Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts
Abstract: The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1β (IL-1β), was studied. PHT (20 μg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergis...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Modéer, T. [verfasserIn] Domeij, H. [verfasserIn] Andurén, I. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Copenhagen: Munksgaard International Publishers ; 2000 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2002 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Journal of oral pathology & medicine - Oxford [u.a.] : Wiley-Blackwell, 1972, 29(2000), 10, Seite 0 |
| Übergeordnetes Werk: |
volume:29 ; year:2000 ; number:10 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1034/j.1600-0714.2000.291003.x |
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NLEJ243038615 |
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| 520 | |a Abstract: The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1β (IL-1β), was studied. PHT (20 μg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1β (30 pg/ml). The stimulatory effect of PHT on IL-1β-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 μM). The anti-inflammatory drug, dexamethasone (1 μM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1β. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth. | ||
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10.1034/j.1600-0714.2000.291003.x doi (DE-627)NLEJ243038615 DE-627 ger DE-627 rakwb Modéer, T. verfasserin aut Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts Copenhagen Munksgaard International Publishers 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1β (IL-1β), was studied. PHT (20 μg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1β (30 pg/ml). The stimulatory effect of PHT on IL-1β-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 μM). The anti-inflammatory drug, dexamethasone (1 μM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1β. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| gingival fibroblasts Domeij, H. verfasserin aut Andurén, I. verfasserin aut Mustafa, M. oth Brunius, G. oth In Journal of oral pathology & medicine Oxford [u.a.] : Wiley-Blackwell, 1972 29(2000), 10, Seite 0 Online-Ressource (DE-627)NLEJ243927231 (DE-600)2026385-5 1600-0714 nnns volume:29 year:2000 number:10 pages:0 http://dx.doi.org/10.1034/j.1600-0714.2000.291003.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 2000 10 0 |
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10.1034/j.1600-0714.2000.291003.x doi (DE-627)NLEJ243038615 DE-627 ger DE-627 rakwb Modéer, T. verfasserin aut Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts Copenhagen Munksgaard International Publishers 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1β (IL-1β), was studied. PHT (20 μg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1β (30 pg/ml). The stimulatory effect of PHT on IL-1β-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 μM). The anti-inflammatory drug, dexamethasone (1 μM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1β. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| gingival fibroblasts Domeij, H. verfasserin aut Andurén, I. verfasserin aut Mustafa, M. oth Brunius, G. oth In Journal of oral pathology & medicine Oxford [u.a.] : Wiley-Blackwell, 1972 29(2000), 10, Seite 0 Online-Ressource (DE-627)NLEJ243927231 (DE-600)2026385-5 1600-0714 nnns volume:29 year:2000 number:10 pages:0 http://dx.doi.org/10.1034/j.1600-0714.2000.291003.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 2000 10 0 |
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10.1034/j.1600-0714.2000.291003.x doi (DE-627)NLEJ243038615 DE-627 ger DE-627 rakwb Modéer, T. verfasserin aut Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts Copenhagen Munksgaard International Publishers 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1β (IL-1β), was studied. PHT (20 μg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1β (30 pg/ml). The stimulatory effect of PHT on IL-1β-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 μM). The anti-inflammatory drug, dexamethasone (1 μM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1β. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| gingival fibroblasts Domeij, H. verfasserin aut Andurén, I. verfasserin aut Mustafa, M. oth Brunius, G. oth In Journal of oral pathology & medicine Oxford [u.a.] : Wiley-Blackwell, 1972 29(2000), 10, Seite 0 Online-Ressource (DE-627)NLEJ243927231 (DE-600)2026385-5 1600-0714 nnns volume:29 year:2000 number:10 pages:0 http://dx.doi.org/10.1034/j.1600-0714.2000.291003.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 2000 10 0 |
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10.1034/j.1600-0714.2000.291003.x doi (DE-627)NLEJ243038615 DE-627 ger DE-627 rakwb Modéer, T. verfasserin aut Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts Copenhagen Munksgaard International Publishers 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1β (IL-1β), was studied. PHT (20 μg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1β (30 pg/ml). The stimulatory effect of PHT on IL-1β-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 μM). The anti-inflammatory drug, dexamethasone (1 μM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1β. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| gingival fibroblasts Domeij, H. verfasserin aut Andurén, I. verfasserin aut Mustafa, M. oth Brunius, G. oth In Journal of oral pathology & medicine Oxford [u.a.] : Wiley-Blackwell, 1972 29(2000), 10, Seite 0 Online-Ressource (DE-627)NLEJ243927231 (DE-600)2026385-5 1600-0714 nnns volume:29 year:2000 number:10 pages:0 http://dx.doi.org/10.1034/j.1600-0714.2000.291003.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 2000 10 0 |
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10.1034/j.1600-0714.2000.291003.x doi (DE-627)NLEJ243038615 DE-627 ger DE-627 rakwb Modéer, T. verfasserin aut Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts Copenhagen Munksgaard International Publishers 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1β (IL-1β), was studied. PHT (20 μg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1β (30 pg/ml). The stimulatory effect of PHT on IL-1β-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 μM). The anti-inflammatory drug, dexamethasone (1 μM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1β. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| gingival fibroblasts Domeij, H. verfasserin aut Andurén, I. verfasserin aut Mustafa, M. oth Brunius, G. oth In Journal of oral pathology & medicine Oxford [u.a.] : Wiley-Blackwell, 1972 29(2000), 10, Seite 0 Online-Ressource (DE-627)NLEJ243927231 (DE-600)2026385-5 1600-0714 nnns volume:29 year:2000 number:10 pages:0 http://dx.doi.org/10.1034/j.1600-0714.2000.291003.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 29 2000 10 0 |
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Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts |
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Abstract: The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1β (IL-1β), was studied. PHT (20 μg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1β (30 pg/ml). The stimulatory effect of PHT on IL-1β-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 μM). The anti-inflammatory drug, dexamethasone (1 μM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1β. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth. |
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Abstract: The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1β (IL-1β), was studied. PHT (20 μg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1β (30 pg/ml). The stimulatory effect of PHT on IL-1β-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 μM). The anti-inflammatory drug, dexamethasone (1 μM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1β. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth. |
| abstract_unstemmed |
Abstract: The in vitro effect of phenytoin (PHT) on the production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in human gingival fibroblasts, challenged with or without interleukin-1β (IL-1β), was studied. PHT (20 μg/ml) alone increased the mRNA level for both IL-6 and IL-8, as well as synergistically enhancing the production of IL-6 and IL-8, at both transcriptional and translational level in fibroblasts challenged with IL-1β (30 pg/ml). The stimulatory effect of PHT on IL-1β-induced IL-6 production was strongly reduced by the specific cyclooxygenase-2 inhibitor NS-398 (1 μM). The anti-inflammatory drug, dexamethasone (1 μM), abolished the production of both IL-6 and IL-8 in gingival fibroblasts challenged with PHT in the presence or absence of IL-1β. The ability of PHT, alone as well as in combination with IL-1, to upregulate the production of IL-6 and IL-8 in human gingival fibroblasts may contribute to enhanced recruitment and activation of inflammatory cells. This effect of PHT may thereby give a prerequisite for the establishment of an interaction between cytokines and connective tissue cells in the periodontal tissue, which is suggested to lead to gingival overgrowth. |
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Effect of phenytoin on the production of interleukin-6 and interleukin-8 in human gingival fibroblasts |
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