Cloning of the rplA gene encoding ribosomal protein L1 from Streptomyces aureofaciens and its transcriptional analysis in the course of differentiation
Using the method for the identification of promoters recognized by the sporulation-specific σ factor RpoZ, we identified a positive 882-bp Sau3AI DNA fragment in Streptomyces aureofaciens. Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a Mr of 259...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ján, Kormanec [verfasserIn] Renáta, Nováková [verfasserIn] L'uboš, Kl'učár [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1999 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: FEMS microbiology letters - Federation of European Microbiological Societies ; GKD-ID: 114439X, Oxford [u.a.] : Wiley-Blackwell, 1977, 178(1999), 1, Seite 0 |
| Übergeordnetes Werk: |
volume:178 ; year:1999 ; number:1 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1111/j.1574-6968.1999.tb13768.x |
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| 520 | |a Using the method for the identification of promoters recognized by the sporulation-specific σ factor RpoZ, we identified a positive 882-bp Sau3AI DNA fragment in Streptomyces aureofaciens. Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a Mr of 25974. The deduced protein product showed high sequence similarity to all ribosomal proteins L1, with maximum similarity to several Streptomyces spp. L1 (97% identity). The 5′-truncated rplK gene encoding ribosomal protein L11 was located upstream of the rplA gene. However, the putative rpoZ-dependent promoter, Pren25, was located divergently in the coding region of the rplA gene and was inactive in S. aureofaciens. To investigate expression of the rplA gene, Northern blot hybridization was performed using RNA prepared from S. aureofaciens in various developmental stages. These studies suggested that the rplA gene is expressed in all stages of differentiation as a 1400-nucleotides transcript. The amount of the transcript decreased during differentiation. | ||
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10.1111/j.1574-6968.1999.tb13768.x doi (DE-627)NLEJ242904556 DE-627 ger DE-627 rakwb Ján, Kormanec verfasserin aut Cloning of the rplA gene encoding ribosomal protein L1 from Streptomyces aureofaciens and its transcriptional analysis in the course of differentiation Oxford, UK Blackwell Publishing Ltd 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Using the method for the identification of promoters recognized by the sporulation-specific σ factor RpoZ, we identified a positive 882-bp Sau3AI DNA fragment in Streptomyces aureofaciens. Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a Mr of 25974. The deduced protein product showed high sequence similarity to all ribosomal proteins L1, with maximum similarity to several Streptomyces spp. L1 (97% identity). The 5′-truncated rplK gene encoding ribosomal protein L11 was located upstream of the rplA gene. However, the putative rpoZ-dependent promoter, Pren25, was located divergently in the coding region of the rplA gene and was inactive in S. aureofaciens. To investigate expression of the rplA gene, Northern blot hybridization was performed using RNA prepared from S. aureofaciens in various developmental stages. These studies suggested that the rplA gene is expressed in all stages of differentiation as a 1400-nucleotides transcript. The amount of the transcript decreased during differentiation. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Ribosomal protein Renáta, Nováková verfasserin aut L'uboš, Kl'učár verfasserin aut Dagmar, Homérová oth Beatrica, Ševčíková oth Ondrej, Sprušanský oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 178(1999), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:178 year:1999 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1999.tb13768.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 178 1999 1 0 |
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10.1111/j.1574-6968.1999.tb13768.x doi (DE-627)NLEJ242904556 DE-627 ger DE-627 rakwb Ján, Kormanec verfasserin aut Cloning of the rplA gene encoding ribosomal protein L1 from Streptomyces aureofaciens and its transcriptional analysis in the course of differentiation Oxford, UK Blackwell Publishing Ltd 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Using the method for the identification of promoters recognized by the sporulation-specific σ factor RpoZ, we identified a positive 882-bp Sau3AI DNA fragment in Streptomyces aureofaciens. Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a Mr of 25974. The deduced protein product showed high sequence similarity to all ribosomal proteins L1, with maximum similarity to several Streptomyces spp. L1 (97% identity). The 5′-truncated rplK gene encoding ribosomal protein L11 was located upstream of the rplA gene. However, the putative rpoZ-dependent promoter, Pren25, was located divergently in the coding region of the rplA gene and was inactive in S. aureofaciens. To investigate expression of the rplA gene, Northern blot hybridization was performed using RNA prepared from S. aureofaciens in various developmental stages. These studies suggested that the rplA gene is expressed in all stages of differentiation as a 1400-nucleotides transcript. The amount of the transcript decreased during differentiation. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Ribosomal protein Renáta, Nováková verfasserin aut L'uboš, Kl'učár verfasserin aut Dagmar, Homérová oth Beatrica, Ševčíková oth Ondrej, Sprušanský oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 178(1999), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:178 year:1999 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1999.tb13768.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 178 1999 1 0 |
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10.1111/j.1574-6968.1999.tb13768.x doi (DE-627)NLEJ242904556 DE-627 ger DE-627 rakwb Ján, Kormanec verfasserin aut Cloning of the rplA gene encoding ribosomal protein L1 from Streptomyces aureofaciens and its transcriptional analysis in the course of differentiation Oxford, UK Blackwell Publishing Ltd 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Using the method for the identification of promoters recognized by the sporulation-specific σ factor RpoZ, we identified a positive 882-bp Sau3AI DNA fragment in Streptomyces aureofaciens. Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a Mr of 25974. The deduced protein product showed high sequence similarity to all ribosomal proteins L1, with maximum similarity to several Streptomyces spp. L1 (97% identity). The 5′-truncated rplK gene encoding ribosomal protein L11 was located upstream of the rplA gene. However, the putative rpoZ-dependent promoter, Pren25, was located divergently in the coding region of the rplA gene and was inactive in S. aureofaciens. To investigate expression of the rplA gene, Northern blot hybridization was performed using RNA prepared from S. aureofaciens in various developmental stages. These studies suggested that the rplA gene is expressed in all stages of differentiation as a 1400-nucleotides transcript. The amount of the transcript decreased during differentiation. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Ribosomal protein Renáta, Nováková verfasserin aut L'uboš, Kl'učár verfasserin aut Dagmar, Homérová oth Beatrica, Ševčíková oth Ondrej, Sprušanský oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 178(1999), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:178 year:1999 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1999.tb13768.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 178 1999 1 0 |
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10.1111/j.1574-6968.1999.tb13768.x doi (DE-627)NLEJ242904556 DE-627 ger DE-627 rakwb Ján, Kormanec verfasserin aut Cloning of the rplA gene encoding ribosomal protein L1 from Streptomyces aureofaciens and its transcriptional analysis in the course of differentiation Oxford, UK Blackwell Publishing Ltd 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Using the method for the identification of promoters recognized by the sporulation-specific σ factor RpoZ, we identified a positive 882-bp Sau3AI DNA fragment in Streptomyces aureofaciens. Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a Mr of 25974. The deduced protein product showed high sequence similarity to all ribosomal proteins L1, with maximum similarity to several Streptomyces spp. L1 (97% identity). The 5′-truncated rplK gene encoding ribosomal protein L11 was located upstream of the rplA gene. However, the putative rpoZ-dependent promoter, Pren25, was located divergently in the coding region of the rplA gene and was inactive in S. aureofaciens. To investigate expression of the rplA gene, Northern blot hybridization was performed using RNA prepared from S. aureofaciens in various developmental stages. These studies suggested that the rplA gene is expressed in all stages of differentiation as a 1400-nucleotides transcript. The amount of the transcript decreased during differentiation. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Ribosomal protein Renáta, Nováková verfasserin aut L'uboš, Kl'učár verfasserin aut Dagmar, Homérová oth Beatrica, Ševčíková oth Ondrej, Sprušanský oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 178(1999), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:178 year:1999 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1999.tb13768.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 178 1999 1 0 |
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10.1111/j.1574-6968.1999.tb13768.x doi (DE-627)NLEJ242904556 DE-627 ger DE-627 rakwb Ján, Kormanec verfasserin aut Cloning of the rplA gene encoding ribosomal protein L1 from Streptomyces aureofaciens and its transcriptional analysis in the course of differentiation Oxford, UK Blackwell Publishing Ltd 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Using the method for the identification of promoters recognized by the sporulation-specific σ factor RpoZ, we identified a positive 882-bp Sau3AI DNA fragment in Streptomyces aureofaciens. Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a Mr of 25974. The deduced protein product showed high sequence similarity to all ribosomal proteins L1, with maximum similarity to several Streptomyces spp. L1 (97% identity). The 5′-truncated rplK gene encoding ribosomal protein L11 was located upstream of the rplA gene. However, the putative rpoZ-dependent promoter, Pren25, was located divergently in the coding region of the rplA gene and was inactive in S. aureofaciens. To investigate expression of the rplA gene, Northern blot hybridization was performed using RNA prepared from S. aureofaciens in various developmental stages. These studies suggested that the rplA gene is expressed in all stages of differentiation as a 1400-nucleotides transcript. The amount of the transcript decreased during differentiation. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Ribosomal protein Renáta, Nováková verfasserin aut L'uboš, Kl'učár verfasserin aut Dagmar, Homérová oth Beatrica, Ševčíková oth Ondrej, Sprušanský oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 178(1999), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:178 year:1999 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1999.tb13768.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 178 1999 1 0 |
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cloning of the rpla gene encoding ribosomal protein l1 from streptomyces aureofaciens and its transcriptional analysis in the course of differentiation |
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Cloning of the rplA gene encoding ribosomal protein L1 from Streptomyces aureofaciens and its transcriptional analysis in the course of differentiation |
| abstract |
Using the method for the identification of promoters recognized by the sporulation-specific σ factor RpoZ, we identified a positive 882-bp Sau3AI DNA fragment in Streptomyces aureofaciens. Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a Mr of 25974. The deduced protein product showed high sequence similarity to all ribosomal proteins L1, with maximum similarity to several Streptomyces spp. L1 (97% identity). The 5′-truncated rplK gene encoding ribosomal protein L11 was located upstream of the rplA gene. However, the putative rpoZ-dependent promoter, Pren25, was located divergently in the coding region of the rplA gene and was inactive in S. aureofaciens. To investigate expression of the rplA gene, Northern blot hybridization was performed using RNA prepared from S. aureofaciens in various developmental stages. These studies suggested that the rplA gene is expressed in all stages of differentiation as a 1400-nucleotides transcript. The amount of the transcript decreased during differentiation. |
| abstractGer |
Using the method for the identification of promoters recognized by the sporulation-specific σ factor RpoZ, we identified a positive 882-bp Sau3AI DNA fragment in Streptomyces aureofaciens. Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a Mr of 25974. The deduced protein product showed high sequence similarity to all ribosomal proteins L1, with maximum similarity to several Streptomyces spp. L1 (97% identity). The 5′-truncated rplK gene encoding ribosomal protein L11 was located upstream of the rplA gene. However, the putative rpoZ-dependent promoter, Pren25, was located divergently in the coding region of the rplA gene and was inactive in S. aureofaciens. To investigate expression of the rplA gene, Northern blot hybridization was performed using RNA prepared from S. aureofaciens in various developmental stages. These studies suggested that the rplA gene is expressed in all stages of differentiation as a 1400-nucleotides transcript. The amount of the transcript decreased during differentiation. |
| abstract_unstemmed |
Using the method for the identification of promoters recognized by the sporulation-specific σ factor RpoZ, we identified a positive 882-bp Sau3AI DNA fragment in Streptomyces aureofaciens. Sequence analysis revealed a complete open reading frame encoding a protein of 242 amino acids with a Mr of 25974. The deduced protein product showed high sequence similarity to all ribosomal proteins L1, with maximum similarity to several Streptomyces spp. L1 (97% identity). The 5′-truncated rplK gene encoding ribosomal protein L11 was located upstream of the rplA gene. However, the putative rpoZ-dependent promoter, Pren25, was located divergently in the coding region of the rplA gene and was inactive in S. aureofaciens. To investigate expression of the rplA gene, Northern blot hybridization was performed using RNA prepared from S. aureofaciens in various developmental stages. These studies suggested that the rplA gene is expressed in all stages of differentiation as a 1400-nucleotides transcript. The amount of the transcript decreased during differentiation. |
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Cloning of the rplA gene encoding ribosomal protein L1 from Streptomyces aureofaciens and its transcriptional analysis in the course of differentiation |
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Renáta, Nováková L'uboš, Kl'učár Dagmar, Homérová Beatrica, Ševčíková Ondrej, Sprušanský |
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Renáta, Nováková L'uboš, Kl'učár Dagmar, Homérová Beatrica, Ševčíková Ondrej, Sprušanský |
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