Influence of depth and sampling time on bacterial community structure in an upland grassland soil
Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and Au...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Griffiths, Robert I. [verfasserIn] Whiteley, Andrew S. [verfasserIn] O'Donnell, Anthony G. [verfasserIn] |
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Oxford, UK: Blackwell Publishing Ltd ; 2003 |
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Online-Ressource |
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2006 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: FEMS microbiology ecology - Oxford [u.a.] : Wiley-Blackwell, 1990, 43(2003), 1, Seite 0 |
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volume:43 ; year:2003 ; number:1 ; pages:0 |
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10.1111/j.1574-6941.2003.tb01043.x |
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| 520 | |a Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and August 2000. Cores were separated into four 5-cm depths to examine vertical spatial distribution. The 0–5-, 5–10- and 10–15-cm samples represented organic horizons whilst the 15–20-cm depths were from a mineral horizon. The potential metabolic activities were analysed using BIOLOG-GN plates, whereas genotypic diversity was evaluated using molecular profiling of amplified 16S rRNA and 16S rDNA gene fragments (denaturing gradient gel electrophoresis (DGGE)). BIOLOG-GN analysis revealed decreased substrate utilisation in the lowest depths, which was coupled with changes in the DNA and RNA DGGE profiles. Seasonal variation was pronounced in the 5–10-cm and 10–15-cm organic horizons for the July samplings whilst the 15–20-cm depths appeared more stable. Potential factors influencing the observed changes in bacterial communities resulting from soil depth and sampling time are discussed. | ||
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10.1111/j.1574-6941.2003.tb01043.x doi (DE-627)NLEJ242949282 DE-627 ger DE-627 rakwb Griffiths, Robert I. verfasserin aut Influence of depth and sampling time on bacterial community structure in an upland grassland soil Oxford, UK Blackwell Publishing Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and August 2000. Cores were separated into four 5-cm depths to examine vertical spatial distribution. The 0–5-, 5–10- and 10–15-cm samples represented organic horizons whilst the 15–20-cm depths were from a mineral horizon. The potential metabolic activities were analysed using BIOLOG-GN plates, whereas genotypic diversity was evaluated using molecular profiling of amplified 16S rRNA and 16S rDNA gene fragments (denaturing gradient gel electrophoresis (DGGE)). BIOLOG-GN analysis revealed decreased substrate utilisation in the lowest depths, which was coupled with changes in the DNA and RNA DGGE profiles. Seasonal variation was pronounced in the 5–10-cm and 10–15-cm organic horizons for the July samplings whilst the 15–20-cm depths appeared more stable. Potential factors influencing the observed changes in bacterial communities resulting from soil depth and sampling time are discussed. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Soil biodiversity Whiteley, Andrew S. verfasserin aut O'Donnell, Anthony G. verfasserin aut Bailey, Mark J. oth In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 43(2003), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:43 year:2003 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6941.2003.tb01043.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 43 2003 1 0 |
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10.1111/j.1574-6941.2003.tb01043.x doi (DE-627)NLEJ242949282 DE-627 ger DE-627 rakwb Griffiths, Robert I. verfasserin aut Influence of depth and sampling time on bacterial community structure in an upland grassland soil Oxford, UK Blackwell Publishing Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and August 2000. Cores were separated into four 5-cm depths to examine vertical spatial distribution. The 0–5-, 5–10- and 10–15-cm samples represented organic horizons whilst the 15–20-cm depths were from a mineral horizon. The potential metabolic activities were analysed using BIOLOG-GN plates, whereas genotypic diversity was evaluated using molecular profiling of amplified 16S rRNA and 16S rDNA gene fragments (denaturing gradient gel electrophoresis (DGGE)). BIOLOG-GN analysis revealed decreased substrate utilisation in the lowest depths, which was coupled with changes in the DNA and RNA DGGE profiles. Seasonal variation was pronounced in the 5–10-cm and 10–15-cm organic horizons for the July samplings whilst the 15–20-cm depths appeared more stable. Potential factors influencing the observed changes in bacterial communities resulting from soil depth and sampling time are discussed. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Soil biodiversity Whiteley, Andrew S. verfasserin aut O'Donnell, Anthony G. verfasserin aut Bailey, Mark J. oth In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 43(2003), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:43 year:2003 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6941.2003.tb01043.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 43 2003 1 0 |
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10.1111/j.1574-6941.2003.tb01043.x doi (DE-627)NLEJ242949282 DE-627 ger DE-627 rakwb Griffiths, Robert I. verfasserin aut Influence of depth and sampling time on bacterial community structure in an upland grassland soil Oxford, UK Blackwell Publishing Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and August 2000. Cores were separated into four 5-cm depths to examine vertical spatial distribution. The 0–5-, 5–10- and 10–15-cm samples represented organic horizons whilst the 15–20-cm depths were from a mineral horizon. The potential metabolic activities were analysed using BIOLOG-GN plates, whereas genotypic diversity was evaluated using molecular profiling of amplified 16S rRNA and 16S rDNA gene fragments (denaturing gradient gel electrophoresis (DGGE)). BIOLOG-GN analysis revealed decreased substrate utilisation in the lowest depths, which was coupled with changes in the DNA and RNA DGGE profiles. Seasonal variation was pronounced in the 5–10-cm and 10–15-cm organic horizons for the July samplings whilst the 15–20-cm depths appeared more stable. Potential factors influencing the observed changes in bacterial communities resulting from soil depth and sampling time are discussed. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Soil biodiversity Whiteley, Andrew S. verfasserin aut O'Donnell, Anthony G. verfasserin aut Bailey, Mark J. oth In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 43(2003), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:43 year:2003 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6941.2003.tb01043.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 43 2003 1 0 |
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10.1111/j.1574-6941.2003.tb01043.x doi (DE-627)NLEJ242949282 DE-627 ger DE-627 rakwb Griffiths, Robert I. verfasserin aut Influence of depth and sampling time on bacterial community structure in an upland grassland soil Oxford, UK Blackwell Publishing Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and August 2000. Cores were separated into four 5-cm depths to examine vertical spatial distribution. The 0–5-, 5–10- and 10–15-cm samples represented organic horizons whilst the 15–20-cm depths were from a mineral horizon. The potential metabolic activities were analysed using BIOLOG-GN plates, whereas genotypic diversity was evaluated using molecular profiling of amplified 16S rRNA and 16S rDNA gene fragments (denaturing gradient gel electrophoresis (DGGE)). BIOLOG-GN analysis revealed decreased substrate utilisation in the lowest depths, which was coupled with changes in the DNA and RNA DGGE profiles. Seasonal variation was pronounced in the 5–10-cm and 10–15-cm organic horizons for the July samplings whilst the 15–20-cm depths appeared more stable. Potential factors influencing the observed changes in bacterial communities resulting from soil depth and sampling time are discussed. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Soil biodiversity Whiteley, Andrew S. verfasserin aut O'Donnell, Anthony G. verfasserin aut Bailey, Mark J. oth In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 43(2003), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:43 year:2003 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6941.2003.tb01043.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 43 2003 1 0 |
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10.1111/j.1574-6941.2003.tb01043.x doi (DE-627)NLEJ242949282 DE-627 ger DE-627 rakwb Griffiths, Robert I. verfasserin aut Influence of depth and sampling time on bacterial community structure in an upland grassland soil Oxford, UK Blackwell Publishing Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and August 2000. Cores were separated into four 5-cm depths to examine vertical spatial distribution. The 0–5-, 5–10- and 10–15-cm samples represented organic horizons whilst the 15–20-cm depths were from a mineral horizon. The potential metabolic activities were analysed using BIOLOG-GN plates, whereas genotypic diversity was evaluated using molecular profiling of amplified 16S rRNA and 16S rDNA gene fragments (denaturing gradient gel electrophoresis (DGGE)). BIOLOG-GN analysis revealed decreased substrate utilisation in the lowest depths, which was coupled with changes in the DNA and RNA DGGE profiles. Seasonal variation was pronounced in the 5–10-cm and 10–15-cm organic horizons for the July samplings whilst the 15–20-cm depths appeared more stable. Potential factors influencing the observed changes in bacterial communities resulting from soil depth and sampling time are discussed. 2006 Blackwell Publishing Journal Backfiles 1879-2005 |2006|||||||||| Soil biodiversity Whiteley, Andrew S. verfasserin aut O'Donnell, Anthony G. verfasserin aut Bailey, Mark J. oth In FEMS microbiology ecology Oxford [u.a.] : Wiley-Blackwell, 1990 43(2003), 1, Seite 0 Online-Ressource (DE-627)NLEJ243926324 (DE-600)1501712-6 1574-6941 nnns volume:43 year:2003 number:1 pages:0 http://dx.doi.org/10.1111/j.1574-6941.2003.tb01043.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 43 2003 1 0 |
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Influence of depth and sampling time on bacterial community structure in an upland grassland soil |
| abstract |
Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and August 2000. Cores were separated into four 5-cm depths to examine vertical spatial distribution. The 0–5-, 5–10- and 10–15-cm samples represented organic horizons whilst the 15–20-cm depths were from a mineral horizon. The potential metabolic activities were analysed using BIOLOG-GN plates, whereas genotypic diversity was evaluated using molecular profiling of amplified 16S rRNA and 16S rDNA gene fragments (denaturing gradient gel electrophoresis (DGGE)). BIOLOG-GN analysis revealed decreased substrate utilisation in the lowest depths, which was coupled with changes in the DNA and RNA DGGE profiles. Seasonal variation was pronounced in the 5–10-cm and 10–15-cm organic horizons for the July samplings whilst the 15–20-cm depths appeared more stable. Potential factors influencing the observed changes in bacterial communities resulting from soil depth and sampling time are discussed. |
| abstractGer |
Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and August 2000. Cores were separated into four 5-cm depths to examine vertical spatial distribution. The 0–5-, 5–10- and 10–15-cm samples represented organic horizons whilst the 15–20-cm depths were from a mineral horizon. The potential metabolic activities were analysed using BIOLOG-GN plates, whereas genotypic diversity was evaluated using molecular profiling of amplified 16S rRNA and 16S rDNA gene fragments (denaturing gradient gel electrophoresis (DGGE)). BIOLOG-GN analysis revealed decreased substrate utilisation in the lowest depths, which was coupled with changes in the DNA and RNA DGGE profiles. Seasonal variation was pronounced in the 5–10-cm and 10–15-cm organic horizons for the July samplings whilst the 15–20-cm depths appeared more stable. Potential factors influencing the observed changes in bacterial communities resulting from soil depth and sampling time are discussed. |
| abstract_unstemmed |
Temporal and spatial variation of soil bacterial communities was evaluated with both molecular and metabolic profiling techniques. Soil cores (20 cm deep) were taken from an upland grassland in the Scottish Borders (UK) over 3 days in July 1999, and on single days in October 1999, April 2000, and August 2000. Cores were separated into four 5-cm depths to examine vertical spatial distribution. The 0–5-, 5–10- and 10–15-cm samples represented organic horizons whilst the 15–20-cm depths were from a mineral horizon. The potential metabolic activities were analysed using BIOLOG-GN plates, whereas genotypic diversity was evaluated using molecular profiling of amplified 16S rRNA and 16S rDNA gene fragments (denaturing gradient gel electrophoresis (DGGE)). BIOLOG-GN analysis revealed decreased substrate utilisation in the lowest depths, which was coupled with changes in the DNA and RNA DGGE profiles. Seasonal variation was pronounced in the 5–10-cm and 10–15-cm organic horizons for the July samplings whilst the 15–20-cm depths appeared more stable. Potential factors influencing the observed changes in bacterial communities resulting from soil depth and sampling time are discussed. |
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Whiteley, Andrew S. O'Donnell, Anthony G. Bailey, Mark J. |
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10.1111/j.1574-6941.2003.tb01043.x |
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