Isolation and characterization of a multiple peroxide resistant mutant from Xanthomonas campestris pv. phaseoli
We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more r...
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| Autor*in: |
Fuangthong, Mayuree [verfasserIn] Mongkolsuk, Skorn [verfasserIn] |
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E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1997 |
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Online-Ressource |
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2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: FEMS microbiology letters - Federation of European Microbiological Societies ; GKD-ID: 114439X, Oxford [u.a.] : Wiley-Blackwell, 1977, 152(1997), 1, Seite 0 |
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volume:152 ; year:1997 ; number:1 ; pages:0 |
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10.1111/j.1574-6968.1997.tb10427.x |
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| 245 | 1 | 0 | |a Isolation and characterization of a multiple peroxide resistant mutant from Xanthomonas campestris pv. phaseoli |
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| 520 | |a We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more resistance to tert-butyl hydroperoxide killing. Increases in activity were detected for the peroxide scavenging enzymes catalase (100-fold) and AhpC (over 30-fold). Also observed was cross-resistance to thermal killing; however, no cross-resistance to other oxidants or chemicals was found. Analysis of protein profiles revealed that proteins with molecular masses of 22 and 58 kDa were accumulated while proteins of 29, 33 and 41 kDa were depressed in the mutant. These results indicate that the mutant may have defect(s) in peroxide regulation, which resulted in high constitutive expression of peroxide scavenging enzymes. Nevertheless, the mutant retained growth phase dependent regulation of peroxide killing. The mutant should be useful in unravelling the nature of a complex peroxide stress regulon. | ||
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10.1111/j.1574-6968.1997.tb10427.x doi (DE-627)NLEJ242917712 DE-627 ger DE-627 rakwb Fuangthong, Mayuree verfasserin aut Isolation and characterization of a multiple peroxide resistant mutant from Xanthomonas campestris pv. phaseoli Oxford, UK Blackwell Publishing Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more resistance to tert-butyl hydroperoxide killing. Increases in activity were detected for the peroxide scavenging enzymes catalase (100-fold) and AhpC (over 30-fold). Also observed was cross-resistance to thermal killing; however, no cross-resistance to other oxidants or chemicals was found. Analysis of protein profiles revealed that proteins with molecular masses of 22 and 58 kDa were accumulated while proteins of 29, 33 and 41 kDa were depressed in the mutant. These results indicate that the mutant may have defect(s) in peroxide regulation, which resulted in high constitutive expression of peroxide scavenging enzymes. Nevertheless, the mutant retained growth phase dependent regulation of peroxide killing. The mutant should be useful in unravelling the nature of a complex peroxide stress regulon. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| H Mongkolsuk, Skorn verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 152(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:152 year:1997 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10427.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 152 1997 1 0 |
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10.1111/j.1574-6968.1997.tb10427.x doi (DE-627)NLEJ242917712 DE-627 ger DE-627 rakwb Fuangthong, Mayuree verfasserin aut Isolation and characterization of a multiple peroxide resistant mutant from Xanthomonas campestris pv. phaseoli Oxford, UK Blackwell Publishing Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more resistance to tert-butyl hydroperoxide killing. Increases in activity were detected for the peroxide scavenging enzymes catalase (100-fold) and AhpC (over 30-fold). Also observed was cross-resistance to thermal killing; however, no cross-resistance to other oxidants or chemicals was found. Analysis of protein profiles revealed that proteins with molecular masses of 22 and 58 kDa were accumulated while proteins of 29, 33 and 41 kDa were depressed in the mutant. These results indicate that the mutant may have defect(s) in peroxide regulation, which resulted in high constitutive expression of peroxide scavenging enzymes. Nevertheless, the mutant retained growth phase dependent regulation of peroxide killing. The mutant should be useful in unravelling the nature of a complex peroxide stress regulon. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| H Mongkolsuk, Skorn verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 152(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:152 year:1997 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10427.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 152 1997 1 0 |
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10.1111/j.1574-6968.1997.tb10427.x doi (DE-627)NLEJ242917712 DE-627 ger DE-627 rakwb Fuangthong, Mayuree verfasserin aut Isolation and characterization of a multiple peroxide resistant mutant from Xanthomonas campestris pv. phaseoli Oxford, UK Blackwell Publishing Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more resistance to tert-butyl hydroperoxide killing. Increases in activity were detected for the peroxide scavenging enzymes catalase (100-fold) and AhpC (over 30-fold). Also observed was cross-resistance to thermal killing; however, no cross-resistance to other oxidants or chemicals was found. Analysis of protein profiles revealed that proteins with molecular masses of 22 and 58 kDa were accumulated while proteins of 29, 33 and 41 kDa were depressed in the mutant. These results indicate that the mutant may have defect(s) in peroxide regulation, which resulted in high constitutive expression of peroxide scavenging enzymes. Nevertheless, the mutant retained growth phase dependent regulation of peroxide killing. The mutant should be useful in unravelling the nature of a complex peroxide stress regulon. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| H Mongkolsuk, Skorn verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 152(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:152 year:1997 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10427.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 152 1997 1 0 |
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10.1111/j.1574-6968.1997.tb10427.x doi (DE-627)NLEJ242917712 DE-627 ger DE-627 rakwb Fuangthong, Mayuree verfasserin aut Isolation and characterization of a multiple peroxide resistant mutant from Xanthomonas campestris pv. phaseoli Oxford, UK Blackwell Publishing Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more resistance to tert-butyl hydroperoxide killing. Increases in activity were detected for the peroxide scavenging enzymes catalase (100-fold) and AhpC (over 30-fold). Also observed was cross-resistance to thermal killing; however, no cross-resistance to other oxidants or chemicals was found. Analysis of protein profiles revealed that proteins with molecular masses of 22 and 58 kDa were accumulated while proteins of 29, 33 and 41 kDa were depressed in the mutant. These results indicate that the mutant may have defect(s) in peroxide regulation, which resulted in high constitutive expression of peroxide scavenging enzymes. Nevertheless, the mutant retained growth phase dependent regulation of peroxide killing. The mutant should be useful in unravelling the nature of a complex peroxide stress regulon. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| H Mongkolsuk, Skorn verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 152(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:152 year:1997 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10427.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 152 1997 1 0 |
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10.1111/j.1574-6968.1997.tb10427.x doi (DE-627)NLEJ242917712 DE-627 ger DE-627 rakwb Fuangthong, Mayuree verfasserin aut Isolation and characterization of a multiple peroxide resistant mutant from Xanthomonas campestris pv. phaseoli Oxford, UK Blackwell Publishing Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more resistance to tert-butyl hydroperoxide killing. Increases in activity were detected for the peroxide scavenging enzymes catalase (100-fold) and AhpC (over 30-fold). Also observed was cross-resistance to thermal killing; however, no cross-resistance to other oxidants or chemicals was found. Analysis of protein profiles revealed that proteins with molecular masses of 22 and 58 kDa were accumulated while proteins of 29, 33 and 41 kDa were depressed in the mutant. These results indicate that the mutant may have defect(s) in peroxide regulation, which resulted in high constitutive expression of peroxide scavenging enzymes. Nevertheless, the mutant retained growth phase dependent regulation of peroxide killing. The mutant should be useful in unravelling the nature of a complex peroxide stress regulon. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| H Mongkolsuk, Skorn verfasserin aut In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 152(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:152 year:1997 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10427.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 152 1997 1 0 |
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Isolation and characterization of a multiple peroxide resistant mutant from Xanthomonas campestris pv. phaseoli |
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We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more resistance to tert-butyl hydroperoxide killing. Increases in activity were detected for the peroxide scavenging enzymes catalase (100-fold) and AhpC (over 30-fold). Also observed was cross-resistance to thermal killing; however, no cross-resistance to other oxidants or chemicals was found. Analysis of protein profiles revealed that proteins with molecular masses of 22 and 58 kDa were accumulated while proteins of 29, 33 and 41 kDa were depressed in the mutant. These results indicate that the mutant may have defect(s) in peroxide regulation, which resulted in high constitutive expression of peroxide scavenging enzymes. Nevertheless, the mutant retained growth phase dependent regulation of peroxide killing. The mutant should be useful in unravelling the nature of a complex peroxide stress regulon. |
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We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more resistance to tert-butyl hydroperoxide killing. Increases in activity were detected for the peroxide scavenging enzymes catalase (100-fold) and AhpC (over 30-fold). Also observed was cross-resistance to thermal killing; however, no cross-resistance to other oxidants or chemicals was found. Analysis of protein profiles revealed that proteins with molecular masses of 22 and 58 kDa were accumulated while proteins of 29, 33 and 41 kDa were depressed in the mutant. These results indicate that the mutant may have defect(s) in peroxide regulation, which resulted in high constitutive expression of peroxide scavenging enzymes. Nevertheless, the mutant retained growth phase dependent regulation of peroxide killing. The mutant should be useful in unravelling the nature of a complex peroxide stress regulon. |
| abstract_unstemmed |
We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more resistance to tert-butyl hydroperoxide killing. Increases in activity were detected for the peroxide scavenging enzymes catalase (100-fold) and AhpC (over 30-fold). Also observed was cross-resistance to thermal killing; however, no cross-resistance to other oxidants or chemicals was found. Analysis of protein profiles revealed that proteins with molecular masses of 22 and 58 kDa were accumulated while proteins of 29, 33 and 41 kDa were depressed in the mutant. These results indicate that the mutant may have defect(s) in peroxide regulation, which resulted in high constitutive expression of peroxide scavenging enzymes. Nevertheless, the mutant retained growth phase dependent regulation of peroxide killing. The mutant should be useful in unravelling the nature of a complex peroxide stress regulon. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ242917712</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707165941.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120427s1997 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1574-6968.1997.tb10427.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ242917712</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Fuangthong, Mayuree</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Isolation and characterization of a multiple peroxide resistant mutant from Xanthomonas campestris pv. phaseoli</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1997</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">We have isolated a spontaneous multiple peroxide resistant Xanthomonas campestris pv. phaseoli mutant (Xp Hr). In the presence of peroxides, the mutant had a higher growth rate than the parent. It also had a greater than 100-fold increase in resistance levels to H2O2 killing but only slightly more resistance to tert-butyl hydroperoxide killing. Increases in activity were detected for the peroxide scavenging enzymes catalase (100-fold) and AhpC (over 30-fold). Also observed was cross-resistance to thermal killing; however, no cross-resistance to other oxidants or chemicals was found. Analysis of protein profiles revealed that proteins with molecular masses of 22 and 58 kDa were accumulated while proteins of 29, 33 and 41 kDa were depressed in the mutant. These results indicate that the mutant may have defect(s) in peroxide regulation, which resulted in high constitutive expression of peroxide scavenging enzymes. Nevertheless, the mutant retained growth phase dependent regulation of peroxide killing. The mutant should be useful in unravelling the nature of a complex peroxide stress regulon.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">H</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mongkolsuk, Skorn</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="a">Federation of European Microbiological Societies ; GKD-ID: 114439X</subfield><subfield code="t">FEMS microbiology letters</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1977</subfield><subfield code="g">152(1997), 1, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927053</subfield><subfield code="w">(DE-600)1501716-3</subfield><subfield code="x">1574-6968</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:152</subfield><subfield code="g">year:1997</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1574-6968.1997.tb10427.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">152</subfield><subfield code="j">1997</subfield><subfield code="e">1</subfield><subfield code="h">0</subfield></datafield></record></collection>
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