of the ras oncogene in the stimulation of cell migration. EGF, in cell pretreatment or during the migration assay also caused an increase in migration of all the cells, but preserved the difference between HaCaT and HaCaT-ras. However, no significant difference in EGF-R expression was detected between normal cultured keratinocytes, HaCaT and HaCaT-ras cell lines with or without EGF pretreatment. Moreover, when the cells were stimulated with EGF, the MMP-9 activity was greatly increased in a dose-dependent manner in all the cells, and EGF stimulation particularly highlights the increased amount of MMP-9 in HaCaT-ras cells compared to HaCaT cells. In conclusion, EGF is able to enhance motility and to up-regulate MMP-9 activity in all cells, but with a higher impact in HaCaT-ras cells without an overexpression of EGF-R. As EGF acts in synergy with the H-ras mutation, they could be implicated in the local invasion by the HaCaT-ras clones. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| keratinocytes Chignol, Marie-Christine verfasserin aut Souchier, Catherine verfasserin aut Griel, Caroline Le oth Schmitt, Daniel oth Serres, Mireille oth In Experimental dermatology Oxford : Wiley-Blackwell, 1992 7(1998), 4, Seite 0 Online-Ressource (DE-627)NLEJ243926081 (DE-600)2026228-0 1600-0625 nnns volume:7 year:1998 number:4 pages:0 http://dx.doi.org/10.1111/j.1600-0625.1998.tb00322.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 7 1998 4 0 |
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Cell migration and MMP-9 secretion are increased by epidermal growth factor in HaCa T-ras transfected cells |
| abstract |
Abstract: Mutated RAS oncoproteins and epidermal growth factor (EGF) are thought to contribute to the proliferative, invasive and metastatic properties of transformed cells. In the present study, we investigated the role of EGF in two H-ras transfected clones and compared it to that in the parental cell line, HaCaT and primary cultured keratinocytes. Our findings show that the motility on type I collagen, measured by the migration index, was similar for both the HaCaT cell line and normal human keratinocytes, whereas it was higher for the HaCaT-ras clones. These results suggest an involvement of the ras oncogene in the stimulation of cell migration. EGF, in cell pretreatment or during the migration assay also caused an increase in migration of all the cells, but preserved the difference between HaCaT and HaCaT-ras. However, no significant difference in EGF-R expression was detected between normal cultured keratinocytes, HaCaT and HaCaT-ras cell lines with or without EGF pretreatment. Moreover, when the cells were stimulated with EGF, the MMP-9 activity was greatly increased in a dose-dependent manner in all the cells, and EGF stimulation particularly highlights the increased amount of MMP-9 in HaCaT-ras cells compared to HaCaT cells. In conclusion, EGF is able to enhance motility and to up-regulate MMP-9 activity in all cells, but with a higher impact in HaCaT-ras cells without an overexpression of EGF-R. As EGF acts in synergy with the H-ras mutation, they could be implicated in the local invasion by the HaCaT-ras clones. |
| abstractGer |
Abstract: Mutated RAS oncoproteins and epidermal growth factor (EGF) are thought to contribute to the proliferative, invasive and metastatic properties of transformed cells. In the present study, we investigated the role of EGF in two H-ras transfected clones and compared it to that in the parental cell line, HaCaT and primary cultured keratinocytes. Our findings show that the motility on type I collagen, measured by the migration index, was similar for both the HaCaT cell line and normal human keratinocytes, whereas it was higher for the HaCaT-ras clones. These results suggest an involvement of the ras oncogene in the stimulation of cell migration. EGF, in cell pretreatment or during the migration assay also caused an increase in migration of all the cells, but preserved the difference between HaCaT and HaCaT-ras. However, no significant difference in EGF-R expression was detected between normal cultured keratinocytes, HaCaT and HaCaT-ras cell lines with or without EGF pretreatment. Moreover, when the cells were stimulated with EGF, the MMP-9 activity was greatly increased in a dose-dependent manner in all the cells, and EGF stimulation particularly highlights the increased amount of MMP-9 in HaCaT-ras cells compared to HaCaT cells. In conclusion, EGF is able to enhance motility and to up-regulate MMP-9 activity in all cells, but with a higher impact in HaCaT-ras cells without an overexpression of EGF-R. As EGF acts in synergy with the H-ras mutation, they could be implicated in the local invasion by the HaCaT-ras clones. |
| abstract_unstemmed |
Abstract: Mutated RAS oncoproteins and epidermal growth factor (EGF) are thought to contribute to the proliferative, invasive and metastatic properties of transformed cells. In the present study, we investigated the role of EGF in two H-ras transfected clones and compared it to that in the parental cell line, HaCaT and primary cultured keratinocytes. Our findings show that the motility on type I collagen, measured by the migration index, was similar for both the HaCaT cell line and normal human keratinocytes, whereas it was higher for the HaCaT-ras clones. These results suggest an involvement of the ras oncogene in the stimulation of cell migration. EGF, in cell pretreatment or during the migration assay also caused an increase in migration of all the cells, but preserved the difference between HaCaT and HaCaT-ras. However, no significant difference in EGF-R expression was detected between normal cultured keratinocytes, HaCaT and HaCaT-ras cell lines with or without EGF pretreatment. Moreover, when the cells were stimulated with EGF, the MMP-9 activity was greatly increased in a dose-dependent manner in all the cells, and EGF stimulation particularly highlights the increased amount of MMP-9 in HaCaT-ras cells compared to HaCaT cells. In conclusion, EGF is able to enhance motility and to up-regulate MMP-9 activity in all cells, but with a higher impact in HaCaT-ras cells without an overexpression of EGF-R. As EGF acts in synergy with the H-ras mutation, they could be implicated in the local invasion by the HaCaT-ras clones. |
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| title_short |
Cell migration and MMP-9 secretion are increased by epidermal growth factor in HaCa T-ras transfected cells |
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http://dx.doi.org/10.1111/j.1600-0625.1998.tb00322.x |
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| author2 |
Chignol, Marie-Christine Souchier, Catherine Griel, Caroline Le Schmitt, Daniel Serres, Mireille |
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Chignol, Marie-Christine Souchier, Catherine Griel, Caroline Le Schmitt, Daniel Serres, Mireille |
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10.1111/j.1600-0625.1998.tb00322.x |
| up_date |
2024-07-06T01:46:26.421Z |
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