Corticosteroid Modulation of Signal Transduction in the CATH.a Cell Line
Abstract: Noradrenergic neuronal networks originating in the locus coeruleus have been implicated in the stress response. In order to study this system in vitro, we have employed a locus coeruleus-like cell line, CATH.a, and have determined the effect of dexamethasone on receptor-mediated second mes...
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| Autor*in: |
Bundey, Richard A. [verfasserIn] Iredale, Philip A. [verfasserIn] Kendall, David A. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Science Ltd ; 1997 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2002 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Journal of neurochemistry - Oxford : Wiley-Blackwell, 1956, 68(1997), 1, Seite 0 |
| Übergeordnetes Werk: |
volume:68 ; year:1997 ; number:1 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1046/j.1471-4159.1997.68010362.x |
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NLEJ243170998 |
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| 520 | |a Abstract: Noradrenergic neuronal networks originating in the locus coeruleus have been implicated in the stress response. In order to study this system in vitro, we have employed a locus coeruleus-like cell line, CATH.a, and have determined the effect of dexamethasone on receptor-mediated second messenger responses. The CATH.a cell line produced increases in intracellular cyclic AMP conversion in response to corticotrophin-releasing factor (EC50 = 6.93 ± 1.26 nM, maximum conversion = 4.11 ± 0.20%) and vasoactive intestinal polypeptide (EC50 = 240 ± 40 nM, maximum conversion = 8.92 ± 1.24%). Forskolin (10 µM) increased conversion from 0.48 ± 0.05 to 6.39 ± 0.38%. The α2-adrenoceptor agonist 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) inhibited the forskolin response with an IC50 of 6.76 ± 0.11 nM. Carbachol increased total 3H-labelled inositol phosphate accumulation to a maximum of 3.01 ± 0.79 fold basal (EC50 = 7.94 ± 0.14 µM). Bradykinin produced a maximum 1.81 ± 0.05 fold basal stimulation of phosphoinositide hydrolysis (EC50 = 9.12 ± 0.16 nM). Both carbachol and bradykinin increased intracellular Ca2+ concentration probably via a combination of mobilisation of intracellular stores and gating of extracellular Ca2+. Incubation for 24 h with the glucocorticoid receptor agonist, dexamethasone (1 µM), significantly potentiated the receptor-mediated phosphoinositide responses to all the agents tested; however, of the receptor-mediated increases in cyclic AMP conversion, only the vasoactive intestinal polypeptide response was potentiated. These results show that the CATH.a cell line displays some of the properties expected of locus coeruleus neurons and that glucocorticoid receptor stimulation selectively modulates receptor-mediated increases in second messenger formation. | ||
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10.1046/j.1471-4159.1997.68010362.x doi (DE-627)NLEJ243170998 DE-627 ger DE-627 rakwb Bundey, Richard A. verfasserin aut Corticosteroid Modulation of Signal Transduction in the CATH.a Cell Line Oxford, UK Blackwell Science Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Noradrenergic neuronal networks originating in the locus coeruleus have been implicated in the stress response. In order to study this system in vitro, we have employed a locus coeruleus-like cell line, CATH.a, and have determined the effect of dexamethasone on receptor-mediated second messenger responses. The CATH.a cell line produced increases in intracellular cyclic AMP conversion in response to corticotrophin-releasing factor (EC50 = 6.93 ± 1.26 nM, maximum conversion = 4.11 ± 0.20%) and vasoactive intestinal polypeptide (EC50 = 240 ± 40 nM, maximum conversion = 8.92 ± 1.24%). Forskolin (10 µM) increased conversion from 0.48 ± 0.05 to 6.39 ± 0.38%. The α2-adrenoceptor agonist 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) inhibited the forskolin response with an IC50 of 6.76 ± 0.11 nM. Carbachol increased total 3H-labelled inositol phosphate accumulation to a maximum of 3.01 ± 0.79 fold basal (EC50 = 7.94 ± 0.14 µM). Bradykinin produced a maximum 1.81 ± 0.05 fold basal stimulation of phosphoinositide hydrolysis (EC50 = 9.12 ± 0.16 nM). Both carbachol and bradykinin increased intracellular Ca2+ concentration probably via a combination of mobilisation of intracellular stores and gating of extracellular Ca2+. Incubation for 24 h with the glucocorticoid receptor agonist, dexamethasone (1 µM), significantly potentiated the receptor-mediated phosphoinositide responses to all the agents tested; however, of the receptor-mediated increases in cyclic AMP conversion, only the vasoactive intestinal polypeptide response was potentiated. These results show that the CATH.a cell line displays some of the properties expected of locus coeruleus neurons and that glucocorticoid receptor stimulation selectively modulates receptor-mediated increases in second messenger formation. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| CATH.a cell line Iredale, Philip A. verfasserin aut Kendall, David A. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 68(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:68 year:1997 number:1 pages:0 http://dx.doi.org/10.1046/j.1471-4159.1997.68010362.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 68 1997 1 0 |
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10.1046/j.1471-4159.1997.68010362.x doi (DE-627)NLEJ243170998 DE-627 ger DE-627 rakwb Bundey, Richard A. verfasserin aut Corticosteroid Modulation of Signal Transduction in the CATH.a Cell Line Oxford, UK Blackwell Science Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Noradrenergic neuronal networks originating in the locus coeruleus have been implicated in the stress response. In order to study this system in vitro, we have employed a locus coeruleus-like cell line, CATH.a, and have determined the effect of dexamethasone on receptor-mediated second messenger responses. The CATH.a cell line produced increases in intracellular cyclic AMP conversion in response to corticotrophin-releasing factor (EC50 = 6.93 ± 1.26 nM, maximum conversion = 4.11 ± 0.20%) and vasoactive intestinal polypeptide (EC50 = 240 ± 40 nM, maximum conversion = 8.92 ± 1.24%). Forskolin (10 µM) increased conversion from 0.48 ± 0.05 to 6.39 ± 0.38%. The α2-adrenoceptor agonist 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) inhibited the forskolin response with an IC50 of 6.76 ± 0.11 nM. Carbachol increased total 3H-labelled inositol phosphate accumulation to a maximum of 3.01 ± 0.79 fold basal (EC50 = 7.94 ± 0.14 µM). Bradykinin produced a maximum 1.81 ± 0.05 fold basal stimulation of phosphoinositide hydrolysis (EC50 = 9.12 ± 0.16 nM). Both carbachol and bradykinin increased intracellular Ca2+ concentration probably via a combination of mobilisation of intracellular stores and gating of extracellular Ca2+. Incubation for 24 h with the glucocorticoid receptor agonist, dexamethasone (1 µM), significantly potentiated the receptor-mediated phosphoinositide responses to all the agents tested; however, of the receptor-mediated increases in cyclic AMP conversion, only the vasoactive intestinal polypeptide response was potentiated. These results show that the CATH.a cell line displays some of the properties expected of locus coeruleus neurons and that glucocorticoid receptor stimulation selectively modulates receptor-mediated increases in second messenger formation. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| CATH.a cell line Iredale, Philip A. verfasserin aut Kendall, David A. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 68(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:68 year:1997 number:1 pages:0 http://dx.doi.org/10.1046/j.1471-4159.1997.68010362.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 68 1997 1 0 |
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10.1046/j.1471-4159.1997.68010362.x doi (DE-627)NLEJ243170998 DE-627 ger DE-627 rakwb Bundey, Richard A. verfasserin aut Corticosteroid Modulation of Signal Transduction in the CATH.a Cell Line Oxford, UK Blackwell Science Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Noradrenergic neuronal networks originating in the locus coeruleus have been implicated in the stress response. In order to study this system in vitro, we have employed a locus coeruleus-like cell line, CATH.a, and have determined the effect of dexamethasone on receptor-mediated second messenger responses. The CATH.a cell line produced increases in intracellular cyclic AMP conversion in response to corticotrophin-releasing factor (EC50 = 6.93 ± 1.26 nM, maximum conversion = 4.11 ± 0.20%) and vasoactive intestinal polypeptide (EC50 = 240 ± 40 nM, maximum conversion = 8.92 ± 1.24%). Forskolin (10 µM) increased conversion from 0.48 ± 0.05 to 6.39 ± 0.38%. The α2-adrenoceptor agonist 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) inhibited the forskolin response with an IC50 of 6.76 ± 0.11 nM. Carbachol increased total 3H-labelled inositol phosphate accumulation to a maximum of 3.01 ± 0.79 fold basal (EC50 = 7.94 ± 0.14 µM). Bradykinin produced a maximum 1.81 ± 0.05 fold basal stimulation of phosphoinositide hydrolysis (EC50 = 9.12 ± 0.16 nM). Both carbachol and bradykinin increased intracellular Ca2+ concentration probably via a combination of mobilisation of intracellular stores and gating of extracellular Ca2+. Incubation for 24 h with the glucocorticoid receptor agonist, dexamethasone (1 µM), significantly potentiated the receptor-mediated phosphoinositide responses to all the agents tested; however, of the receptor-mediated increases in cyclic AMP conversion, only the vasoactive intestinal polypeptide response was potentiated. These results show that the CATH.a cell line displays some of the properties expected of locus coeruleus neurons and that glucocorticoid receptor stimulation selectively modulates receptor-mediated increases in second messenger formation. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| CATH.a cell line Iredale, Philip A. verfasserin aut Kendall, David A. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 68(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:68 year:1997 number:1 pages:0 http://dx.doi.org/10.1046/j.1471-4159.1997.68010362.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 68 1997 1 0 |
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10.1046/j.1471-4159.1997.68010362.x doi (DE-627)NLEJ243170998 DE-627 ger DE-627 rakwb Bundey, Richard A. verfasserin aut Corticosteroid Modulation of Signal Transduction in the CATH.a Cell Line Oxford, UK Blackwell Science Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Noradrenergic neuronal networks originating in the locus coeruleus have been implicated in the stress response. In order to study this system in vitro, we have employed a locus coeruleus-like cell line, CATH.a, and have determined the effect of dexamethasone on receptor-mediated second messenger responses. The CATH.a cell line produced increases in intracellular cyclic AMP conversion in response to corticotrophin-releasing factor (EC50 = 6.93 ± 1.26 nM, maximum conversion = 4.11 ± 0.20%) and vasoactive intestinal polypeptide (EC50 = 240 ± 40 nM, maximum conversion = 8.92 ± 1.24%). Forskolin (10 µM) increased conversion from 0.48 ± 0.05 to 6.39 ± 0.38%. The α2-adrenoceptor agonist 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) inhibited the forskolin response with an IC50 of 6.76 ± 0.11 nM. Carbachol increased total 3H-labelled inositol phosphate accumulation to a maximum of 3.01 ± 0.79 fold basal (EC50 = 7.94 ± 0.14 µM). Bradykinin produced a maximum 1.81 ± 0.05 fold basal stimulation of phosphoinositide hydrolysis (EC50 = 9.12 ± 0.16 nM). Both carbachol and bradykinin increased intracellular Ca2+ concentration probably via a combination of mobilisation of intracellular stores and gating of extracellular Ca2+. Incubation for 24 h with the glucocorticoid receptor agonist, dexamethasone (1 µM), significantly potentiated the receptor-mediated phosphoinositide responses to all the agents tested; however, of the receptor-mediated increases in cyclic AMP conversion, only the vasoactive intestinal polypeptide response was potentiated. These results show that the CATH.a cell line displays some of the properties expected of locus coeruleus neurons and that glucocorticoid receptor stimulation selectively modulates receptor-mediated increases in second messenger formation. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| CATH.a cell line Iredale, Philip A. verfasserin aut Kendall, David A. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 68(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:68 year:1997 number:1 pages:0 http://dx.doi.org/10.1046/j.1471-4159.1997.68010362.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 68 1997 1 0 |
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10.1046/j.1471-4159.1997.68010362.x doi (DE-627)NLEJ243170998 DE-627 ger DE-627 rakwb Bundey, Richard A. verfasserin aut Corticosteroid Modulation of Signal Transduction in the CATH.a Cell Line Oxford, UK Blackwell Science Ltd 1997 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Abstract: Noradrenergic neuronal networks originating in the locus coeruleus have been implicated in the stress response. In order to study this system in vitro, we have employed a locus coeruleus-like cell line, CATH.a, and have determined the effect of dexamethasone on receptor-mediated second messenger responses. The CATH.a cell line produced increases in intracellular cyclic AMP conversion in response to corticotrophin-releasing factor (EC50 = 6.93 ± 1.26 nM, maximum conversion = 4.11 ± 0.20%) and vasoactive intestinal polypeptide (EC50 = 240 ± 40 nM, maximum conversion = 8.92 ± 1.24%). Forskolin (10 µM) increased conversion from 0.48 ± 0.05 to 6.39 ± 0.38%. The α2-adrenoceptor agonist 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) inhibited the forskolin response with an IC50 of 6.76 ± 0.11 nM. Carbachol increased total 3H-labelled inositol phosphate accumulation to a maximum of 3.01 ± 0.79 fold basal (EC50 = 7.94 ± 0.14 µM). Bradykinin produced a maximum 1.81 ± 0.05 fold basal stimulation of phosphoinositide hydrolysis (EC50 = 9.12 ± 0.16 nM). Both carbachol and bradykinin increased intracellular Ca2+ concentration probably via a combination of mobilisation of intracellular stores and gating of extracellular Ca2+. Incubation for 24 h with the glucocorticoid receptor agonist, dexamethasone (1 µM), significantly potentiated the receptor-mediated phosphoinositide responses to all the agents tested; however, of the receptor-mediated increases in cyclic AMP conversion, only the vasoactive intestinal polypeptide response was potentiated. These results show that the CATH.a cell line displays some of the properties expected of locus coeruleus neurons and that glucocorticoid receptor stimulation selectively modulates receptor-mediated increases in second messenger formation. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| CATH.a cell line Iredale, Philip A. verfasserin aut Kendall, David A. verfasserin aut In Journal of neurochemistry Oxford : Wiley-Blackwell, 1956 68(1997), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927584 (DE-600)2020528-4 1471-4159 nnns volume:68 year:1997 number:1 pages:0 http://dx.doi.org/10.1046/j.1471-4159.1997.68010362.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 68 1997 1 0 |
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Corticosteroid Modulation of Signal Transduction in the CATH.a Cell Line |
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Bundey, Richard A. |
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Journal of neurochemistry |
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1997 |
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Bundey, Richard A. Iredale, Philip A. Kendall, David A. |
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68 |
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Elektronische Aufsätze |
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Bundey, Richard A. |
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10.1046/j.1471-4159.1997.68010362.x |
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verfasserin |
| title_sort |
corticosteroid modulation of signal transduction in the cath.a cell line |
| title_auth |
Corticosteroid Modulation of Signal Transduction in the CATH.a Cell Line |
| abstract |
Abstract: Noradrenergic neuronal networks originating in the locus coeruleus have been implicated in the stress response. In order to study this system in vitro, we have employed a locus coeruleus-like cell line, CATH.a, and have determined the effect of dexamethasone on receptor-mediated second messenger responses. The CATH.a cell line produced increases in intracellular cyclic AMP conversion in response to corticotrophin-releasing factor (EC50 = 6.93 ± 1.26 nM, maximum conversion = 4.11 ± 0.20%) and vasoactive intestinal polypeptide (EC50 = 240 ± 40 nM, maximum conversion = 8.92 ± 1.24%). Forskolin (10 µM) increased conversion from 0.48 ± 0.05 to 6.39 ± 0.38%. The α2-adrenoceptor agonist 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) inhibited the forskolin response with an IC50 of 6.76 ± 0.11 nM. Carbachol increased total 3H-labelled inositol phosphate accumulation to a maximum of 3.01 ± 0.79 fold basal (EC50 = 7.94 ± 0.14 µM). Bradykinin produced a maximum 1.81 ± 0.05 fold basal stimulation of phosphoinositide hydrolysis (EC50 = 9.12 ± 0.16 nM). Both carbachol and bradykinin increased intracellular Ca2+ concentration probably via a combination of mobilisation of intracellular stores and gating of extracellular Ca2+. Incubation for 24 h with the glucocorticoid receptor agonist, dexamethasone (1 µM), significantly potentiated the receptor-mediated phosphoinositide responses to all the agents tested; however, of the receptor-mediated increases in cyclic AMP conversion, only the vasoactive intestinal polypeptide response was potentiated. These results show that the CATH.a cell line displays some of the properties expected of locus coeruleus neurons and that glucocorticoid receptor stimulation selectively modulates receptor-mediated increases in second messenger formation. |
| abstractGer |
Abstract: Noradrenergic neuronal networks originating in the locus coeruleus have been implicated in the stress response. In order to study this system in vitro, we have employed a locus coeruleus-like cell line, CATH.a, and have determined the effect of dexamethasone on receptor-mediated second messenger responses. The CATH.a cell line produced increases in intracellular cyclic AMP conversion in response to corticotrophin-releasing factor (EC50 = 6.93 ± 1.26 nM, maximum conversion = 4.11 ± 0.20%) and vasoactive intestinal polypeptide (EC50 = 240 ± 40 nM, maximum conversion = 8.92 ± 1.24%). Forskolin (10 µM) increased conversion from 0.48 ± 0.05 to 6.39 ± 0.38%. The α2-adrenoceptor agonist 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) inhibited the forskolin response with an IC50 of 6.76 ± 0.11 nM. Carbachol increased total 3H-labelled inositol phosphate accumulation to a maximum of 3.01 ± 0.79 fold basal (EC50 = 7.94 ± 0.14 µM). Bradykinin produced a maximum 1.81 ± 0.05 fold basal stimulation of phosphoinositide hydrolysis (EC50 = 9.12 ± 0.16 nM). Both carbachol and bradykinin increased intracellular Ca2+ concentration probably via a combination of mobilisation of intracellular stores and gating of extracellular Ca2+. Incubation for 24 h with the glucocorticoid receptor agonist, dexamethasone (1 µM), significantly potentiated the receptor-mediated phosphoinositide responses to all the agents tested; however, of the receptor-mediated increases in cyclic AMP conversion, only the vasoactive intestinal polypeptide response was potentiated. These results show that the CATH.a cell line displays some of the properties expected of locus coeruleus neurons and that glucocorticoid receptor stimulation selectively modulates receptor-mediated increases in second messenger formation. |
| abstract_unstemmed |
Abstract: Noradrenergic neuronal networks originating in the locus coeruleus have been implicated in the stress response. In order to study this system in vitro, we have employed a locus coeruleus-like cell line, CATH.a, and have determined the effect of dexamethasone on receptor-mediated second messenger responses. The CATH.a cell line produced increases in intracellular cyclic AMP conversion in response to corticotrophin-releasing factor (EC50 = 6.93 ± 1.26 nM, maximum conversion = 4.11 ± 0.20%) and vasoactive intestinal polypeptide (EC50 = 240 ± 40 nM, maximum conversion = 8.92 ± 1.24%). Forskolin (10 µM) increased conversion from 0.48 ± 0.05 to 6.39 ± 0.38%. The α2-adrenoceptor agonist 5-bromo-N-(4,5-dihydro-1H-imidazol-2-yl)-6-quinoxalinamine (UK14304) inhibited the forskolin response with an IC50 of 6.76 ± 0.11 nM. Carbachol increased total 3H-labelled inositol phosphate accumulation to a maximum of 3.01 ± 0.79 fold basal (EC50 = 7.94 ± 0.14 µM). Bradykinin produced a maximum 1.81 ± 0.05 fold basal stimulation of phosphoinositide hydrolysis (EC50 = 9.12 ± 0.16 nM). Both carbachol and bradykinin increased intracellular Ca2+ concentration probably via a combination of mobilisation of intracellular stores and gating of extracellular Ca2+. Incubation for 24 h with the glucocorticoid receptor agonist, dexamethasone (1 µM), significantly potentiated the receptor-mediated phosphoinositide responses to all the agents tested; however, of the receptor-mediated increases in cyclic AMP conversion, only the vasoactive intestinal polypeptide response was potentiated. These results show that the CATH.a cell line displays some of the properties expected of locus coeruleus neurons and that glucocorticoid receptor stimulation selectively modulates receptor-mediated increases in second messenger formation. |
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Corticosteroid Modulation of Signal Transduction in the CATH.a Cell Line |
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