Immunohistochemical co-localization of lymphatics and blood vessels in oral squamous cell carcinomas
Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in o...
Ausführliche Beschreibung
Autor*in: |
Xuan, Ming [verfasserIn] Fang, Yi-Ru [verfasserIn] Wato, Masahiro [verfasserIn] |
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E-Artikel |
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Erschienen: |
Oxford, UK: Munksgaard International Publishers ; 2005 |
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Online-Ressource |
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Reproduktion: |
2005 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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Übergeordnetes Werk: |
In: Journal of oral pathology & medicine - Oxford [u.a.] : Wiley-Blackwell, 1972, 34(2005), 6, Seite 0 |
Übergeordnetes Werk: |
volume:34 ; year:2005 ; number:6 ; pages:0 |
Links: |
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DOI / URN: |
10.1111/j.1600-0714.2005.00316.x |
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NLEJ243033184 |
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520 | |a Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.Methods: Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with 5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′-diaminobenzidine (DAB) as color reagents, respectively.Results: In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.Conclusions: These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral. | ||
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700 | 1 | |a Tanaka, Akio |4 oth | |
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10.1111/j.1600-0714.2005.00316.x doi (DE-627)NLEJ243033184 DE-627 ger DE-627 rakwb Xuan, Ming verfasserin aut Immunohistochemical co-localization of lymphatics and blood vessels in oral squamous cell carcinomas Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.Methods: Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with 5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′-diaminobenzidine (DAB) as color reagents, respectively.Results: In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.Conclusions: These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| blood vessels Fang, Yi-Ru verfasserin aut Wato, Masahiro verfasserin aut Hata, Shintaro oth Tanaka, Akio oth In Journal of oral pathology & medicine Oxford [u.a.] : Wiley-Blackwell, 1972 34(2005), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927231 (DE-600)2026385-5 1600-0714 nnns volume:34 year:2005 number:6 pages:0 http://dx.doi.org/10.1111/j.1600-0714.2005.00316.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 34 2005 6 0 |
spelling |
10.1111/j.1600-0714.2005.00316.x doi (DE-627)NLEJ243033184 DE-627 ger DE-627 rakwb Xuan, Ming verfasserin aut Immunohistochemical co-localization of lymphatics and blood vessels in oral squamous cell carcinomas Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.Methods: Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with 5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′-diaminobenzidine (DAB) as color reagents, respectively.Results: In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.Conclusions: These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| blood vessels Fang, Yi-Ru verfasserin aut Wato, Masahiro verfasserin aut Hata, Shintaro oth Tanaka, Akio oth In Journal of oral pathology & medicine Oxford [u.a.] : Wiley-Blackwell, 1972 34(2005), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927231 (DE-600)2026385-5 1600-0714 nnns volume:34 year:2005 number:6 pages:0 http://dx.doi.org/10.1111/j.1600-0714.2005.00316.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 34 2005 6 0 |
allfields_unstemmed |
10.1111/j.1600-0714.2005.00316.x doi (DE-627)NLEJ243033184 DE-627 ger DE-627 rakwb Xuan, Ming verfasserin aut Immunohistochemical co-localization of lymphatics and blood vessels in oral squamous cell carcinomas Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.Methods: Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with 5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′-diaminobenzidine (DAB) as color reagents, respectively.Results: In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.Conclusions: These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| blood vessels Fang, Yi-Ru verfasserin aut Wato, Masahiro verfasserin aut Hata, Shintaro oth Tanaka, Akio oth In Journal of oral pathology & medicine Oxford [u.a.] : Wiley-Blackwell, 1972 34(2005), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927231 (DE-600)2026385-5 1600-0714 nnns volume:34 year:2005 number:6 pages:0 http://dx.doi.org/10.1111/j.1600-0714.2005.00316.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 34 2005 6 0 |
allfieldsGer |
10.1111/j.1600-0714.2005.00316.x doi (DE-627)NLEJ243033184 DE-627 ger DE-627 rakwb Xuan, Ming verfasserin aut Immunohistochemical co-localization of lymphatics and blood vessels in oral squamous cell carcinomas Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.Methods: Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with 5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′-diaminobenzidine (DAB) as color reagents, respectively.Results: In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.Conclusions: These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| blood vessels Fang, Yi-Ru verfasserin aut Wato, Masahiro verfasserin aut Hata, Shintaro oth Tanaka, Akio oth In Journal of oral pathology & medicine Oxford [u.a.] : Wiley-Blackwell, 1972 34(2005), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927231 (DE-600)2026385-5 1600-0714 nnns volume:34 year:2005 number:6 pages:0 http://dx.doi.org/10.1111/j.1600-0714.2005.00316.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 34 2005 6 0 |
allfieldsSound |
10.1111/j.1600-0714.2005.00316.x doi (DE-627)NLEJ243033184 DE-627 ger DE-627 rakwb Xuan, Ming verfasserin aut Immunohistochemical co-localization of lymphatics and blood vessels in oral squamous cell carcinomas Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.Methods: Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with 5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′-diaminobenzidine (DAB) as color reagents, respectively.Results: In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.Conclusions: These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| blood vessels Fang, Yi-Ru verfasserin aut Wato, Masahiro verfasserin aut Hata, Shintaro oth Tanaka, Akio oth In Journal of oral pathology & medicine Oxford [u.a.] : Wiley-Blackwell, 1972 34(2005), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927231 (DE-600)2026385-5 1600-0714 nnns volume:34 year:2005 number:6 pages:0 http://dx.doi.org/10.1111/j.1600-0714.2005.00316.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 34 2005 6 0 |
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immunohistochemical co-localization of lymphatics and blood vessels in oral squamous cell carcinomas |
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Immunohistochemical co-localization of lymphatics and blood vessels in oral squamous cell carcinomas |
abstract |
Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.Methods: Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with 5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′-diaminobenzidine (DAB) as color reagents, respectively.Results: In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.Conclusions: These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral. |
abstractGer |
Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.Methods: Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with 5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′-diaminobenzidine (DAB) as color reagents, respectively.Results: In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.Conclusions: These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral. |
abstract_unstemmed |
Background: Differentiating lymphatic vessels from blood vessels is difficult, partly due to the lack of a specific method for identifying lymphatics. A new lymphatic vessel-reactive antibody, D2-40 has recently become commercially available. We examined the selectivity of D2-40 for lymphatics in oral neoplastic lesions for discrimination from blood vessels.Methods: Formalin-fixed, paraffin-embedded sections of oral lymphangiomas (n = 3), oral hemangiomas (n = 7), and oral squamous cell carcinomas (OSCC, n = 46) were double immunostained with D2-40 and anti-CD34 monoclonal antibodies (MoAb) using ENVISION-polymer technique with 5-bromo-4-chloro-3-indoxyl-phosphate (BCIP)/nitroblue tetrazolium chloride (NBT) and 3,3′-diaminobenzidine (DAB) as color reagents, respectively.Results: In the oral lymphangiomas and hemangiomas D2-40 was detected in all lymphatics, while all blood vessels were positive for CD34. In OSCC, number of vessels for lymphatics (P < 0.01) and for blood vessels in the perineoplastic areas were significantly greater than those in intratumoral areas.Conclusions: These results indicate that lymphatic proliferation might be much more extensive in the peritumoral area than intratumoral. |
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title_short |
Immunohistochemical co-localization of lymphatics and blood vessels in oral squamous cell carcinomas |
url |
http://dx.doi.org/10.1111/j.1600-0714.2005.00316.x |
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author2 |
Fang, Yi-Ru Wato, Masahiro Hata, Shintaro Tanaka, Akio |
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Fang, Yi-Ru Wato, Masahiro Hata, Shintaro Tanaka, Akio |
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10.1111/j.1600-0714.2005.00316.x |
up_date |
2024-07-06T04:02:45.479Z |
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