Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth
Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Coers, Jörn [verfasserIn] Kagan, Jonathan C. [verfasserIn] Matthews, Miguelina [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Science, Ltd ; 2000 |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2002 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Molecular microbiology - Oxford [u.a.] : Wiley-Blackwell, 1987, 38(2000), 4, Seite 0 |
| Übergeordnetes Werk: |
volume:38 ; year:2000 ; number:4 ; pages:0 |
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| DOI / URN: |
10.1046/j.1365-2958.2000.02176.x |
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| 520 | |a Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 dot and icm genes, which encode a type IV-related transport apparatus. To understand how this transporter functions, isogenic L. pneumophila dot and icm mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein–protein interactions. Specific interactions between different Icm proteins were detected by yeast two-hybrid and gel overlay analysis. These data support a model in which the IcmQ–IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS–IcmW complex is required for export of virulence determinants that modulate phagosome trafficking. | ||
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10.1046/j.1365-2958.2000.02176.x doi (DE-627)NLEJ243565291 DE-627 ger DE-627 rakwb Coers, Jörn verfasserin aut Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth Oxford, UK Blackwell Science, Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 dot and icm genes, which encode a type IV-related transport apparatus. To understand how this transporter functions, isogenic L. pneumophila dot and icm mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein–protein interactions. Specific interactions between different Icm proteins were detected by yeast two-hybrid and gel overlay analysis. These data support a model in which the IcmQ–IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS–IcmW complex is required for export of virulence determinants that modulate phagosome trafficking. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Kagan, Jonathan C. verfasserin aut Matthews, Miguelina verfasserin aut Nagai, Hiroki oth Zuckman, Deborah M. oth Roy, Craig R. oth In Molecular microbiology Oxford [u.a.] : Wiley-Blackwell, 1987 38(2000), 4, Seite 0 Online-Ressource (DE-627)NLEJ243926537 (DE-600)1501537-3 1365-2958 nnns volume:38 year:2000 number:4 pages:0 http://dx.doi.org/10.1046/j.1365-2958.2000.02176.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 38 2000 4 0 |
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10.1046/j.1365-2958.2000.02176.x doi (DE-627)NLEJ243565291 DE-627 ger DE-627 rakwb Coers, Jörn verfasserin aut Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth Oxford, UK Blackwell Science, Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 dot and icm genes, which encode a type IV-related transport apparatus. To understand how this transporter functions, isogenic L. pneumophila dot and icm mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein–protein interactions. Specific interactions between different Icm proteins were detected by yeast two-hybrid and gel overlay analysis. These data support a model in which the IcmQ–IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS–IcmW complex is required for export of virulence determinants that modulate phagosome trafficking. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Kagan, Jonathan C. verfasserin aut Matthews, Miguelina verfasserin aut Nagai, Hiroki oth Zuckman, Deborah M. oth Roy, Craig R. oth In Molecular microbiology Oxford [u.a.] : Wiley-Blackwell, 1987 38(2000), 4, Seite 0 Online-Ressource (DE-627)NLEJ243926537 (DE-600)1501537-3 1365-2958 nnns volume:38 year:2000 number:4 pages:0 http://dx.doi.org/10.1046/j.1365-2958.2000.02176.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 38 2000 4 0 |
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10.1046/j.1365-2958.2000.02176.x doi (DE-627)NLEJ243565291 DE-627 ger DE-627 rakwb Coers, Jörn verfasserin aut Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth Oxford, UK Blackwell Science, Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 dot and icm genes, which encode a type IV-related transport apparatus. To understand how this transporter functions, isogenic L. pneumophila dot and icm mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein–protein interactions. Specific interactions between different Icm proteins were detected by yeast two-hybrid and gel overlay analysis. These data support a model in which the IcmQ–IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS–IcmW complex is required for export of virulence determinants that modulate phagosome trafficking. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Kagan, Jonathan C. verfasserin aut Matthews, Miguelina verfasserin aut Nagai, Hiroki oth Zuckman, Deborah M. oth Roy, Craig R. oth In Molecular microbiology Oxford [u.a.] : Wiley-Blackwell, 1987 38(2000), 4, Seite 0 Online-Ressource (DE-627)NLEJ243926537 (DE-600)1501537-3 1365-2958 nnns volume:38 year:2000 number:4 pages:0 http://dx.doi.org/10.1046/j.1365-2958.2000.02176.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 38 2000 4 0 |
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10.1046/j.1365-2958.2000.02176.x doi (DE-627)NLEJ243565291 DE-627 ger DE-627 rakwb Coers, Jörn verfasserin aut Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth Oxford, UK Blackwell Science, Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 dot and icm genes, which encode a type IV-related transport apparatus. To understand how this transporter functions, isogenic L. pneumophila dot and icm mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein–protein interactions. Specific interactions between different Icm proteins were detected by yeast two-hybrid and gel overlay analysis. These data support a model in which the IcmQ–IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS–IcmW complex is required for export of virulence determinants that modulate phagosome trafficking. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Kagan, Jonathan C. verfasserin aut Matthews, Miguelina verfasserin aut Nagai, Hiroki oth Zuckman, Deborah M. oth Roy, Craig R. oth In Molecular microbiology Oxford [u.a.] : Wiley-Blackwell, 1987 38(2000), 4, Seite 0 Online-Ressource (DE-627)NLEJ243926537 (DE-600)1501537-3 1365-2958 nnns volume:38 year:2000 number:4 pages:0 http://dx.doi.org/10.1046/j.1365-2958.2000.02176.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 38 2000 4 0 |
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10.1046/j.1365-2958.2000.02176.x doi (DE-627)NLEJ243565291 DE-627 ger DE-627 rakwb Coers, Jörn verfasserin aut Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth Oxford, UK Blackwell Science, Ltd 2000 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 dot and icm genes, which encode a type IV-related transport apparatus. To understand how this transporter functions, isogenic L. pneumophila dot and icm mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein–protein interactions. Specific interactions between different Icm proteins were detected by yeast two-hybrid and gel overlay analysis. These data support a model in which the IcmQ–IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS–IcmW complex is required for export of virulence determinants that modulate phagosome trafficking. 2002 Blackwell Publishing Journal Backfiles 1879-2005 |2002|||||||||| Kagan, Jonathan C. verfasserin aut Matthews, Miguelina verfasserin aut Nagai, Hiroki oth Zuckman, Deborah M. oth Roy, Craig R. oth In Molecular microbiology Oxford [u.a.] : Wiley-Blackwell, 1987 38(2000), 4, Seite 0 Online-Ressource (DE-627)NLEJ243926537 (DE-600)1501537-3 1365-2958 nnns volume:38 year:2000 number:4 pages:0 http://dx.doi.org/10.1046/j.1365-2958.2000.02176.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 38 2000 4 0 |
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identification of icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for legionella pneumophila intracellular growth |
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Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth |
| abstract |
Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 dot and icm genes, which encode a type IV-related transport apparatus. To understand how this transporter functions, isogenic L. pneumophila dot and icm mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein–protein interactions. Specific interactions between different Icm proteins were detected by yeast two-hybrid and gel overlay analysis. These data support a model in which the IcmQ–IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS–IcmW complex is required for export of virulence determinants that modulate phagosome trafficking. |
| abstractGer |
Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 dot and icm genes, which encode a type IV-related transport apparatus. To understand how this transporter functions, isogenic L. pneumophila dot and icm mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein–protein interactions. Specific interactions between different Icm proteins were detected by yeast two-hybrid and gel overlay analysis. These data support a model in which the IcmQ–IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS–IcmW complex is required for export of virulence determinants that modulate phagosome trafficking. |
| abstract_unstemmed |
Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 dot and icm genes, which encode a type IV-related transport apparatus. To understand how this transporter functions, isogenic L. pneumophila dot and icm mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein–protein interactions. Specific interactions between different Icm proteins were detected by yeast two-hybrid and gel overlay analysis. These data support a model in which the IcmQ–IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS–IcmW complex is required for export of virulence determinants that modulate phagosome trafficking. |
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Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth |
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Kagan, Jonathan C. Matthews, Miguelina Nagai, Hiroki Zuckman, Deborah M. Roy, Craig R. |
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Kagan, Jonathan C. Matthews, Miguelina Nagai, Hiroki Zuckman, Deborah M. Roy, Craig R. |
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NLEJ243926537 |
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10.1046/j.1365-2958.2000.02176.x |
| up_date |
2024-07-06T05:51:52.457Z |
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