Linkage of Vfa4 in Malus × domestica and Malus floribunda with Vf resistance to the apple scab pathogen Venturia inaequalis
The Vf locus from Malus floribunda clone 821 is an important source of resistance to apple scab disease caused by Venturia inaequalis, and has been introduced into numerous cultivars of domesticated apple, Malus × domestica. Cloning of the putative Vf locus has revealed that it contains several rece...
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| Autor*in: |
Afunian, M. R. [verfasserIn] Goodwin, P. H. [verfasserIn] Hunter, D. M. [verfasserIn] |
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E-Artikel |
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| Erschienen: |
Oxford, UK: Blackwell Science Ltd ; 2004 |
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Online-Ressource |
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2004 ; Blackwell Publishing Journal Backfiles 1879-2005 |
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In: Plant pathology - Oxford [u.a.] : Wiley-Blackwell, 1952, 53(2004), 4, Seite 0 |
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volume:53 ; year:2004 ; number:4 ; pages:0 |
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10.1111/j.1365-3059.2004.01047.x |
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| 520 | |a The Vf locus from Malus floribunda clone 821 is an important source of resistance to apple scab disease caused by Venturia inaequalis, and has been introduced into numerous cultivars of domesticated apple, Malus × domestica. Cloning of the putative Vf locus has revealed that it contains several receptor-like Vf candidate genes. In order to determine which of these genes is most closely linked to Vf resistance, primers were designed based on conserved regions in the Vf candidate genes adjacent to a variable portion of the leucine-rich repeat (LRR) domain, to yield PCR product length polymorphisms. PCR products were obtained from 31 cultivars of M. × domestica, of which 19 are known to contain Vf resistance, and from 10 selections of M. floribunda. PCR products corresponding in size to Vfa1 and Vfa2 were found in all the plants tested. However, a PCR product with 100% predicted amino acid identity to Vfa4 was found only in M. ×domestica cultivars known to have Vf scab resistance. This PCR product was also found in most, but not all, selections of M. floribunda tested, including the original source of Vf resistance, M. floribunda 821. The PCR product matching Vfa4 appears to be the most closely linked to Vf resistance and should be a valuable tool for monitoring Vf inheritance in apple. | ||
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10.1111/j.1365-3059.2004.01047.x doi (DE-627)NLEJ24379620X DE-627 ger DE-627 rakwb Afunian, M. R. verfasserin aut Linkage of Vfa4 in Malus × domestica and Malus floribunda with Vf resistance to the apple scab pathogen Venturia inaequalis Oxford, UK Blackwell Science Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The Vf locus from Malus floribunda clone 821 is an important source of resistance to apple scab disease caused by Venturia inaequalis, and has been introduced into numerous cultivars of domesticated apple, Malus × domestica. Cloning of the putative Vf locus has revealed that it contains several receptor-like Vf candidate genes. In order to determine which of these genes is most closely linked to Vf resistance, primers were designed based on conserved regions in the Vf candidate genes adjacent to a variable portion of the leucine-rich repeat (LRR) domain, to yield PCR product length polymorphisms. PCR products were obtained from 31 cultivars of M. × domestica, of which 19 are known to contain Vf resistance, and from 10 selections of M. floribunda. PCR products corresponding in size to Vfa1 and Vfa2 were found in all the plants tested. However, a PCR product with 100% predicted amino acid identity to Vfa4 was found only in M. ×domestica cultivars known to have Vf scab resistance. This PCR product was also found in most, but not all, selections of M. floribunda tested, including the original source of Vf resistance, M. floribunda 821. The PCR product matching Vfa4 appears to be the most closely linked to Vf resistance and should be a valuable tool for monitoring Vf inheritance in apple. 2004 Blackwell Publishing Journal Backfiles 1879-2005 |2004|||||||||| apple scab Goodwin, P. H. verfasserin aut Hunter, D. M. verfasserin aut In Plant pathology Oxford [u.a.] : Wiley-Blackwell, 1952 53(2004), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927657 (DE-600)2020845-5 1365-3059 nnns volume:53 year:2004 number:4 pages:0 http://dx.doi.org/10.1111/j.1365-3059.2004.01047.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 53 2004 4 0 |
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10.1111/j.1365-3059.2004.01047.x doi (DE-627)NLEJ24379620X DE-627 ger DE-627 rakwb Afunian, M. R. verfasserin aut Linkage of Vfa4 in Malus × domestica and Malus floribunda with Vf resistance to the apple scab pathogen Venturia inaequalis Oxford, UK Blackwell Science Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The Vf locus from Malus floribunda clone 821 is an important source of resistance to apple scab disease caused by Venturia inaequalis, and has been introduced into numerous cultivars of domesticated apple, Malus × domestica. Cloning of the putative Vf locus has revealed that it contains several receptor-like Vf candidate genes. In order to determine which of these genes is most closely linked to Vf resistance, primers were designed based on conserved regions in the Vf candidate genes adjacent to a variable portion of the leucine-rich repeat (LRR) domain, to yield PCR product length polymorphisms. PCR products were obtained from 31 cultivars of M. × domestica, of which 19 are known to contain Vf resistance, and from 10 selections of M. floribunda. PCR products corresponding in size to Vfa1 and Vfa2 were found in all the plants tested. However, a PCR product with 100% predicted amino acid identity to Vfa4 was found only in M. ×domestica cultivars known to have Vf scab resistance. This PCR product was also found in most, but not all, selections of M. floribunda tested, including the original source of Vf resistance, M. floribunda 821. The PCR product matching Vfa4 appears to be the most closely linked to Vf resistance and should be a valuable tool for monitoring Vf inheritance in apple. 2004 Blackwell Publishing Journal Backfiles 1879-2005 |2004|||||||||| apple scab Goodwin, P. H. verfasserin aut Hunter, D. M. verfasserin aut In Plant pathology Oxford [u.a.] : Wiley-Blackwell, 1952 53(2004), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927657 (DE-600)2020845-5 1365-3059 nnns volume:53 year:2004 number:4 pages:0 http://dx.doi.org/10.1111/j.1365-3059.2004.01047.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 53 2004 4 0 |
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10.1111/j.1365-3059.2004.01047.x doi (DE-627)NLEJ24379620X DE-627 ger DE-627 rakwb Afunian, M. R. verfasserin aut Linkage of Vfa4 in Malus × domestica and Malus floribunda with Vf resistance to the apple scab pathogen Venturia inaequalis Oxford, UK Blackwell Science Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The Vf locus from Malus floribunda clone 821 is an important source of resistance to apple scab disease caused by Venturia inaequalis, and has been introduced into numerous cultivars of domesticated apple, Malus × domestica. Cloning of the putative Vf locus has revealed that it contains several receptor-like Vf candidate genes. In order to determine which of these genes is most closely linked to Vf resistance, primers were designed based on conserved regions in the Vf candidate genes adjacent to a variable portion of the leucine-rich repeat (LRR) domain, to yield PCR product length polymorphisms. PCR products were obtained from 31 cultivars of M. × domestica, of which 19 are known to contain Vf resistance, and from 10 selections of M. floribunda. PCR products corresponding in size to Vfa1 and Vfa2 were found in all the plants tested. However, a PCR product with 100% predicted amino acid identity to Vfa4 was found only in M. ×domestica cultivars known to have Vf scab resistance. This PCR product was also found in most, but not all, selections of M. floribunda tested, including the original source of Vf resistance, M. floribunda 821. The PCR product matching Vfa4 appears to be the most closely linked to Vf resistance and should be a valuable tool for monitoring Vf inheritance in apple. 2004 Blackwell Publishing Journal Backfiles 1879-2005 |2004|||||||||| apple scab Goodwin, P. H. verfasserin aut Hunter, D. M. verfasserin aut In Plant pathology Oxford [u.a.] : Wiley-Blackwell, 1952 53(2004), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927657 (DE-600)2020845-5 1365-3059 nnns volume:53 year:2004 number:4 pages:0 http://dx.doi.org/10.1111/j.1365-3059.2004.01047.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 53 2004 4 0 |
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10.1111/j.1365-3059.2004.01047.x doi (DE-627)NLEJ24379620X DE-627 ger DE-627 rakwb Afunian, M. R. verfasserin aut Linkage of Vfa4 in Malus × domestica and Malus floribunda with Vf resistance to the apple scab pathogen Venturia inaequalis Oxford, UK Blackwell Science Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The Vf locus from Malus floribunda clone 821 is an important source of resistance to apple scab disease caused by Venturia inaequalis, and has been introduced into numerous cultivars of domesticated apple, Malus × domestica. Cloning of the putative Vf locus has revealed that it contains several receptor-like Vf candidate genes. In order to determine which of these genes is most closely linked to Vf resistance, primers were designed based on conserved regions in the Vf candidate genes adjacent to a variable portion of the leucine-rich repeat (LRR) domain, to yield PCR product length polymorphisms. PCR products were obtained from 31 cultivars of M. × domestica, of which 19 are known to contain Vf resistance, and from 10 selections of M. floribunda. PCR products corresponding in size to Vfa1 and Vfa2 were found in all the plants tested. However, a PCR product with 100% predicted amino acid identity to Vfa4 was found only in M. ×domestica cultivars known to have Vf scab resistance. This PCR product was also found in most, but not all, selections of M. floribunda tested, including the original source of Vf resistance, M. floribunda 821. The PCR product matching Vfa4 appears to be the most closely linked to Vf resistance and should be a valuable tool for monitoring Vf inheritance in apple. 2004 Blackwell Publishing Journal Backfiles 1879-2005 |2004|||||||||| apple scab Goodwin, P. H. verfasserin aut Hunter, D. M. verfasserin aut In Plant pathology Oxford [u.a.] : Wiley-Blackwell, 1952 53(2004), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927657 (DE-600)2020845-5 1365-3059 nnns volume:53 year:2004 number:4 pages:0 http://dx.doi.org/10.1111/j.1365-3059.2004.01047.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 53 2004 4 0 |
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10.1111/j.1365-3059.2004.01047.x doi (DE-627)NLEJ24379620X DE-627 ger DE-627 rakwb Afunian, M. R. verfasserin aut Linkage of Vfa4 in Malus × domestica and Malus floribunda with Vf resistance to the apple scab pathogen Venturia inaequalis Oxford, UK Blackwell Science Ltd 2004 Online-Ressource nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The Vf locus from Malus floribunda clone 821 is an important source of resistance to apple scab disease caused by Venturia inaequalis, and has been introduced into numerous cultivars of domesticated apple, Malus × domestica. Cloning of the putative Vf locus has revealed that it contains several receptor-like Vf candidate genes. In order to determine which of these genes is most closely linked to Vf resistance, primers were designed based on conserved regions in the Vf candidate genes adjacent to a variable portion of the leucine-rich repeat (LRR) domain, to yield PCR product length polymorphisms. PCR products were obtained from 31 cultivars of M. × domestica, of which 19 are known to contain Vf resistance, and from 10 selections of M. floribunda. PCR products corresponding in size to Vfa1 and Vfa2 were found in all the plants tested. However, a PCR product with 100% predicted amino acid identity to Vfa4 was found only in M. ×domestica cultivars known to have Vf scab resistance. This PCR product was also found in most, but not all, selections of M. floribunda tested, including the original source of Vf resistance, M. floribunda 821. The PCR product matching Vfa4 appears to be the most closely linked to Vf resistance and should be a valuable tool for monitoring Vf inheritance in apple. 2004 Blackwell Publishing Journal Backfiles 1879-2005 |2004|||||||||| apple scab Goodwin, P. H. verfasserin aut Hunter, D. M. verfasserin aut In Plant pathology Oxford [u.a.] : Wiley-Blackwell, 1952 53(2004), 4, Seite 0 Online-Ressource (DE-627)NLEJ243927657 (DE-600)2020845-5 1365-3059 nnns volume:53 year:2004 number:4 pages:0 http://dx.doi.org/10.1111/j.1365-3059.2004.01047.x text/html Verlag Deutschlandweit zugänglich Volltext GBV_USEFLAG_U ZDB-1-DJB GBV_NL_ARTICLE AR 53 2004 4 0 |
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Linkage of Vfa4 in Malus × domestica and Malus floribunda with Vf resistance to the apple scab pathogen Venturia inaequalis apple scab |
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Linkage of Vfa4 in Malus × domestica and Malus floribunda with Vf resistance to the apple scab pathogen Venturia inaequalis |
| abstract |
The Vf locus from Malus floribunda clone 821 is an important source of resistance to apple scab disease caused by Venturia inaequalis, and has been introduced into numerous cultivars of domesticated apple, Malus × domestica. Cloning of the putative Vf locus has revealed that it contains several receptor-like Vf candidate genes. In order to determine which of these genes is most closely linked to Vf resistance, primers were designed based on conserved regions in the Vf candidate genes adjacent to a variable portion of the leucine-rich repeat (LRR) domain, to yield PCR product length polymorphisms. PCR products were obtained from 31 cultivars of M. × domestica, of which 19 are known to contain Vf resistance, and from 10 selections of M. floribunda. PCR products corresponding in size to Vfa1 and Vfa2 were found in all the plants tested. However, a PCR product with 100% predicted amino acid identity to Vfa4 was found only in M. ×domestica cultivars known to have Vf scab resistance. This PCR product was also found in most, but not all, selections of M. floribunda tested, including the original source of Vf resistance, M. floribunda 821. The PCR product matching Vfa4 appears to be the most closely linked to Vf resistance and should be a valuable tool for monitoring Vf inheritance in apple. |
| abstractGer |
The Vf locus from Malus floribunda clone 821 is an important source of resistance to apple scab disease caused by Venturia inaequalis, and has been introduced into numerous cultivars of domesticated apple, Malus × domestica. Cloning of the putative Vf locus has revealed that it contains several receptor-like Vf candidate genes. In order to determine which of these genes is most closely linked to Vf resistance, primers were designed based on conserved regions in the Vf candidate genes adjacent to a variable portion of the leucine-rich repeat (LRR) domain, to yield PCR product length polymorphisms. PCR products were obtained from 31 cultivars of M. × domestica, of which 19 are known to contain Vf resistance, and from 10 selections of M. floribunda. PCR products corresponding in size to Vfa1 and Vfa2 were found in all the plants tested. However, a PCR product with 100% predicted amino acid identity to Vfa4 was found only in M. ×domestica cultivars known to have Vf scab resistance. This PCR product was also found in most, but not all, selections of M. floribunda tested, including the original source of Vf resistance, M. floribunda 821. The PCR product matching Vfa4 appears to be the most closely linked to Vf resistance and should be a valuable tool for monitoring Vf inheritance in apple. |
| abstract_unstemmed |
The Vf locus from Malus floribunda clone 821 is an important source of resistance to apple scab disease caused by Venturia inaequalis, and has been introduced into numerous cultivars of domesticated apple, Malus × domestica. Cloning of the putative Vf locus has revealed that it contains several receptor-like Vf candidate genes. In order to determine which of these genes is most closely linked to Vf resistance, primers were designed based on conserved regions in the Vf candidate genes adjacent to a variable portion of the leucine-rich repeat (LRR) domain, to yield PCR product length polymorphisms. PCR products were obtained from 31 cultivars of M. × domestica, of which 19 are known to contain Vf resistance, and from 10 selections of M. floribunda. PCR products corresponding in size to Vfa1 and Vfa2 were found in all the plants tested. However, a PCR product with 100% predicted amino acid identity to Vfa4 was found only in M. ×domestica cultivars known to have Vf scab resistance. This PCR product was also found in most, but not all, selections of M. floribunda tested, including the original source of Vf resistance, M. floribunda 821. The PCR product matching Vfa4 appears to be the most closely linked to Vf resistance and should be a valuable tool for monitoring Vf inheritance in apple. |
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4 |
| title_short |
Linkage of Vfa4 in Malus × domestica and Malus floribunda with Vf resistance to the apple scab pathogen Venturia inaequalis |
| url |
http://dx.doi.org/10.1111/j.1365-3059.2004.01047.x |
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| author2 |
Goodwin, P. H. Hunter, D. M. |
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Goodwin, P. H. Hunter, D. M. |
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| doi_str |
10.1111/j.1365-3059.2004.01047.x |
| up_date |
2024-07-06T06:34:13.729Z |
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