Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction
Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RN...
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Oxford, UK: Blackwell Publishing Ltd ; 1999 |
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Online-Ressource |
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2008 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: Forest pathology - Oxford [u.a.] : Wiley-Blackwell, 1999, 29(1999), 3, Seite 0 |
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volume:29 ; year:1999 ; number:3 ; pages:0 |
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| DOI / URN: |
10.1046/j.1439-0329.1999.00141.x |
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| 245 | 1 | 0 | |a Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction |
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| 520 | |a Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (P. cambivora), 4pg (P. quercina), and 2pg (P. citricola) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina, and P. cambivora in seedlings of pendunculate oak (Quercus robur) and European beech (Fagus sylvatica) which were artificially infected under controlled conditions. | ||
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| 700 | 1 | |a Duncan, J. M. |4 oth | |
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| 700 | 1 | |a Langebartels, C. |4 oth | |
| 700 | 1 | |a Jr, H. Sandermann |4 oth | |
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10.1046/j.1439-0329.1999.00141.x doi (DE-627)NLEJ242452140 DE-627 ger DE-627 rakwb Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction Oxford, UK Blackwell Publishing Ltd 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (P. cambivora), 4pg (P. quercina), and 2pg (P. citricola) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina, and P. cambivora in seedlings of pendunculate oak (Quercus robur) and European beech (Fagus sylvatica) which were artificially infected under controlled conditions. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Schubert, R. oth Bahnweg, G. oth Nechwatal, J. oth Jung, T. oth Cooke, D. E. L. oth Duncan, J. M. oth Müller-Starck, G. oth Langebartels, C. oth Jr, H. Sandermann oth Oßwald, W. oth In Forest pathology Oxford [u.a.] : Wiley-Blackwell, 1999 29(1999), 3, Seite 0 Online-Ressource (DE-627)NLEJ243925603 (DE-600)2020304-4 1439-0329 nnns volume:29 year:1999 number:3 pages:0 http://dx.doi.org/10.1046/j.1439-0329.1999.00141.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 1999 3 0 |
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10.1046/j.1439-0329.1999.00141.x doi (DE-627)NLEJ242452140 DE-627 ger DE-627 rakwb Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction Oxford, UK Blackwell Publishing Ltd 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (P. cambivora), 4pg (P. quercina), and 2pg (P. citricola) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina, and P. cambivora in seedlings of pendunculate oak (Quercus robur) and European beech (Fagus sylvatica) which were artificially infected under controlled conditions. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Schubert, R. oth Bahnweg, G. oth Nechwatal, J. oth Jung, T. oth Cooke, D. E. L. oth Duncan, J. M. oth Müller-Starck, G. oth Langebartels, C. oth Jr, H. Sandermann oth Oßwald, W. oth In Forest pathology Oxford [u.a.] : Wiley-Blackwell, 1999 29(1999), 3, Seite 0 Online-Ressource (DE-627)NLEJ243925603 (DE-600)2020304-4 1439-0329 nnns volume:29 year:1999 number:3 pages:0 http://dx.doi.org/10.1046/j.1439-0329.1999.00141.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 1999 3 0 |
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10.1046/j.1439-0329.1999.00141.x doi (DE-627)NLEJ242452140 DE-627 ger DE-627 rakwb Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction Oxford, UK Blackwell Publishing Ltd 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (P. cambivora), 4pg (P. quercina), and 2pg (P. citricola) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina, and P. cambivora in seedlings of pendunculate oak (Quercus robur) and European beech (Fagus sylvatica) which were artificially infected under controlled conditions. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Schubert, R. oth Bahnweg, G. oth Nechwatal, J. oth Jung, T. oth Cooke, D. E. L. oth Duncan, J. M. oth Müller-Starck, G. oth Langebartels, C. oth Jr, H. Sandermann oth Oßwald, W. oth In Forest pathology Oxford [u.a.] : Wiley-Blackwell, 1999 29(1999), 3, Seite 0 Online-Ressource (DE-627)NLEJ243925603 (DE-600)2020304-4 1439-0329 nnns volume:29 year:1999 number:3 pages:0 http://dx.doi.org/10.1046/j.1439-0329.1999.00141.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 1999 3 0 |
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10.1046/j.1439-0329.1999.00141.x doi (DE-627)NLEJ242452140 DE-627 ger DE-627 rakwb Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction Oxford, UK Blackwell Publishing Ltd 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (P. cambivora), 4pg (P. quercina), and 2pg (P. citricola) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina, and P. cambivora in seedlings of pendunculate oak (Quercus robur) and European beech (Fagus sylvatica) which were artificially infected under controlled conditions. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Schubert, R. oth Bahnweg, G. oth Nechwatal, J. oth Jung, T. oth Cooke, D. E. L. oth Duncan, J. M. oth Müller-Starck, G. oth Langebartels, C. oth Jr, H. Sandermann oth Oßwald, W. oth In Forest pathology Oxford [u.a.] : Wiley-Blackwell, 1999 29(1999), 3, Seite 0 Online-Ressource (DE-627)NLEJ243925603 (DE-600)2020304-4 1439-0329 nnns volume:29 year:1999 number:3 pages:0 http://dx.doi.org/10.1046/j.1439-0329.1999.00141.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 1999 3 0 |
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10.1046/j.1439-0329.1999.00141.x doi (DE-627)NLEJ242452140 DE-627 ger DE-627 rakwb Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction Oxford, UK Blackwell Publishing Ltd 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (P. cambivora), 4pg (P. quercina), and 2pg (P. citricola) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina, and P. cambivora in seedlings of pendunculate oak (Quercus robur) and European beech (Fagus sylvatica) which were artificially infected under controlled conditions. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Schubert, R. oth Bahnweg, G. oth Nechwatal, J. oth Jung, T. oth Cooke, D. E. L. oth Duncan, J. M. oth Müller-Starck, G. oth Langebartels, C. oth Jr, H. Sandermann oth Oßwald, W. oth In Forest pathology Oxford [u.a.] : Wiley-Blackwell, 1999 29(1999), 3, Seite 0 Online-Ressource (DE-627)NLEJ243925603 (DE-600)2020304-4 1439-0329 nnns volume:29 year:1999 number:3 pages:0 http://dx.doi.org/10.1046/j.1439-0329.1999.00141.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 1999 3 0 |
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detection and quantification of phytophthora species which are associated with root-rot diseases in european deciduous forests by species-specific polymerase chain reaction |
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Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction |
| abstract |
Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (P. cambivora), 4pg (P. quercina), and 2pg (P. citricola) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina, and P. cambivora in seedlings of pendunculate oak (Quercus robur) and European beech (Fagus sylvatica) which were artificially infected under controlled conditions. |
| abstractGer |
Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (P. cambivora), 4pg (P. quercina), and 2pg (P. citricola) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina, and P. cambivora in seedlings of pendunculate oak (Quercus robur) and European beech (Fagus sylvatica) which were artificially infected under controlled conditions. |
| abstract_unstemmed |
Oligonucleotide primers were developed for the polymerase chain reaction (PCR)-based detection of selected Phytophthora species which are known to cause root-rot diseases in European forest trees. The primer pair CITR1/CITR2, complementing both internal transcribed spacer regions of the ribosomal RNA genes, gave a 711 bp amplicon with Phytophthora citricola. The Phytophthora cambivora specific primer pair CAMB3/CAMB4, producing a 1105bp amplicon, as well as the Phytophthora quercina specific primer pair QUERC1/QUERC2, producing a 842 bp amplicon, were derived from randomly amplified polymorphic DNA (RAPD)-fragments presented in this paper. All three primer pairs revealed no undesirable cross-reaction with a diverse test collection of isolates including other Phytophthora species, Pythium, Xerocomus, Hebeloma, Russula, and Armillaria. Under the PCR conditions described the detection of a well discernable amplicon was possible down to 100 pg (P. cambivora), 4pg (P. quercina), and 2pg (P. citricola) target DNA. This diagnostic PCR system was able to detect P. citricola, P. quercina, and P. cambivora in seedlings of pendunculate oak (Quercus robur) and European beech (Fagus sylvatica) which were artificially infected under controlled conditions. |
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| title_short |
Detection and quantification of Phytophthora species which are associated with root-rot diseases in European deciduous forests by species-specific polymerase chain reaction |
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http://dx.doi.org/10.1046/j.1439-0329.1999.00141.x |
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Schubert, R. Bahnweg, G. Nechwatal, J. Jung, T. Cooke, D. E. L. Duncan, J. M. Müller-Starck, G. Langebartels, C. Jr, H. Sandermann Oßwald, W. |
| author2Str |
Schubert, R. Bahnweg, G. Nechwatal, J. Jung, T. Cooke, D. E. L. Duncan, J. M. Müller-Starck, G. Langebartels, C. Jr, H. Sandermann Oßwald, W. |
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| doi_str |
10.1046/j.1439-0329.1999.00141.x |
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2024-07-06T02:04:07.778Z |
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