Stress responses of linear plasmids from Debaryomyces hansenii
The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and ma...
Ausführliche Beschreibung
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| Autor*in: |
Fukuda, Kohsai [verfasserIn] Jin-Shan, Chen [verfasserIn] Kawano, Masagoro [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 2004 |
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Online-Ressource |
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2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: FEMS microbiology letters - Federation of European Microbiological Societies ; GKD-ID: 114439X, Oxford [u.a.] : Wiley-Blackwell, 1977, 237(2004), 2, Seite 0 |
| Übergeordnetes Werk: |
volume:237 ; year:2004 ; number:2 ; pages:0 |
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| DOI / URN: |
10.1111/j.1574-6968.2004.tb09702.x |
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| 520 | |a The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and maintenance. The degree of osmolarity dependence of pDHLs was greatly affected by growth temperature of the host cells: the stability of pDHLs was maintained in the absence of osmolarity in cells growing at 25 °C, and required osmorarity equivalent to 0.3–1.0 M NaCl on shifting to 30–35 °C. Although to less extent, similar osmolarity dependence at high temperatures was observed with another system of D. hansenii linear plasmids. Short-term conditioning of cells to heat or high osmolarity resulted in significant improvement in the plasmid stability, suggesting possible involvement of stress proteins and/or high glycerol level in the stabilization process. | ||
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10.1111/j.1574-6968.2004.tb09702.x doi (DE-627)NLEJ242876870 DE-627 ger DE-627 rakwb Fukuda, Kohsai verfasserin aut Stress responses of linear plasmids from Debaryomyces hansenii Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and maintenance. The degree of osmolarity dependence of pDHLs was greatly affected by growth temperature of the host cells: the stability of pDHLs was maintained in the absence of osmolarity in cells growing at 25 °C, and required osmorarity equivalent to 0.3–1.0 M NaCl on shifting to 30–35 °C. Although to less extent, similar osmolarity dependence at high temperatures was observed with another system of D. hansenii linear plasmids. Short-term conditioning of cells to heat or high osmolarity resulted in significant improvement in the plasmid stability, suggesting possible involvement of stress proteins and/or high glycerol level in the stabilization process. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Plasmid stability Jin-Shan, Chen verfasserin aut Kawano, Masagoro verfasserin aut Sudo, Kohichi oth Gunge, Norio oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 237(2004), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:237 year:2004 number:2 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2004.tb09702.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 237 2004 2 0 |
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10.1111/j.1574-6968.2004.tb09702.x doi (DE-627)NLEJ242876870 DE-627 ger DE-627 rakwb Fukuda, Kohsai verfasserin aut Stress responses of linear plasmids from Debaryomyces hansenii Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and maintenance. The degree of osmolarity dependence of pDHLs was greatly affected by growth temperature of the host cells: the stability of pDHLs was maintained in the absence of osmolarity in cells growing at 25 °C, and required osmorarity equivalent to 0.3–1.0 M NaCl on shifting to 30–35 °C. Although to less extent, similar osmolarity dependence at high temperatures was observed with another system of D. hansenii linear plasmids. Short-term conditioning of cells to heat or high osmolarity resulted in significant improvement in the plasmid stability, suggesting possible involvement of stress proteins and/or high glycerol level in the stabilization process. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Plasmid stability Jin-Shan, Chen verfasserin aut Kawano, Masagoro verfasserin aut Sudo, Kohichi oth Gunge, Norio oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 237(2004), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:237 year:2004 number:2 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2004.tb09702.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 237 2004 2 0 |
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10.1111/j.1574-6968.2004.tb09702.x doi (DE-627)NLEJ242876870 DE-627 ger DE-627 rakwb Fukuda, Kohsai verfasserin aut Stress responses of linear plasmids from Debaryomyces hansenii Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and maintenance. The degree of osmolarity dependence of pDHLs was greatly affected by growth temperature of the host cells: the stability of pDHLs was maintained in the absence of osmolarity in cells growing at 25 °C, and required osmorarity equivalent to 0.3–1.0 M NaCl on shifting to 30–35 °C. Although to less extent, similar osmolarity dependence at high temperatures was observed with another system of D. hansenii linear plasmids. Short-term conditioning of cells to heat or high osmolarity resulted in significant improvement in the plasmid stability, suggesting possible involvement of stress proteins and/or high glycerol level in the stabilization process. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Plasmid stability Jin-Shan, Chen verfasserin aut Kawano, Masagoro verfasserin aut Sudo, Kohichi oth Gunge, Norio oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 237(2004), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:237 year:2004 number:2 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2004.tb09702.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 237 2004 2 0 |
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10.1111/j.1574-6968.2004.tb09702.x doi (DE-627)NLEJ242876870 DE-627 ger DE-627 rakwb Fukuda, Kohsai verfasserin aut Stress responses of linear plasmids from Debaryomyces hansenii Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and maintenance. The degree of osmolarity dependence of pDHLs was greatly affected by growth temperature of the host cells: the stability of pDHLs was maintained in the absence of osmolarity in cells growing at 25 °C, and required osmorarity equivalent to 0.3–1.0 M NaCl on shifting to 30–35 °C. Although to less extent, similar osmolarity dependence at high temperatures was observed with another system of D. hansenii linear plasmids. Short-term conditioning of cells to heat or high osmolarity resulted in significant improvement in the plasmid stability, suggesting possible involvement of stress proteins and/or high glycerol level in the stabilization process. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Plasmid stability Jin-Shan, Chen verfasserin aut Kawano, Masagoro verfasserin aut Sudo, Kohichi oth Gunge, Norio oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 237(2004), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:237 year:2004 number:2 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2004.tb09702.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 237 2004 2 0 |
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10.1111/j.1574-6968.2004.tb09702.x doi (DE-627)NLEJ242876870 DE-627 ger DE-627 rakwb Fukuda, Kohsai verfasserin aut Stress responses of linear plasmids from Debaryomyces hansenii Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and maintenance. The degree of osmolarity dependence of pDHLs was greatly affected by growth temperature of the host cells: the stability of pDHLs was maintained in the absence of osmolarity in cells growing at 25 °C, and required osmorarity equivalent to 0.3–1.0 M NaCl on shifting to 30–35 °C. Although to less extent, similar osmolarity dependence at high temperatures was observed with another system of D. hansenii linear plasmids. Short-term conditioning of cells to heat or high osmolarity resulted in significant improvement in the plasmid stability, suggesting possible involvement of stress proteins and/or high glycerol level in the stabilization process. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Plasmid stability Jin-Shan, Chen verfasserin aut Kawano, Masagoro verfasserin aut Sudo, Kohichi oth Gunge, Norio oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 237(2004), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:237 year:2004 number:2 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2004.tb09702.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 237 2004 2 0 |
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The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and maintenance. The degree of osmolarity dependence of pDHLs was greatly affected by growth temperature of the host cells: the stability of pDHLs was maintained in the absence of osmolarity in cells growing at 25 °C, and required osmorarity equivalent to 0.3–1.0 M NaCl on shifting to 30–35 °C. Although to less extent, similar osmolarity dependence at high temperatures was observed with another system of D. hansenii linear plasmids. Short-term conditioning of cells to heat or high osmolarity resulted in significant improvement in the plasmid stability, suggesting possible involvement of stress proteins and/or high glycerol level in the stabilization process. |
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The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and maintenance. The degree of osmolarity dependence of pDHLs was greatly affected by growth temperature of the host cells: the stability of pDHLs was maintained in the absence of osmolarity in cells growing at 25 °C, and required osmorarity equivalent to 0.3–1.0 M NaCl on shifting to 30–35 °C. Although to less extent, similar osmolarity dependence at high temperatures was observed with another system of D. hansenii linear plasmids. Short-term conditioning of cells to heat or high osmolarity resulted in significant improvement in the plasmid stability, suggesting possible involvement of stress proteins and/or high glycerol level in the stabilization process. |
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The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and maintenance. The degree of osmolarity dependence of pDHLs was greatly affected by growth temperature of the host cells: the stability of pDHLs was maintained in the absence of osmolarity in cells growing at 25 °C, and required osmorarity equivalent to 0.3–1.0 M NaCl on shifting to 30–35 °C. Although to less extent, similar osmolarity dependence at high temperatures was observed with another system of D. hansenii linear plasmids. Short-term conditioning of cells to heat or high osmolarity resulted in significant improvement in the plasmid stability, suggesting possible involvement of stress proteins and/or high glycerol level in the stabilization process. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ242876870</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707165353.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120427s2004 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1574-6968.2004.tb09702.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ242876870</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Fukuda, Kohsai</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Stress responses of linear plasmids from Debaryomyces hansenii</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">2004</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The triplet linear plasmids pDHL1/2/3 from the salt-tolerant yeast Debaryomyces hansenii TK are localized in the cytoplasm and characterized by a unique feature that they require environmental stressors (0.3 M NaCl or solutes such as sorbitol with equivalent osmolarity) for stable replication and maintenance. The degree of osmolarity dependence of pDHLs was greatly affected by growth temperature of the host cells: the stability of pDHLs was maintained in the absence of osmolarity in cells growing at 25 °C, and required osmorarity equivalent to 0.3–1.0 M NaCl on shifting to 30–35 °C. Although to less extent, similar osmolarity dependence at high temperatures was observed with another system of D. hansenii linear plasmids. 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