Determination of the Phospholipid Precursor of Anandamide and Other N-Acylethanolamine Phospholipids Before and After Sodium Azide-Induced Toxicity in Cultured Neocortical Neurons
Phospholipase d-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subject...
Ausführliche Beschreibung
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| Autor*in: |
Hansen, Henrik H [verfasserIn] Hansen, Steen Honoré [verfasserIn] Schousboe, Arne [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford UK: Blackwell Science Ltd. ; 2000 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2002 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Journal of neurochemistry - Oxford : Wiley-Blackwell, 1956, 75(2000), 2, Seite 0 |
| Übergeordnetes Werk: |
volume:75 ; year:2000 ; number:2 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1046/j.1471-4159.2000.0750861.x |
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| 245 | 1 | 0 | |a Determination of the Phospholipid Precursor of Anandamide and Other N-Acylethanolamine Phospholipids Before and After Sodium Azide-Induced Toxicity in Cultured Neocortical Neurons |
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| 520 | |a Phospholipase d-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subjected to sodium azide-induced cell injury. We here extend the information on the NAPE response, reporting on the composition of N-acylspecies of NAPE, employing a new methodological approach of HPLC-coupled electrospray ionization mass spectrometry. Exposure to sodium azide (5 mM) increased the total amount of NAPE threefold over control levels ; however, no alteration of the relative composition of NAPE species was detected. The anandamide precursor (20 : 4-NAPE) constituted only 0.1% of all NAPEs detected in the neurons. Total NAPE species in control cells amounted to 956-1,060 pmol/107 cells. Moreover, we detected the presence of an unknown NAPE species with molecular weight identical to 20 : 4-NAPE. This may suggest the presence of a putative stereoisomer of the anandamide precursor with at least one trans-configured double bond in the N-arachidonoyl moiety. These results show that with the present method, neuronal NAPE species can be identified and quantified with respect to N-acyl composition, including a trans-isomer of the anandamide precursor. The anandamide precursor is up-regulated to the same extent as other NAPEs upon neuronal injury. | ||
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10.1046/j.1471-4159.2000.0750861.x doi (DE-627)NLEJ243148992 DE-627 ger DE-627 rakwb Hansen, Henrik H verfasserin aut Determination of the Phospholipid Precursor of Anandamide and Other N-Acylethanolamine Phospholipids Before and After Sodium Azide-Induced Toxicity in Cultured Neocortical Neurons Oxford UK Blackwell Science Ltd. 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Phospholipase d-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subjected to sodium azide-induced cell injury. We here extend the information on the NAPE response, reporting on the composition of N-acylspecies of NAPE, employing a new methodological approach of HPLC-coupled electrospray ionization mass spectrometry. Exposure to sodium azide (5 mM) increased the total amount of NAPE threefold over control levels ; however, no alteration of the relative composition of NAPE species was detected. The anandamide precursor (20 : 4-NAPE) constituted only 0.1% of all NAPEs detected in the neurons. Total NAPE species in control cells amounted to 956-1,060 pmol/107 cells. Moreover, we detected the presence of an unknown NAPE species with molecular weight identical to 20 : 4-NAPE. This may suggest the presence of a putative stereoisomer of the anandamide precursor with at least one trans-configured double bond in the N-arachidonoyl moiety. These results show that with the present method, neuronal NAPE species can be identified and quantified with respect to N-acyl composition, including a trans-isomer of the anandamide precursor. The anandamide precursor is up-regulated to the same extent as other NAPEs upon neuronal injury. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Anandamide Hansen, Steen Honoré verfasserin aut Schousboe, Arne verfasserin aut Hansen, Harald S oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 75(2000), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:75 year:2000 number:2 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.0750861.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 75 2000 2 0 |
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10.1046/j.1471-4159.2000.0750861.x doi (DE-627)NLEJ243148992 DE-627 ger DE-627 rakwb Hansen, Henrik H verfasserin aut Determination of the Phospholipid Precursor of Anandamide and Other N-Acylethanolamine Phospholipids Before and After Sodium Azide-Induced Toxicity in Cultured Neocortical Neurons Oxford UK Blackwell Science Ltd. 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Phospholipase d-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subjected to sodium azide-induced cell injury. We here extend the information on the NAPE response, reporting on the composition of N-acylspecies of NAPE, employing a new methodological approach of HPLC-coupled electrospray ionization mass spectrometry. Exposure to sodium azide (5 mM) increased the total amount of NAPE threefold over control levels ; however, no alteration of the relative composition of NAPE species was detected. The anandamide precursor (20 : 4-NAPE) constituted only 0.1% of all NAPEs detected in the neurons. Total NAPE species in control cells amounted to 956-1,060 pmol/107 cells. Moreover, we detected the presence of an unknown NAPE species with molecular weight identical to 20 : 4-NAPE. This may suggest the presence of a putative stereoisomer of the anandamide precursor with at least one trans-configured double bond in the N-arachidonoyl moiety. These results show that with the present method, neuronal NAPE species can be identified and quantified with respect to N-acyl composition, including a trans-isomer of the anandamide precursor. The anandamide precursor is up-regulated to the same extent as other NAPEs upon neuronal injury. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Anandamide Hansen, Steen Honoré verfasserin aut Schousboe, Arne verfasserin aut Hansen, Harald S oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 75(2000), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:75 year:2000 number:2 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.0750861.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 75 2000 2 0 |
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10.1046/j.1471-4159.2000.0750861.x doi (DE-627)NLEJ243148992 DE-627 ger DE-627 rakwb Hansen, Henrik H verfasserin aut Determination of the Phospholipid Precursor of Anandamide and Other N-Acylethanolamine Phospholipids Before and After Sodium Azide-Induced Toxicity in Cultured Neocortical Neurons Oxford UK Blackwell Science Ltd. 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Phospholipase d-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subjected to sodium azide-induced cell injury. We here extend the information on the NAPE response, reporting on the composition of N-acylspecies of NAPE, employing a new methodological approach of HPLC-coupled electrospray ionization mass spectrometry. Exposure to sodium azide (5 mM) increased the total amount of NAPE threefold over control levels ; however, no alteration of the relative composition of NAPE species was detected. The anandamide precursor (20 : 4-NAPE) constituted only 0.1% of all NAPEs detected in the neurons. Total NAPE species in control cells amounted to 956-1,060 pmol/107 cells. Moreover, we detected the presence of an unknown NAPE species with molecular weight identical to 20 : 4-NAPE. This may suggest the presence of a putative stereoisomer of the anandamide precursor with at least one trans-configured double bond in the N-arachidonoyl moiety. These results show that with the present method, neuronal NAPE species can be identified and quantified with respect to N-acyl composition, including a trans-isomer of the anandamide precursor. The anandamide precursor is up-regulated to the same extent as other NAPEs upon neuronal injury. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Anandamide Hansen, Steen Honoré verfasserin aut Schousboe, Arne verfasserin aut Hansen, Harald S oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 75(2000), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:75 year:2000 number:2 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.0750861.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 75 2000 2 0 |
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10.1046/j.1471-4159.2000.0750861.x doi (DE-627)NLEJ243148992 DE-627 ger DE-627 rakwb Hansen, Henrik H verfasserin aut Determination of the Phospholipid Precursor of Anandamide and Other N-Acylethanolamine Phospholipids Before and After Sodium Azide-Induced Toxicity in Cultured Neocortical Neurons Oxford UK Blackwell Science Ltd. 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Phospholipase d-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subjected to sodium azide-induced cell injury. We here extend the information on the NAPE response, reporting on the composition of N-acylspecies of NAPE, employing a new methodological approach of HPLC-coupled electrospray ionization mass spectrometry. Exposure to sodium azide (5 mM) increased the total amount of NAPE threefold over control levels ; however, no alteration of the relative composition of NAPE species was detected. The anandamide precursor (20 : 4-NAPE) constituted only 0.1% of all NAPEs detected in the neurons. Total NAPE species in control cells amounted to 956-1,060 pmol/107 cells. Moreover, we detected the presence of an unknown NAPE species with molecular weight identical to 20 : 4-NAPE. This may suggest the presence of a putative stereoisomer of the anandamide precursor with at least one trans-configured double bond in the N-arachidonoyl moiety. These results show that with the present method, neuronal NAPE species can be identified and quantified with respect to N-acyl composition, including a trans-isomer of the anandamide precursor. The anandamide precursor is up-regulated to the same extent as other NAPEs upon neuronal injury. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Anandamide Hansen, Steen Honoré verfasserin aut Schousboe, Arne verfasserin aut Hansen, Harald S oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 75(2000), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:75 year:2000 number:2 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.0750861.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 75 2000 2 0 |
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10.1046/j.1471-4159.2000.0750861.x doi (DE-627)NLEJ243148992 DE-627 ger DE-627 rakwb Hansen, Henrik H verfasserin aut Determination of the Phospholipid Precursor of Anandamide and Other N-Acylethanolamine Phospholipids Before and After Sodium Azide-Induced Toxicity in Cultured Neocortical Neurons Oxford UK Blackwell Science Ltd. 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Phospholipase d-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subjected to sodium azide-induced cell injury. We here extend the information on the NAPE response, reporting on the composition of N-acylspecies of NAPE, employing a new methodological approach of HPLC-coupled electrospray ionization mass spectrometry. Exposure to sodium azide (5 mM) increased the total amount of NAPE threefold over control levels ; however, no alteration of the relative composition of NAPE species was detected. The anandamide precursor (20 : 4-NAPE) constituted only 0.1% of all NAPEs detected in the neurons. Total NAPE species in control cells amounted to 956-1,060 pmol/107 cells. Moreover, we detected the presence of an unknown NAPE species with molecular weight identical to 20 : 4-NAPE. This may suggest the presence of a putative stereoisomer of the anandamide precursor with at least one trans-configured double bond in the N-arachidonoyl moiety. These results show that with the present method, neuronal NAPE species can be identified and quantified with respect to N-acyl composition, including a trans-isomer of the anandamide precursor. The anandamide precursor is up-regulated to the same extent as other NAPEs upon neuronal injury. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Anandamide Hansen, Steen Honoré verfasserin aut Schousboe, Arne verfasserin aut Hansen, Harald S oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 75(2000), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:75 year:2000 number:2 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.0750861.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 75 2000 2 0 |
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determination of the phospholipid precursor of anandamide and other n-acylethanolamine phospholipids before and after sodium azide-induced toxicity in cultured neocortical neurons |
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Determination of the Phospholipid Precursor of Anandamide and Other N-Acylethanolamine Phospholipids Before and After Sodium Azide-Induced Toxicity in Cultured Neocortical Neurons |
| abstract |
Phospholipase d-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subjected to sodium azide-induced cell injury. We here extend the information on the NAPE response, reporting on the composition of N-acylspecies of NAPE, employing a new methodological approach of HPLC-coupled electrospray ionization mass spectrometry. Exposure to sodium azide (5 mM) increased the total amount of NAPE threefold over control levels ; however, no alteration of the relative composition of NAPE species was detected. The anandamide precursor (20 : 4-NAPE) constituted only 0.1% of all NAPEs detected in the neurons. Total NAPE species in control cells amounted to 956-1,060 pmol/107 cells. Moreover, we detected the presence of an unknown NAPE species with molecular weight identical to 20 : 4-NAPE. This may suggest the presence of a putative stereoisomer of the anandamide precursor with at least one trans-configured double bond in the N-arachidonoyl moiety. These results show that with the present method, neuronal NAPE species can be identified and quantified with respect to N-acyl composition, including a trans-isomer of the anandamide precursor. The anandamide precursor is up-regulated to the same extent as other NAPEs upon neuronal injury. |
| abstractGer |
Phospholipase d-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subjected to sodium azide-induced cell injury. We here extend the information on the NAPE response, reporting on the composition of N-acylspecies of NAPE, employing a new methodological approach of HPLC-coupled electrospray ionization mass spectrometry. Exposure to sodium azide (5 mM) increased the total amount of NAPE threefold over control levels ; however, no alteration of the relative composition of NAPE species was detected. The anandamide precursor (20 : 4-NAPE) constituted only 0.1% of all NAPEs detected in the neurons. Total NAPE species in control cells amounted to 956-1,060 pmol/107 cells. Moreover, we detected the presence of an unknown NAPE species with molecular weight identical to 20 : 4-NAPE. This may suggest the presence of a putative stereoisomer of the anandamide precursor with at least one trans-configured double bond in the N-arachidonoyl moiety. These results show that with the present method, neuronal NAPE species can be identified and quantified with respect to N-acyl composition, including a trans-isomer of the anandamide precursor. The anandamide precursor is up-regulated to the same extent as other NAPEs upon neuronal injury. |
| abstract_unstemmed |
Phospholipase d-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subjected to sodium azide-induced cell injury. We here extend the information on the NAPE response, reporting on the composition of N-acylspecies of NAPE, employing a new methodological approach of HPLC-coupled electrospray ionization mass spectrometry. Exposure to sodium azide (5 mM) increased the total amount of NAPE threefold over control levels ; however, no alteration of the relative composition of NAPE species was detected. The anandamide precursor (20 : 4-NAPE) constituted only 0.1% of all NAPEs detected in the neurons. Total NAPE species in control cells amounted to 956-1,060 pmol/107 cells. Moreover, we detected the presence of an unknown NAPE species with molecular weight identical to 20 : 4-NAPE. This may suggest the presence of a putative stereoisomer of the anandamide precursor with at least one trans-configured double bond in the N-arachidonoyl moiety. These results show that with the present method, neuronal NAPE species can be identified and quantified with respect to N-acyl composition, including a trans-isomer of the anandamide precursor. The anandamide precursor is up-regulated to the same extent as other NAPEs upon neuronal injury. |
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| title_short |
Determination of the Phospholipid Precursor of Anandamide and Other N-Acylethanolamine Phospholipids Before and After Sodium Azide-Induced Toxicity in Cultured Neocortical Neurons |
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http://dx.doi.org/10.1046/j.1471-4159.2000.0750861.x |
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| author2 |
Hansen, Steen Honoré Schousboe, Arne Hansen, Harald S |
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Hansen, Steen Honoré Schousboe, Arne Hansen, Harald S |
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| doi_str |
10.1046/j.1471-4159.2000.0750861.x |
| up_date |
2024-07-06T04:28:29.154Z |
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