Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo]
Background: Uroplakins (UP), urothelium-specific transmembrane proteins, are present only in urothelia and may be good candidates as tumor markers specific for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia and UP-II genes in the tissues and peripheral blood of patients...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yuasa, Takeshi [verfasserIn] Yoshiki, Tatsuhiro [verfasserIn] Isono, Takahiro [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Melbourne, Australia: Blackwell Science Pty ; 1999 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2002 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: International journal of urology - Oxford [u.a.] : Wiley-Blackwell, 1997, 6(1999), 6, Seite 0 |
| Übergeordnetes Werk: |
volume:6 ; year:1999 ; number:6 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1046/j.1442-2042.1999.00064.x |
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| Katalog-ID: |
NLEJ242673104 |
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| 100 | 1 | |a Yuasa, Takeshi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo] |
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| 520 | |a Background: Uroplakins (UP), urothelium-specific transmembrane proteins, are present only in urothelia and may be good candidates as tumor markers specific for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia and UP-II genes in the tissues and peripheral blood of patients with TCC. Methods: We investigated UP-Ia and UP-II gene expression in tissues from 12 patients with TCC by reverse transcription–polymerase chain reaction (RT-PCR). HT1197, a TCC cell line, was used as an indicated cell line to assess a detection system for the UP-II gene-expressing cancer cells by nested RT-PCR. We also investigated UP-II gene expression in the peripheral blood of 12 other patients with TCC by nested RT-PCR. Results: Prior to the investigation of UP-Ia and UP-II gene expression, a partial nucleotide sequence of human UP-II gene cDNA was determined to prepare the primers for RT-PCR. Uroplakin genes were expressed in both cancerous and non-cancerous urothelia taken from all patients examined by RT-PCR. The detection sensitivity of our assay showed that one cancer cell could be detected in 5 mL peripheral blood. UP-II gene-expression was detected in the peripheral blood from all three patients with metastatic TCC but not from the nine patients with non-metastatic TCC nor the three healthy volunteers. Conclusions: Uroplakins may be employed as tumor markers for transitional cell cancer, because they are highly conserved and well expressed, not only in non-cancerous cells but also in cancer cells. Furthermore, detection of UP-II gene expression in blood by nested RT-PCR may provide helpful information in the diagnosis and management of TCC. | ||
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| 700 | 1 | |a Yoshiki, Tatsuhiro |e verfasserin |4 aut | |
| 700 | 1 | |a Isono, Takahiro |e verfasserin |4 aut | |
| 700 | 1 | |a Tanaka, Tsutomu |4 oth | |
| 700 | 1 | |a Hayashida, Hideshi |4 oth | |
| 700 | 1 | |a Okada, Yusaku |4 oth | |
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10.1046/j.1442-2042.1999.00064.x doi (DE-627)NLEJ242673104 DE-627 ger DE-627 rakwb Yuasa, Takeshi verfasserin aut Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo] Melbourne, Australia Blackwell Science Pty 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Background: Uroplakins (UP), urothelium-specific transmembrane proteins, are present only in urothelia and may be good candidates as tumor markers specific for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia and UP-II genes in the tissues and peripheral blood of patients with TCC. Methods: We investigated UP-Ia and UP-II gene expression in tissues from 12 patients with TCC by reverse transcription–polymerase chain reaction (RT-PCR). HT1197, a TCC cell line, was used as an indicated cell line to assess a detection system for the UP-II gene-expressing cancer cells by nested RT-PCR. We also investigated UP-II gene expression in the peripheral blood of 12 other patients with TCC by nested RT-PCR. Results: Prior to the investigation of UP-Ia and UP-II gene expression, a partial nucleotide sequence of human UP-II gene cDNA was determined to prepare the primers for RT-PCR. Uroplakin genes were expressed in both cancerous and non-cancerous urothelia taken from all patients examined by RT-PCR. The detection sensitivity of our assay showed that one cancer cell could be detected in 5 mL peripheral blood. UP-II gene-expression was detected in the peripheral blood from all three patients with metastatic TCC but not from the nine patients with non-metastatic TCC nor the three healthy volunteers. Conclusions: Uroplakins may be employed as tumor markers for transitional cell cancer, because they are highly conserved and well expressed, not only in non-cancerous cells but also in cancer cells. Furthermore, detection of UP-II gene expression in blood by nested RT-PCR may provide helpful information in the diagnosis and management of TCC. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| reverse transcription–polymerase chain reaction Yoshiki, Tatsuhiro verfasserin aut Isono, Takahiro verfasserin aut Tanaka, Tsutomu oth Hayashida, Hideshi oth Okada, Yusaku oth In International journal of urology Oxford [u.a.] : Wiley-Blackwell, 1997 6(1999), 6, Seite 0 Online-Ressource (DE-627)NLEJ243925654 (DE-600)2009793-1 1442-2042 nnns volume:6 year:1999 number:6 pages:0 http://dx.doi.org/10.1046/j.1442-2042.1999.00064.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 6 1999 6 0 |
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10.1046/j.1442-2042.1999.00064.x doi (DE-627)NLEJ242673104 DE-627 ger DE-627 rakwb Yuasa, Takeshi verfasserin aut Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo] Melbourne, Australia Blackwell Science Pty 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Background: Uroplakins (UP), urothelium-specific transmembrane proteins, are present only in urothelia and may be good candidates as tumor markers specific for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia and UP-II genes in the tissues and peripheral blood of patients with TCC. Methods: We investigated UP-Ia and UP-II gene expression in tissues from 12 patients with TCC by reverse transcription–polymerase chain reaction (RT-PCR). HT1197, a TCC cell line, was used as an indicated cell line to assess a detection system for the UP-II gene-expressing cancer cells by nested RT-PCR. We also investigated UP-II gene expression in the peripheral blood of 12 other patients with TCC by nested RT-PCR. Results: Prior to the investigation of UP-Ia and UP-II gene expression, a partial nucleotide sequence of human UP-II gene cDNA was determined to prepare the primers for RT-PCR. Uroplakin genes were expressed in both cancerous and non-cancerous urothelia taken from all patients examined by RT-PCR. The detection sensitivity of our assay showed that one cancer cell could be detected in 5 mL peripheral blood. UP-II gene-expression was detected in the peripheral blood from all three patients with metastatic TCC but not from the nine patients with non-metastatic TCC nor the three healthy volunteers. Conclusions: Uroplakins may be employed as tumor markers for transitional cell cancer, because they are highly conserved and well expressed, not only in non-cancerous cells but also in cancer cells. Furthermore, detection of UP-II gene expression in blood by nested RT-PCR may provide helpful information in the diagnosis and management of TCC. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| reverse transcription–polymerase chain reaction Yoshiki, Tatsuhiro verfasserin aut Isono, Takahiro verfasserin aut Tanaka, Tsutomu oth Hayashida, Hideshi oth Okada, Yusaku oth In International journal of urology Oxford [u.a.] : Wiley-Blackwell, 1997 6(1999), 6, Seite 0 Online-Ressource (DE-627)NLEJ243925654 (DE-600)2009793-1 1442-2042 nnns volume:6 year:1999 number:6 pages:0 http://dx.doi.org/10.1046/j.1442-2042.1999.00064.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 6 1999 6 0 |
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10.1046/j.1442-2042.1999.00064.x doi (DE-627)NLEJ242673104 DE-627 ger DE-627 rakwb Yuasa, Takeshi verfasserin aut Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo] Melbourne, Australia Blackwell Science Pty 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Background: Uroplakins (UP), urothelium-specific transmembrane proteins, are present only in urothelia and may be good candidates as tumor markers specific for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia and UP-II genes in the tissues and peripheral blood of patients with TCC. Methods: We investigated UP-Ia and UP-II gene expression in tissues from 12 patients with TCC by reverse transcription–polymerase chain reaction (RT-PCR). HT1197, a TCC cell line, was used as an indicated cell line to assess a detection system for the UP-II gene-expressing cancer cells by nested RT-PCR. We also investigated UP-II gene expression in the peripheral blood of 12 other patients with TCC by nested RT-PCR. Results: Prior to the investigation of UP-Ia and UP-II gene expression, a partial nucleotide sequence of human UP-II gene cDNA was determined to prepare the primers for RT-PCR. Uroplakin genes were expressed in both cancerous and non-cancerous urothelia taken from all patients examined by RT-PCR. The detection sensitivity of our assay showed that one cancer cell could be detected in 5 mL peripheral blood. UP-II gene-expression was detected in the peripheral blood from all three patients with metastatic TCC but not from the nine patients with non-metastatic TCC nor the three healthy volunteers. Conclusions: Uroplakins may be employed as tumor markers for transitional cell cancer, because they are highly conserved and well expressed, not only in non-cancerous cells but also in cancer cells. Furthermore, detection of UP-II gene expression in blood by nested RT-PCR may provide helpful information in the diagnosis and management of TCC. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| reverse transcription–polymerase chain reaction Yoshiki, Tatsuhiro verfasserin aut Isono, Takahiro verfasserin aut Tanaka, Tsutomu oth Hayashida, Hideshi oth Okada, Yusaku oth In International journal of urology Oxford [u.a.] : Wiley-Blackwell, 1997 6(1999), 6, Seite 0 Online-Ressource (DE-627)NLEJ243925654 (DE-600)2009793-1 1442-2042 nnns volume:6 year:1999 number:6 pages:0 http://dx.doi.org/10.1046/j.1442-2042.1999.00064.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 6 1999 6 0 |
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10.1046/j.1442-2042.1999.00064.x doi (DE-627)NLEJ242673104 DE-627 ger DE-627 rakwb Yuasa, Takeshi verfasserin aut Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo] Melbourne, Australia Blackwell Science Pty 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Background: Uroplakins (UP), urothelium-specific transmembrane proteins, are present only in urothelia and may be good candidates as tumor markers specific for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia and UP-II genes in the tissues and peripheral blood of patients with TCC. Methods: We investigated UP-Ia and UP-II gene expression in tissues from 12 patients with TCC by reverse transcription–polymerase chain reaction (RT-PCR). HT1197, a TCC cell line, was used as an indicated cell line to assess a detection system for the UP-II gene-expressing cancer cells by nested RT-PCR. We also investigated UP-II gene expression in the peripheral blood of 12 other patients with TCC by nested RT-PCR. Results: Prior to the investigation of UP-Ia and UP-II gene expression, a partial nucleotide sequence of human UP-II gene cDNA was determined to prepare the primers for RT-PCR. Uroplakin genes were expressed in both cancerous and non-cancerous urothelia taken from all patients examined by RT-PCR. The detection sensitivity of our assay showed that one cancer cell could be detected in 5 mL peripheral blood. UP-II gene-expression was detected in the peripheral blood from all three patients with metastatic TCC but not from the nine patients with non-metastatic TCC nor the three healthy volunteers. Conclusions: Uroplakins may be employed as tumor markers for transitional cell cancer, because they are highly conserved and well expressed, not only in non-cancerous cells but also in cancer cells. Furthermore, detection of UP-II gene expression in blood by nested RT-PCR may provide helpful information in the diagnosis and management of TCC. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| reverse transcription–polymerase chain reaction Yoshiki, Tatsuhiro verfasserin aut Isono, Takahiro verfasserin aut Tanaka, Tsutomu oth Hayashida, Hideshi oth Okada, Yusaku oth In International journal of urology Oxford [u.a.] : Wiley-Blackwell, 1997 6(1999), 6, Seite 0 Online-Ressource (DE-627)NLEJ243925654 (DE-600)2009793-1 1442-2042 nnns volume:6 year:1999 number:6 pages:0 http://dx.doi.org/10.1046/j.1442-2042.1999.00064.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 6 1999 6 0 |
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10.1046/j.1442-2042.1999.00064.x doi (DE-627)NLEJ242673104 DE-627 ger DE-627 rakwb Yuasa, Takeshi verfasserin aut Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo] Melbourne, Australia Blackwell Science Pty 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Background: Uroplakins (UP), urothelium-specific transmembrane proteins, are present only in urothelia and may be good candidates as tumor markers specific for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia and UP-II genes in the tissues and peripheral blood of patients with TCC. Methods: We investigated UP-Ia and UP-II gene expression in tissues from 12 patients with TCC by reverse transcription–polymerase chain reaction (RT-PCR). HT1197, a TCC cell line, was used as an indicated cell line to assess a detection system for the UP-II gene-expressing cancer cells by nested RT-PCR. We also investigated UP-II gene expression in the peripheral blood of 12 other patients with TCC by nested RT-PCR. Results: Prior to the investigation of UP-Ia and UP-II gene expression, a partial nucleotide sequence of human UP-II gene cDNA was determined to prepare the primers for RT-PCR. Uroplakin genes were expressed in both cancerous and non-cancerous urothelia taken from all patients examined by RT-PCR. The detection sensitivity of our assay showed that one cancer cell could be detected in 5 mL peripheral blood. UP-II gene-expression was detected in the peripheral blood from all three patients with metastatic TCC but not from the nine patients with non-metastatic TCC nor the three healthy volunteers. Conclusions: Uroplakins may be employed as tumor markers for transitional cell cancer, because they are highly conserved and well expressed, not only in non-cancerous cells but also in cancer cells. Furthermore, detection of UP-II gene expression in blood by nested RT-PCR may provide helpful information in the diagnosis and management of TCC. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| reverse transcription–polymerase chain reaction Yoshiki, Tatsuhiro verfasserin aut Isono, Takahiro verfasserin aut Tanaka, Tsutomu oth Hayashida, Hideshi oth Okada, Yusaku oth In International journal of urology Oxford [u.a.] : Wiley-Blackwell, 1997 6(1999), 6, Seite 0 Online-Ressource (DE-627)NLEJ243925654 (DE-600)2009793-1 1442-2042 nnns volume:6 year:1999 number:6 pages:0 http://dx.doi.org/10.1046/j.1442-2042.1999.00064.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 6 1999 6 0 |
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Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo] |
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(DE-627)NLEJ242673104 |
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Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo] |
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Yuasa, Takeshi |
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International journal of urology |
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International journal of urology |
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1999 |
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Yuasa, Takeshi Yoshiki, Tatsuhiro Isono, Takahiro |
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6 |
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Online-Ressource |
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Elektronische Aufsätze |
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Yuasa, Takeshi |
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10.1046/j.1442-2042.1999.00064.x |
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verfasserin |
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expression of transitional cell-specific genes, uroplakin ia and ii, in bladder cancer: detection of circulating cancer cells in the peripheral blood of metastatic patients[the nucleo] |
| title_auth |
Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo] |
| abstract |
Background: Uroplakins (UP), urothelium-specific transmembrane proteins, are present only in urothelia and may be good candidates as tumor markers specific for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia and UP-II genes in the tissues and peripheral blood of patients with TCC. Methods: We investigated UP-Ia and UP-II gene expression in tissues from 12 patients with TCC by reverse transcription–polymerase chain reaction (RT-PCR). HT1197, a TCC cell line, was used as an indicated cell line to assess a detection system for the UP-II gene-expressing cancer cells by nested RT-PCR. We also investigated UP-II gene expression in the peripheral blood of 12 other patients with TCC by nested RT-PCR. Results: Prior to the investigation of UP-Ia and UP-II gene expression, a partial nucleotide sequence of human UP-II gene cDNA was determined to prepare the primers for RT-PCR. Uroplakin genes were expressed in both cancerous and non-cancerous urothelia taken from all patients examined by RT-PCR. The detection sensitivity of our assay showed that one cancer cell could be detected in 5 mL peripheral blood. UP-II gene-expression was detected in the peripheral blood from all three patients with metastatic TCC but not from the nine patients with non-metastatic TCC nor the three healthy volunteers. Conclusions: Uroplakins may be employed as tumor markers for transitional cell cancer, because they are highly conserved and well expressed, not only in non-cancerous cells but also in cancer cells. Furthermore, detection of UP-II gene expression in blood by nested RT-PCR may provide helpful information in the diagnosis and management of TCC. |
| abstractGer |
Background: Uroplakins (UP), urothelium-specific transmembrane proteins, are present only in urothelia and may be good candidates as tumor markers specific for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia and UP-II genes in the tissues and peripheral blood of patients with TCC. Methods: We investigated UP-Ia and UP-II gene expression in tissues from 12 patients with TCC by reverse transcription–polymerase chain reaction (RT-PCR). HT1197, a TCC cell line, was used as an indicated cell line to assess a detection system for the UP-II gene-expressing cancer cells by nested RT-PCR. We also investigated UP-II gene expression in the peripheral blood of 12 other patients with TCC by nested RT-PCR. Results: Prior to the investigation of UP-Ia and UP-II gene expression, a partial nucleotide sequence of human UP-II gene cDNA was determined to prepare the primers for RT-PCR. Uroplakin genes were expressed in both cancerous and non-cancerous urothelia taken from all patients examined by RT-PCR. The detection sensitivity of our assay showed that one cancer cell could be detected in 5 mL peripheral blood. UP-II gene-expression was detected in the peripheral blood from all three patients with metastatic TCC but not from the nine patients with non-metastatic TCC nor the three healthy volunteers. Conclusions: Uroplakins may be employed as tumor markers for transitional cell cancer, because they are highly conserved and well expressed, not only in non-cancerous cells but also in cancer cells. Furthermore, detection of UP-II gene expression in blood by nested RT-PCR may provide helpful information in the diagnosis and management of TCC. |
| abstract_unstemmed |
Background: Uroplakins (UP), urothelium-specific transmembrane proteins, are present only in urothelia and may be good candidates as tumor markers specific for transitional cell carcinomas (TCC). We investigated the expression of UP-Ia and UP-II genes in the tissues and peripheral blood of patients with TCC. Methods: We investigated UP-Ia and UP-II gene expression in tissues from 12 patients with TCC by reverse transcription–polymerase chain reaction (RT-PCR). HT1197, a TCC cell line, was used as an indicated cell line to assess a detection system for the UP-II gene-expressing cancer cells by nested RT-PCR. We also investigated UP-II gene expression in the peripheral blood of 12 other patients with TCC by nested RT-PCR. Results: Prior to the investigation of UP-Ia and UP-II gene expression, a partial nucleotide sequence of human UP-II gene cDNA was determined to prepare the primers for RT-PCR. Uroplakin genes were expressed in both cancerous and non-cancerous urothelia taken from all patients examined by RT-PCR. The detection sensitivity of our assay showed that one cancer cell could be detected in 5 mL peripheral blood. UP-II gene-expression was detected in the peripheral blood from all three patients with metastatic TCC but not from the nine patients with non-metastatic TCC nor the three healthy volunteers. Conclusions: Uroplakins may be employed as tumor markers for transitional cell cancer, because they are highly conserved and well expressed, not only in non-cancerous cells but also in cancer cells. Furthermore, detection of UP-II gene expression in blood by nested RT-PCR may provide helpful information in the diagnosis and management of TCC. |
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Expression of transitional cell-specific genes, uroplakin Ia and II, in bladder cancer: Detection of circulating cancer cells in the peripheral blood of metastatic patients[The nucleo] |
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http://dx.doi.org/10.1046/j.1442-2042.1999.00064.x |
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Yoshiki, Tatsuhiro Isono, Takahiro Tanaka, Tsutomu Hayashida, Hideshi Okada, Yusaku |
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Yoshiki, Tatsuhiro Isono, Takahiro Tanaka, Tsutomu Hayashida, Hideshi Okada, Yusaku |
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