Role of SspA in the density-dependent expression of the transcriptional activator AarP in Providencia stuartii
The AarP protein in Providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2′)-Ia gene. In addition, AarP activates genes involved in a multiple antibiotic resistance (Mar) phenotype. Expression of an aarP–lacZ fusion incre...
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| Autor*in: |
Ding, Xuedong [verfasserIn] Baca-DeLancey, Rita R. [verfasserIn] Rather, Philip N. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 2001 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: FEMS microbiology letters - Federation of European Microbiological Societies ; GKD-ID: 114439X, Oxford [u.a.] : Wiley-Blackwell, 1977, 196(2001), 1, Seite 0 |
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volume:196 ; year:2001 ; number:1 ; pages:0 |
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| DOI / URN: |
10.1111/j.1574-6968.2001.tb10535.x |
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| 520 | |a The AarP protein in Providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2′)-Ia gene. In addition, AarP activates genes involved in a multiple antibiotic resistance (Mar) phenotype. Expression of an aarP–lacZ fusion increased in a density-dependent manner and reached peak levels at stationary phase. The expression of an aarP–lacZ fusion could be prematurely activated in cells at early to mid-exponential phase by the addition of spent culture supernatants from stationary phase cultures or by ethyl acetate extracts of these supernatants. Nutrient starvation had a negligible effect on aarP expression. In a search for mutations that block aarP activation at stationary phase, a mini-Tn5Cm insertion has been identified within a gene whose product was 77% identical to SspA, a regulatory protein involved in stationary phase gene expression and virulence. An unmarked sspA null allele (sspA2) was created by allelic replacement to further examine the role of sspA in P. stuartii. The sspA2 allele resulted in substantial decrease in aarP mRNA accumulation at various phases of growth. Furthermore, in an sspA mutant background, the aarP–lacZ fusion was no longer activated by an extracellular signal. | ||
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10.1111/j.1574-6968.2001.tb10535.x doi (DE-627)NLEJ242895832 DE-627 ger DE-627 rakwb Ding, Xuedong verfasserin aut Role of SspA in the density-dependent expression of the transcriptional activator AarP in Providencia stuartii Oxford, UK Blackwell Publishing Ltd 2001 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The AarP protein in Providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2′)-Ia gene. In addition, AarP activates genes involved in a multiple antibiotic resistance (Mar) phenotype. Expression of an aarP–lacZ fusion increased in a density-dependent manner and reached peak levels at stationary phase. The expression of an aarP–lacZ fusion could be prematurely activated in cells at early to mid-exponential phase by the addition of spent culture supernatants from stationary phase cultures or by ethyl acetate extracts of these supernatants. Nutrient starvation had a negligible effect on aarP expression. In a search for mutations that block aarP activation at stationary phase, a mini-Tn5Cm insertion has been identified within a gene whose product was 77% identical to SspA, a regulatory protein involved in stationary phase gene expression and virulence. An unmarked sspA null allele (sspA2) was created by allelic replacement to further examine the role of sspA in P. stuartii. The sspA2 allele resulted in substantial decrease in aarP mRNA accumulation at various phases of growth. Furthermore, in an sspA mutant background, the aarP–lacZ fusion was no longer activated by an extracellular signal. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Stationary phase Baca-DeLancey, Rita R. verfasserin aut Rather, Philip N. verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 196(2001), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:196 year:2001 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2001.tb10535.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 196 2001 1 0 |
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10.1111/j.1574-6968.2001.tb10535.x doi (DE-627)NLEJ242895832 DE-627 ger DE-627 rakwb Ding, Xuedong verfasserin aut Role of SspA in the density-dependent expression of the transcriptional activator AarP in Providencia stuartii Oxford, UK Blackwell Publishing Ltd 2001 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The AarP protein in Providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2′)-Ia gene. In addition, AarP activates genes involved in a multiple antibiotic resistance (Mar) phenotype. Expression of an aarP–lacZ fusion increased in a density-dependent manner and reached peak levels at stationary phase. The expression of an aarP–lacZ fusion could be prematurely activated in cells at early to mid-exponential phase by the addition of spent culture supernatants from stationary phase cultures or by ethyl acetate extracts of these supernatants. Nutrient starvation had a negligible effect on aarP expression. In a search for mutations that block aarP activation at stationary phase, a mini-Tn5Cm insertion has been identified within a gene whose product was 77% identical to SspA, a regulatory protein involved in stationary phase gene expression and virulence. An unmarked sspA null allele (sspA2) was created by allelic replacement to further examine the role of sspA in P. stuartii. The sspA2 allele resulted in substantial decrease in aarP mRNA accumulation at various phases of growth. Furthermore, in an sspA mutant background, the aarP–lacZ fusion was no longer activated by an extracellular signal. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Stationary phase Baca-DeLancey, Rita R. verfasserin aut Rather, Philip N. verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 196(2001), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:196 year:2001 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2001.tb10535.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 196 2001 1 0 |
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10.1111/j.1574-6968.2001.tb10535.x doi (DE-627)NLEJ242895832 DE-627 ger DE-627 rakwb Ding, Xuedong verfasserin aut Role of SspA in the density-dependent expression of the transcriptional activator AarP in Providencia stuartii Oxford, UK Blackwell Publishing Ltd 2001 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The AarP protein in Providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2′)-Ia gene. In addition, AarP activates genes involved in a multiple antibiotic resistance (Mar) phenotype. Expression of an aarP–lacZ fusion increased in a density-dependent manner and reached peak levels at stationary phase. The expression of an aarP–lacZ fusion could be prematurely activated in cells at early to mid-exponential phase by the addition of spent culture supernatants from stationary phase cultures or by ethyl acetate extracts of these supernatants. Nutrient starvation had a negligible effect on aarP expression. In a search for mutations that block aarP activation at stationary phase, a mini-Tn5Cm insertion has been identified within a gene whose product was 77% identical to SspA, a regulatory protein involved in stationary phase gene expression and virulence. An unmarked sspA null allele (sspA2) was created by allelic replacement to further examine the role of sspA in P. stuartii. The sspA2 allele resulted in substantial decrease in aarP mRNA accumulation at various phases of growth. Furthermore, in an sspA mutant background, the aarP–lacZ fusion was no longer activated by an extracellular signal. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Stationary phase Baca-DeLancey, Rita R. verfasserin aut Rather, Philip N. verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 196(2001), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:196 year:2001 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2001.tb10535.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 196 2001 1 0 |
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10.1111/j.1574-6968.2001.tb10535.x doi (DE-627)NLEJ242895832 DE-627 ger DE-627 rakwb Ding, Xuedong verfasserin aut Role of SspA in the density-dependent expression of the transcriptional activator AarP in Providencia stuartii Oxford, UK Blackwell Publishing Ltd 2001 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The AarP protein in Providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2′)-Ia gene. In addition, AarP activates genes involved in a multiple antibiotic resistance (Mar) phenotype. Expression of an aarP–lacZ fusion increased in a density-dependent manner and reached peak levels at stationary phase. The expression of an aarP–lacZ fusion could be prematurely activated in cells at early to mid-exponential phase by the addition of spent culture supernatants from stationary phase cultures or by ethyl acetate extracts of these supernatants. Nutrient starvation had a negligible effect on aarP expression. In a search for mutations that block aarP activation at stationary phase, a mini-Tn5Cm insertion has been identified within a gene whose product was 77% identical to SspA, a regulatory protein involved in stationary phase gene expression and virulence. An unmarked sspA null allele (sspA2) was created by allelic replacement to further examine the role of sspA in P. stuartii. The sspA2 allele resulted in substantial decrease in aarP mRNA accumulation at various phases of growth. Furthermore, in an sspA mutant background, the aarP–lacZ fusion was no longer activated by an extracellular signal. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Stationary phase Baca-DeLancey, Rita R. verfasserin aut Rather, Philip N. verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 196(2001), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:196 year:2001 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2001.tb10535.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 196 2001 1 0 |
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10.1111/j.1574-6968.2001.tb10535.x doi (DE-627)NLEJ242895832 DE-627 ger DE-627 rakwb Ding, Xuedong verfasserin aut Role of SspA in the density-dependent expression of the transcriptional activator AarP in Providencia stuartii Oxford, UK Blackwell Publishing Ltd 2001 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The AarP protein in Providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2′)-Ia gene. In addition, AarP activates genes involved in a multiple antibiotic resistance (Mar) phenotype. Expression of an aarP–lacZ fusion increased in a density-dependent manner and reached peak levels at stationary phase. The expression of an aarP–lacZ fusion could be prematurely activated in cells at early to mid-exponential phase by the addition of spent culture supernatants from stationary phase cultures or by ethyl acetate extracts of these supernatants. Nutrient starvation had a negligible effect on aarP expression. In a search for mutations that block aarP activation at stationary phase, a mini-Tn5Cm insertion has been identified within a gene whose product was 77% identical to SspA, a regulatory protein involved in stationary phase gene expression and virulence. An unmarked sspA null allele (sspA2) was created by allelic replacement to further examine the role of sspA in P. stuartii. The sspA2 allele resulted in substantial decrease in aarP mRNA accumulation at various phases of growth. Furthermore, in an sspA mutant background, the aarP–lacZ fusion was no longer activated by an extracellular signal. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Stationary phase Baca-DeLancey, Rita R. verfasserin aut Rather, Philip N. verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 196(2001), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:196 year:2001 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.2001.tb10535.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 196 2001 1 0 |
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Role of SspA in the density-dependent expression of the transcriptional activator AarP in Providencia stuartii |
| abstract |
The AarP protein in Providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2′)-Ia gene. In addition, AarP activates genes involved in a multiple antibiotic resistance (Mar) phenotype. Expression of an aarP–lacZ fusion increased in a density-dependent manner and reached peak levels at stationary phase. The expression of an aarP–lacZ fusion could be prematurely activated in cells at early to mid-exponential phase by the addition of spent culture supernatants from stationary phase cultures or by ethyl acetate extracts of these supernatants. Nutrient starvation had a negligible effect on aarP expression. In a search for mutations that block aarP activation at stationary phase, a mini-Tn5Cm insertion has been identified within a gene whose product was 77% identical to SspA, a regulatory protein involved in stationary phase gene expression and virulence. An unmarked sspA null allele (sspA2) was created by allelic replacement to further examine the role of sspA in P. stuartii. The sspA2 allele resulted in substantial decrease in aarP mRNA accumulation at various phases of growth. Furthermore, in an sspA mutant background, the aarP–lacZ fusion was no longer activated by an extracellular signal. |
| abstractGer |
The AarP protein in Providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2′)-Ia gene. In addition, AarP activates genes involved in a multiple antibiotic resistance (Mar) phenotype. Expression of an aarP–lacZ fusion increased in a density-dependent manner and reached peak levels at stationary phase. The expression of an aarP–lacZ fusion could be prematurely activated in cells at early to mid-exponential phase by the addition of spent culture supernatants from stationary phase cultures or by ethyl acetate extracts of these supernatants. Nutrient starvation had a negligible effect on aarP expression. In a search for mutations that block aarP activation at stationary phase, a mini-Tn5Cm insertion has been identified within a gene whose product was 77% identical to SspA, a regulatory protein involved in stationary phase gene expression and virulence. An unmarked sspA null allele (sspA2) was created by allelic replacement to further examine the role of sspA in P. stuartii. The sspA2 allele resulted in substantial decrease in aarP mRNA accumulation at various phases of growth. Furthermore, in an sspA mutant background, the aarP–lacZ fusion was no longer activated by an extracellular signal. |
| abstract_unstemmed |
The AarP protein in Providencia stuartii encodes a small transcriptional activator which activates the chromosomal aminoglycoside acetyltransferase aac(2′)-Ia gene. In addition, AarP activates genes involved in a multiple antibiotic resistance (Mar) phenotype. Expression of an aarP–lacZ fusion increased in a density-dependent manner and reached peak levels at stationary phase. The expression of an aarP–lacZ fusion could be prematurely activated in cells at early to mid-exponential phase by the addition of spent culture supernatants from stationary phase cultures or by ethyl acetate extracts of these supernatants. Nutrient starvation had a negligible effect on aarP expression. In a search for mutations that block aarP activation at stationary phase, a mini-Tn5Cm insertion has been identified within a gene whose product was 77% identical to SspA, a regulatory protein involved in stationary phase gene expression and virulence. An unmarked sspA null allele (sspA2) was created by allelic replacement to further examine the role of sspA in P. stuartii. The sspA2 allele resulted in substantial decrease in aarP mRNA accumulation at various phases of growth. Furthermore, in an sspA mutant background, the aarP–lacZ fusion was no longer activated by an extracellular signal. |
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Role of SspA in the density-dependent expression of the transcriptional activator AarP in Providencia stuartii |
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Baca-DeLancey, Rita R. Rather, Philip N. |
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