Description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora
A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-p...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Viegas, Cristina A [verfasserIn] Lilley, Andrew K [verfasserIn] Bruce, Ken [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 1997 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: FEMS microbiology letters - Federation of European Microbiological Societies ; GKD-ID: 114439X, Oxford [u.a.] : Wiley-Blackwell, 1977, 149(1997), 1, Seite 0 |
| Übergeordnetes Werk: |
volume:149 ; year:1997 ; number:1 ; pages:0 |
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| DOI / URN: |
10.1111/j.1574-6968.1997.tb10318.x |
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| 520 | |a A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. The oriV of pQBR11 was located within a unique sequence of 300 bp which initiated the replication of pUC derived suicide vectors in Pseudomonas putida UWC1. The limited size of the DNA sequence required to initiate replication, and the presence of two 15/16 bp directly repeated motifs, indicate that this group of mega-plasmids contain a single origin of replication, which initiates replication via a host-polymerase dependent rolling circle mechanism. | ||
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10.1111/j.1574-6968.1997.tb10318.x doi (DE-627)NLEJ242918948 DE-627 ger DE-627 rakwb Viegas, Cristina A verfasserin aut Description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora Oxford, UK Blackwell Publishing Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. The oriV of pQBR11 was located within a unique sequence of 300 bp which initiated the replication of pUC derived suicide vectors in Pseudomonas putida UWC1. The limited size of the DNA sequence required to initiate replication, and the presence of two 15/16 bp directly repeated motifs, indicate that this group of mega-plasmids contain a single origin of replication, which initiates replication via a host-polymerase dependent rolling circle mechanism. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Conjugative plasmid Lilley, Andrew K verfasserin aut Bruce, Ken verfasserin aut Bailey, Mark J oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 149(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:149 year:1997 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10318.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 149 1997 1 0 |
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10.1111/j.1574-6968.1997.tb10318.x doi (DE-627)NLEJ242918948 DE-627 ger DE-627 rakwb Viegas, Cristina A verfasserin aut Description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora Oxford, UK Blackwell Publishing Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. The oriV of pQBR11 was located within a unique sequence of 300 bp which initiated the replication of pUC derived suicide vectors in Pseudomonas putida UWC1. The limited size of the DNA sequence required to initiate replication, and the presence of two 15/16 bp directly repeated motifs, indicate that this group of mega-plasmids contain a single origin of replication, which initiates replication via a host-polymerase dependent rolling circle mechanism. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Conjugative plasmid Lilley, Andrew K verfasserin aut Bruce, Ken verfasserin aut Bailey, Mark J oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 149(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:149 year:1997 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10318.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 149 1997 1 0 |
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10.1111/j.1574-6968.1997.tb10318.x doi (DE-627)NLEJ242918948 DE-627 ger DE-627 rakwb Viegas, Cristina A verfasserin aut Description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora Oxford, UK Blackwell Publishing Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. The oriV of pQBR11 was located within a unique sequence of 300 bp which initiated the replication of pUC derived suicide vectors in Pseudomonas putida UWC1. The limited size of the DNA sequence required to initiate replication, and the presence of two 15/16 bp directly repeated motifs, indicate that this group of mega-plasmids contain a single origin of replication, which initiates replication via a host-polymerase dependent rolling circle mechanism. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Conjugative plasmid Lilley, Andrew K verfasserin aut Bruce, Ken verfasserin aut Bailey, Mark J oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 149(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:149 year:1997 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10318.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 149 1997 1 0 |
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10.1111/j.1574-6968.1997.tb10318.x doi (DE-627)NLEJ242918948 DE-627 ger DE-627 rakwb Viegas, Cristina A verfasserin aut Description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora Oxford, UK Blackwell Publishing Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. The oriV of pQBR11 was located within a unique sequence of 300 bp which initiated the replication of pUC derived suicide vectors in Pseudomonas putida UWC1. The limited size of the DNA sequence required to initiate replication, and the presence of two 15/16 bp directly repeated motifs, indicate that this group of mega-plasmids contain a single origin of replication, which initiates replication via a host-polymerase dependent rolling circle mechanism. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Conjugative plasmid Lilley, Andrew K verfasserin aut Bruce, Ken verfasserin aut Bailey, Mark J oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 149(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:149 year:1997 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10318.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 149 1997 1 0 |
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10.1111/j.1574-6968.1997.tb10318.x doi (DE-627)NLEJ242918948 DE-627 ger DE-627 rakwb Viegas, Cristina A verfasserin aut Description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora Oxford, UK Blackwell Publishing Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. The oriV of pQBR11 was located within a unique sequence of 300 bp which initiated the replication of pUC derived suicide vectors in Pseudomonas putida UWC1. The limited size of the DNA sequence required to initiate replication, and the presence of two 15/16 bp directly repeated motifs, indicate that this group of mega-plasmids contain a single origin of replication, which initiates replication via a host-polymerase dependent rolling circle mechanism. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Conjugative plasmid Lilley, Andrew K verfasserin aut Bruce, Ken verfasserin aut Bailey, Mark J oth In Federation of European Microbiological Societies ; GKD-ID: 114439X FEMS microbiology letters Oxford [u.a.] : Wiley-Blackwell, 1977 149(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927053 (DE-600)1501716-3 1574-6968 nnns volume:149 year:1997 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6968.1997.tb10318.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 149 1997 1 0 |
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Description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora |
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A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. The oriV of pQBR11 was located within a unique sequence of 300 bp which initiated the replication of pUC derived suicide vectors in Pseudomonas putida UWC1. The limited size of the DNA sequence required to initiate replication, and the presence of two 15/16 bp directly repeated motifs, indicate that this group of mega-plasmids contain a single origin of replication, which initiates replication via a host-polymerase dependent rolling circle mechanism. |
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A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. The oriV of pQBR11 was located within a unique sequence of 300 bp which initiated the replication of pUC derived suicide vectors in Pseudomonas putida UWC1. The limited size of the DNA sequence required to initiate replication, and the presence of two 15/16 bp directly repeated motifs, indicate that this group of mega-plasmids contain a single origin of replication, which initiates replication via a host-polymerase dependent rolling circle mechanism. |
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A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. The oriV of pQBR11 was located within a unique sequence of 300 bp which initiated the replication of pUC derived suicide vectors in Pseudomonas putida UWC1. The limited size of the DNA sequence required to initiate replication, and the presence of two 15/16 bp directly repeated motifs, indicate that this group of mega-plasmids contain a single origin of replication, which initiates replication via a host-polymerase dependent rolling circle mechanism. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ242918948</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707165951.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120427s1997 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1574-6968.1997.tb10318.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ242918948</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Viegas, Cristina A</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Description of a novel plasmid replicative origin from a genetically distinct family of conjugative plasmids associated with phytosphere microflora</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">1997</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A novel replicative origin (oriV) from a conjugative, mercury resistance plasmid (pQBR11, 304 kbp) has been cloned and sequenced. Homology to the pQBR11 oriV-containing 3.55 kbp BamHI fragment (pCV1200) was restricted to one of five genetically distinct classes (group I) of narrow host range, mega-plasmids that persist as a genetic component of the pseudomonad community indigenous to the microflora of sugar beet. The oriV of pQBR11 was located within a unique sequence of 300 bp which initiated the replication of pUC derived suicide vectors in Pseudomonas putida UWC1. The limited size of the DNA sequence required to initiate replication, and the presence of two 15/16 bp directly repeated motifs, indicate that this group of mega-plasmids contain a single origin of replication, which initiates replication via a host-polymerase dependent rolling circle mechanism.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2006</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2006||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Conjugative plasmid</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lilley, Andrew K</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bruce, Ken</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bailey, Mark J</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="a">Federation of European Microbiological Societies ; GKD-ID: 114439X</subfield><subfield code="t">FEMS microbiology letters</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1977</subfield><subfield code="g">149(1997), 1, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927053</subfield><subfield code="w">(DE-600)1501716-3</subfield><subfield code="x">1574-6968</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:149</subfield><subfield code="g">year:1997</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1574-6968.1997.tb10318.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">149</subfield><subfield code="j">1997</subfield><subfield code="e">1</subfield><subfield code="h">0</subfield></datafield></record></collection>
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7.4011927 |