Carbohydrate utilisation by cell suspension cultures of Phaseolus vulgaris
Uptake of sugar by Phaseolus vulgaris cell suspension cultures from a sucrose supplemented medium is predominantly in the hexose form. This is due to a rapid cleavage of the sucrose by an apoplastic acid invertase activity and an apparent very low demand for and uptake of carbon from the medium prio...
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| Autor*in: |
Botha, Frederik C. [verfasserIn] O'Kennedy, Maretha M. [verfasserIn] |
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| Format: |
E-Artikel |
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| Erschienen: |
Copenhagen: Munksgaard International Publishers ; 1998 |
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| Schlagwörter: |
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| Umfang: |
Online-Ressource |
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| Reproduktion: |
2008 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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| Übergeordnetes Werk: |
In: Physiologia plantarum - Oxford [u.a.] : Wiley-Blackwell, 1948, 102(1998), 3, Seite 0 |
| Übergeordnetes Werk: |
volume:102 ; year:1998 ; number:3 ; pages:0 |
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| DOI / URN: |
10.1034/j.1399-3054.1998.1020311.x |
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| 520 | |a Uptake of sugar by Phaseolus vulgaris cell suspension cultures from a sucrose supplemented medium is predominantly in the hexose form. This is due to a rapid cleavage of the sucrose by an apoplastic acid invertase activity and an apparent very low demand for and uptake of carbon from the medium prior to induction of cell growth and division. Glucose is preferentially taken up, leading to an accumulation of fructose in the medium. However, when the glucose is depleted the cells do take up the fructose at a rate similar to that of glucose. When glucose or fructose is supplied individually to cell cultures, both are utilised very efficiently with growth slightly better on the fructose medium. Hexose uptake is largely an active process with diffusion uptake even at the highest concentrations (> 50 mM) contributing less than 30%. The hexose uptake system of the cells has a greater affinity for glucose (Km= 240 µM) than for fructose (Km= 960 µM) but the maximum uptake (Vmax) is similar. The major difference in the kinetic properties of hexose uptake is that glucose is a strong inhibitor of fructose uptake, while fructose has little effect on glucose uptake. The differences in the kinetic properties of the uptake system for the two hexoses can largely explain the observed pattern of hexose utilisation when both glucose and fructose are present in the medium. | ||
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10.1034/j.1399-3054.1998.1020311.x doi (DE-627)NLEJ243791046 DE-627 ger DE-627 rakwb Botha, Frederik C. verfasserin aut Carbohydrate utilisation by cell suspension cultures of Phaseolus vulgaris Copenhagen Munksgaard International Publishers 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Uptake of sugar by Phaseolus vulgaris cell suspension cultures from a sucrose supplemented medium is predominantly in the hexose form. This is due to a rapid cleavage of the sucrose by an apoplastic acid invertase activity and an apparent very low demand for and uptake of carbon from the medium prior to induction of cell growth and division. Glucose is preferentially taken up, leading to an accumulation of fructose in the medium. However, when the glucose is depleted the cells do take up the fructose at a rate similar to that of glucose. When glucose or fructose is supplied individually to cell cultures, both are utilised very efficiently with growth slightly better on the fructose medium. Hexose uptake is largely an active process with diffusion uptake even at the highest concentrations (> 50 mM) contributing less than 30%. The hexose uptake system of the cells has a greater affinity for glucose (Km= 240 µM) than for fructose (Km= 960 µM) but the maximum uptake (Vmax) is similar. The major difference in the kinetic properties of hexose uptake is that glucose is a strong inhibitor of fructose uptake, while fructose has little effect on glucose uptake. The differences in the kinetic properties of the uptake system for the two hexoses can largely explain the observed pattern of hexose utilisation when both glucose and fructose are present in the medium. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Cell suspensions O'Kennedy, Maretha M. verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 102(1998), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:102 year:1998 number:3 pages:0 http://dx.doi.org/10.1034/j.1399-3054.1998.1020311.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 102 1998 3 0 |
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10.1034/j.1399-3054.1998.1020311.x doi (DE-627)NLEJ243791046 DE-627 ger DE-627 rakwb Botha, Frederik C. verfasserin aut Carbohydrate utilisation by cell suspension cultures of Phaseolus vulgaris Copenhagen Munksgaard International Publishers 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Uptake of sugar by Phaseolus vulgaris cell suspension cultures from a sucrose supplemented medium is predominantly in the hexose form. This is due to a rapid cleavage of the sucrose by an apoplastic acid invertase activity and an apparent very low demand for and uptake of carbon from the medium prior to induction of cell growth and division. Glucose is preferentially taken up, leading to an accumulation of fructose in the medium. However, when the glucose is depleted the cells do take up the fructose at a rate similar to that of glucose. When glucose or fructose is supplied individually to cell cultures, both are utilised very efficiently with growth slightly better on the fructose medium. Hexose uptake is largely an active process with diffusion uptake even at the highest concentrations (> 50 mM) contributing less than 30%. The hexose uptake system of the cells has a greater affinity for glucose (Km= 240 µM) than for fructose (Km= 960 µM) but the maximum uptake (Vmax) is similar. The major difference in the kinetic properties of hexose uptake is that glucose is a strong inhibitor of fructose uptake, while fructose has little effect on glucose uptake. The differences in the kinetic properties of the uptake system for the two hexoses can largely explain the observed pattern of hexose utilisation when both glucose and fructose are present in the medium. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Cell suspensions O'Kennedy, Maretha M. verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 102(1998), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:102 year:1998 number:3 pages:0 http://dx.doi.org/10.1034/j.1399-3054.1998.1020311.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 102 1998 3 0 |
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10.1034/j.1399-3054.1998.1020311.x doi (DE-627)NLEJ243791046 DE-627 ger DE-627 rakwb Botha, Frederik C. verfasserin aut Carbohydrate utilisation by cell suspension cultures of Phaseolus vulgaris Copenhagen Munksgaard International Publishers 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Uptake of sugar by Phaseolus vulgaris cell suspension cultures from a sucrose supplemented medium is predominantly in the hexose form. This is due to a rapid cleavage of the sucrose by an apoplastic acid invertase activity and an apparent very low demand for and uptake of carbon from the medium prior to induction of cell growth and division. Glucose is preferentially taken up, leading to an accumulation of fructose in the medium. However, when the glucose is depleted the cells do take up the fructose at a rate similar to that of glucose. When glucose or fructose is supplied individually to cell cultures, both are utilised very efficiently with growth slightly better on the fructose medium. Hexose uptake is largely an active process with diffusion uptake even at the highest concentrations (> 50 mM) contributing less than 30%. The hexose uptake system of the cells has a greater affinity for glucose (Km= 240 µM) than for fructose (Km= 960 µM) but the maximum uptake (Vmax) is similar. The major difference in the kinetic properties of hexose uptake is that glucose is a strong inhibitor of fructose uptake, while fructose has little effect on glucose uptake. The differences in the kinetic properties of the uptake system for the two hexoses can largely explain the observed pattern of hexose utilisation when both glucose and fructose are present in the medium. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Cell suspensions O'Kennedy, Maretha M. verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 102(1998), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:102 year:1998 number:3 pages:0 http://dx.doi.org/10.1034/j.1399-3054.1998.1020311.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 102 1998 3 0 |
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10.1034/j.1399-3054.1998.1020311.x doi (DE-627)NLEJ243791046 DE-627 ger DE-627 rakwb Botha, Frederik C. verfasserin aut Carbohydrate utilisation by cell suspension cultures of Phaseolus vulgaris Copenhagen Munksgaard International Publishers 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Uptake of sugar by Phaseolus vulgaris cell suspension cultures from a sucrose supplemented medium is predominantly in the hexose form. This is due to a rapid cleavage of the sucrose by an apoplastic acid invertase activity and an apparent very low demand for and uptake of carbon from the medium prior to induction of cell growth and division. Glucose is preferentially taken up, leading to an accumulation of fructose in the medium. However, when the glucose is depleted the cells do take up the fructose at a rate similar to that of glucose. When glucose or fructose is supplied individually to cell cultures, both are utilised very efficiently with growth slightly better on the fructose medium. Hexose uptake is largely an active process with diffusion uptake even at the highest concentrations (> 50 mM) contributing less than 30%. The hexose uptake system of the cells has a greater affinity for glucose (Km= 240 µM) than for fructose (Km= 960 µM) but the maximum uptake (Vmax) is similar. The major difference in the kinetic properties of hexose uptake is that glucose is a strong inhibitor of fructose uptake, while fructose has little effect on glucose uptake. The differences in the kinetic properties of the uptake system for the two hexoses can largely explain the observed pattern of hexose utilisation when both glucose and fructose are present in the medium. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Cell suspensions O'Kennedy, Maretha M. verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 102(1998), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:102 year:1998 number:3 pages:0 http://dx.doi.org/10.1034/j.1399-3054.1998.1020311.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 102 1998 3 0 |
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10.1034/j.1399-3054.1998.1020311.x doi (DE-627)NLEJ243791046 DE-627 ger DE-627 rakwb Botha, Frederik C. verfasserin aut Carbohydrate utilisation by cell suspension cultures of Phaseolus vulgaris Copenhagen Munksgaard International Publishers 1998 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Uptake of sugar by Phaseolus vulgaris cell suspension cultures from a sucrose supplemented medium is predominantly in the hexose form. This is due to a rapid cleavage of the sucrose by an apoplastic acid invertase activity and an apparent very low demand for and uptake of carbon from the medium prior to induction of cell growth and division. Glucose is preferentially taken up, leading to an accumulation of fructose in the medium. However, when the glucose is depleted the cells do take up the fructose at a rate similar to that of glucose. When glucose or fructose is supplied individually to cell cultures, both are utilised very efficiently with growth slightly better on the fructose medium. Hexose uptake is largely an active process with diffusion uptake even at the highest concentrations (> 50 mM) contributing less than 30%. The hexose uptake system of the cells has a greater affinity for glucose (Km= 240 µM) than for fructose (Km= 960 µM) but the maximum uptake (Vmax) is similar. The major difference in the kinetic properties of hexose uptake is that glucose is a strong inhibitor of fructose uptake, while fructose has little effect on glucose uptake. The differences in the kinetic properties of the uptake system for the two hexoses can largely explain the observed pattern of hexose utilisation when both glucose and fructose are present in the medium. 2008 Blackwell Publishing Journal Backfiles 1879-2005 |2008|||||||||| Cell suspensions O'Kennedy, Maretha M. verfasserin aut In Physiologia plantarum Oxford [u.a.] : Wiley-Blackwell, 1948 102(1998), 3, Seite 0 Online-Ressource (DE-627)NLEJ243927738 (DE-600)2020837-6 1399-3054 nnns volume:102 year:1998 number:3 pages:0 http://dx.doi.org/10.1034/j.1399-3054.1998.1020311.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 102 1998 3 0 |
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Carbohydrate utilisation by cell suspension cultures of Phaseolus vulgaris |
| abstract |
Uptake of sugar by Phaseolus vulgaris cell suspension cultures from a sucrose supplemented medium is predominantly in the hexose form. This is due to a rapid cleavage of the sucrose by an apoplastic acid invertase activity and an apparent very low demand for and uptake of carbon from the medium prior to induction of cell growth and division. Glucose is preferentially taken up, leading to an accumulation of fructose in the medium. However, when the glucose is depleted the cells do take up the fructose at a rate similar to that of glucose. When glucose or fructose is supplied individually to cell cultures, both are utilised very efficiently with growth slightly better on the fructose medium. Hexose uptake is largely an active process with diffusion uptake even at the highest concentrations (> 50 mM) contributing less than 30%. The hexose uptake system of the cells has a greater affinity for glucose (Km= 240 µM) than for fructose (Km= 960 µM) but the maximum uptake (Vmax) is similar. The major difference in the kinetic properties of hexose uptake is that glucose is a strong inhibitor of fructose uptake, while fructose has little effect on glucose uptake. The differences in the kinetic properties of the uptake system for the two hexoses can largely explain the observed pattern of hexose utilisation when both glucose and fructose are present in the medium. |
| abstractGer |
Uptake of sugar by Phaseolus vulgaris cell suspension cultures from a sucrose supplemented medium is predominantly in the hexose form. This is due to a rapid cleavage of the sucrose by an apoplastic acid invertase activity and an apparent very low demand for and uptake of carbon from the medium prior to induction of cell growth and division. Glucose is preferentially taken up, leading to an accumulation of fructose in the medium. However, when the glucose is depleted the cells do take up the fructose at a rate similar to that of glucose. When glucose or fructose is supplied individually to cell cultures, both are utilised very efficiently with growth slightly better on the fructose medium. Hexose uptake is largely an active process with diffusion uptake even at the highest concentrations (> 50 mM) contributing less than 30%. The hexose uptake system of the cells has a greater affinity for glucose (Km= 240 µM) than for fructose (Km= 960 µM) but the maximum uptake (Vmax) is similar. The major difference in the kinetic properties of hexose uptake is that glucose is a strong inhibitor of fructose uptake, while fructose has little effect on glucose uptake. The differences in the kinetic properties of the uptake system for the two hexoses can largely explain the observed pattern of hexose utilisation when both glucose and fructose are present in the medium. |
| abstract_unstemmed |
Uptake of sugar by Phaseolus vulgaris cell suspension cultures from a sucrose supplemented medium is predominantly in the hexose form. This is due to a rapid cleavage of the sucrose by an apoplastic acid invertase activity and an apparent very low demand for and uptake of carbon from the medium prior to induction of cell growth and division. Glucose is preferentially taken up, leading to an accumulation of fructose in the medium. However, when the glucose is depleted the cells do take up the fructose at a rate similar to that of glucose. When glucose or fructose is supplied individually to cell cultures, both are utilised very efficiently with growth slightly better on the fructose medium. Hexose uptake is largely an active process with diffusion uptake even at the highest concentrations (> 50 mM) contributing less than 30%. The hexose uptake system of the cells has a greater affinity for glucose (Km= 240 µM) than for fructose (Km= 960 µM) but the maximum uptake (Vmax) is similar. The major difference in the kinetic properties of hexose uptake is that glucose is a strong inhibitor of fructose uptake, while fructose has little effect on glucose uptake. The differences in the kinetic properties of the uptake system for the two hexoses can largely explain the observed pattern of hexose utilisation when both glucose and fructose are present in the medium. |
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O'Kennedy, Maretha M. |
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NLEJ243927738 |
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z |
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| doi_str |
10.1034/j.1399-3054.1998.1020311.x |
| up_date |
2024-07-06T06:33:12.487Z |
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1803810339541745664 |
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7.3979445 |