Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments
Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of archaeal community did not drastically cha...
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| Autor*in: |
Koizumi, Yoshikazu [verfasserIn] Takii, Susumu [verfasserIn] Fukui, Manabu [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 2004 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: FEMS microbiology ecology - Oxford [u.a.] : Wiley-Blackwell, 1990, 48(2004), 2, Seite 0 |
| Übergeordnetes Werk: |
volume:48 ; year:2004 ; number:2 ; pages:0 |
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| DOI / URN: |
10.1016/j.femsec.2004.02.013 |
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| 245 | 1 | 0 | |a Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments |
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| 520 | |a Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of archaeal community did not drastically change with depth. The archaeal 16S rRNA-based denaturing gradient gel electrophoresis band pattern was very similar to the 16S rDNA-based pattern. These results indicate that archaeal community structure does not drastically change throughout the 0–20 cm in depth, and most archaeal populations in the sediment retain extractable rRNA. Phylogenetic analysis of seven sequences retrieved from both DNA- and RNA-based denaturing gradient gel electrophoresis revealed that these sequences were divided into four major lineages; the kingdom Crenarchaeota, the order Methanomicrobiales, the family Methanosaetaceae, and an uncultured cluster within Euryarchaeota. The order Methanomicrobiales and the family Methanosaetaceae were the dominant methane-producing archaea in this profundal freshwater lake sediment. Moreover, we found Euryarchaeota that are not related to methanogens, and non-thermophilic Crenarchaeota. This suggests that archaeal populations other than methanogens in low-temperature sediment have been previously underestimated. | ||
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10.1016/j.femsec.2004.02.013 doi (DE-627)NLEJ242947255 DE-627 ger DE-627 rakwb Koizumi, Yoshikazu verfasserin aut Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of archaeal community did not drastically change with depth. The archaeal 16S rRNA-based denaturing gradient gel electrophoresis band pattern was very similar to the 16S rDNA-based pattern. These results indicate that archaeal community structure does not drastically change throughout the 0–20 cm in depth, and most archaeal populations in the sediment retain extractable rRNA. Phylogenetic analysis of seven sequences retrieved from both DNA- and RNA-based denaturing gradient gel electrophoresis revealed that these sequences were divided into four major lineages; the kingdom Crenarchaeota, the order Methanomicrobiales, the family Methanosaetaceae, and an uncultured cluster within Euryarchaeota. The order Methanomicrobiales and the family Methanosaetaceae were the dominant methane-producing archaea in this profundal freshwater lake sediment. Moreover, we found Euryarchaeota that are not related to methanogens, and non-thermophilic Crenarchaeota. This suggests that archaeal populations other than methanogens in low-temperature sediment have been previously underestimated. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Profundal freshwater lake sediment Takii, Susumu verfasserin aut Fukui, Manabu verfasserin aut In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 48(2004), 2, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:48 year:2004 number:2 pages:0 http://dx.doi.org/10.1016/j.femsec.2004.02.013 text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 48 2004 2 0 |
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10.1016/j.femsec.2004.02.013 doi (DE-627)NLEJ242947255 DE-627 ger DE-627 rakwb Koizumi, Yoshikazu verfasserin aut Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of archaeal community did not drastically change with depth. The archaeal 16S rRNA-based denaturing gradient gel electrophoresis band pattern was very similar to the 16S rDNA-based pattern. These results indicate that archaeal community structure does not drastically change throughout the 0–20 cm in depth, and most archaeal populations in the sediment retain extractable rRNA. Phylogenetic analysis of seven sequences retrieved from both DNA- and RNA-based denaturing gradient gel electrophoresis revealed that these sequences were divided into four major lineages; the kingdom Crenarchaeota, the order Methanomicrobiales, the family Methanosaetaceae, and an uncultured cluster within Euryarchaeota. The order Methanomicrobiales and the family Methanosaetaceae were the dominant methane-producing archaea in this profundal freshwater lake sediment. Moreover, we found Euryarchaeota that are not related to methanogens, and non-thermophilic Crenarchaeota. This suggests that archaeal populations other than methanogens in low-temperature sediment have been previously underestimated. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Profundal freshwater lake sediment Takii, Susumu verfasserin aut Fukui, Manabu verfasserin aut In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 48(2004), 2, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:48 year:2004 number:2 pages:0 http://dx.doi.org/10.1016/j.femsec.2004.02.013 text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 48 2004 2 0 |
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10.1016/j.femsec.2004.02.013 doi (DE-627)NLEJ242947255 DE-627 ger DE-627 rakwb Koizumi, Yoshikazu verfasserin aut Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of archaeal community did not drastically change with depth. The archaeal 16S rRNA-based denaturing gradient gel electrophoresis band pattern was very similar to the 16S rDNA-based pattern. These results indicate that archaeal community structure does not drastically change throughout the 0–20 cm in depth, and most archaeal populations in the sediment retain extractable rRNA. Phylogenetic analysis of seven sequences retrieved from both DNA- and RNA-based denaturing gradient gel electrophoresis revealed that these sequences were divided into four major lineages; the kingdom Crenarchaeota, the order Methanomicrobiales, the family Methanosaetaceae, and an uncultured cluster within Euryarchaeota. The order Methanomicrobiales and the family Methanosaetaceae were the dominant methane-producing archaea in this profundal freshwater lake sediment. Moreover, we found Euryarchaeota that are not related to methanogens, and non-thermophilic Crenarchaeota. This suggests that archaeal populations other than methanogens in low-temperature sediment have been previously underestimated. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Profundal freshwater lake sediment Takii, Susumu verfasserin aut Fukui, Manabu verfasserin aut In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 48(2004), 2, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:48 year:2004 number:2 pages:0 http://dx.doi.org/10.1016/j.femsec.2004.02.013 text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 48 2004 2 0 |
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10.1016/j.femsec.2004.02.013 doi (DE-627)NLEJ242947255 DE-627 ger DE-627 rakwb Koizumi, Yoshikazu verfasserin aut Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of archaeal community did not drastically change with depth. The archaeal 16S rRNA-based denaturing gradient gel electrophoresis band pattern was very similar to the 16S rDNA-based pattern. These results indicate that archaeal community structure does not drastically change throughout the 0–20 cm in depth, and most archaeal populations in the sediment retain extractable rRNA. Phylogenetic analysis of seven sequences retrieved from both DNA- and RNA-based denaturing gradient gel electrophoresis revealed that these sequences were divided into four major lineages; the kingdom Crenarchaeota, the order Methanomicrobiales, the family Methanosaetaceae, and an uncultured cluster within Euryarchaeota. The order Methanomicrobiales and the family Methanosaetaceae were the dominant methane-producing archaea in this profundal freshwater lake sediment. Moreover, we found Euryarchaeota that are not related to methanogens, and non-thermophilic Crenarchaeota. This suggests that archaeal populations other than methanogens in low-temperature sediment have been previously underestimated. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Profundal freshwater lake sediment Takii, Susumu verfasserin aut Fukui, Manabu verfasserin aut In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 48(2004), 2, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:48 year:2004 number:2 pages:0 http://dx.doi.org/10.1016/j.femsec.2004.02.013 text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 48 2004 2 0 |
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10.1016/j.femsec.2004.02.013 doi (DE-627)NLEJ242947255 DE-627 ger DE-627 rakwb Koizumi, Yoshikazu verfasserin aut Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments Oxford, UK Blackwell Publishing Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of archaeal community did not drastically change with depth. The archaeal 16S rRNA-based denaturing gradient gel electrophoresis band pattern was very similar to the 16S rDNA-based pattern. These results indicate that archaeal community structure does not drastically change throughout the 0–20 cm in depth, and most archaeal populations in the sediment retain extractable rRNA. Phylogenetic analysis of seven sequences retrieved from both DNA- and RNA-based denaturing gradient gel electrophoresis revealed that these sequences were divided into four major lineages; the kingdom Crenarchaeota, the order Methanomicrobiales, the family Methanosaetaceae, and an uncultured cluster within Euryarchaeota. The order Methanomicrobiales and the family Methanosaetaceae were the dominant methane-producing archaea in this profundal freshwater lake sediment. Moreover, we found Euryarchaeota that are not related to methanogens, and non-thermophilic Crenarchaeota. This suggests that archaeal populations other than methanogens in low-temperature sediment have been previously underestimated. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Profundal freshwater lake sediment Takii, Susumu verfasserin aut Fukui, Manabu verfasserin aut In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 48(2004), 2, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:48 year:2004 number:2 pages:0 http://dx.doi.org/10.1016/j.femsec.2004.02.013 text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 48 2004 2 0 |
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depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16s rrna genes and reversely transcribed rrna fragments |
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Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments |
| abstract |
Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of archaeal community did not drastically change with depth. The archaeal 16S rRNA-based denaturing gradient gel electrophoresis band pattern was very similar to the 16S rDNA-based pattern. These results indicate that archaeal community structure does not drastically change throughout the 0–20 cm in depth, and most archaeal populations in the sediment retain extractable rRNA. Phylogenetic analysis of seven sequences retrieved from both DNA- and RNA-based denaturing gradient gel electrophoresis revealed that these sequences were divided into four major lineages; the kingdom Crenarchaeota, the order Methanomicrobiales, the family Methanosaetaceae, and an uncultured cluster within Euryarchaeota. The order Methanomicrobiales and the family Methanosaetaceae were the dominant methane-producing archaea in this profundal freshwater lake sediment. Moreover, we found Euryarchaeota that are not related to methanogens, and non-thermophilic Crenarchaeota. This suggests that archaeal populations other than methanogens in low-temperature sediment have been previously underestimated. |
| abstractGer |
Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of archaeal community did not drastically change with depth. The archaeal 16S rRNA-based denaturing gradient gel electrophoresis band pattern was very similar to the 16S rDNA-based pattern. These results indicate that archaeal community structure does not drastically change throughout the 0–20 cm in depth, and most archaeal populations in the sediment retain extractable rRNA. Phylogenetic analysis of seven sequences retrieved from both DNA- and RNA-based denaturing gradient gel electrophoresis revealed that these sequences were divided into four major lineages; the kingdom Crenarchaeota, the order Methanomicrobiales, the family Methanosaetaceae, and an uncultured cluster within Euryarchaeota. The order Methanomicrobiales and the family Methanosaetaceae were the dominant methane-producing archaea in this profundal freshwater lake sediment. Moreover, we found Euryarchaeota that are not related to methanogens, and non-thermophilic Crenarchaeota. This suggests that archaeal populations other than methanogens in low-temperature sediment have been previously underestimated. |
| abstract_unstemmed |
Vertical changes in archaeal community structure in mesophilic freshwater lake sediment were investigated using denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed 16S rRNA fragments. Electrophoretic band pattern of archaeal community did not drastically change with depth. The archaeal 16S rRNA-based denaturing gradient gel electrophoresis band pattern was very similar to the 16S rDNA-based pattern. These results indicate that archaeal community structure does not drastically change throughout the 0–20 cm in depth, and most archaeal populations in the sediment retain extractable rRNA. Phylogenetic analysis of seven sequences retrieved from both DNA- and RNA-based denaturing gradient gel electrophoresis revealed that these sequences were divided into four major lineages; the kingdom Crenarchaeota, the order Methanomicrobiales, the family Methanosaetaceae, and an uncultured cluster within Euryarchaeota. The order Methanomicrobiales and the family Methanosaetaceae were the dominant methane-producing archaea in this profundal freshwater lake sediment. Moreover, we found Euryarchaeota that are not related to methanogens, and non-thermophilic Crenarchaeota. This suggests that archaeal populations other than methanogens in low-temperature sediment have been previously underestimated. |
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Depth-related change in archaeal community structure in a freshwater lake sediment as determined with denaturing gradient gel electrophoresis of amplified 16S rRNA genes and reversely transcribed rRNA fragments |
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Takii, Susumu Fukui, Manabu |
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