A PCR-Based Survey on Phytomonas (Euglenozoa+Trypanosomatidae) in Phytophagous Hemipterans of the Amazon Region
. We have surveyed 244 hemipterans from Western Brazilian Amazônia for the presence of trypanosomatids and identification of members of the genus Phytomonas. Examination by phase microscopy of squashes of insect salivary glands (SG) and digestive tubes (DT) revealed that 44% (108/244) of insects fro...
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| Autor*in: |
GODOI, MARA M. I. [verfasserIn] SERRANO, MYRNA G. [verfasserIn] TEIXEIRA, MARTA M. G. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Publishing Ltd ; 2002 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2005 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: The journal of eukaryotic microbiology - Oxford [u.a.] : Wiley-Blackwell, 1954, 49(2002), 4, Seite 0 |
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volume:49 ; year:2002 ; number:4 ; pages:0 |
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| DOI / URN: |
10.1111/j.1550-7408.2002.tb00370.x |
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| 520 | |a . We have surveyed 244 hemipterans from Western Brazilian Amazônia for the presence of trypanosomatids and identification of members of the genus Phytomonas. Examination by phase microscopy of squashes of insect salivary glands (SG) and digestive tubes (DT) revealed that 44% (108/244) of insects from seven families harbored trypanosomatids. Infections were 5 times more frequent in Coreidae than in all other families together. Smears of SG and DT of the dissected insects were fixed on glass slides with methanol and stained with Giemsa for morphological analysis. DNA was recovered from these preparations and submitted to a PCR assay that permitted amplification of all trypanosomatid genera using primers of conserved sequences flanking a segment of the spliced leader (SL) gene. Upon PCR amplification of the recovered DNA, amplicons were hybridized with an oligonucletide probe (SL3′) complementary to a SL intron sequence specific for flagellates of the genus Phytomonas. Among the trypanosomatid-positive insects, 38.8% harbored Phytomonas spp., corresponding to an overall Phytomonas prevalence of 17.1% among phytophagous bugs, their putative vectors. Since many Phytomonas are pathogenic in plants, this high prevalence in their vectors emphasizes the permanent risk of exposure to disease by native and cultured plants of the Amazon region. | ||
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10.1111/j.1550-7408.2002.tb00370.x doi (DE-627)NLEJ24327338X DE-627 ger DE-627 rakwb GODOI, MARA M. I. verfasserin aut A PCR-Based Survey on Phytomonas (Euglenozoa+Trypanosomatidae) in Phytophagous Hemipterans of the Amazon Region Oxford, UK Blackwell Publishing Ltd 2002 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier . We have surveyed 244 hemipterans from Western Brazilian Amazônia for the presence of trypanosomatids and identification of members of the genus Phytomonas. Examination by phase microscopy of squashes of insect salivary glands (SG) and digestive tubes (DT) revealed that 44% (108/244) of insects from seven families harbored trypanosomatids. Infections were 5 times more frequent in Coreidae than in all other families together. Smears of SG and DT of the dissected insects were fixed on glass slides with methanol and stained with Giemsa for morphological analysis. DNA was recovered from these preparations and submitted to a PCR assay that permitted amplification of all trypanosomatid genera using primers of conserved sequences flanking a segment of the spliced leader (SL) gene. Upon PCR amplification of the recovered DNA, amplicons were hybridized with an oligonucletide probe (SL3′) complementary to a SL intron sequence specific for flagellates of the genus Phytomonas. Among the trypanosomatid-positive insects, 38.8% harbored Phytomonas spp., corresponding to an overall Phytomonas prevalence of 17.1% among phytophagous bugs, their putative vectors. Since many Phytomonas are pathogenic in plants, this high prevalence in their vectors emphasizes the permanent risk of exposure to disease by native and cultured plants of the Amazon region. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| Diagnosis SERRANO, MYRNA G. verfasserin aut TEIXEIRA, MARTA M. G. verfasserin aut CAMARGO, ERNEY P. oth In The journal of eukaryotic microbiology Oxford [u.a.] : Wiley-Blackwell, 1954 49(2002), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927622 (DE-600)2126326-7 1550-7408 nnns volume:49 year:2002 number:4 pages:0 http://dx.doi.org/10.1111/j.1550-7408.2002.tb00370.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 49 2002 4 0 |
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10.1111/j.1550-7408.2002.tb00370.x doi (DE-627)NLEJ24327338X DE-627 ger DE-627 rakwb GODOI, MARA M. I. verfasserin aut A PCR-Based Survey on Phytomonas (Euglenozoa+Trypanosomatidae) in Phytophagous Hemipterans of the Amazon Region Oxford, UK Blackwell Publishing Ltd 2002 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier . We have surveyed 244 hemipterans from Western Brazilian Amazônia for the presence of trypanosomatids and identification of members of the genus Phytomonas. Examination by phase microscopy of squashes of insect salivary glands (SG) and digestive tubes (DT) revealed that 44% (108/244) of insects from seven families harbored trypanosomatids. Infections were 5 times more frequent in Coreidae than in all other families together. Smears of SG and DT of the dissected insects were fixed on glass slides with methanol and stained with Giemsa for morphological analysis. DNA was recovered from these preparations and submitted to a PCR assay that permitted amplification of all trypanosomatid genera using primers of conserved sequences flanking a segment of the spliced leader (SL) gene. Upon PCR amplification of the recovered DNA, amplicons were hybridized with an oligonucletide probe (SL3′) complementary to a SL intron sequence specific for flagellates of the genus Phytomonas. Among the trypanosomatid-positive insects, 38.8% harbored Phytomonas spp., corresponding to an overall Phytomonas prevalence of 17.1% among phytophagous bugs, their putative vectors. Since many Phytomonas are pathogenic in plants, this high prevalence in their vectors emphasizes the permanent risk of exposure to disease by native and cultured plants of the Amazon region. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| Diagnosis SERRANO, MYRNA G. verfasserin aut TEIXEIRA, MARTA M. G. verfasserin aut CAMARGO, ERNEY P. oth In The journal of eukaryotic microbiology Oxford [u.a.] : Wiley-Blackwell, 1954 49(2002), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927622 (DE-600)2126326-7 1550-7408 nnns volume:49 year:2002 number:4 pages:0 http://dx.doi.org/10.1111/j.1550-7408.2002.tb00370.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 49 2002 4 0 |
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10.1111/j.1550-7408.2002.tb00370.x doi (DE-627)NLEJ24327338X DE-627 ger DE-627 rakwb GODOI, MARA M. I. verfasserin aut A PCR-Based Survey on Phytomonas (Euglenozoa+Trypanosomatidae) in Phytophagous Hemipterans of the Amazon Region Oxford, UK Blackwell Publishing Ltd 2002 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier . We have surveyed 244 hemipterans from Western Brazilian Amazônia for the presence of trypanosomatids and identification of members of the genus Phytomonas. Examination by phase microscopy of squashes of insect salivary glands (SG) and digestive tubes (DT) revealed that 44% (108/244) of insects from seven families harbored trypanosomatids. Infections were 5 times more frequent in Coreidae than in all other families together. Smears of SG and DT of the dissected insects were fixed on glass slides with methanol and stained with Giemsa for morphological analysis. DNA was recovered from these preparations and submitted to a PCR assay that permitted amplification of all trypanosomatid genera using primers of conserved sequences flanking a segment of the spliced leader (SL) gene. Upon PCR amplification of the recovered DNA, amplicons were hybridized with an oligonucletide probe (SL3′) complementary to a SL intron sequence specific for flagellates of the genus Phytomonas. Among the trypanosomatid-positive insects, 38.8% harbored Phytomonas spp., corresponding to an overall Phytomonas prevalence of 17.1% among phytophagous bugs, their putative vectors. Since many Phytomonas are pathogenic in plants, this high prevalence in their vectors emphasizes the permanent risk of exposure to disease by native and cultured plants of the Amazon region. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| Diagnosis SERRANO, MYRNA G. verfasserin aut TEIXEIRA, MARTA M. G. verfasserin aut CAMARGO, ERNEY P. oth In The journal of eukaryotic microbiology Oxford [u.a.] : Wiley-Blackwell, 1954 49(2002), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927622 (DE-600)2126326-7 1550-7408 nnns volume:49 year:2002 number:4 pages:0 http://dx.doi.org/10.1111/j.1550-7408.2002.tb00370.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 49 2002 4 0 |
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10.1111/j.1550-7408.2002.tb00370.x doi (DE-627)NLEJ24327338X DE-627 ger DE-627 rakwb GODOI, MARA M. I. verfasserin aut A PCR-Based Survey on Phytomonas (Euglenozoa+Trypanosomatidae) in Phytophagous Hemipterans of the Amazon Region Oxford, UK Blackwell Publishing Ltd 2002 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier . We have surveyed 244 hemipterans from Western Brazilian Amazônia for the presence of trypanosomatids and identification of members of the genus Phytomonas. Examination by phase microscopy of squashes of insect salivary glands (SG) and digestive tubes (DT) revealed that 44% (108/244) of insects from seven families harbored trypanosomatids. Infections were 5 times more frequent in Coreidae than in all other families together. Smears of SG and DT of the dissected insects were fixed on glass slides with methanol and stained with Giemsa for morphological analysis. DNA was recovered from these preparations and submitted to a PCR assay that permitted amplification of all trypanosomatid genera using primers of conserved sequences flanking a segment of the spliced leader (SL) gene. Upon PCR amplification of the recovered DNA, amplicons were hybridized with an oligonucletide probe (SL3′) complementary to a SL intron sequence specific for flagellates of the genus Phytomonas. Among the trypanosomatid-positive insects, 38.8% harbored Phytomonas spp., corresponding to an overall Phytomonas prevalence of 17.1% among phytophagous bugs, their putative vectors. Since many Phytomonas are pathogenic in plants, this high prevalence in their vectors emphasizes the permanent risk of exposure to disease by native and cultured plants of the Amazon region. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| Diagnosis SERRANO, MYRNA G. verfasserin aut TEIXEIRA, MARTA M. G. verfasserin aut CAMARGO, ERNEY P. oth In The journal of eukaryotic microbiology Oxford [u.a.] : Wiley-Blackwell, 1954 49(2002), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927622 (DE-600)2126326-7 1550-7408 nnns volume:49 year:2002 number:4 pages:0 http://dx.doi.org/10.1111/j.1550-7408.2002.tb00370.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 49 2002 4 0 |
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10.1111/j.1550-7408.2002.tb00370.x doi (DE-627)NLEJ24327338X DE-627 ger DE-627 rakwb GODOI, MARA M. I. verfasserin aut A PCR-Based Survey on Phytomonas (Euglenozoa+Trypanosomatidae) in Phytophagous Hemipterans of the Amazon Region Oxford, UK Blackwell Publishing Ltd 2002 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier . We have surveyed 244 hemipterans from Western Brazilian Amazônia for the presence of trypanosomatids and identification of members of the genus Phytomonas. Examination by phase microscopy of squashes of insect salivary glands (SG) and digestive tubes (DT) revealed that 44% (108/244) of insects from seven families harbored trypanosomatids. Infections were 5 times more frequent in Coreidae than in all other families together. Smears of SG and DT of the dissected insects were fixed on glass slides with methanol and stained with Giemsa for morphological analysis. DNA was recovered from these preparations and submitted to a PCR assay that permitted amplification of all trypanosomatid genera using primers of conserved sequences flanking a segment of the spliced leader (SL) gene. Upon PCR amplification of the recovered DNA, amplicons were hybridized with an oligonucletide probe (SL3′) complementary to a SL intron sequence specific for flagellates of the genus Phytomonas. Among the trypanosomatid-positive insects, 38.8% harbored Phytomonas spp., corresponding to an overall Phytomonas prevalence of 17.1% among phytophagous bugs, their putative vectors. Since many Phytomonas are pathogenic in plants, this high prevalence in their vectors emphasizes the permanent risk of exposure to disease by native and cultured plants of the Amazon region. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| Diagnosis SERRANO, MYRNA G. verfasserin aut TEIXEIRA, MARTA M. G. verfasserin aut CAMARGO, ERNEY P. oth In The journal of eukaryotic microbiology Oxford [u.a.] : Wiley-Blackwell, 1954 49(2002), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927622 (DE-600)2126326-7 1550-7408 nnns volume:49 year:2002 number:4 pages:0 http://dx.doi.org/10.1111/j.1550-7408.2002.tb00370.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 49 2002 4 0 |
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A PCR-Based Survey on Phytomonas (Euglenozoa+Trypanosomatidae) in Phytophagous Hemipterans of the Amazon Region |
| abstract |
. We have surveyed 244 hemipterans from Western Brazilian Amazônia for the presence of trypanosomatids and identification of members of the genus Phytomonas. Examination by phase microscopy of squashes of insect salivary glands (SG) and digestive tubes (DT) revealed that 44% (108/244) of insects from seven families harbored trypanosomatids. Infections were 5 times more frequent in Coreidae than in all other families together. Smears of SG and DT of the dissected insects were fixed on glass slides with methanol and stained with Giemsa for morphological analysis. DNA was recovered from these preparations and submitted to a PCR assay that permitted amplification of all trypanosomatid genera using primers of conserved sequences flanking a segment of the spliced leader (SL) gene. Upon PCR amplification of the recovered DNA, amplicons were hybridized with an oligonucletide probe (SL3′) complementary to a SL intron sequence specific for flagellates of the genus Phytomonas. Among the trypanosomatid-positive insects, 38.8% harbored Phytomonas spp., corresponding to an overall Phytomonas prevalence of 17.1% among phytophagous bugs, their putative vectors. Since many Phytomonas are pathogenic in plants, this high prevalence in their vectors emphasizes the permanent risk of exposure to disease by native and cultured plants of the Amazon region. |
| abstractGer |
. We have surveyed 244 hemipterans from Western Brazilian Amazônia for the presence of trypanosomatids and identification of members of the genus Phytomonas. Examination by phase microscopy of squashes of insect salivary glands (SG) and digestive tubes (DT) revealed that 44% (108/244) of insects from seven families harbored trypanosomatids. Infections were 5 times more frequent in Coreidae than in all other families together. Smears of SG and DT of the dissected insects were fixed on glass slides with methanol and stained with Giemsa for morphological analysis. DNA was recovered from these preparations and submitted to a PCR assay that permitted amplification of all trypanosomatid genera using primers of conserved sequences flanking a segment of the spliced leader (SL) gene. Upon PCR amplification of the recovered DNA, amplicons were hybridized with an oligonucletide probe (SL3′) complementary to a SL intron sequence specific for flagellates of the genus Phytomonas. Among the trypanosomatid-positive insects, 38.8% harbored Phytomonas spp., corresponding to an overall Phytomonas prevalence of 17.1% among phytophagous bugs, their putative vectors. Since many Phytomonas are pathogenic in plants, this high prevalence in their vectors emphasizes the permanent risk of exposure to disease by native and cultured plants of the Amazon region. |
| abstract_unstemmed |
. We have surveyed 244 hemipterans from Western Brazilian Amazônia for the presence of trypanosomatids and identification of members of the genus Phytomonas. Examination by phase microscopy of squashes of insect salivary glands (SG) and digestive tubes (DT) revealed that 44% (108/244) of insects from seven families harbored trypanosomatids. Infections were 5 times more frequent in Coreidae than in all other families together. Smears of SG and DT of the dissected insects were fixed on glass slides with methanol and stained with Giemsa for morphological analysis. DNA was recovered from these preparations and submitted to a PCR assay that permitted amplification of all trypanosomatid genera using primers of conserved sequences flanking a segment of the spliced leader (SL) gene. Upon PCR amplification of the recovered DNA, amplicons were hybridized with an oligonucletide probe (SL3′) complementary to a SL intron sequence specific for flagellates of the genus Phytomonas. Among the trypanosomatid-positive insects, 38.8% harbored Phytomonas spp., corresponding to an overall Phytomonas prevalence of 17.1% among phytophagous bugs, their putative vectors. Since many Phytomonas are pathogenic in plants, this high prevalence in their vectors emphasizes the permanent risk of exposure to disease by native and cultured plants of the Amazon region. |
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| title_short |
A PCR-Based Survey on Phytomonas (Euglenozoa+Trypanosomatidae) in Phytophagous Hemipterans of the Amazon Region |
| url |
http://dx.doi.org/10.1111/j.1550-7408.2002.tb00370.x |
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SERRANO, MYRNA G. TEIXEIRA, MARTA M. G. CAMARGO, ERNEY P. |
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| doi_str |
10.1111/j.1550-7408.2002.tb00370.x |
| up_date |
2024-07-06T04:54:51.125Z |
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