Enhanced invasion of Tyzzer's organism into cultured mouse hepatocytes by cytochalasin D
The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was signifi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kawamura, Seiji [verfasserIn] Yoshikawa, Yasuhiro [verfasserIn] Fujiwara, Kosaku [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1998 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: FEMS microbiology letters - Federation of European Microbiological Societies ; GKD-ID: 114439X, Oxford [u.a.] : Wiley-Blackwell, 1977, 160(1998), 1, Seite 0 |
| Übergeordnetes Werk: |
volume:160 ; year:1998 ; number:1 ; pages:0 |
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| DOI / URN: |
10.1111/j.1574-6968.1998.tb12897.x |
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| 520 | |a The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was significantly enhanced by cytochalasin D, a microfilament disrupting drug, and markedly suppressed by vinblastine, a microtubule inhibitor. Another microtubule inhibitor, colchicine, did not show any substantial effect. However, the cytoskeletal system of cultured mouse hepatocytes was sensitive to these three drugs, as judged by inhibition of FITC-dextran uptake. These results suggest that Tyzzer's organisms invade host cells by a unique mechanism that is suppressed by the normal functions of host cell microfilaments. | ||
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10.1111/j.1574-6968.1998.tb12897.x doi (DE-627)NLEJ242913563 DE-627 ger DE-627 rakwb Kawamura, Seiji verfasserin aut Enhanced invasion of Tyzzer's organism into cultured mouse hepatocytes by cytochalasin D Oxford, UK Blackwell Publishing Ltd 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was significantly enhanced by cytochalasin D, a microfilament disrupting drug, and markedly suppressed by vinblastine, a microtubule inhibitor. Another microtubule inhibitor, colchicine, did not show any substantial effect. However, the cytoskeletal system of cultured mouse hepatocytes was sensitive to these three drugs, as judged by inhibition of FITC-dextran uptake. These results suggest that Tyzzer's organisms invade host cells by a unique mechanism that is suppressed by the normal functions of host cell microfilaments. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Bacterium-eukaryotic cell interaction Yoshikawa, Yasuhiro verfasserin aut Fujiwara, Kosaku verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 160(1998), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:160 year:1998 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1998.tb12897.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 160 1998 1 0 |
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10.1111/j.1574-6968.1998.tb12897.x doi (DE-627)NLEJ242913563 DE-627 ger DE-627 rakwb Kawamura, Seiji verfasserin aut Enhanced invasion of Tyzzer's organism into cultured mouse hepatocytes by cytochalasin D Oxford, UK Blackwell Publishing Ltd 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was significantly enhanced by cytochalasin D, a microfilament disrupting drug, and markedly suppressed by vinblastine, a microtubule inhibitor. Another microtubule inhibitor, colchicine, did not show any substantial effect. However, the cytoskeletal system of cultured mouse hepatocytes was sensitive to these three drugs, as judged by inhibition of FITC-dextran uptake. These results suggest that Tyzzer's organisms invade host cells by a unique mechanism that is suppressed by the normal functions of host cell microfilaments. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Bacterium-eukaryotic cell interaction Yoshikawa, Yasuhiro verfasserin aut Fujiwara, Kosaku verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 160(1998), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:160 year:1998 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1998.tb12897.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 160 1998 1 0 |
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10.1111/j.1574-6968.1998.tb12897.x doi (DE-627)NLEJ242913563 DE-627 ger DE-627 rakwb Kawamura, Seiji verfasserin aut Enhanced invasion of Tyzzer's organism into cultured mouse hepatocytes by cytochalasin D Oxford, UK Blackwell Publishing Ltd 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was significantly enhanced by cytochalasin D, a microfilament disrupting drug, and markedly suppressed by vinblastine, a microtubule inhibitor. Another microtubule inhibitor, colchicine, did not show any substantial effect. However, the cytoskeletal system of cultured mouse hepatocytes was sensitive to these three drugs, as judged by inhibition of FITC-dextran uptake. These results suggest that Tyzzer's organisms invade host cells by a unique mechanism that is suppressed by the normal functions of host cell microfilaments. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Bacterium-eukaryotic cell interaction Yoshikawa, Yasuhiro verfasserin aut Fujiwara, Kosaku verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 160(1998), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:160 year:1998 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1998.tb12897.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 160 1998 1 0 |
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10.1111/j.1574-6968.1998.tb12897.x doi (DE-627)NLEJ242913563 DE-627 ger DE-627 rakwb Kawamura, Seiji verfasserin aut Enhanced invasion of Tyzzer's organism into cultured mouse hepatocytes by cytochalasin D Oxford, UK Blackwell Publishing Ltd 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was significantly enhanced by cytochalasin D, a microfilament disrupting drug, and markedly suppressed by vinblastine, a microtubule inhibitor. Another microtubule inhibitor, colchicine, did not show any substantial effect. However, the cytoskeletal system of cultured mouse hepatocytes was sensitive to these three drugs, as judged by inhibition of FITC-dextran uptake. These results suggest that Tyzzer's organisms invade host cells by a unique mechanism that is suppressed by the normal functions of host cell microfilaments. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Bacterium-eukaryotic cell interaction Yoshikawa, Yasuhiro verfasserin aut Fujiwara, Kosaku verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 160(1998), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:160 year:1998 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1998.tb12897.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 160 1998 1 0 |
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10.1111/j.1574-6968.1998.tb12897.x doi (DE-627)NLEJ242913563 DE-627 ger DE-627 rakwb Kawamura, Seiji verfasserin aut Enhanced invasion of Tyzzer's organism into cultured mouse hepatocytes by cytochalasin D Oxford, UK Blackwell Publishing Ltd 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was significantly enhanced by cytochalasin D, a microfilament disrupting drug, and markedly suppressed by vinblastine, a microtubule inhibitor. Another microtubule inhibitor, colchicine, did not show any substantial effect. However, the cytoskeletal system of cultured mouse hepatocytes was sensitive to these three drugs, as judged by inhibition of FITC-dextran uptake. These results suggest that Tyzzer's organisms invade host cells by a unique mechanism that is suppressed by the normal functions of host cell microfilaments. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Bacterium-eukaryotic cell interaction Yoshikawa, Yasuhiro verfasserin aut Fujiwara, Kosaku verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 160(1998), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:160 year:1998 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1998.tb12897.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 160 1998 1 0 |
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The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was significantly enhanced by cytochalasin D, a microfilament disrupting drug, and markedly suppressed by vinblastine, a microtubule inhibitor. Another microtubule inhibitor, colchicine, did not show any substantial effect. However, the cytoskeletal system of cultured mouse hepatocytes was sensitive to these three drugs, as judged by inhibition of FITC-dextran uptake. These results suggest that Tyzzer's organisms invade host cells by a unique mechanism that is suppressed by the normal functions of host cell microfilaments. |
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The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was significantly enhanced by cytochalasin D, a microfilament disrupting drug, and markedly suppressed by vinblastine, a microtubule inhibitor. Another microtubule inhibitor, colchicine, did not show any substantial effect. However, the cytoskeletal system of cultured mouse hepatocytes was sensitive to these three drugs, as judged by inhibition of FITC-dextran uptake. These results suggest that Tyzzer's organisms invade host cells by a unique mechanism that is suppressed by the normal functions of host cell microfilaments. |
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The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was significantly enhanced by cytochalasin D, a microfilament disrupting drug, and markedly suppressed by vinblastine, a microtubule inhibitor. Another microtubule inhibitor, colchicine, did not show any substantial effect. However, the cytoskeletal system of cultured mouse hepatocytes was sensitive to these three drugs, as judged by inhibition of FITC-dextran uptake. These results suggest that Tyzzer's organisms invade host cells by a unique mechanism that is suppressed by the normal functions of host cell microfilaments. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ242913563</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230505190900.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120427s1998 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1574-6968.1998.tb12897.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ242913563</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Kawamura, Seiji</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Enhanced invasion of Tyzzer's organism into cultured mouse hepatocytes by cytochalasin D</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1998</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The effects of cytoskeleton inhibitors on the invasion of Tyzzer's organism, an obligate intracellular bacterium, into cultured mouse hepatocytes were examined by double immunofluorescence observation and plaque assay. The two techniques gave comparable results. Invasion of bacteria was significantly enhanced by cytochalasin D, a microfilament disrupting drug, and markedly suppressed by vinblastine, a microtubule inhibitor. Another microtubule inhibitor, colchicine, did not show any substantial effect. However, the cytoskeletal system of cultured mouse hepatocytes was sensitive to these three drugs, as judged by inhibition of FITC-dextran uptake. 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