Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment
Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites....
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Madrid, Enrique Alberto [verfasserIn] Laxalt, Ana María [verfasserIn] Beligni, Verónica [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|
| Erschienen: |
Copenhagen: Munksgaard International Publishers ; 1999 |
|---|
| Umfang: |
Online-Ressource |
|---|
| Reproduktion: |
2008 ; Blackwell Publishing Journal Backfiles 1879-2005 |
|---|---|
| Übergeordnetes Werk: |
In: Physiologia plantarum - Oxford [u.a.] : Wiley-Blackwell, 1948, 105(1999), 2, Seite 0 |
| Übergeordnetes Werk: |
volume:105 ; year:1999 ; number:2 ; pages:0 |
| Links: |
|---|
| DOI / URN: |
10.1034/j.1399-3054.1999.105216.x |
|---|
| Katalog-ID: |
NLEJ24378872X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ24378872X | ||
| 003 | DE-627 | ||
| 005 | 20230505222518.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 120427s1999 xx |||||o 00| ||und c | ||
| 024 | 7 | |a 10.1034/j.1399-3054.1999.105216.x |2 doi | |
| 035 | |a (DE-627)NLEJ24378872X | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 100 | 1 | |a Madrid, Enrique Alberto |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment |
| 264 | 1 | |a Copenhagen |b Munksgaard International Publishers |c 1999 | |
| 300 | |a Online-Ressource | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants. | ||
| 533 | |d 2008 |f Blackwell Publishing Journal Backfiles 1879-2005 |7 |2008|||||||||| | ||
| 700 | 1 | |a Laxalt, Ana María |e verfasserin |4 aut | |
| 700 | 1 | |a Beligni, Verónica |e verfasserin |4 aut | |
| 700 | 1 | |a Lamattina, Lorenzo |4 oth | |
| 773 | 0 | 8 | |i In |t Physiologia plantarum |d Oxford [u.a.] : Wiley-Blackwell, 1948 |g 105(1999), 2, Seite 0 |h Online-Ressource |w (DE-627)NLEJ243927738 |w (DE-600)2020837-6 |x 1399-3054 |7 nnns |
| 773 | 1 | 8 | |g volume:105 |g year:1999 |g number:2 |g pages:0 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1034/j.1399-3054.1999.105216.x |q text/html |x Verlag |z Deutschlandweit zugänglich |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-DJB | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 105 |j 1999 |e 2 |h 0 | ||
| author_variant |
e a m ea eam a m l am aml v b vb |
|---|---|
| matchkey_str |
article:13993054:1999----::atacaatrztoopttffapssbntdaniseuainuigugl |
| hierarchy_sort_str |
1999 |
| publishDate |
1999 |
| allfields |
10.1034/j.1399-3054.1999.105216.x doi (DE-627)NLEJ24378872X DE-627 ger DE-627 rakwb Madrid, Enrique Alberto verfasserin aut Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment Copenhagen Munksgaard International Publishers 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Laxalt, Ana María verfasserin aut Beligni, Verónica verfasserin aut Lamattina, Lorenzo oth In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 105(1999), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:105 year:1999 number:2 pages:0 http://dx.doi.org/10.1034/j.1399-3054.1999.105216.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 105 1999 2 0 |
| spelling |
10.1034/j.1399-3054.1999.105216.x doi (DE-627)NLEJ24378872X DE-627 ger DE-627 rakwb Madrid, Enrique Alberto verfasserin aut Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment Copenhagen Munksgaard International Publishers 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Laxalt, Ana María verfasserin aut Beligni, Verónica verfasserin aut Lamattina, Lorenzo oth In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 105(1999), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:105 year:1999 number:2 pages:0 http://dx.doi.org/10.1034/j.1399-3054.1999.105216.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 105 1999 2 0 |
| allfields_unstemmed |
10.1034/j.1399-3054.1999.105216.x doi (DE-627)NLEJ24378872X DE-627 ger DE-627 rakwb Madrid, Enrique Alberto verfasserin aut Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment Copenhagen Munksgaard International Publishers 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Laxalt, Ana María verfasserin aut Beligni, Verónica verfasserin aut Lamattina, Lorenzo oth In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 105(1999), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:105 year:1999 number:2 pages:0 http://dx.doi.org/10.1034/j.1399-3054.1999.105216.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 105 1999 2 0 |
| allfieldsGer |
10.1034/j.1399-3054.1999.105216.x doi (DE-627)NLEJ24378872X DE-627 ger DE-627 rakwb Madrid, Enrique Alberto verfasserin aut Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment Copenhagen Munksgaard International Publishers 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Laxalt, Ana María verfasserin aut Beligni, Verónica verfasserin aut Lamattina, Lorenzo oth In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 105(1999), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:105 year:1999 number:2 pages:0 http://dx.doi.org/10.1034/j.1399-3054.1999.105216.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 105 1999 2 0 |
| allfieldsSound |
10.1034/j.1399-3054.1999.105216.x doi (DE-627)NLEJ24378872X DE-627 ger DE-627 rakwb Madrid, Enrique Alberto verfasserin aut Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment Copenhagen Munksgaard International Publishers 1999 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Laxalt, Ana María verfasserin aut Beligni, Verónica verfasserin aut Lamattina, Lorenzo oth In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 105(1999), 2, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:105 year:1999 number:2 pages:0 http://dx.doi.org/10.1034/j.1399-3054.1999.105216.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 105 1999 2 0 |
| source |
In Physiologia plantarum 105(1999), 2, Seite 0 volume:105 year:1999 number:2 pages:0 |
| sourceStr |
In Physiologia plantarum 105(1999), 2, Seite 0 volume:105 year:1999 number:2 pages:0 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
Physiologia plantarum |
| authorswithroles_txt_mv |
Madrid, Enrique Alberto @@aut@@ Laxalt, Ana María @@aut@@ Beligni, Verónica @@aut@@ Lamattina, Lorenzo @@oth@@ |
| publishDateDaySort_date |
1999-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ243927738 |
| id |
NLEJ24378872X |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ24378872X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230505222518.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120427s1999 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1034/j.1399-3054.1999.105216.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ24378872X</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Madrid, Enrique Alberto</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Copenhagen</subfield><subfield code="b">Munksgaard International Publishers</subfield><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2008</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2008||||||||||</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Laxalt, Ana María</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Beligni, Verónica</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lamattina, Lorenzo</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Physiologia plantarum</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1948</subfield><subfield code="g">105(1999), 2, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927738</subfield><subfield code="w">(DE-600)2020837-6</subfield><subfield code="x">1399-3054</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:105</subfield><subfield code="g">year:1999</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1034/j.1399-3054.1999.105216.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">105</subfield><subfield code="j">1999</subfield><subfield code="e">2</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| series2 |
Blackwell Publishing Journal Backfiles 1879-2005 |
| author |
Madrid, Enrique Alberto |
| spellingShingle |
Madrid, Enrique Alberto Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment |
| authorStr |
Madrid, Enrique Alberto |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ243927738 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| author_role |
aut aut aut |
| collection |
NL |
| publishPlace |
Copenhagen |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1399-3054 |
| topic_title |
Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment |
| publisher |
Munksgaard International Publishers |
| publisherStr |
Munksgaard International Publishers |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
l l ll |
| hierarchy_parent_title |
Physiologia plantarum |
| hierarchy_parent_id |
NLEJ243927738 |
| hierarchy_top_title |
Physiologia plantarum |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ243927738 (DE-600)2020837-6 |
| title |
Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment |
| ctrlnum |
(DE-627)NLEJ24378872X |
| title_full |
Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment |
| author_sort |
Madrid, Enrique Alberto |
| journal |
Physiologia plantarum |
| journalStr |
Physiologia plantarum |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
1999 |
| contenttype_str_mv |
zzz |
| container_start_page |
0 |
| author_browse |
Madrid, Enrique Alberto Laxalt, Ana María Beligni, Verónica |
| container_volume |
105 |
| physical |
Online-Ressource |
| format_se |
Elektronische Aufsätze |
| author-letter |
Madrid, Enrique Alberto |
| doi_str_mv |
10.1034/j.1399-3054.1999.105216.x |
| author2-role |
verfasserin |
| title_sort |
partial characterization of potato f0f1-atpase γ-subunit cdna and its regulation during fungal infection and elicitor treatment |
| title_auth |
Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment |
| abstract |
Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants. |
| abstractGer |
Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants. |
| abstract_unstemmed |
Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants. |
| collection_details |
GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE |
| container_issue |
2 |
| title_short |
Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment |
| url |
http://dx.doi.org/10.1034/j.1399-3054.1999.105216.x |
| remote_bool |
true |
| author2 |
Laxalt, Ana María Beligni, Verónica Lamattina, Lorenzo |
| author2Str |
Laxalt, Ana María Beligni, Verónica Lamattina, Lorenzo |
| ppnlink |
NLEJ243927738 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth |
| doi_str |
10.1034/j.1399-3054.1999.105216.x |
| up_date |
2024-07-06T06:32:40.971Z |
| _version_ |
1803810306493775872 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ24378872X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230505222518.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">120427s1999 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1034/j.1399-3054.1999.105216.x</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ24378872X</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Madrid, Enrique Alberto</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Partial characterization of potato F0F1-ATPase Γ-subunit cDNA and its regulation during fungal infection and elicitor treatment</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Copenhagen</subfield><subfield code="b">Munksgaard International Publishers</subfield><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Mitochondrial F0F1-ATP synthase from dicotyledonous plants contains six different subunits named α, β, Γ, Δ, Δ′ and ɛ. A key subunit in the coupling mechanism is the Γ-subunit, which appears as a single copy in the complex interacting with multiple α and β subunits that contain the catalytic sites. One 0.8-kb potato cDNA clone sharing 88% homology with the 3′-end of sweet potato Γ-ATPase has been isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification from potato leaves infected with the pathogenic fungus Phytophthora infestans. Northern blot experiments revealed that the potato Γ-subunit mRNA level increased 2- and 3-fold in infected potato leaves 48 and 72 h after infection, respectively. The enzyme activity of F0F1-ATPase was 2-fold higher in 48-h-infected potato leaves than in mock-inoculated ones. The fungal elicitors eicosapentanoic acid (EPA) and glucans from P. infestans cell wall were used in induction experiments on potato tuber discs. After 48 h of treatment, the Γ-ATPase mRNA levels were respectively increased by 1.8, 1.4 and 2.2-fold in EPA, glucans and EPA plus glucan treatments. The present results show that the Γ-subunit mRNA levels of F0F1-ATPase are up-regulated during biological stress conditions and elicitor treatments in potato and correlate with the increase of respiration rate and metabolic activity of mitochondria observed during infection processes in plants.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="d">2008</subfield><subfield code="f">Blackwell Publishing Journal Backfiles 1879-2005</subfield><subfield code="7">|2008||||||||||</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Laxalt, Ana María</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Beligni, Verónica</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lamattina, Lorenzo</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Physiologia plantarum</subfield><subfield code="d">Oxford [u.a.] : Wiley-Blackwell, 1948</subfield><subfield code="g">105(1999), 2, Seite 0</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ243927738</subfield><subfield code="w">(DE-600)2020837-6</subfield><subfield code="x">1399-3054</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:105</subfield><subfield code="g">year:1999</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:0</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">http://dx.doi.org/10.1034/j.1399-3054.1999.105216.x</subfield><subfield code="q">text/html</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DJB</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">105</subfield><subfield code="j">1999</subfield><subfield code="e">2</subfield><subfield code="h">0</subfield></datafield></record></collection>
|
| score |
7.3994074 |