Testis-Specific Expression of the Nuclear Form of Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx)
The selenoprotein phospholipid hydroperoxide glutathioneperoxidase (PHGPx) is present in at leastthree different isoforms in testis: as a cytosolic, as amitochondrial, and as a nuclear protein. We have recentlyshown that a sperm nucleus-specific glutathioneperoxidase (snGPx) is identical to the mito...
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Copyright © 2003 by Walter de Gruyter GmbH & Co. KG |
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Walter de Gruyter Online Zeitschriften |
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In: 384(2005), 4 vom: 01. Juni, Seite 635-643 volume:384 ; year:2005 ; number:4 ; day:01 ; month:06 ; pages:635-643 ; extent:9 |
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10.1515/BC.2003.070 |
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| 520 | |a The selenoprotein phospholipid hydroperoxide glutathioneperoxidase (PHGPx) is present in at leastthree different isoforms in testis: as a cytosolic, as amitochondrial, and as a nuclear protein. We have recentlyshown that a sperm nucleus-specific glutathioneperoxidase (snGPx) is identical to the mitochondrialand cytosolic forms of PHGPx apart fromits N-terminus. This arginine-rich N-terminus of snGPx, reminiscent of protamines, is encoded by an alternativeexon located in the first intron of the PHGPx gene and is responsible for nuclear localisation and chromatin binding of snGPx [Pfeifer et al., FASEB J.15 (2001), pp. 1236-1238]. By using a combination oftechniques including selective cloning of mRNA 5'ends, RT-PCR, and S1 analyses, we provide evidencethat the transcript encoding the nuclear form is generatedby transcription initiation at an alternative promoterand not by alternative splicing. We show thatthe major transcription start region is located at 12to 14 upstream of the AUG translation initiation siteof the sperm nucleus-specific exon and lacks a TATAbox. Two minor TATA-less transcription initiation sitesare located at around -30 and -45. We have shown byin situ hybridisation that snGPx expression in testis,like protamine expression, is restricted to late stagesof spermatogenesis whereas PHGPx expression isonly found in spermatocytes and early spermatids.These findings have to be taken into account whenstudying either the differential regulation of PHGPxand snGPx expression in testis or the impact of putativemutations in snGPx on male fertility in man. | ||
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| 700 | 1 | |a Conrad, M. |4 oth | |
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10.1515/BC.2003.070 doi artikel_Grundlieferung.pp (DE-627)NLEJ246516143 DE-627 ger DE-627 rakwb Testis-Specific Expression of the Nuclear Form of Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx) Walter de Gruyter 2005 9 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Copyright © 2003 by Walter de Gruyter GmbH & Co. KG The selenoprotein phospholipid hydroperoxide glutathioneperoxidase (PHGPx) is present in at leastthree different isoforms in testis: as a cytosolic, as amitochondrial, and as a nuclear protein. We have recentlyshown that a sperm nucleus-specific glutathioneperoxidase (snGPx) is identical to the mitochondrialand cytosolic forms of PHGPx apart fromits N-terminus. This arginine-rich N-terminus of snGPx, reminiscent of protamines, is encoded by an alternativeexon located in the first intron of the PHGPx gene and is responsible for nuclear localisation and chromatin binding of snGPx [Pfeifer et al., FASEB J.15 (2001), pp. 1236-1238]. By using a combination oftechniques including selective cloning of mRNA 5'ends, RT-PCR, and S1 analyses, we provide evidencethat the transcript encoding the nuclear form is generatedby transcription initiation at an alternative promoterand not by alternative splicing. We show thatthe major transcription start region is located at 12to 14 upstream of the AUG translation initiation siteof the sperm nucleus-specific exon and lacks a TATAbox. Two minor TATA-less transcription initiation sitesare located at around -30 and -45. We have shown byin situ hybridisation that snGPx expression in testis,like protamine expression, is restricted to late stagesof spermatogenesis whereas PHGPx expression isonly found in spermatocytes and early spermatids.These findings have to be taken into account whenstudying either the differential regulation of PHGPxand snGPx expression in testis or the impact of putativemutations in snGPx on male fertility in man. Walter de Gruyter Online Zeitschriften Moreno, S. G. oth Laux, G. oth Brielmeier, M. oth Bornkamm, G. W. oth Conrad, M. oth In 384(2005), 4 vom: 01. Juni, Seite 635-643 volume:384 year:2005 number:4 day:01 month:06 pages:635-643 extent:9 https://doi.org/10.1515/BC.2003.070 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 384 2005 4 01 06 635-643 9 |
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10.1515/BC.2003.070 doi artikel_Grundlieferung.pp (DE-627)NLEJ246516143 DE-627 ger DE-627 rakwb Testis-Specific Expression of the Nuclear Form of Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx) Walter de Gruyter 2005 9 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Copyright © 2003 by Walter de Gruyter GmbH & Co. KG The selenoprotein phospholipid hydroperoxide glutathioneperoxidase (PHGPx) is present in at leastthree different isoforms in testis: as a cytosolic, as amitochondrial, and as a nuclear protein. We have recentlyshown that a sperm nucleus-specific glutathioneperoxidase (snGPx) is identical to the mitochondrialand cytosolic forms of PHGPx apart fromits N-terminus. This arginine-rich N-terminus of snGPx, reminiscent of protamines, is encoded by an alternativeexon located in the first intron of the PHGPx gene and is responsible for nuclear localisation and chromatin binding of snGPx [Pfeifer et al., FASEB J.15 (2001), pp. 1236-1238]. By using a combination oftechniques including selective cloning of mRNA 5'ends, RT-PCR, and S1 analyses, we provide evidencethat the transcript encoding the nuclear form is generatedby transcription initiation at an alternative promoterand not by alternative splicing. We show thatthe major transcription start region is located at 12to 14 upstream of the AUG translation initiation siteof the sperm nucleus-specific exon and lacks a TATAbox. Two minor TATA-less transcription initiation sitesare located at around -30 and -45. We have shown byin situ hybridisation that snGPx expression in testis,like protamine expression, is restricted to late stagesof spermatogenesis whereas PHGPx expression isonly found in spermatocytes and early spermatids.These findings have to be taken into account whenstudying either the differential regulation of PHGPxand snGPx expression in testis or the impact of putativemutations in snGPx on male fertility in man. Walter de Gruyter Online Zeitschriften Moreno, S. G. oth Laux, G. oth Brielmeier, M. oth Bornkamm, G. W. oth Conrad, M. oth In 384(2005), 4 vom: 01. Juni, Seite 635-643 volume:384 year:2005 number:4 day:01 month:06 pages:635-643 extent:9 https://doi.org/10.1515/BC.2003.070 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 384 2005 4 01 06 635-643 9 |
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10.1515/BC.2003.070 doi artikel_Grundlieferung.pp (DE-627)NLEJ246516143 DE-627 ger DE-627 rakwb Testis-Specific Expression of the Nuclear Form of Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx) Walter de Gruyter 2005 9 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Copyright © 2003 by Walter de Gruyter GmbH & Co. KG The selenoprotein phospholipid hydroperoxide glutathioneperoxidase (PHGPx) is present in at leastthree different isoforms in testis: as a cytosolic, as amitochondrial, and as a nuclear protein. We have recentlyshown that a sperm nucleus-specific glutathioneperoxidase (snGPx) is identical to the mitochondrialand cytosolic forms of PHGPx apart fromits N-terminus. This arginine-rich N-terminus of snGPx, reminiscent of protamines, is encoded by an alternativeexon located in the first intron of the PHGPx gene and is responsible for nuclear localisation and chromatin binding of snGPx [Pfeifer et al., FASEB J.15 (2001), pp. 1236-1238]. By using a combination oftechniques including selective cloning of mRNA 5'ends, RT-PCR, and S1 analyses, we provide evidencethat the transcript encoding the nuclear form is generatedby transcription initiation at an alternative promoterand not by alternative splicing. We show thatthe major transcription start region is located at 12to 14 upstream of the AUG translation initiation siteof the sperm nucleus-specific exon and lacks a TATAbox. Two minor TATA-less transcription initiation sitesare located at around -30 and -45. We have shown byin situ hybridisation that snGPx expression in testis,like protamine expression, is restricted to late stagesof spermatogenesis whereas PHGPx expression isonly found in spermatocytes and early spermatids.These findings have to be taken into account whenstudying either the differential regulation of PHGPxand snGPx expression in testis or the impact of putativemutations in snGPx on male fertility in man. Walter de Gruyter Online Zeitschriften Moreno, S. G. oth Laux, G. oth Brielmeier, M. oth Bornkamm, G. W. oth Conrad, M. oth In 384(2005), 4 vom: 01. Juni, Seite 635-643 volume:384 year:2005 number:4 day:01 month:06 pages:635-643 extent:9 https://doi.org/10.1515/BC.2003.070 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 384 2005 4 01 06 635-643 9 |
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10.1515/BC.2003.070 doi artikel_Grundlieferung.pp (DE-627)NLEJ246516143 DE-627 ger DE-627 rakwb Testis-Specific Expression of the Nuclear Form of Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx) Walter de Gruyter 2005 9 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Copyright © 2003 by Walter de Gruyter GmbH & Co. KG The selenoprotein phospholipid hydroperoxide glutathioneperoxidase (PHGPx) is present in at leastthree different isoforms in testis: as a cytosolic, as amitochondrial, and as a nuclear protein. We have recentlyshown that a sperm nucleus-specific glutathioneperoxidase (snGPx) is identical to the mitochondrialand cytosolic forms of PHGPx apart fromits N-terminus. This arginine-rich N-terminus of snGPx, reminiscent of protamines, is encoded by an alternativeexon located in the first intron of the PHGPx gene and is responsible for nuclear localisation and chromatin binding of snGPx [Pfeifer et al., FASEB J.15 (2001), pp. 1236-1238]. By using a combination oftechniques including selective cloning of mRNA 5'ends, RT-PCR, and S1 analyses, we provide evidencethat the transcript encoding the nuclear form is generatedby transcription initiation at an alternative promoterand not by alternative splicing. We show thatthe major transcription start region is located at 12to 14 upstream of the AUG translation initiation siteof the sperm nucleus-specific exon and lacks a TATAbox. Two minor TATA-less transcription initiation sitesare located at around -30 and -45. We have shown byin situ hybridisation that snGPx expression in testis,like protamine expression, is restricted to late stagesof spermatogenesis whereas PHGPx expression isonly found in spermatocytes and early spermatids.These findings have to be taken into account whenstudying either the differential regulation of PHGPxand snGPx expression in testis or the impact of putativemutations in snGPx on male fertility in man. Walter de Gruyter Online Zeitschriften Moreno, S. G. oth Laux, G. oth Brielmeier, M. oth Bornkamm, G. W. oth Conrad, M. oth In 384(2005), 4 vom: 01. Juni, Seite 635-643 volume:384 year:2005 number:4 day:01 month:06 pages:635-643 extent:9 https://doi.org/10.1515/BC.2003.070 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 384 2005 4 01 06 635-643 9 |
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10.1515/BC.2003.070 doi artikel_Grundlieferung.pp (DE-627)NLEJ246516143 DE-627 ger DE-627 rakwb Testis-Specific Expression of the Nuclear Form of Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx) Walter de Gruyter 2005 9 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Copyright © 2003 by Walter de Gruyter GmbH & Co. KG The selenoprotein phospholipid hydroperoxide glutathioneperoxidase (PHGPx) is present in at leastthree different isoforms in testis: as a cytosolic, as amitochondrial, and as a nuclear protein. We have recentlyshown that a sperm nucleus-specific glutathioneperoxidase (snGPx) is identical to the mitochondrialand cytosolic forms of PHGPx apart fromits N-terminus. This arginine-rich N-terminus of snGPx, reminiscent of protamines, is encoded by an alternativeexon located in the first intron of the PHGPx gene and is responsible for nuclear localisation and chromatin binding of snGPx [Pfeifer et al., FASEB J.15 (2001), pp. 1236-1238]. By using a combination oftechniques including selective cloning of mRNA 5'ends, RT-PCR, and S1 analyses, we provide evidencethat the transcript encoding the nuclear form is generatedby transcription initiation at an alternative promoterand not by alternative splicing. We show thatthe major transcription start region is located at 12to 14 upstream of the AUG translation initiation siteof the sperm nucleus-specific exon and lacks a TATAbox. Two minor TATA-less transcription initiation sitesare located at around -30 and -45. We have shown byin situ hybridisation that snGPx expression in testis,like protamine expression, is restricted to late stagesof spermatogenesis whereas PHGPx expression isonly found in spermatocytes and early spermatids.These findings have to be taken into account whenstudying either the differential regulation of PHGPxand snGPx expression in testis or the impact of putativemutations in snGPx on male fertility in man. Walter de Gruyter Online Zeitschriften Moreno, S. G. oth Laux, G. oth Brielmeier, M. oth Bornkamm, G. W. oth Conrad, M. oth In 384(2005), 4 vom: 01. Juni, Seite 635-643 volume:384 year:2005 number:4 day:01 month:06 pages:635-643 extent:9 https://doi.org/10.1515/BC.2003.070 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 384 2005 4 01 06 635-643 9 |
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testis-specific expression of the nuclear form of phospholipid hydroperoxide glutathione peroxidase (phgpx) |
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Testis-Specific Expression of the Nuclear Form of Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx) |
| abstract |
The selenoprotein phospholipid hydroperoxide glutathioneperoxidase (PHGPx) is present in at leastthree different isoforms in testis: as a cytosolic, as amitochondrial, and as a nuclear protein. We have recentlyshown that a sperm nucleus-specific glutathioneperoxidase (snGPx) is identical to the mitochondrialand cytosolic forms of PHGPx apart fromits N-terminus. This arginine-rich N-terminus of snGPx, reminiscent of protamines, is encoded by an alternativeexon located in the first intron of the PHGPx gene and is responsible for nuclear localisation and chromatin binding of snGPx [Pfeifer et al., FASEB J.15 (2001), pp. 1236-1238]. By using a combination oftechniques including selective cloning of mRNA 5'ends, RT-PCR, and S1 analyses, we provide evidencethat the transcript encoding the nuclear form is generatedby transcription initiation at an alternative promoterand not by alternative splicing. We show thatthe major transcription start region is located at 12to 14 upstream of the AUG translation initiation siteof the sperm nucleus-specific exon and lacks a TATAbox. Two minor TATA-less transcription initiation sitesare located at around -30 and -45. We have shown byin situ hybridisation that snGPx expression in testis,like protamine expression, is restricted to late stagesof spermatogenesis whereas PHGPx expression isonly found in spermatocytes and early spermatids.These findings have to be taken into account whenstudying either the differential regulation of PHGPxand snGPx expression in testis or the impact of putativemutations in snGPx on male fertility in man. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG |
| abstractGer |
The selenoprotein phospholipid hydroperoxide glutathioneperoxidase (PHGPx) is present in at leastthree different isoforms in testis: as a cytosolic, as amitochondrial, and as a nuclear protein. We have recentlyshown that a sperm nucleus-specific glutathioneperoxidase (snGPx) is identical to the mitochondrialand cytosolic forms of PHGPx apart fromits N-terminus. This arginine-rich N-terminus of snGPx, reminiscent of protamines, is encoded by an alternativeexon located in the first intron of the PHGPx gene and is responsible for nuclear localisation and chromatin binding of snGPx [Pfeifer et al., FASEB J.15 (2001), pp. 1236-1238]. By using a combination oftechniques including selective cloning of mRNA 5'ends, RT-PCR, and S1 analyses, we provide evidencethat the transcript encoding the nuclear form is generatedby transcription initiation at an alternative promoterand not by alternative splicing. We show thatthe major transcription start region is located at 12to 14 upstream of the AUG translation initiation siteof the sperm nucleus-specific exon and lacks a TATAbox. Two minor TATA-less transcription initiation sitesare located at around -30 and -45. We have shown byin situ hybridisation that snGPx expression in testis,like protamine expression, is restricted to late stagesof spermatogenesis whereas PHGPx expression isonly found in spermatocytes and early spermatids.These findings have to be taken into account whenstudying either the differential regulation of PHGPxand snGPx expression in testis or the impact of putativemutations in snGPx on male fertility in man. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG |
| abstract_unstemmed |
The selenoprotein phospholipid hydroperoxide glutathioneperoxidase (PHGPx) is present in at leastthree different isoforms in testis: as a cytosolic, as amitochondrial, and as a nuclear protein. We have recentlyshown that a sperm nucleus-specific glutathioneperoxidase (snGPx) is identical to the mitochondrialand cytosolic forms of PHGPx apart fromits N-terminus. This arginine-rich N-terminus of snGPx, reminiscent of protamines, is encoded by an alternativeexon located in the first intron of the PHGPx gene and is responsible for nuclear localisation and chromatin binding of snGPx [Pfeifer et al., FASEB J.15 (2001), pp. 1236-1238]. By using a combination oftechniques including selective cloning of mRNA 5'ends, RT-PCR, and S1 analyses, we provide evidencethat the transcript encoding the nuclear form is generatedby transcription initiation at an alternative promoterand not by alternative splicing. We show thatthe major transcription start region is located at 12to 14 upstream of the AUG translation initiation siteof the sperm nucleus-specific exon and lacks a TATAbox. Two minor TATA-less transcription initiation sitesare located at around -30 and -45. We have shown byin situ hybridisation that snGPx expression in testis,like protamine expression, is restricted to late stagesof spermatogenesis whereas PHGPx expression isonly found in spermatocytes and early spermatids.These findings have to be taken into account whenstudying either the differential regulation of PHGPxand snGPx expression in testis or the impact of putativemutations in snGPx on male fertility in man. Copyright © 2003 by Walter de Gruyter GmbH & Co. KG |
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Testis-Specific Expression of the Nuclear Form of Phospholipid Hydroperoxide Glutathione Peroxidase (PHGPx) |
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Moreno, S. G. Laux, G. Brielmeier, M. Bornkamm, G. W. Conrad, M. |
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