Molecular diversity of live-attenuated prototypic vaccine strains and clinical isolates of Staphylococcus aureus
Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all labora...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Buzzola, Fernanda R. [verfasserIn] Quelle, Liliana S. [verfasserIn] Steele-Moore, Lynn [verfasserIn] |
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E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 2001 |
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Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: FEMS microbiology letters - Federation of European Microbiological Societies ; GKD-ID: 114439X, Oxford [u.a.] : Wiley-Blackwell, 1977, 202(2001), 1, Seite 0 |
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volume:202 ; year:2001 ; number:1 ; pages:0 |
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| DOI / URN: |
10.1111/j.1574-6968.2001.tb10785.x |
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| 520 | |a Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. This information is required before conducting field trials with the vaccine. | ||
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10.1111/j.1574-6968.2001.tb10785.x doi (DE-627)NLEJ242893422 DE-627 ger DE-627 rakwb Buzzola, Fernanda R. verfasserin aut Molecular diversity of live-attenuated prototypic vaccine strains and clinical isolates of Staphylococcus aureus Oxford, UK Blackwell Publishing Ltd 2001 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. This information is required before conducting field trials with the vaccine. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Live-attenuated vaccine Quelle, Liliana S. verfasserin aut Steele-Moore, Lynn verfasserin aut Berg, Donna oth Denamiel, Graciela oth Gentilini, Elida oth Sordelli, Daniel O. oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 202(2001), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:202 year:2001 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2001.tb10785.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 202 2001 1 0 |
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10.1111/j.1574-6968.2001.tb10785.x doi (DE-627)NLEJ242893422 DE-627 ger DE-627 rakwb Buzzola, Fernanda R. verfasserin aut Molecular diversity of live-attenuated prototypic vaccine strains and clinical isolates of Staphylococcus aureus Oxford, UK Blackwell Publishing Ltd 2001 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. This information is required before conducting field trials with the vaccine. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Live-attenuated vaccine Quelle, Liliana S. verfasserin aut Steele-Moore, Lynn verfasserin aut Berg, Donna oth Denamiel, Graciela oth Gentilini, Elida oth Sordelli, Daniel O. oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 202(2001), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:202 year:2001 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2001.tb10785.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 202 2001 1 0 |
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10.1111/j.1574-6968.2001.tb10785.x doi (DE-627)NLEJ242893422 DE-627 ger DE-627 rakwb Buzzola, Fernanda R. verfasserin aut Molecular diversity of live-attenuated prototypic vaccine strains and clinical isolates of Staphylococcus aureus Oxford, UK Blackwell Publishing Ltd 2001 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. This information is required before conducting field trials with the vaccine. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Live-attenuated vaccine Quelle, Liliana S. verfasserin aut Steele-Moore, Lynn verfasserin aut Berg, Donna oth Denamiel, Graciela oth Gentilini, Elida oth Sordelli, Daniel O. oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 202(2001), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:202 year:2001 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2001.tb10785.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 202 2001 1 0 |
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10.1111/j.1574-6968.2001.tb10785.x doi (DE-627)NLEJ242893422 DE-627 ger DE-627 rakwb Buzzola, Fernanda R. verfasserin aut Molecular diversity of live-attenuated prototypic vaccine strains and clinical isolates of Staphylococcus aureus Oxford, UK Blackwell Publishing Ltd 2001 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. This information is required before conducting field trials with the vaccine. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Live-attenuated vaccine Quelle, Liliana S. verfasserin aut Steele-Moore, Lynn verfasserin aut Berg, Donna oth Denamiel, Graciela oth Gentilini, Elida oth Sordelli, Daniel O. oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 202(2001), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:202 year:2001 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2001.tb10785.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 202 2001 1 0 |
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10.1111/j.1574-6968.2001.tb10785.x doi (DE-627)NLEJ242893422 DE-627 ger DE-627 rakwb Buzzola, Fernanda R. verfasserin aut Molecular diversity of live-attenuated prototypic vaccine strains and clinical isolates of Staphylococcus aureus Oxford, UK Blackwell Publishing Ltd 2001 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. This information is required before conducting field trials with the vaccine. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Live-attenuated vaccine Quelle, Liliana S. verfasserin aut Steele-Moore, Lynn verfasserin aut Berg, Donna oth Denamiel, Graciela oth Gentilini, Elida oth Sordelli, Daniel O. oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 202(2001), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:202 year:2001 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2001.tb10785.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 202 2001 1 0 |
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Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. This information is required before conducting field trials with the vaccine. |
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Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. This information is required before conducting field trials with the vaccine. |
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Bovine mastitis Staphylococcus aureus isolates and prototypic live-attenuated vaccine strains were analyzed by SmaI pulsed-field gel electrophoresis (PFGE) typing and automated ribotyping. The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. This information is required before conducting field trials with the vaccine. |
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The discriminatory index of these methods was 0.91 and 0.69, respectively. SmaI PFGE typing assigned all laboratory strains into cluster Q, which shared 49% similarity with clusters A and B, and 35% similarity with cluster C. Automated ribotyping placed laboratory strains within ribogroups different from those of bovine isolates. These methods have 70% concordance and permitted identification of the prototypic vaccine background from those of clinical isolates. 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