Molecular Cloning of a Novel Brain-Type Na+-Dependent Inorganic Phosphate Cotransporter
Abstract: We have isolated a human cDNA encoding a protein, designated DNPI, that shows 82% amino acid identity and 92% similarity to the human brain-specific Na+-dependent inorganic phosphate (Na+/Pi) cotransporter (BNPI), which is localized exclusively to neuron-rich regions. Expression of DNPI mR...
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Oxford UK: Blackwell Science Ltd ; 2000 |
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Online-Ressource |
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2002 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: Journal of neurochemistry - Oxford : Wiley-Blackwell, 1956, 74(2000), 6, Seite 0 |
| Übergeordnetes Werk: |
volume:74 ; year:2000 ; number:6 ; pages:0 |
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| DOI / URN: |
10.1046/j.1471-4159.2000.0742622.x |
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| 520 | |a Abstract: We have isolated a human cDNA encoding a protein, designated DNPI, that shows 82% amino acid identity and 92% similarity to the human brain-specific Na+-dependent inorganic phosphate (Na+/Pi) cotransporter (BNPI), which is localized exclusively to neuron-rich regions. Expression of DNPI mRNA in Xenopus oocytes resulted in a significant increase in Na+-dependent Pi transport, indicating that DNPI is a novel Na+/Pi cotransporter. Northern blot analysis shows that DNPI mRNA is expressed predominantly in brain, where the highest levels are observed in medulla, substantia nigra, subthalamic nucleus, and thalamus, all of which express BNPI mRNA at low levels. In contrast, DNPI mRNA is expressed at low levels in cerebellum and hippocampus, where BNPI mRNA is expressed at high levels. No hybridizing signal for DNPI mRNA is observed in the glia-rich region of corpus callosum. In other regions examined, both mRNAs are moderately or highly expressed. These results indicate that BNPI and DNPI, which coordinate Na+-dependent Pi transport in the neuron-rich regions of the brain, may form a new class within the Na+/Pi cotransporter family. | ||
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| 700 | 1 | |a Onda, Hideaki |4 oth | |
| 700 | 1 | |a Hisano, Setsuji |4 oth | |
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| 700 | 1 | |a Hori, Tomokatsu |4 oth | |
| 700 | 1 | |a Yamada, Shirou |4 oth | |
| 700 | 1 | |a Tomura, Hideaki |4 oth | |
| 700 | 1 | |a Yamada, Yuichiro |4 oth | |
| 700 | 1 | |a Inoue, Ituro |4 oth | |
| 700 | 1 | |a Kojima, Itaru |4 oth | |
| 700 | 1 | |a Takeda, Jun |4 oth | |
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10.1046/j.1471-4159.2000.0742622.x doi (DE-627)NLEJ243149980 DE-627 ger DE-627 rakwb Molecular Cloning of a Novel Brain-Type Na+-Dependent Inorganic Phosphate Cotransporter Oxford UK Blackwell Science Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: We have isolated a human cDNA encoding a protein, designated DNPI, that shows 82% amino acid identity and 92% similarity to the human brain-specific Na+-dependent inorganic phosphate (Na+/Pi) cotransporter (BNPI), which is localized exclusively to neuron-rich regions. Expression of DNPI mRNA in Xenopus oocytes resulted in a significant increase in Na+-dependent Pi transport, indicating that DNPI is a novel Na+/Pi cotransporter. Northern blot analysis shows that DNPI mRNA is expressed predominantly in brain, where the highest levels are observed in medulla, substantia nigra, subthalamic nucleus, and thalamus, all of which express BNPI mRNA at low levels. In contrast, DNPI mRNA is expressed at low levels in cerebellum and hippocampus, where BNPI mRNA is expressed at high levels. No hybridizing signal for DNPI mRNA is observed in the glia-rich region of corpus callosum. In other regions examined, both mRNAs are moderately or highly expressed. These results indicate that BNPI and DNPI, which coordinate Na+-dependent Pi transport in the neuron-rich regions of the brain, may form a new class within the Na+/Pi cotransporter family. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| cDNA cloning Aihara, Yasuo oth Mashima, Hirosato oth Onda, Hideaki oth Hisano, Setsuji oth Kasuya, Hidetoshi oth Hori, Tomokatsu oth Yamada, Shirou oth Tomura, Hideaki oth Yamada, Yuichiro oth Inoue, Ituro oth Kojima, Itaru oth Takeda, Jun oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 74(2000), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:74 year:2000 number:6 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.0742622.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 74 2000 6 0 |
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10.1046/j.1471-4159.2000.0742622.x doi (DE-627)NLEJ243149980 DE-627 ger DE-627 rakwb Molecular Cloning of a Novel Brain-Type Na+-Dependent Inorganic Phosphate Cotransporter Oxford UK Blackwell Science Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: We have isolated a human cDNA encoding a protein, designated DNPI, that shows 82% amino acid identity and 92% similarity to the human brain-specific Na+-dependent inorganic phosphate (Na+/Pi) cotransporter (BNPI), which is localized exclusively to neuron-rich regions. Expression of DNPI mRNA in Xenopus oocytes resulted in a significant increase in Na+-dependent Pi transport, indicating that DNPI is a novel Na+/Pi cotransporter. Northern blot analysis shows that DNPI mRNA is expressed predominantly in brain, where the highest levels are observed in medulla, substantia nigra, subthalamic nucleus, and thalamus, all of which express BNPI mRNA at low levels. In contrast, DNPI mRNA is expressed at low levels in cerebellum and hippocampus, where BNPI mRNA is expressed at high levels. No hybridizing signal for DNPI mRNA is observed in the glia-rich region of corpus callosum. In other regions examined, both mRNAs are moderately or highly expressed. These results indicate that BNPI and DNPI, which coordinate Na+-dependent Pi transport in the neuron-rich regions of the brain, may form a new class within the Na+/Pi cotransporter family. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| cDNA cloning Aihara, Yasuo oth Mashima, Hirosato oth Onda, Hideaki oth Hisano, Setsuji oth Kasuya, Hidetoshi oth Hori, Tomokatsu oth Yamada, Shirou oth Tomura, Hideaki oth Yamada, Yuichiro oth Inoue, Ituro oth Kojima, Itaru oth Takeda, Jun oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 74(2000), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:74 year:2000 number:6 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.0742622.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 74 2000 6 0 |
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10.1046/j.1471-4159.2000.0742622.x doi (DE-627)NLEJ243149980 DE-627 ger DE-627 rakwb Molecular Cloning of a Novel Brain-Type Na+-Dependent Inorganic Phosphate Cotransporter Oxford UK Blackwell Science Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: We have isolated a human cDNA encoding a protein, designated DNPI, that shows 82% amino acid identity and 92% similarity to the human brain-specific Na+-dependent inorganic phosphate (Na+/Pi) cotransporter (BNPI), which is localized exclusively to neuron-rich regions. Expression of DNPI mRNA in Xenopus oocytes resulted in a significant increase in Na+-dependent Pi transport, indicating that DNPI is a novel Na+/Pi cotransporter. Northern blot analysis shows that DNPI mRNA is expressed predominantly in brain, where the highest levels are observed in medulla, substantia nigra, subthalamic nucleus, and thalamus, all of which express BNPI mRNA at low levels. In contrast, DNPI mRNA is expressed at low levels in cerebellum and hippocampus, where BNPI mRNA is expressed at high levels. No hybridizing signal for DNPI mRNA is observed in the glia-rich region of corpus callosum. In other regions examined, both mRNAs are moderately or highly expressed. These results indicate that BNPI and DNPI, which coordinate Na+-dependent Pi transport in the neuron-rich regions of the brain, may form a new class within the Na+/Pi cotransporter family. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| cDNA cloning Aihara, Yasuo oth Mashima, Hirosato oth Onda, Hideaki oth Hisano, Setsuji oth Kasuya, Hidetoshi oth Hori, Tomokatsu oth Yamada, Shirou oth Tomura, Hideaki oth Yamada, Yuichiro oth Inoue, Ituro oth Kojima, Itaru oth Takeda, Jun oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 74(2000), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:74 year:2000 number:6 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.0742622.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 74 2000 6 0 |
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10.1046/j.1471-4159.2000.0742622.x doi (DE-627)NLEJ243149980 DE-627 ger DE-627 rakwb Molecular Cloning of a Novel Brain-Type Na+-Dependent Inorganic Phosphate Cotransporter Oxford UK Blackwell Science Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: We have isolated a human cDNA encoding a protein, designated DNPI, that shows 82% amino acid identity and 92% similarity to the human brain-specific Na+-dependent inorganic phosphate (Na+/Pi) cotransporter (BNPI), which is localized exclusively to neuron-rich regions. Expression of DNPI mRNA in Xenopus oocytes resulted in a significant increase in Na+-dependent Pi transport, indicating that DNPI is a novel Na+/Pi cotransporter. Northern blot analysis shows that DNPI mRNA is expressed predominantly in brain, where the highest levels are observed in medulla, substantia nigra, subthalamic nucleus, and thalamus, all of which express BNPI mRNA at low levels. In contrast, DNPI mRNA is expressed at low levels in cerebellum and hippocampus, where BNPI mRNA is expressed at high levels. No hybridizing signal for DNPI mRNA is observed in the glia-rich region of corpus callosum. In other regions examined, both mRNAs are moderately or highly expressed. These results indicate that BNPI and DNPI, which coordinate Na+-dependent Pi transport in the neuron-rich regions of the brain, may form a new class within the Na+/Pi cotransporter family. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| cDNA cloning Aihara, Yasuo oth Mashima, Hirosato oth Onda, Hideaki oth Hisano, Setsuji oth Kasuya, Hidetoshi oth Hori, Tomokatsu oth Yamada, Shirou oth Tomura, Hideaki oth Yamada, Yuichiro oth Inoue, Ituro oth Kojima, Itaru oth Takeda, Jun oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 74(2000), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:74 year:2000 number:6 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.0742622.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 74 2000 6 0 |
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10.1046/j.1471-4159.2000.0742622.x doi (DE-627)NLEJ243149980 DE-627 ger DE-627 rakwb Molecular Cloning of a Novel Brain-Type Na+-Dependent Inorganic Phosphate Cotransporter Oxford UK Blackwell Science Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: We have isolated a human cDNA encoding a protein, designated DNPI, that shows 82% amino acid identity and 92% similarity to the human brain-specific Na+-dependent inorganic phosphate (Na+/Pi) cotransporter (BNPI), which is localized exclusively to neuron-rich regions. Expression of DNPI mRNA in Xenopus oocytes resulted in a significant increase in Na+-dependent Pi transport, indicating that DNPI is a novel Na+/Pi cotransporter. Northern blot analysis shows that DNPI mRNA is expressed predominantly in brain, where the highest levels are observed in medulla, substantia nigra, subthalamic nucleus, and thalamus, all of which express BNPI mRNA at low levels. In contrast, DNPI mRNA is expressed at low levels in cerebellum and hippocampus, where BNPI mRNA is expressed at high levels. No hybridizing signal for DNPI mRNA is observed in the glia-rich region of corpus callosum. In other regions examined, both mRNAs are moderately or highly expressed. These results indicate that BNPI and DNPI, which coordinate Na+-dependent Pi transport in the neuron-rich regions of the brain, may form a new class within the Na+/Pi cotransporter family. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| cDNA cloning Aihara, Yasuo oth Mashima, Hirosato oth Onda, Hideaki oth Hisano, Setsuji oth Kasuya, Hidetoshi oth Hori, Tomokatsu oth Yamada, Shirou oth Tomura, Hideaki oth Yamada, Yuichiro oth Inoue, Ituro oth Kojima, Itaru oth Takeda, Jun oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 74(2000), 6, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:74 year:2000 number:6 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.0742622.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 74 2000 6 0 |
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molecular cloning of a novel brain-type na+-dependent inorganic phosphate cotransporter |
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Molecular Cloning of a Novel Brain-Type Na+-Dependent Inorganic Phosphate Cotransporter |
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Abstract: We have isolated a human cDNA encoding a protein, designated DNPI, that shows 82% amino acid identity and 92% similarity to the human brain-specific Na+-dependent inorganic phosphate (Na+/Pi) cotransporter (BNPI), which is localized exclusively to neuron-rich regions. Expression of DNPI mRNA in Xenopus oocytes resulted in a significant increase in Na+-dependent Pi transport, indicating that DNPI is a novel Na+/Pi cotransporter. Northern blot analysis shows that DNPI mRNA is expressed predominantly in brain, where the highest levels are observed in medulla, substantia nigra, subthalamic nucleus, and thalamus, all of which express BNPI mRNA at low levels. In contrast, DNPI mRNA is expressed at low levels in cerebellum and hippocampus, where BNPI mRNA is expressed at high levels. No hybridizing signal for DNPI mRNA is observed in the glia-rich region of corpus callosum. In other regions examined, both mRNAs are moderately or highly expressed. These results indicate that BNPI and DNPI, which coordinate Na+-dependent Pi transport in the neuron-rich regions of the brain, may form a new class within the Na+/Pi cotransporter family. |
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Abstract: We have isolated a human cDNA encoding a protein, designated DNPI, that shows 82% amino acid identity and 92% similarity to the human brain-specific Na+-dependent inorganic phosphate (Na+/Pi) cotransporter (BNPI), which is localized exclusively to neuron-rich regions. Expression of DNPI mRNA in Xenopus oocytes resulted in a significant increase in Na+-dependent Pi transport, indicating that DNPI is a novel Na+/Pi cotransporter. Northern blot analysis shows that DNPI mRNA is expressed predominantly in brain, where the highest levels are observed in medulla, substantia nigra, subthalamic nucleus, and thalamus, all of which express BNPI mRNA at low levels. In contrast, DNPI mRNA is expressed at low levels in cerebellum and hippocampus, where BNPI mRNA is expressed at high levels. No hybridizing signal for DNPI mRNA is observed in the glia-rich region of corpus callosum. In other regions examined, both mRNAs are moderately or highly expressed. These results indicate that BNPI and DNPI, which coordinate Na+-dependent Pi transport in the neuron-rich regions of the brain, may form a new class within the Na+/Pi cotransporter family. |
| abstract_unstemmed |
Abstract: We have isolated a human cDNA encoding a protein, designated DNPI, that shows 82% amino acid identity and 92% similarity to the human brain-specific Na+-dependent inorganic phosphate (Na+/Pi) cotransporter (BNPI), which is localized exclusively to neuron-rich regions. Expression of DNPI mRNA in Xenopus oocytes resulted in a significant increase in Na+-dependent Pi transport, indicating that DNPI is a novel Na+/Pi cotransporter. Northern blot analysis shows that DNPI mRNA is expressed predominantly in brain, where the highest levels are observed in medulla, substantia nigra, subthalamic nucleus, and thalamus, all of which express BNPI mRNA at low levels. In contrast, DNPI mRNA is expressed at low levels in cerebellum and hippocampus, where BNPI mRNA is expressed at high levels. No hybridizing signal for DNPI mRNA is observed in the glia-rich region of corpus callosum. In other regions examined, both mRNAs are moderately or highly expressed. These results indicate that BNPI and DNPI, which coordinate Na+-dependent Pi transport in the neuron-rich regions of the brain, may form a new class within the Na+/Pi cotransporter family. |
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| title_short |
Molecular Cloning of a Novel Brain-Type Na+-Dependent Inorganic Phosphate Cotransporter |
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Aihara, Yasuo Mashima, Hirosato Onda, Hideaki Hisano, Setsuji Kasuya, Hidetoshi Hori, Tomokatsu Yamada, Shirou Tomura, Hideaki Yamada, Yuichiro Inoue, Ituro Kojima, Itaru Takeda, Jun |
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Aihara, Yasuo Mashima, Hirosato Onda, Hideaki Hisano, Setsuji Kasuya, Hidetoshi Hori, Tomokatsu Yamada, Shirou Tomura, Hideaki Yamada, Yuichiro Inoue, Ituro Kojima, Itaru Takeda, Jun |
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