Up-Regulation of Base Excision Repair Activity for 8-Hydroxy-2'-Deoxyguanosine in the Mouse Brain After Forebrain Ischemia-Reperfusion
The repair enzyme 8-oxoguanine glycosylase/apyrimidinic/apurinic lyase (OGG) removes 8-hydroxy-2′-deoxyguanosine (oh8dG) in human cells. Our goal was to examine oh8dG-removing activity in the cell nuclei of male C57BL/6 mouse brains treated with either forebrain ischemia-reperfusion (FbIR) or sham o...
Ausführliche Beschreibung
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| Autor*in: |
Lin, Lie-Huey [verfasserIn] Cao, Shutong [verfasserIn] Yu, Ling [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Science Ltd ; 2000 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2003 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Journal of neurochemistry - Oxford : Wiley-Blackwell, 1956, 74(2000), 3, Seite 0 |
| Übergeordnetes Werk: |
volume:74 ; year:2000 ; number:3 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1046/j.1471-4159.2000.741098.x |
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| 520 | |a The repair enzyme 8-oxoguanine glycosylase/apyrimidinic/apurinic lyase (OGG) removes 8-hydroxy-2′-deoxyguanosine (oh8dG) in human cells. Our goal was to examine oh8dG-removing activity in the cell nuclei of male C57BL/6 mouse brains treated with either forebrain ischemia-reperfusion (FbIR) or sham operations. We found that the OGG activity in nuclear extracts, under the condition in which other nucleases did not destroy the oligodeoxynucleotide duplex, excised oh8dG with the greatest efficiency on the oligodeoxynucleotide duplex containing oh8dG/dC and with less efficiency on the heteroduplex containing oh8dG/dT, oh8dG/dG, or oh8dG/dA. This specificity was the same as for the recombinant type 1 OGG (OGG1) of humans. We observed that the OGG1 peptide and its activity in the mouse brain were significantly increased after 90 min of ischemia and 20-30 min of reperfusion. The increase in the protein level and in the activity of brain OGG1 correlated positively with the elevation of FbIR-induced DNA lesions in an indicator gene (the c-fos gene) of the brain. The data suggest a possibility that the OGG1 protein may excise oh8dG in the mouse brain and that the activity of OGG1 may have a functional role in reducing oxidative gene damage in the brain after FbIR. | ||
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10.1046/j.1471-4159.2000.741098.x doi (DE-627)NLEJ243151225 DE-627 ger DE-627 rakwb Lin, Lie-Huey verfasserin aut Up-Regulation of Base Excision Repair Activity for 8-Hydroxy-2'-Deoxyguanosine in the Mouse Brain After Forebrain Ischemia-Reperfusion Oxford, UK Blackwell Science Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The repair enzyme 8-oxoguanine glycosylase/apyrimidinic/apurinic lyase (OGG) removes 8-hydroxy-2′-deoxyguanosine (oh8dG) in human cells. Our goal was to examine oh8dG-removing activity in the cell nuclei of male C57BL/6 mouse brains treated with either forebrain ischemia-reperfusion (FbIR) or sham operations. We found that the OGG activity in nuclear extracts, under the condition in which other nucleases did not destroy the oligodeoxynucleotide duplex, excised oh8dG with the greatest efficiency on the oligodeoxynucleotide duplex containing oh8dG/dC and with less efficiency on the heteroduplex containing oh8dG/dT, oh8dG/dG, or oh8dG/dA. This specificity was the same as for the recombinant type 1 OGG (OGG1) of humans. We observed that the OGG1 peptide and its activity in the mouse brain were significantly increased after 90 min of ischemia and 20-30 min of reperfusion. The increase in the protein level and in the activity of brain OGG1 correlated positively with the elevation of FbIR-induced DNA lesions in an indicator gene (the c-fos gene) of the brain. The data suggest a possibility that the OGG1 protein may excise oh8dG in the mouse brain and that the activity of OGG1 may have a functional role in reducing oxidative gene damage in the brain after FbIR. 2003 Blackwell Publishing Journal Backfiles 1879-2005 |2003|||||||||| Base excision repair Cao, Shutong verfasserin aut Yu, Ling verfasserin aut Cui, Jiankun oth Hamilton, Winifred J. oth Liu, Philip K. oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 74(2000), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:74 year:2000 number:3 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.741098.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 74 2000 3 0 |
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10.1046/j.1471-4159.2000.741098.x doi (DE-627)NLEJ243151225 DE-627 ger DE-627 rakwb Lin, Lie-Huey verfasserin aut Up-Regulation of Base Excision Repair Activity for 8-Hydroxy-2'-Deoxyguanosine in the Mouse Brain After Forebrain Ischemia-Reperfusion Oxford, UK Blackwell Science Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The repair enzyme 8-oxoguanine glycosylase/apyrimidinic/apurinic lyase (OGG) removes 8-hydroxy-2′-deoxyguanosine (oh8dG) in human cells. Our goal was to examine oh8dG-removing activity in the cell nuclei of male C57BL/6 mouse brains treated with either forebrain ischemia-reperfusion (FbIR) or sham operations. We found that the OGG activity in nuclear extracts, under the condition in which other nucleases did not destroy the oligodeoxynucleotide duplex, excised oh8dG with the greatest efficiency on the oligodeoxynucleotide duplex containing oh8dG/dC and with less efficiency on the heteroduplex containing oh8dG/dT, oh8dG/dG, or oh8dG/dA. This specificity was the same as for the recombinant type 1 OGG (OGG1) of humans. We observed that the OGG1 peptide and its activity in the mouse brain were significantly increased after 90 min of ischemia and 20-30 min of reperfusion. The increase in the protein level and in the activity of brain OGG1 correlated positively with the elevation of FbIR-induced DNA lesions in an indicator gene (the c-fos gene) of the brain. The data suggest a possibility that the OGG1 protein may excise oh8dG in the mouse brain and that the activity of OGG1 may have a functional role in reducing oxidative gene damage in the brain after FbIR. 2003 Blackwell Publishing Journal Backfiles 1879-2005 |2003|||||||||| Base excision repair Cao, Shutong verfasserin aut Yu, Ling verfasserin aut Cui, Jiankun oth Hamilton, Winifred J. oth Liu, Philip K. oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 74(2000), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:74 year:2000 number:3 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.741098.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 74 2000 3 0 |
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10.1046/j.1471-4159.2000.741098.x doi (DE-627)NLEJ243151225 DE-627 ger DE-627 rakwb Lin, Lie-Huey verfasserin aut Up-Regulation of Base Excision Repair Activity for 8-Hydroxy-2'-Deoxyguanosine in the Mouse Brain After Forebrain Ischemia-Reperfusion Oxford, UK Blackwell Science Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The repair enzyme 8-oxoguanine glycosylase/apyrimidinic/apurinic lyase (OGG) removes 8-hydroxy-2′-deoxyguanosine (oh8dG) in human cells. Our goal was to examine oh8dG-removing activity in the cell nuclei of male C57BL/6 mouse brains treated with either forebrain ischemia-reperfusion (FbIR) or sham operations. We found that the OGG activity in nuclear extracts, under the condition in which other nucleases did not destroy the oligodeoxynucleotide duplex, excised oh8dG with the greatest efficiency on the oligodeoxynucleotide duplex containing oh8dG/dC and with less efficiency on the heteroduplex containing oh8dG/dT, oh8dG/dG, or oh8dG/dA. This specificity was the same as for the recombinant type 1 OGG (OGG1) of humans. We observed that the OGG1 peptide and its activity in the mouse brain were significantly increased after 90 min of ischemia and 20-30 min of reperfusion. The increase in the protein level and in the activity of brain OGG1 correlated positively with the elevation of FbIR-induced DNA lesions in an indicator gene (the c-fos gene) of the brain. The data suggest a possibility that the OGG1 protein may excise oh8dG in the mouse brain and that the activity of OGG1 may have a functional role in reducing oxidative gene damage in the brain after FbIR. 2003 Blackwell Publishing Journal Backfiles 1879-2005 |2003|||||||||| Base excision repair Cao, Shutong verfasserin aut Yu, Ling verfasserin aut Cui, Jiankun oth Hamilton, Winifred J. oth Liu, Philip K. oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 74(2000), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:74 year:2000 number:3 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.741098.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 74 2000 3 0 |
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10.1046/j.1471-4159.2000.741098.x doi (DE-627)NLEJ243151225 DE-627 ger DE-627 rakwb Lin, Lie-Huey verfasserin aut Up-Regulation of Base Excision Repair Activity for 8-Hydroxy-2'-Deoxyguanosine in the Mouse Brain After Forebrain Ischemia-Reperfusion Oxford, UK Blackwell Science Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The repair enzyme 8-oxoguanine glycosylase/apyrimidinic/apurinic lyase (OGG) removes 8-hydroxy-2′-deoxyguanosine (oh8dG) in human cells. Our goal was to examine oh8dG-removing activity in the cell nuclei of male C57BL/6 mouse brains treated with either forebrain ischemia-reperfusion (FbIR) or sham operations. We found that the OGG activity in nuclear extracts, under the condition in which other nucleases did not destroy the oligodeoxynucleotide duplex, excised oh8dG with the greatest efficiency on the oligodeoxynucleotide duplex containing oh8dG/dC and with less efficiency on the heteroduplex containing oh8dG/dT, oh8dG/dG, or oh8dG/dA. This specificity was the same as for the recombinant type 1 OGG (OGG1) of humans. We observed that the OGG1 peptide and its activity in the mouse brain were significantly increased after 90 min of ischemia and 20-30 min of reperfusion. The increase in the protein level and in the activity of brain OGG1 correlated positively with the elevation of FbIR-induced DNA lesions in an indicator gene (the c-fos gene) of the brain. The data suggest a possibility that the OGG1 protein may excise oh8dG in the mouse brain and that the activity of OGG1 may have a functional role in reducing oxidative gene damage in the brain after FbIR. 2003 Blackwell Publishing Journal Backfiles 1879-2005 |2003|||||||||| Base excision repair Cao, Shutong verfasserin aut Yu, Ling verfasserin aut Cui, Jiankun oth Hamilton, Winifred J. oth Liu, Philip K. oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 74(2000), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:74 year:2000 number:3 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.741098.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 74 2000 3 0 |
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10.1046/j.1471-4159.2000.741098.x doi (DE-627)NLEJ243151225 DE-627 ger DE-627 rakwb Lin, Lie-Huey verfasserin aut Up-Regulation of Base Excision Repair Activity for 8-Hydroxy-2'-Deoxyguanosine in the Mouse Brain After Forebrain Ischemia-Reperfusion Oxford, UK Blackwell Science Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The repair enzyme 8-oxoguanine glycosylase/apyrimidinic/apurinic lyase (OGG) removes 8-hydroxy-2′-deoxyguanosine (oh8dG) in human cells. Our goal was to examine oh8dG-removing activity in the cell nuclei of male C57BL/6 mouse brains treated with either forebrain ischemia-reperfusion (FbIR) or sham operations. We found that the OGG activity in nuclear extracts, under the condition in which other nucleases did not destroy the oligodeoxynucleotide duplex, excised oh8dG with the greatest efficiency on the oligodeoxynucleotide duplex containing oh8dG/dC and with less efficiency on the heteroduplex containing oh8dG/dT, oh8dG/dG, or oh8dG/dA. This specificity was the same as for the recombinant type 1 OGG (OGG1) of humans. We observed that the OGG1 peptide and its activity in the mouse brain were significantly increased after 90 min of ischemia and 20-30 min of reperfusion. The increase in the protein level and in the activity of brain OGG1 correlated positively with the elevation of FbIR-induced DNA lesions in an indicator gene (the c-fos gene) of the brain. The data suggest a possibility that the OGG1 protein may excise oh8dG in the mouse brain and that the activity of OGG1 may have a functional role in reducing oxidative gene damage in the brain after FbIR. 2003 Blackwell Publishing Journal Backfiles 1879-2005 |2003|||||||||| Base excision repair Cao, Shutong verfasserin aut Yu, Ling verfasserin aut Cui, Jiankun oth Hamilton, Winifred J. oth Liu, Philip K. oth In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 74(2000), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:74 year:2000 number:3 pages:0 http://dx.doi.org/10.1046/j.1471-4159.2000.741098.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 74 2000 3 0 |
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10.1046/j.1471-4159.2000.741098.x |
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verfasserin |
| title_sort |
up-regulation of base excision repair activity for 8-hydroxy-2'-deoxyguanosine in the mouse brain after forebrain ischemia-reperfusion |
| title_auth |
Up-Regulation of Base Excision Repair Activity for 8-Hydroxy-2'-Deoxyguanosine in the Mouse Brain After Forebrain Ischemia-Reperfusion |
| abstract |
The repair enzyme 8-oxoguanine glycosylase/apyrimidinic/apurinic lyase (OGG) removes 8-hydroxy-2′-deoxyguanosine (oh8dG) in human cells. Our goal was to examine oh8dG-removing activity in the cell nuclei of male C57BL/6 mouse brains treated with either forebrain ischemia-reperfusion (FbIR) or sham operations. We found that the OGG activity in nuclear extracts, under the condition in which other nucleases did not destroy the oligodeoxynucleotide duplex, excised oh8dG with the greatest efficiency on the oligodeoxynucleotide duplex containing oh8dG/dC and with less efficiency on the heteroduplex containing oh8dG/dT, oh8dG/dG, or oh8dG/dA. This specificity was the same as for the recombinant type 1 OGG (OGG1) of humans. We observed that the OGG1 peptide and its activity in the mouse brain were significantly increased after 90 min of ischemia and 20-30 min of reperfusion. The increase in the protein level and in the activity of brain OGG1 correlated positively with the elevation of FbIR-induced DNA lesions in an indicator gene (the c-fos gene) of the brain. The data suggest a possibility that the OGG1 protein may excise oh8dG in the mouse brain and that the activity of OGG1 may have a functional role in reducing oxidative gene damage in the brain after FbIR. |
| abstractGer |
The repair enzyme 8-oxoguanine glycosylase/apyrimidinic/apurinic lyase (OGG) removes 8-hydroxy-2′-deoxyguanosine (oh8dG) in human cells. Our goal was to examine oh8dG-removing activity in the cell nuclei of male C57BL/6 mouse brains treated with either forebrain ischemia-reperfusion (FbIR) or sham operations. We found that the OGG activity in nuclear extracts, under the condition in which other nucleases did not destroy the oligodeoxynucleotide duplex, excised oh8dG with the greatest efficiency on the oligodeoxynucleotide duplex containing oh8dG/dC and with less efficiency on the heteroduplex containing oh8dG/dT, oh8dG/dG, or oh8dG/dA. This specificity was the same as for the recombinant type 1 OGG (OGG1) of humans. We observed that the OGG1 peptide and its activity in the mouse brain were significantly increased after 90 min of ischemia and 20-30 min of reperfusion. The increase in the protein level and in the activity of brain OGG1 correlated positively with the elevation of FbIR-induced DNA lesions in an indicator gene (the c-fos gene) of the brain. The data suggest a possibility that the OGG1 protein may excise oh8dG in the mouse brain and that the activity of OGG1 may have a functional role in reducing oxidative gene damage in the brain after FbIR. |
| abstract_unstemmed |
The repair enzyme 8-oxoguanine glycosylase/apyrimidinic/apurinic lyase (OGG) removes 8-hydroxy-2′-deoxyguanosine (oh8dG) in human cells. Our goal was to examine oh8dG-removing activity in the cell nuclei of male C57BL/6 mouse brains treated with either forebrain ischemia-reperfusion (FbIR) or sham operations. We found that the OGG activity in nuclear extracts, under the condition in which other nucleases did not destroy the oligodeoxynucleotide duplex, excised oh8dG with the greatest efficiency on the oligodeoxynucleotide duplex containing oh8dG/dC and with less efficiency on the heteroduplex containing oh8dG/dT, oh8dG/dG, or oh8dG/dA. This specificity was the same as for the recombinant type 1 OGG (OGG1) of humans. We observed that the OGG1 peptide and its activity in the mouse brain were significantly increased after 90 min of ischemia and 20-30 min of reperfusion. The increase in the protein level and in the activity of brain OGG1 correlated positively with the elevation of FbIR-induced DNA lesions in an indicator gene (the c-fos gene) of the brain. The data suggest a possibility that the OGG1 protein may excise oh8dG in the mouse brain and that the activity of OGG1 may have a functional role in reducing oxidative gene damage in the brain after FbIR. |
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Up-Regulation of Base Excision Repair Activity for 8-Hydroxy-2'-Deoxyguanosine in the Mouse Brain After Forebrain Ischemia-Reperfusion |
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Cao, Shutong Yu, Ling Cui, Jiankun Hamilton, Winifred J. Liu, Philip K. |
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2024-07-06T04:29:05.332Z |
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