Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples
Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real...
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Gespeichert in:
| Autor*in: |
Jervøe-Storm, P.-M. [verfasserIn] Koltzscher, M. [verfasserIn] Falk, W. [verfasserIn] |
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E-Artikel |
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| Erschienen: |
Oxford, UK: Munksgaard International Publishers ; 2005 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2005 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Journal of clinical periodontology - Oxford [u.a.] : Wiley-Blackwell, 1974, 32(2005), 7, Seite 0 |
| Übergeordnetes Werk: |
volume:32 ; year:2005 ; number:7 ; pages:0 |
| Links: |
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| DOI / URN: |
10.1111/j.1600-051X.2005.00740.x |
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| Katalog-ID: |
NLEJ243319800 |
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| 100 | 1 | |a Jervøe-Storm, P.-M. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples |
| 264 | 1 | |a Oxford, UK |b Munksgaard International Publishers |c 2005 | |
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| 520 | |a Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real-time PCR with conventional anaerobic cultivation.Method: A total of 78 subgingival plaque samples were harvested from pockets <inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE740:ges" location="ges.gif"/>5 mm in 22 patients with advanced chronic periodontitis and immediately transferred into transport medium. Aliquots were evaluated with species-specific probes by real-time PCR (meridol® Perio Diagnostics, GABA) and anaerobic bacteria culture on selective media for the detection of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis. The analysis was performed by two separate, blinded examiners.Results: When real-time PCR was compared with the culture method (golden standard) for the detection of putative periodontopathogenic bacteria, the sensitivity and specificity for A. actinomycetemcomitans were 67% and 100%, respectively (κ: 0.79); for F. nucleatum 73% and 53%, respectively (κ: 0.21); for P. gingivalis 94% and 84%, respectively (κ: 0.77); for P. intermedia 33% and 94%, respectively (κ: 0.26) and for T. forsythensis 92% and 56%, respectively (κ: 0.51). Spearman's correlation coefficients for the quantitative results of both methods were 0.82 for A. actinomycetemcomitans, 0.33 for F. nucleatum, 0.83 for P. gingivalis, 0.38 for P. intermedia and 0.67 for T. forsythensis.Conclusion: Overall, the agreement between both test methods was excellent for A. actinomycetemcomitans and P. gingivalis, fair for T. forsythensis and poor for F. nucleatum and P. intermedia. The discrepancies in the results may be explained by the inability of cultivation methods to distinguish between close related taxa, and the problems of keeping periopathogenic bacteria viable, which is required for standard cultivation. | ||
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| 700 | 1 | |a Koltzscher, M. |e verfasserin |4 aut | |
| 700 | 1 | |a Falk, W. |e verfasserin |4 aut | |
| 700 | 1 | |a Dörfler, A. |4 oth | |
| 700 | 1 | |a Jepsen, S. |4 oth | |
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10.1111/j.1600-051X.2005.00740.x doi (DE-627)NLEJ243319800 DE-627 ger DE-627 rakwb Jervøe-Storm, P.-M. verfasserin aut Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real-time PCR with conventional anaerobic cultivation.Method: A total of 78 subgingival plaque samples were harvested from pockets <inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE740:ges" location="ges.gif"/>5 mm in 22 patients with advanced chronic periodontitis and immediately transferred into transport medium. Aliquots were evaluated with species-specific probes by real-time PCR (meridol® Perio Diagnostics, GABA) and anaerobic bacteria culture on selective media for the detection of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis. The analysis was performed by two separate, blinded examiners.Results: When real-time PCR was compared with the culture method (golden standard) for the detection of putative periodontopathogenic bacteria, the sensitivity and specificity for A. actinomycetemcomitans were 67% and 100%, respectively (κ: 0.79); for F. nucleatum 73% and 53%, respectively (κ: 0.21); for P. gingivalis 94% and 84%, respectively (κ: 0.77); for P. intermedia 33% and 94%, respectively (κ: 0.26) and for T. forsythensis 92% and 56%, respectively (κ: 0.51). Spearman's correlation coefficients for the quantitative results of both methods were 0.82 for A. actinomycetemcomitans, 0.33 for F. nucleatum, 0.83 for P. gingivalis, 0.38 for P. intermedia and 0.67 for T. forsythensis.Conclusion: Overall, the agreement between both test methods was excellent for A. actinomycetemcomitans and P. gingivalis, fair for T. forsythensis and poor for F. nucleatum and P. intermedia. The discrepancies in the results may be explained by the inability of cultivation methods to distinguish between close related taxa, and the problems of keeping periopathogenic bacteria viable, which is required for standard cultivation. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| bacterial culture Koltzscher, M. verfasserin aut Falk, W. verfasserin aut Dörfler, A. oth Jepsen, S. oth In Journal of clinical periodontology Oxford [u.a.] : Wiley-Blackwell, 1974 32(2005), 7, Seite 0 Online-Ressource (DE-627)NLEJ243927142 (DE-600)2026349-1 1600-051X nnns volume:32 year:2005 number:7 pages:0 http://dx.doi.org/10.1111/j.1600-051X.2005.00740.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 2005 7 0 |
| spelling |
10.1111/j.1600-051X.2005.00740.x doi (DE-627)NLEJ243319800 DE-627 ger DE-627 rakwb Jervøe-Storm, P.-M. verfasserin aut Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real-time PCR with conventional anaerobic cultivation.Method: A total of 78 subgingival plaque samples were harvested from pockets <inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE740:ges" location="ges.gif"/>5 mm in 22 patients with advanced chronic periodontitis and immediately transferred into transport medium. Aliquots were evaluated with species-specific probes by real-time PCR (meridol® Perio Diagnostics, GABA) and anaerobic bacteria culture on selective media for the detection of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis. The analysis was performed by two separate, blinded examiners.Results: When real-time PCR was compared with the culture method (golden standard) for the detection of putative periodontopathogenic bacteria, the sensitivity and specificity for A. actinomycetemcomitans were 67% and 100%, respectively (κ: 0.79); for F. nucleatum 73% and 53%, respectively (κ: 0.21); for P. gingivalis 94% and 84%, respectively (κ: 0.77); for P. intermedia 33% and 94%, respectively (κ: 0.26) and for T. forsythensis 92% and 56%, respectively (κ: 0.51). Spearman's correlation coefficients for the quantitative results of both methods were 0.82 for A. actinomycetemcomitans, 0.33 for F. nucleatum, 0.83 for P. gingivalis, 0.38 for P. intermedia and 0.67 for T. forsythensis.Conclusion: Overall, the agreement between both test methods was excellent for A. actinomycetemcomitans and P. gingivalis, fair for T. forsythensis and poor for F. nucleatum and P. intermedia. The discrepancies in the results may be explained by the inability of cultivation methods to distinguish between close related taxa, and the problems of keeping periopathogenic bacteria viable, which is required for standard cultivation. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| bacterial culture Koltzscher, M. verfasserin aut Falk, W. verfasserin aut Dörfler, A. oth Jepsen, S. oth In Journal of clinical periodontology Oxford [u.a.] : Wiley-Blackwell, 1974 32(2005), 7, Seite 0 Online-Ressource (DE-627)NLEJ243927142 (DE-600)2026349-1 1600-051X nnns volume:32 year:2005 number:7 pages:0 http://dx.doi.org/10.1111/j.1600-051X.2005.00740.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 2005 7 0 |
| allfields_unstemmed |
10.1111/j.1600-051X.2005.00740.x doi (DE-627)NLEJ243319800 DE-627 ger DE-627 rakwb Jervøe-Storm, P.-M. verfasserin aut Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real-time PCR with conventional anaerobic cultivation.Method: A total of 78 subgingival plaque samples were harvested from pockets <inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE740:ges" location="ges.gif"/>5 mm in 22 patients with advanced chronic periodontitis and immediately transferred into transport medium. Aliquots were evaluated with species-specific probes by real-time PCR (meridol® Perio Diagnostics, GABA) and anaerobic bacteria culture on selective media for the detection of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis. The analysis was performed by two separate, blinded examiners.Results: When real-time PCR was compared with the culture method (golden standard) for the detection of putative periodontopathogenic bacteria, the sensitivity and specificity for A. actinomycetemcomitans were 67% and 100%, respectively (κ: 0.79); for F. nucleatum 73% and 53%, respectively (κ: 0.21); for P. gingivalis 94% and 84%, respectively (κ: 0.77); for P. intermedia 33% and 94%, respectively (κ: 0.26) and for T. forsythensis 92% and 56%, respectively (κ: 0.51). Spearman's correlation coefficients for the quantitative results of both methods were 0.82 for A. actinomycetemcomitans, 0.33 for F. nucleatum, 0.83 for P. gingivalis, 0.38 for P. intermedia and 0.67 for T. forsythensis.Conclusion: Overall, the agreement between both test methods was excellent for A. actinomycetemcomitans and P. gingivalis, fair for T. forsythensis and poor for F. nucleatum and P. intermedia. The discrepancies in the results may be explained by the inability of cultivation methods to distinguish between close related taxa, and the problems of keeping periopathogenic bacteria viable, which is required for standard cultivation. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| bacterial culture Koltzscher, M. verfasserin aut Falk, W. verfasserin aut Dörfler, A. oth Jepsen, S. oth In Journal of clinical periodontology Oxford [u.a.] : Wiley-Blackwell, 1974 32(2005), 7, Seite 0 Online-Ressource (DE-627)NLEJ243927142 (DE-600)2026349-1 1600-051X nnns volume:32 year:2005 number:7 pages:0 http://dx.doi.org/10.1111/j.1600-051X.2005.00740.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 2005 7 0 |
| allfieldsGer |
10.1111/j.1600-051X.2005.00740.x doi (DE-627)NLEJ243319800 DE-627 ger DE-627 rakwb Jervøe-Storm, P.-M. verfasserin aut Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real-time PCR with conventional anaerobic cultivation.Method: A total of 78 subgingival plaque samples were harvested from pockets <inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE740:ges" location="ges.gif"/>5 mm in 22 patients with advanced chronic periodontitis and immediately transferred into transport medium. Aliquots were evaluated with species-specific probes by real-time PCR (meridol® Perio Diagnostics, GABA) and anaerobic bacteria culture on selective media for the detection of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis. The analysis was performed by two separate, blinded examiners.Results: When real-time PCR was compared with the culture method (golden standard) for the detection of putative periodontopathogenic bacteria, the sensitivity and specificity for A. actinomycetemcomitans were 67% and 100%, respectively (κ: 0.79); for F. nucleatum 73% and 53%, respectively (κ: 0.21); for P. gingivalis 94% and 84%, respectively (κ: 0.77); for P. intermedia 33% and 94%, respectively (κ: 0.26) and for T. forsythensis 92% and 56%, respectively (κ: 0.51). Spearman's correlation coefficients for the quantitative results of both methods were 0.82 for A. actinomycetemcomitans, 0.33 for F. nucleatum, 0.83 for P. gingivalis, 0.38 for P. intermedia and 0.67 for T. forsythensis.Conclusion: Overall, the agreement between both test methods was excellent for A. actinomycetemcomitans and P. gingivalis, fair for T. forsythensis and poor for F. nucleatum and P. intermedia. The discrepancies in the results may be explained by the inability of cultivation methods to distinguish between close related taxa, and the problems of keeping periopathogenic bacteria viable, which is required for standard cultivation. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| bacterial culture Koltzscher, M. verfasserin aut Falk, W. verfasserin aut Dörfler, A. oth Jepsen, S. oth In Journal of clinical periodontology Oxford [u.a.] : Wiley-Blackwell, 1974 32(2005), 7, Seite 0 Online-Ressource (DE-627)NLEJ243927142 (DE-600)2026349-1 1600-051X nnns volume:32 year:2005 number:7 pages:0 http://dx.doi.org/10.1111/j.1600-051X.2005.00740.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 2005 7 0 |
| allfieldsSound |
10.1111/j.1600-051X.2005.00740.x doi (DE-627)NLEJ243319800 DE-627 ger DE-627 rakwb Jervøe-Storm, P.-M. verfasserin aut Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real-time PCR with conventional anaerobic cultivation.Method: A total of 78 subgingival plaque samples were harvested from pockets <inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE740:ges" location="ges.gif"/>5 mm in 22 patients with advanced chronic periodontitis and immediately transferred into transport medium. Aliquots were evaluated with species-specific probes by real-time PCR (meridol® Perio Diagnostics, GABA) and anaerobic bacteria culture on selective media for the detection of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis. The analysis was performed by two separate, blinded examiners.Results: When real-time PCR was compared with the culture method (golden standard) for the detection of putative periodontopathogenic bacteria, the sensitivity and specificity for A. actinomycetemcomitans were 67% and 100%, respectively (κ: 0.79); for F. nucleatum 73% and 53%, respectively (κ: 0.21); for P. gingivalis 94% and 84%, respectively (κ: 0.77); for P. intermedia 33% and 94%, respectively (κ: 0.26) and for T. forsythensis 92% and 56%, respectively (κ: 0.51). Spearman's correlation coefficients for the quantitative results of both methods were 0.82 for A. actinomycetemcomitans, 0.33 for F. nucleatum, 0.83 for P. gingivalis, 0.38 for P. intermedia and 0.67 for T. forsythensis.Conclusion: Overall, the agreement between both test methods was excellent for A. actinomycetemcomitans and P. gingivalis, fair for T. forsythensis and poor for F. nucleatum and P. intermedia. The discrepancies in the results may be explained by the inability of cultivation methods to distinguish between close related taxa, and the problems of keeping periopathogenic bacteria viable, which is required for standard cultivation. 2005 Blackwell Publishing Journal Backfiles 1879-2005 |2005|||||||||| bacterial culture Koltzscher, M. verfasserin aut Falk, W. verfasserin aut Dörfler, A. oth Jepsen, S. oth In Journal of clinical periodontology Oxford [u.a.] : Wiley-Blackwell, 1974 32(2005), 7, Seite 0 Online-Ressource (DE-627)NLEJ243927142 (DE-600)2026349-1 1600-051X nnns volume:32 year:2005 number:7 pages:0 http://dx.doi.org/10.1111/j.1600-051X.2005.00740.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 2005 7 0 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ243319800</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20210707175508.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120427s2005 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/j.1600-051X.2005.00740.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ243319800</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Jervøe-Storm, P.-M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford, UK</subfield><subfield code="b">Munksgaard International Publishers</subfield><subfield code="c">2005</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real-time PCR with conventional anaerobic cultivation.Method: A total of 78 subgingival plaque samples were harvested from pockets <inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE740:ges" location="ges.gif"/>5 mm in 22 patients with advanced chronic periodontitis and immediately transferred into transport medium. Aliquots were evaluated with species-specific probes by real-time PCR (meridol® Perio Diagnostics, GABA) and anaerobic bacteria culture on selective media for the detection of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis. The analysis was performed by two separate, blinded examiners.Results: When real-time PCR was compared with the culture method (golden standard) for the detection of putative periodontopathogenic bacteria, the sensitivity and specificity for A. actinomycetemcomitans were 67% and 100%, respectively (κ: 0.79); for F. nucleatum 73% and 53%, respectively (κ: 0.21); for P. gingivalis 94% and 84%, respectively (κ: 0.77); for P. intermedia 33% and 94%, respectively (κ: 0.26) and for T. forsythensis 92% and 56%, respectively (κ: 0.51). Spearman's correlation coefficients for the quantitative results of both methods were 0.82 for A. actinomycetemcomitans, 0.33 for F. nucleatum, 0.83 for P. gingivalis, 0.38 for P. intermedia and 0.67 for T. forsythensis.Conclusion: Overall, the agreement between both test methods was excellent for A. actinomycetemcomitans and P. gingivalis, fair for T. forsythensis and poor for F. nucleatum and P. intermedia. The discrepancies in the results may be explained by the inability of cultivation methods to distinguish between close related taxa, and the problems of keeping periopathogenic bacteria viable, which is required for standard cultivation.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2005</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2005||||||||||</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">bacterial culture</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Koltzscher, M.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Falk, W.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Dörfler, A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jepsen, S.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of clinical periodontology</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1974</subfield><subfield code="g">32(2005), 7, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927142</subfield><subfield code="w">(DE-600)2026349-1</subfield><subfield code="x">1600-051X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:32</subfield><subfield code="g">year:2005</subfield><subfield code="g">number:7</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1111/j.1600-051X.2005.00740.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">32</subfield><subfield code="j">2005</subfield><subfield code="e">7</subfield><subfield code="h">0</subfield></datafield></record></collection>
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Jervøe-Storm, P.-M. |
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Jervøe-Storm, P.-M. misc bacterial culture Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples |
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Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples bacterial culture |
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comparison of culture and real-time pcr for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples |
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Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples |
| abstract |
Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real-time PCR with conventional anaerobic cultivation.Method: A total of 78 subgingival plaque samples were harvested from pockets <inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE740:ges" location="ges.gif"/>5 mm in 22 patients with advanced chronic periodontitis and immediately transferred into transport medium. Aliquots were evaluated with species-specific probes by real-time PCR (meridol® Perio Diagnostics, GABA) and anaerobic bacteria culture on selective media for the detection of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis. The analysis was performed by two separate, blinded examiners.Results: When real-time PCR was compared with the culture method (golden standard) for the detection of putative periodontopathogenic bacteria, the sensitivity and specificity for A. actinomycetemcomitans were 67% and 100%, respectively (κ: 0.79); for F. nucleatum 73% and 53%, respectively (κ: 0.21); for P. gingivalis 94% and 84%, respectively (κ: 0.77); for P. intermedia 33% and 94%, respectively (κ: 0.26) and for T. forsythensis 92% and 56%, respectively (κ: 0.51). Spearman's correlation coefficients for the quantitative results of both methods were 0.82 for A. actinomycetemcomitans, 0.33 for F. nucleatum, 0.83 for P. gingivalis, 0.38 for P. intermedia and 0.67 for T. forsythensis.Conclusion: Overall, the agreement between both test methods was excellent for A. actinomycetemcomitans and P. gingivalis, fair for T. forsythensis and poor for F. nucleatum and P. intermedia. The discrepancies in the results may be explained by the inability of cultivation methods to distinguish between close related taxa, and the problems of keeping periopathogenic bacteria viable, which is required for standard cultivation. |
| abstractGer |
Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real-time PCR with conventional anaerobic cultivation.Method: A total of 78 subgingival plaque samples were harvested from pockets <inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE740:ges" location="ges.gif"/>5 mm in 22 patients with advanced chronic periodontitis and immediately transferred into transport medium. Aliquots were evaluated with species-specific probes by real-time PCR (meridol® Perio Diagnostics, GABA) and anaerobic bacteria culture on selective media for the detection of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis. The analysis was performed by two separate, blinded examiners.Results: When real-time PCR was compared with the culture method (golden standard) for the detection of putative periodontopathogenic bacteria, the sensitivity and specificity for A. actinomycetemcomitans were 67% and 100%, respectively (κ: 0.79); for F. nucleatum 73% and 53%, respectively (κ: 0.21); for P. gingivalis 94% and 84%, respectively (κ: 0.77); for P. intermedia 33% and 94%, respectively (κ: 0.26) and for T. forsythensis 92% and 56%, respectively (κ: 0.51). Spearman's correlation coefficients for the quantitative results of both methods were 0.82 for A. actinomycetemcomitans, 0.33 for F. nucleatum, 0.83 for P. gingivalis, 0.38 for P. intermedia and 0.67 for T. forsythensis.Conclusion: Overall, the agreement between both test methods was excellent for A. actinomycetemcomitans and P. gingivalis, fair for T. forsythensis and poor for F. nucleatum and P. intermedia. The discrepancies in the results may be explained by the inability of cultivation methods to distinguish between close related taxa, and the problems of keeping periopathogenic bacteria viable, which is required for standard cultivation. |
| abstract_unstemmed |
Objectives: Bacterial cultivation is a well-established method for analyzing plaque samples. Real-time polymerase chain reaction (PCR) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. In this study, we compared real-time PCR with conventional anaerobic cultivation.Method: A total of 78 subgingival plaque samples were harvested from pockets <inlineGraphic alt="geqslant R: gt-or-equal, slanted" extraInfo="nonStandardEntity" href="urn:x-wiley:03036979:JCPE740:ges" location="ges.gif"/>5 mm in 22 patients with advanced chronic periodontitis and immediately transferred into transport medium. Aliquots were evaluated with species-specific probes by real-time PCR (meridol® Perio Diagnostics, GABA) and anaerobic bacteria culture on selective media for the detection of Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythensis. The analysis was performed by two separate, blinded examiners.Results: When real-time PCR was compared with the culture method (golden standard) for the detection of putative periodontopathogenic bacteria, the sensitivity and specificity for A. actinomycetemcomitans were 67% and 100%, respectively (κ: 0.79); for F. nucleatum 73% and 53%, respectively (κ: 0.21); for P. gingivalis 94% and 84%, respectively (κ: 0.77); for P. intermedia 33% and 94%, respectively (κ: 0.26) and for T. forsythensis 92% and 56%, respectively (κ: 0.51). Spearman's correlation coefficients for the quantitative results of both methods were 0.82 for A. actinomycetemcomitans, 0.33 for F. nucleatum, 0.83 for P. gingivalis, 0.38 for P. intermedia and 0.67 for T. forsythensis.Conclusion: Overall, the agreement between both test methods was excellent for A. actinomycetemcomitans and P. gingivalis, fair for T. forsythensis and poor for F. nucleatum and P. intermedia. The discrepancies in the results may be explained by the inability of cultivation methods to distinguish between close related taxa, and the problems of keeping periopathogenic bacteria viable, which is required for standard cultivation. |
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Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples |
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