Quantitative analysis of bacterial communities from Mediterranean sapropels based on cultivation-dependent methods
Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at...
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| Autor*in: |
Süß, Jacqueline [verfasserIn] Engelen, Bert [verfasserIn] Cypionka, Heribert [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 2004 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: FEMS microbiology ecology - Oxford [u.a.] : Wiley-Blackwell, 1990, 51(2004), 1, Seite 0 |
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volume:51 ; year:2004 ; number:1 ; pages:0 |
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| DOI / URN: |
10.1016/j.femsec.2004.07.010 |
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| 520 | |a Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. Highest MPN counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-chain fatty acids as sole carbon sources. These values reached up to 2 × 107 cm−3, representing 3.3% of the total cell count. A total of 98 pure cultures were isolated from the highest positive dilutions of the MPN series, representing the most abundant microorganisms culturable by the methods used. The strains were identified by molecular biological methods and could be grouped into 19 different phylotypes. They belonged to the α-, β-, γ-, and δ-Proteobacteria, to the Actinobacteria and the Firmicutes. However, about half of the number of isolates was closely related to the genera Photobacterium and Agrobacterium. Regarding the high cultivation success, these organisms can be assumed to be typical sapropel bacteria, representing a substantial part of the culturable indigenous microbial community. | ||
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10.1016/j.femsec.2004.07.010 doi (DE-627)NLEJ242946100 DE-627 ger DE-627 rakwb Süß, Jacqueline verfasserin aut Quantitative analysis of bacterial communities from Mediterranean sapropels based on cultivation-dependent methods Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. Highest MPN counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-chain fatty acids as sole carbon sources. These values reached up to 2 × 107 cm−3, representing 3.3% of the total cell count. A total of 98 pure cultures were isolated from the highest positive dilutions of the MPN series, representing the most abundant microorganisms culturable by the methods used. The strains were identified by molecular biological methods and could be grouped into 19 different phylotypes. They belonged to the α-, β-, γ-, and δ-Proteobacteria, to the Actinobacteria and the Firmicutes. However, about half of the number of isolates was closely related to the genera Photobacterium and Agrobacterium. Regarding the high cultivation success, these organisms can be assumed to be typical sapropel bacteria, representing a substantial part of the culturable indigenous microbial community. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Deep-sea sediment Engelen, Bert verfasserin aut Cypionka, Heribert verfasserin aut Sass, Henrik oth In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 51(2004), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:51 year:2004 number:1 pages:0 http://dx.doi.org/10.1016/j.femsec.2004.07.010 text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 51 2004 1 0 |
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10.1016/j.femsec.2004.07.010 doi (DE-627)NLEJ242946100 DE-627 ger DE-627 rakwb Süß, Jacqueline verfasserin aut Quantitative analysis of bacterial communities from Mediterranean sapropels based on cultivation-dependent methods Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. Highest MPN counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-chain fatty acids as sole carbon sources. These values reached up to 2 × 107 cm−3, representing 3.3% of the total cell count. A total of 98 pure cultures were isolated from the highest positive dilutions of the MPN series, representing the most abundant microorganisms culturable by the methods used. The strains were identified by molecular biological methods and could be grouped into 19 different phylotypes. They belonged to the α-, β-, γ-, and δ-Proteobacteria, to the Actinobacteria and the Firmicutes. However, about half of the number of isolates was closely related to the genera Photobacterium and Agrobacterium. Regarding the high cultivation success, these organisms can be assumed to be typical sapropel bacteria, representing a substantial part of the culturable indigenous microbial community. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Deep-sea sediment Engelen, Bert verfasserin aut Cypionka, Heribert verfasserin aut Sass, Henrik oth In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 51(2004), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:51 year:2004 number:1 pages:0 http://dx.doi.org/10.1016/j.femsec.2004.07.010 text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 51 2004 1 0 |
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10.1016/j.femsec.2004.07.010 doi (DE-627)NLEJ242946100 DE-627 ger DE-627 rakwb Süß, Jacqueline verfasserin aut Quantitative analysis of bacterial communities from Mediterranean sapropels based on cultivation-dependent methods Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. Highest MPN counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-chain fatty acids as sole carbon sources. These values reached up to 2 × 107 cm−3, representing 3.3% of the total cell count. A total of 98 pure cultures were isolated from the highest positive dilutions of the MPN series, representing the most abundant microorganisms culturable by the methods used. The strains were identified by molecular biological methods and could be grouped into 19 different phylotypes. They belonged to the α-, β-, γ-, and δ-Proteobacteria, to the Actinobacteria and the Firmicutes. However, about half of the number of isolates was closely related to the genera Photobacterium and Agrobacterium. Regarding the high cultivation success, these organisms can be assumed to be typical sapropel bacteria, representing a substantial part of the culturable indigenous microbial community. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Deep-sea sediment Engelen, Bert verfasserin aut Cypionka, Heribert verfasserin aut Sass, Henrik oth In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 51(2004), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:51 year:2004 number:1 pages:0 http://dx.doi.org/10.1016/j.femsec.2004.07.010 text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 51 2004 1 0 |
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10.1016/j.femsec.2004.07.010 doi (DE-627)NLEJ242946100 DE-627 ger DE-627 rakwb Süß, Jacqueline verfasserin aut Quantitative analysis of bacterial communities from Mediterranean sapropels based on cultivation-dependent methods Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. Highest MPN counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-chain fatty acids as sole carbon sources. These values reached up to 2 × 107 cm−3, representing 3.3% of the total cell count. A total of 98 pure cultures were isolated from the highest positive dilutions of the MPN series, representing the most abundant microorganisms culturable by the methods used. The strains were identified by molecular biological methods and could be grouped into 19 different phylotypes. They belonged to the α-, β-, γ-, and δ-Proteobacteria, to the Actinobacteria and the Firmicutes. However, about half of the number of isolates was closely related to the genera Photobacterium and Agrobacterium. Regarding the high cultivation success, these organisms can be assumed to be typical sapropel bacteria, representing a substantial part of the culturable indigenous microbial community. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Deep-sea sediment Engelen, Bert verfasserin aut Cypionka, Heribert verfasserin aut Sass, Henrik oth In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 51(2004), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:51 year:2004 number:1 pages:0 http://dx.doi.org/10.1016/j.femsec.2004.07.010 text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 51 2004 1 0 |
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10.1016/j.femsec.2004.07.010 doi (DE-627)NLEJ242946100 DE-627 ger DE-627 rakwb Süß, Jacqueline verfasserin aut Quantitative analysis of bacterial communities from Mediterranean sapropels based on cultivation-dependent methods Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. Highest MPN counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-chain fatty acids as sole carbon sources. These values reached up to 2 × 107 cm−3, representing 3.3% of the total cell count. A total of 98 pure cultures were isolated from the highest positive dilutions of the MPN series, representing the most abundant microorganisms culturable by the methods used. The strains were identified by molecular biological methods and could be grouped into 19 different phylotypes. They belonged to the α-, β-, γ-, and δ-Proteobacteria, to the Actinobacteria and the Firmicutes. However, about half of the number of isolates was closely related to the genera Photobacterium and Agrobacterium. Regarding the high cultivation success, these organisms can be assumed to be typical sapropel bacteria, representing a substantial part of the culturable indigenous microbial community. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Deep-sea sediment Engelen, Bert verfasserin aut Cypionka, Heribert verfasserin aut Sass, Henrik oth In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 51(2004), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:51 year:2004 number:1 pages:0 http://dx.doi.org/10.1016/j.femsec.2004.07.010 text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 51 2004 1 0 |
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Quantitative analysis of bacterial communities from Mediterranean sapropels based on cultivation-dependent methods |
| abstract |
Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. Highest MPN counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-chain fatty acids as sole carbon sources. These values reached up to 2 × 107 cm−3, representing 3.3% of the total cell count. A total of 98 pure cultures were isolated from the highest positive dilutions of the MPN series, representing the most abundant microorganisms culturable by the methods used. The strains were identified by molecular biological methods and could be grouped into 19 different phylotypes. They belonged to the α-, β-, γ-, and δ-Proteobacteria, to the Actinobacteria and the Firmicutes. However, about half of the number of isolates was closely related to the genera Photobacterium and Agrobacterium. Regarding the high cultivation success, these organisms can be assumed to be typical sapropel bacteria, representing a substantial part of the culturable indigenous microbial community. |
| abstractGer |
Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. Highest MPN counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-chain fatty acids as sole carbon sources. These values reached up to 2 × 107 cm−3, representing 3.3% of the total cell count. A total of 98 pure cultures were isolated from the highest positive dilutions of the MPN series, representing the most abundant microorganisms culturable by the methods used. The strains were identified by molecular biological methods and could be grouped into 19 different phylotypes. They belonged to the α-, β-, γ-, and δ-Proteobacteria, to the Actinobacteria and the Firmicutes. However, about half of the number of isolates was closely related to the genera Photobacterium and Agrobacterium. Regarding the high cultivation success, these organisms can be assumed to be typical sapropel bacteria, representing a substantial part of the culturable indigenous microbial community. |
| abstract_unstemmed |
Microbial communities of ancient Mediterranean sapropels, buried sediment layers of high organic matter, were analyzed by most probable number (MPN) approaches. Mineral media containing different carbon sources in sub-millimolar concentrations were used. MPN numbers were elevated in sapropels and at the sediment surface, which mirrored total cell count distributions. Highest MPN counts were obtained with a mixture of different monomeric and polymeric substrates, with amino acids or with long-chain fatty acids as sole carbon sources. These values reached up to 2 × 107 cm−3, representing 3.3% of the total cell count. A total of 98 pure cultures were isolated from the highest positive dilutions of the MPN series, representing the most abundant microorganisms culturable by the methods used. The strains were identified by molecular biological methods and could be grouped into 19 different phylotypes. They belonged to the α-, β-, γ-, and δ-Proteobacteria, to the Actinobacteria and the Firmicutes. However, about half of the number of isolates was closely related to the genera Photobacterium and Agrobacterium. Regarding the high cultivation success, these organisms can be assumed to be typical sapropel bacteria, representing a substantial part of the culturable indigenous microbial community. |
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Engelen, Bert Cypionka, Heribert Sass, Henrik |
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Engelen, Bert Cypionka, Heribert Sass, Henrik |
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NLEJ243926324 |
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false |
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| doi_str |
10.1016/j.femsec.2004.07.010 |
| up_date |
2024-07-06T03:46:08.437Z |
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1803799828562444288 |
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7.4013042 |