GENETIC SCREENING FOR IDENTIFICATION OF SALMONELLA TYPHIMURIUM TN5 MUTANTS WITH POTENTIAL HYPERSENSITIVITY TO SHORT-CHAIN FATTY ACIDS
This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic condi...
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| Autor*in: |
KWON, Y.M. [verfasserIn] KUBENA, L.F. [verfasserIn] NISBET, D.J. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 2003 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2007 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Journal of rapid methods and automation in microbiology - Oxford [u.a.] : Wiley-Blackwell, 1992, 11(2003), 2, Seite 0 |
| Übergeordnetes Werk: |
volume:11 ; year:2003 ; number:2 ; pages:0 |
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| DOI / URN: |
10.1111/j.1745-4581.2003.tb00032.x |
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| 520 | |a This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic conditions in the animal intestine that has been suggested to play a role in inhibiting colonization by nonindigenous bacterial pathogens. When the mutant pools containing 25 Tn5 mutants/pool were analyzed for transposon footprints before and after selection, a polymerase chain reaction (PCR) product could be identified that was present in an input pool, but not in a corresponding output pool. The results indicate that transposon footprinting can be used for negative screening of genes sensitive to SCFA in the S. typhimurium bacterial genome. | ||
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10.1111/j.1745-4581.2003.tb00032.x doi (DE-627)NLEJ242979262 DE-627 ger DE-627 rakwb KWON, Y.M. verfasserin aut GENETIC SCREENING FOR IDENTIFICATION OF SALMONELLA TYPHIMURIUM TN5 MUTANTS WITH POTENTIAL HYPERSENSITIVITY TO SHORT-CHAIN FATTY ACIDS Oxford, UK Blackwell Publishing Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic conditions in the animal intestine that has been suggested to play a role in inhibiting colonization by nonindigenous bacterial pathogens. When the mutant pools containing 25 Tn5 mutants/pool were analyzed for transposon footprints before and after selection, a polymerase chain reaction (PCR) product could be identified that was present in an input pool, but not in a corresponding output pool. The results indicate that transposon footprinting can be used for negative screening of genes sensitive to SCFA in the S. typhimurium bacterial genome. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| KUBENA, L.F. verfasserin aut NISBET, D.J. verfasserin aut RICKE, S.C. oth In Journal of rapid methods and automation in microbiology Oxford [u.a.] : Wiley-Blackwell, 1992 11(2003), 2, Seite 0 Online-Ressource (DE-627)NLEJ243926030 (DE-600)2176280-6 1745-4581 nnns volume:11 year:2003 number:2 pages:0 http://dx.doi.org/10.1111/j.1745-4581.2003.tb00032.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 11 2003 2 0 |
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10.1111/j.1745-4581.2003.tb00032.x doi (DE-627)NLEJ242979262 DE-627 ger DE-627 rakwb KWON, Y.M. verfasserin aut GENETIC SCREENING FOR IDENTIFICATION OF SALMONELLA TYPHIMURIUM TN5 MUTANTS WITH POTENTIAL HYPERSENSITIVITY TO SHORT-CHAIN FATTY ACIDS Oxford, UK Blackwell Publishing Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic conditions in the animal intestine that has been suggested to play a role in inhibiting colonization by nonindigenous bacterial pathogens. When the mutant pools containing 25 Tn5 mutants/pool were analyzed for transposon footprints before and after selection, a polymerase chain reaction (PCR) product could be identified that was present in an input pool, but not in a corresponding output pool. The results indicate that transposon footprinting can be used for negative screening of genes sensitive to SCFA in the S. typhimurium bacterial genome. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| KUBENA, L.F. verfasserin aut NISBET, D.J. verfasserin aut RICKE, S.C. oth In Journal of rapid methods and automation in microbiology Oxford [u.a.] : Wiley-Blackwell, 1992 11(2003), 2, Seite 0 Online-Ressource (DE-627)NLEJ243926030 (DE-600)2176280-6 1745-4581 nnns volume:11 year:2003 number:2 pages:0 http://dx.doi.org/10.1111/j.1745-4581.2003.tb00032.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 11 2003 2 0 |
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10.1111/j.1745-4581.2003.tb00032.x doi (DE-627)NLEJ242979262 DE-627 ger DE-627 rakwb KWON, Y.M. verfasserin aut GENETIC SCREENING FOR IDENTIFICATION OF SALMONELLA TYPHIMURIUM TN5 MUTANTS WITH POTENTIAL HYPERSENSITIVITY TO SHORT-CHAIN FATTY ACIDS Oxford, UK Blackwell Publishing Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic conditions in the animal intestine that has been suggested to play a role in inhibiting colonization by nonindigenous bacterial pathogens. When the mutant pools containing 25 Tn5 mutants/pool were analyzed for transposon footprints before and after selection, a polymerase chain reaction (PCR) product could be identified that was present in an input pool, but not in a corresponding output pool. The results indicate that transposon footprinting can be used for negative screening of genes sensitive to SCFA in the S. typhimurium bacterial genome. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| KUBENA, L.F. verfasserin aut NISBET, D.J. verfasserin aut RICKE, S.C. oth In Journal of rapid methods and automation in microbiology Oxford [u.a.] : Wiley-Blackwell, 1992 11(2003), 2, Seite 0 Online-Ressource (DE-627)NLEJ243926030 (DE-600)2176280-6 1745-4581 nnns volume:11 year:2003 number:2 pages:0 http://dx.doi.org/10.1111/j.1745-4581.2003.tb00032.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 11 2003 2 0 |
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10.1111/j.1745-4581.2003.tb00032.x doi (DE-627)NLEJ242979262 DE-627 ger DE-627 rakwb KWON, Y.M. verfasserin aut GENETIC SCREENING FOR IDENTIFICATION OF SALMONELLA TYPHIMURIUM TN5 MUTANTS WITH POTENTIAL HYPERSENSITIVITY TO SHORT-CHAIN FATTY ACIDS Oxford, UK Blackwell Publishing Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic conditions in the animal intestine that has been suggested to play a role in inhibiting colonization by nonindigenous bacterial pathogens. When the mutant pools containing 25 Tn5 mutants/pool were analyzed for transposon footprints before and after selection, a polymerase chain reaction (PCR) product could be identified that was present in an input pool, but not in a corresponding output pool. The results indicate that transposon footprinting can be used for negative screening of genes sensitive to SCFA in the S. typhimurium bacterial genome. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| KUBENA, L.F. verfasserin aut NISBET, D.J. verfasserin aut RICKE, S.C. oth In Journal of rapid methods and automation in microbiology Oxford [u.a.] : Wiley-Blackwell, 1992 11(2003), 2, Seite 0 Online-Ressource (DE-627)NLEJ243926030 (DE-600)2176280-6 1745-4581 nnns volume:11 year:2003 number:2 pages:0 http://dx.doi.org/10.1111/j.1745-4581.2003.tb00032.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 11 2003 2 0 |
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10.1111/j.1745-4581.2003.tb00032.x doi (DE-627)NLEJ242979262 DE-627 ger DE-627 rakwb KWON, Y.M. verfasserin aut GENETIC SCREENING FOR IDENTIFICATION OF SALMONELLA TYPHIMURIUM TN5 MUTANTS WITH POTENTIAL HYPERSENSITIVITY TO SHORT-CHAIN FATTY ACIDS Oxford, UK Blackwell Publishing Ltd 2003 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic conditions in the animal intestine that has been suggested to play a role in inhibiting colonization by nonindigenous bacterial pathogens. When the mutant pools containing 25 Tn5 mutants/pool were analyzed for transposon footprints before and after selection, a polymerase chain reaction (PCR) product could be identified that was present in an input pool, but not in a corresponding output pool. The results indicate that transposon footprinting can be used for negative screening of genes sensitive to SCFA in the S. typhimurium bacterial genome. 2007 Blackwell Publishing Journal Backfiles 1879-2005 |2007|||||||||| KUBENA, L.F. verfasserin aut NISBET, D.J. verfasserin aut RICKE, S.C. oth In Journal of rapid methods and automation in microbiology Oxford [u.a.] : Wiley-Blackwell, 1992 11(2003), 2, Seite 0 Online-Ressource (DE-627)NLEJ243926030 (DE-600)2176280-6 1745-4581 nnns volume:11 year:2003 number:2 pages:0 http://dx.doi.org/10.1111/j.1745-4581.2003.tb00032.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 11 2003 2 0 |
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GENETIC SCREENING FOR IDENTIFICATION OF SALMONELLA TYPHIMURIUM TN5 MUTANTS WITH POTENTIAL HYPERSENSITIVITY TO SHORT-CHAIN FATTY ACIDS |
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This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic conditions in the animal intestine that has been suggested to play a role in inhibiting colonization by nonindigenous bacterial pathogens. When the mutant pools containing 25 Tn5 mutants/pool were analyzed for transposon footprints before and after selection, a polymerase chain reaction (PCR) product could be identified that was present in an input pool, but not in a corresponding output pool. The results indicate that transposon footprinting can be used for negative screening of genes sensitive to SCFA in the S. typhimurium bacterial genome. |
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This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic conditions in the animal intestine that has been suggested to play a role in inhibiting colonization by nonindigenous bacterial pathogens. When the mutant pools containing 25 Tn5 mutants/pool were analyzed for transposon footprints before and after selection, a polymerase chain reaction (PCR) product could be identified that was present in an input pool, but not in a corresponding output pool. The results indicate that transposon footprinting can be used for negative screening of genes sensitive to SCFA in the S. typhimurium bacterial genome. |
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This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic conditions in the animal intestine that has been suggested to play a role in inhibiting colonization by nonindigenous bacterial pathogens. When the mutant pools containing 25 Tn5 mutants/pool were analyzed for transposon footprints before and after selection, a polymerase chain reaction (PCR) product could be identified that was present in an input pool, but not in a corresponding output pool. The results indicate that transposon footprinting can be used for negative screening of genes sensitive to SCFA in the S. typhimurium bacterial genome. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ242979262</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707170821.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120427s2003 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1745-4581.2003.tb00032.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ242979262</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">KWON, Y.M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">GENETIC SCREENING FOR IDENTIFICATION OF SALMONELLA TYPHIMURIUM TN5 MUTANTS WITH POTENTIAL HYPERSENSITIVITY TO SHORT-CHAIN FATTY ACIDS</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Blackwell Publishing Ltd</subfield><subfield code="c">2003</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">This study was conducted to test if transposon footprinting could be used to identify transposon mutants of Salmonella typhimurium with growth defects in a media containing short-chain fatty acids (SCFA) as the test selective condition. High concentrations of SCFA are one of the characteristic conditions in the animal intestine that has been suggested to play a role in inhibiting colonization by nonindigenous bacterial pathogens. When the mutant pools containing 25 Tn5 mutants/pool were analyzed for transposon footprints before and after selection, a polymerase chain reaction (PCR) product could be identified that was present in an input pool, but not in a corresponding output pool. The results indicate that transposon footprinting can be used for negative screening of genes sensitive to SCFA in the S. typhimurium bacterial genome.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2007</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2007||||||||||</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">KUBENA, L.F.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">NISBET, D.J.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">RICKE, S.C.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of rapid methods and automation in microbiology</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1992</subfield><subfield code="g">11(2003), 2, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243926030</subfield><subfield code="w">(DE-600)2176280-6</subfield><subfield code="x">1745-4581</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:11</subfield><subfield code="g">year:2003</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1745-4581.2003.tb00032.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">11</subfield><subfield code="j">2003</subfield><subfield code="e">2</subfield><subfield code="h">0</subfield></datafield></record></collection>
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