Triclosan reduces microsomal prostaglandin E synthase-1 expression in human gingival fibroblasts
Objective: The effect of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) on the expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and on the translocation of the nuclear factor-κB (NF-κB) in relation to prostaglandin E2 (PGE2) production was investigate...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Mustafa, M. [verfasserIn] Wondimu, B. [verfasserIn] Yucel-Lindberg, T. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Munksgaard International Publishers ; 2005 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2004 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Journal of clinical periodontology - Oxford [u.a.] : Wiley-Blackwell, 1974, 32(2005), 1, Seite 0 |
| Übergeordnetes Werk: |
volume:32 ; year:2005 ; number:1 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1111/j.1600-051X.2004.00622.x |
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| Katalog-ID: |
NLEJ243321295 |
|---|
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| 245 | 1 | 0 | |a Triclosan reduces microsomal prostaglandin E synthase-1 expression in human gingival fibroblasts |
| 264 | 1 | |a Oxford, UK |b Munksgaard International Publishers |c 2005 | |
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| 520 | |a Objective: The effect of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) on the expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and on the translocation of the nuclear factor-κB (NF-κB) in relation to prostaglandin E2 (PGE2) production was investigated in human gingival fibroblasts challenged with tumor necrosis factor α (TNFα).Methods: Fibroblasts were established from gingival biopsies obtained from six children. COX-2 mRNA and protein expression was quantified using mRNA quantitation and enzyme immunometric assay kits. mPGES-1 mRNA was analysed by RT-PCR, mPGES-1 protein and NF-κB translocation by immunoblotting. PGE2 was determined by radioimmunoassay.Results: The cytokine TNFα enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts. Treatment of gingival fibroblasts with triclosan (1 μg/ml) significantly reduced the stimulatory effect of TNFα (10 ng/ml) on the expression of mPGES-1 at both the mRNA and the protein level by an average of 21% and 43%, respectively, and subsequently the production of PGE2 (p<0.01). Triclosan did not, however, affect the translocation of NF-κB or the expression of COX-2 in TNFα-stimulated cells.Conclusion: The results show that triclosan reduces the augmented biosynthesis of PGE2 by inhibiting the mRNA and the protein expression of mPGES-1 in gingival fibroblasts. This finding may partly explain the anti-inflammatory effect of the agent previously reported in clinical studies. | ||
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| 700 | 1 | |a Yucel-Lindberg, T. |e verfasserin |4 aut | |
| 700 | 1 | |a Kats-Hallström, A. T. |4 oth | |
| 700 | 1 | |a Jonsson, A. S. |4 oth | |
| 700 | 1 | |a Modéer, T. |4 oth | |
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10.1111/j.1600-051X.2004.00622.x doi (DE-627)NLEJ243321295 DE-627 ger DE-627 rakwb Mustafa, M. verfasserin aut Triclosan reduces microsomal prostaglandin E synthase-1 expression in human gingival fibroblasts Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Objective: The effect of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) on the expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and on the translocation of the nuclear factor-κB (NF-κB) in relation to prostaglandin E2 (PGE2) production was investigated in human gingival fibroblasts challenged with tumor necrosis factor α (TNFα).Methods: Fibroblasts were established from gingival biopsies obtained from six children. COX-2 mRNA and protein expression was quantified using mRNA quantitation and enzyme immunometric assay kits. mPGES-1 mRNA was analysed by RT-PCR, mPGES-1 protein and NF-κB translocation by immunoblotting. PGE2 was determined by radioimmunoassay.Results: The cytokine TNFα enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts. Treatment of gingival fibroblasts with triclosan (1 μg/ml) significantly reduced the stimulatory effect of TNFα (10 ng/ml) on the expression of mPGES-1 at both the mRNA and the protein level by an average of 21% and 43%, respectively, and subsequently the production of PGE2 (p<0.01). Triclosan did not, however, affect the translocation of NF-κB or the expression of COX-2 in TNFα-stimulated cells.Conclusion: The results show that triclosan reduces the augmented biosynthesis of PGE2 by inhibiting the mRNA and the protein expression of mPGES-1 in gingival fibroblasts. This finding may partly explain the anti-inflammatory effect of the agent previously reported in clinical studies. 2004 Blackwell Publishing Journal Backfiles 1879-2005 |2004|||||||||| gingival fibroblasts Wondimu, B. verfasserin aut Yucel-Lindberg, T. verfasserin aut Kats-Hallström, A. T. oth Jonsson, A. S. oth Modéer, T. oth In Journal of clinical periodontology Oxford [u.a.] : Wiley-Blackwell, 1974 32(2005), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927142 (DE-600)2026349-1 1600-051X nnns volume:32 year:2005 number:1 pages:0 http://dx.doi.org/10.1111/j.1600-051X.2004.00622.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 2005 1 0 |
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10.1111/j.1600-051X.2004.00622.x doi (DE-627)NLEJ243321295 DE-627 ger DE-627 rakwb Mustafa, M. verfasserin aut Triclosan reduces microsomal prostaglandin E synthase-1 expression in human gingival fibroblasts Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Objective: The effect of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) on the expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and on the translocation of the nuclear factor-κB (NF-κB) in relation to prostaglandin E2 (PGE2) production was investigated in human gingival fibroblasts challenged with tumor necrosis factor α (TNFα).Methods: Fibroblasts were established from gingival biopsies obtained from six children. COX-2 mRNA and protein expression was quantified using mRNA quantitation and enzyme immunometric assay kits. mPGES-1 mRNA was analysed by RT-PCR, mPGES-1 protein and NF-κB translocation by immunoblotting. PGE2 was determined by radioimmunoassay.Results: The cytokine TNFα enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts. Treatment of gingival fibroblasts with triclosan (1 μg/ml) significantly reduced the stimulatory effect of TNFα (10 ng/ml) on the expression of mPGES-1 at both the mRNA and the protein level by an average of 21% and 43%, respectively, and subsequently the production of PGE2 (p<0.01). Triclosan did not, however, affect the translocation of NF-κB or the expression of COX-2 in TNFα-stimulated cells.Conclusion: The results show that triclosan reduces the augmented biosynthesis of PGE2 by inhibiting the mRNA and the protein expression of mPGES-1 in gingival fibroblasts. This finding may partly explain the anti-inflammatory effect of the agent previously reported in clinical studies. 2004 Blackwell Publishing Journal Backfiles 1879-2005 |2004|||||||||| gingival fibroblasts Wondimu, B. verfasserin aut Yucel-Lindberg, T. verfasserin aut Kats-Hallström, A. T. oth Jonsson, A. S. oth Modéer, T. oth In Journal of clinical periodontology Oxford [u.a.] : Wiley-Blackwell, 1974 32(2005), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927142 (DE-600)2026349-1 1600-051X nnns volume:32 year:2005 number:1 pages:0 http://dx.doi.org/10.1111/j.1600-051X.2004.00622.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 2005 1 0 |
| allfields_unstemmed |
10.1111/j.1600-051X.2004.00622.x doi (DE-627)NLEJ243321295 DE-627 ger DE-627 rakwb Mustafa, M. verfasserin aut Triclosan reduces microsomal prostaglandin E synthase-1 expression in human gingival fibroblasts Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Objective: The effect of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) on the expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and on the translocation of the nuclear factor-κB (NF-κB) in relation to prostaglandin E2 (PGE2) production was investigated in human gingival fibroblasts challenged with tumor necrosis factor α (TNFα).Methods: Fibroblasts were established from gingival biopsies obtained from six children. COX-2 mRNA and protein expression was quantified using mRNA quantitation and enzyme immunometric assay kits. mPGES-1 mRNA was analysed by RT-PCR, mPGES-1 protein and NF-κB translocation by immunoblotting. PGE2 was determined by radioimmunoassay.Results: The cytokine TNFα enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts. Treatment of gingival fibroblasts with triclosan (1 μg/ml) significantly reduced the stimulatory effect of TNFα (10 ng/ml) on the expression of mPGES-1 at both the mRNA and the protein level by an average of 21% and 43%, respectively, and subsequently the production of PGE2 (p<0.01). Triclosan did not, however, affect the translocation of NF-κB or the expression of COX-2 in TNFα-stimulated cells.Conclusion: The results show that triclosan reduces the augmented biosynthesis of PGE2 by inhibiting the mRNA and the protein expression of mPGES-1 in gingival fibroblasts. This finding may partly explain the anti-inflammatory effect of the agent previously reported in clinical studies. 2004 Blackwell Publishing Journal Backfiles 1879-2005 |2004|||||||||| gingival fibroblasts Wondimu, B. verfasserin aut Yucel-Lindberg, T. verfasserin aut Kats-Hallström, A. T. oth Jonsson, A. S. oth Modéer, T. oth In Journal of clinical periodontology Oxford [u.a.] : Wiley-Blackwell, 1974 32(2005), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927142 (DE-600)2026349-1 1600-051X nnns volume:32 year:2005 number:1 pages:0 http://dx.doi.org/10.1111/j.1600-051X.2004.00622.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 2005 1 0 |
| allfieldsGer |
10.1111/j.1600-051X.2004.00622.x doi (DE-627)NLEJ243321295 DE-627 ger DE-627 rakwb Mustafa, M. verfasserin aut Triclosan reduces microsomal prostaglandin E synthase-1 expression in human gingival fibroblasts Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Objective: The effect of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) on the expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and on the translocation of the nuclear factor-κB (NF-κB) in relation to prostaglandin E2 (PGE2) production was investigated in human gingival fibroblasts challenged with tumor necrosis factor α (TNFα).Methods: Fibroblasts were established from gingival biopsies obtained from six children. COX-2 mRNA and protein expression was quantified using mRNA quantitation and enzyme immunometric assay kits. mPGES-1 mRNA was analysed by RT-PCR, mPGES-1 protein and NF-κB translocation by immunoblotting. PGE2 was determined by radioimmunoassay.Results: The cytokine TNFα enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts. Treatment of gingival fibroblasts with triclosan (1 μg/ml) significantly reduced the stimulatory effect of TNFα (10 ng/ml) on the expression of mPGES-1 at both the mRNA and the protein level by an average of 21% and 43%, respectively, and subsequently the production of PGE2 (p<0.01). Triclosan did not, however, affect the translocation of NF-κB or the expression of COX-2 in TNFα-stimulated cells.Conclusion: The results show that triclosan reduces the augmented biosynthesis of PGE2 by inhibiting the mRNA and the protein expression of mPGES-1 in gingival fibroblasts. This finding may partly explain the anti-inflammatory effect of the agent previously reported in clinical studies. 2004 Blackwell Publishing Journal Backfiles 1879-2005 |2004|||||||||| gingival fibroblasts Wondimu, B. verfasserin aut Yucel-Lindberg, T. verfasserin aut Kats-Hallström, A. T. oth Jonsson, A. S. oth Modéer, T. oth In Journal of clinical periodontology Oxford [u.a.] : Wiley-Blackwell, 1974 32(2005), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927142 (DE-600)2026349-1 1600-051X nnns volume:32 year:2005 number:1 pages:0 http://dx.doi.org/10.1111/j.1600-051X.2004.00622.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 2005 1 0 |
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10.1111/j.1600-051X.2004.00622.x doi (DE-627)NLEJ243321295 DE-627 ger DE-627 rakwb Mustafa, M. verfasserin aut Triclosan reduces microsomal prostaglandin E synthase-1 expression in human gingival fibroblasts Oxford, UK Munksgaard International Publishers 2005 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Objective: The effect of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) on the expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and on the translocation of the nuclear factor-κB (NF-κB) in relation to prostaglandin E2 (PGE2) production was investigated in human gingival fibroblasts challenged with tumor necrosis factor α (TNFα).Methods: Fibroblasts were established from gingival biopsies obtained from six children. COX-2 mRNA and protein expression was quantified using mRNA quantitation and enzyme immunometric assay kits. mPGES-1 mRNA was analysed by RT-PCR, mPGES-1 protein and NF-κB translocation by immunoblotting. PGE2 was determined by radioimmunoassay.Results: The cytokine TNFα enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts. Treatment of gingival fibroblasts with triclosan (1 μg/ml) significantly reduced the stimulatory effect of TNFα (10 ng/ml) on the expression of mPGES-1 at both the mRNA and the protein level by an average of 21% and 43%, respectively, and subsequently the production of PGE2 (p<0.01). Triclosan did not, however, affect the translocation of NF-κB or the expression of COX-2 in TNFα-stimulated cells.Conclusion: The results show that triclosan reduces the augmented biosynthesis of PGE2 by inhibiting the mRNA and the protein expression of mPGES-1 in gingival fibroblasts. This finding may partly explain the anti-inflammatory effect of the agent previously reported in clinical studies. 2004 Blackwell Publishing Journal Backfiles 1879-2005 |2004|||||||||| gingival fibroblasts Wondimu, B. verfasserin aut Yucel-Lindberg, T. verfasserin aut Kats-Hallström, A. T. oth Jonsson, A. S. oth Modéer, T. oth In Journal of clinical periodontology Oxford [u.a.] : Wiley-Blackwell, 1974 32(2005), 1, Seite 0 Online-Ressource (DE-627)NLEJ243927142 (DE-600)2026349-1 1600-051X nnns volume:32 year:2005 number:1 pages:0 http://dx.doi.org/10.1111/j.1600-051X.2004.00622.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 32 2005 1 0 |
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Mustafa, M. @@aut@@ Wondimu, B. @@aut@@ Yucel-Lindberg, T. @@aut@@ Kats-Hallström, A. T. @@oth@@ Jonsson, A. S. @@oth@@ Modéer, T. @@oth@@ |
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COX-2 mRNA and protein expression was quantified using mRNA quantitation and enzyme immunometric assay kits. mPGES-1 mRNA was analysed by RT-PCR, mPGES-1 protein and NF-κB translocation by immunoblotting. PGE2 was determined by radioimmunoassay.Results: The cytokine TNFα enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts. Treatment of gingival fibroblasts with triclosan (1 μg/ml) significantly reduced the stimulatory effect of TNFα (10 ng/ml) on the expression of mPGES-1 at both the mRNA and the protein level by an average of 21% and 43%, respectively, and subsequently the production of PGE2 (p<0.01). Triclosan did not, however, affect the translocation of NF-κB or the expression of COX-2 in TNFα-stimulated cells.Conclusion: The results show that triclosan reduces the augmented biosynthesis of PGE2 by inhibiting the mRNA and the protein expression of mPGES-1 in gingival fibroblasts. 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Triclosan reduces microsomal prostaglandin E synthase-1 expression in human gingival fibroblasts |
| author_sort |
Mustafa, M. |
| journal |
Journal of clinical periodontology |
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Journal of clinical periodontology |
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2005 |
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Mustafa, M. Wondimu, B. Yucel-Lindberg, T. |
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32 |
| physical |
Online-Ressource |
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Elektronische Aufsätze |
| author-letter |
Mustafa, M. |
| doi_str_mv |
10.1111/j.1600-051X.2004.00622.x |
| author2-role |
verfasserin |
| title_sort |
triclosan reduces microsomal prostaglandin e synthase-1 expression in human gingival fibroblasts |
| title_auth |
Triclosan reduces microsomal prostaglandin E synthase-1 expression in human gingival fibroblasts |
| abstract |
Objective: The effect of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) on the expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and on the translocation of the nuclear factor-κB (NF-κB) in relation to prostaglandin E2 (PGE2) production was investigated in human gingival fibroblasts challenged with tumor necrosis factor α (TNFα).Methods: Fibroblasts were established from gingival biopsies obtained from six children. COX-2 mRNA and protein expression was quantified using mRNA quantitation and enzyme immunometric assay kits. mPGES-1 mRNA was analysed by RT-PCR, mPGES-1 protein and NF-κB translocation by immunoblotting. PGE2 was determined by radioimmunoassay.Results: The cytokine TNFα enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts. Treatment of gingival fibroblasts with triclosan (1 μg/ml) significantly reduced the stimulatory effect of TNFα (10 ng/ml) on the expression of mPGES-1 at both the mRNA and the protein level by an average of 21% and 43%, respectively, and subsequently the production of PGE2 (p<0.01). Triclosan did not, however, affect the translocation of NF-κB or the expression of COX-2 in TNFα-stimulated cells.Conclusion: The results show that triclosan reduces the augmented biosynthesis of PGE2 by inhibiting the mRNA and the protein expression of mPGES-1 in gingival fibroblasts. This finding may partly explain the anti-inflammatory effect of the agent previously reported in clinical studies. |
| abstractGer |
Objective: The effect of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) on the expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and on the translocation of the nuclear factor-κB (NF-κB) in relation to prostaglandin E2 (PGE2) production was investigated in human gingival fibroblasts challenged with tumor necrosis factor α (TNFα).Methods: Fibroblasts were established from gingival biopsies obtained from six children. COX-2 mRNA and protein expression was quantified using mRNA quantitation and enzyme immunometric assay kits. mPGES-1 mRNA was analysed by RT-PCR, mPGES-1 protein and NF-κB translocation by immunoblotting. PGE2 was determined by radioimmunoassay.Results: The cytokine TNFα enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts. Treatment of gingival fibroblasts with triclosan (1 μg/ml) significantly reduced the stimulatory effect of TNFα (10 ng/ml) on the expression of mPGES-1 at both the mRNA and the protein level by an average of 21% and 43%, respectively, and subsequently the production of PGE2 (p<0.01). Triclosan did not, however, affect the translocation of NF-κB or the expression of COX-2 in TNFα-stimulated cells.Conclusion: The results show that triclosan reduces the augmented biosynthesis of PGE2 by inhibiting the mRNA and the protein expression of mPGES-1 in gingival fibroblasts. This finding may partly explain the anti-inflammatory effect of the agent previously reported in clinical studies. |
| abstract_unstemmed |
Objective: The effect of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) on the expression of cyclooxygenase-2 (COX-2) and microsomal prostaglandin E synthase-1 (mPGES-1) and on the translocation of the nuclear factor-κB (NF-κB) in relation to prostaglandin E2 (PGE2) production was investigated in human gingival fibroblasts challenged with tumor necrosis factor α (TNFα).Methods: Fibroblasts were established from gingival biopsies obtained from six children. COX-2 mRNA and protein expression was quantified using mRNA quantitation and enzyme immunometric assay kits. mPGES-1 mRNA was analysed by RT-PCR, mPGES-1 protein and NF-κB translocation by immunoblotting. PGE2 was determined by radioimmunoassay.Results: The cytokine TNFα enhanced the expression of mRNA as well as the protein levels of both COX-2 and mPGES-1 and subsequently the production of PGE2 in gingival fibroblasts. Treatment of gingival fibroblasts with triclosan (1 μg/ml) significantly reduced the stimulatory effect of TNFα (10 ng/ml) on the expression of mPGES-1 at both the mRNA and the protein level by an average of 21% and 43%, respectively, and subsequently the production of PGE2 (p<0.01). Triclosan did not, however, affect the translocation of NF-κB or the expression of COX-2 in TNFα-stimulated cells.Conclusion: The results show that triclosan reduces the augmented biosynthesis of PGE2 by inhibiting the mRNA and the protein expression of mPGES-1 in gingival fibroblasts. This finding may partly explain the anti-inflammatory effect of the agent previously reported in clinical studies. |
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| title_short |
Triclosan reduces microsomal prostaglandin E synthase-1 expression in human gingival fibroblasts |
| url |
http://dx.doi.org/10.1111/j.1600-051X.2004.00622.x |
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| author2 |
Wondimu, B. Yucel-Lindberg, T. Kats-Hallström, A. T. Jonsson, A. S. Modéer, T. |
| author2Str |
Wondimu, B. Yucel-Lindberg, T. Kats-Hallström, A. T. Jonsson, A. S. Modéer, T. |
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| up_date |
2024-07-06T05:04:11.003Z |
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