Molecular and functional analysis of new members of the wheat PR4 gene family
Five new genes belonging to the pathogenesis-related (PR) 4 family have been cloned and characterised in Triticum aestivum. Two full-length genes, named wPR4e and wPR4f-b, were isolated by library screening, demonstrating the presence of a small intron only in wPR4f-b. Two other PR4 genes (wPR4f-a a...
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Walter de Gruyter ; 2006 |
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©2006 by Walter de Gruyter Berlin New York |
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Walter de Gruyter Online Zeitschriften |
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Enthalten in: Biological chemistry - Berlin [u.a.] : de Gruyter, 1996, 387(2006), 8 vom: 09. Aug., Seite 1101-1111 |
| Übergeordnetes Werk: |
volume:387 ; year:2006 ; number:8 ; day:09 ; month:08 ; pages:1101-1111 ; extent:11 |
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| DOI / URN: |
10.1515/BC.2006.136 |
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NLEJ24652166X |
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| 520 | |a Five new genes belonging to the pathogenesis-related (PR) 4 family have been cloned and characterised in Triticum aestivum. Two full-length genes, named wPR4e and wPR4f-b, were isolated by library screening, demonstrating the presence of a small intron only in wPR4f-b. Two other PR4 genes (wPR4f-a and wPR4f-c) were isolated by PCR, showing very high sequence identity with wPR4f-b and constituting a new sub-family. Transcription start analysis was performed by RLM-RACE, leading to the isolation of a fifth gene, named wPR4g, that is highly homologous to wPR4e; both encode putative vacuolar PR4 proteins (Wheatwin7 and Wheatwin5, respectively). wPR4e and wPR4f sub-family genes are induced by F. culmorum infection, by chemicals that lead to systemic acquired resistance and by wounding, showing different spatial and temporal induction pathways. In silico analysis of the 5′ untranslated regions of wPR4e and wPR4f-b revealed the presence of several abiotic and biotic stress-responsive elements. wPR4e and wPR4f-b putative promoters were fused to the β-glucuronidase (GUS) reporter gene, and transient and stable expression assays demonstrated that both are able to drive expression of GUS. Characterisation of these new PR4 genes and particularly of their 5′ untranslated regions, as well as the determination of their expression patterns, will contribute to our understanding of the responsiveness of this gene family to various stress conditions and of its role in plant defence. | ||
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10.1515/BC.2006.136 doi artikel_Grundlieferung.pp (DE-627)NLEJ24652166X DE-627 ger DE-627 rakwb Molecular and functional analysis of new members of the wheat PR4 gene family Walter de Gruyter 2006 11 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2006 by Walter de Gruyter Berlin New York Five new genes belonging to the pathogenesis-related (PR) 4 family have been cloned and characterised in Triticum aestivum. Two full-length genes, named wPR4e and wPR4f-b, were isolated by library screening, demonstrating the presence of a small intron only in wPR4f-b. Two other PR4 genes (wPR4f-a and wPR4f-c) were isolated by PCR, showing very high sequence identity with wPR4f-b and constituting a new sub-family. Transcription start analysis was performed by RLM-RACE, leading to the isolation of a fifth gene, named wPR4g, that is highly homologous to wPR4e; both encode putative vacuolar PR4 proteins (Wheatwin7 and Wheatwin5, respectively). wPR4e and wPR4f sub-family genes are induced by F. culmorum infection, by chemicals that lead to systemic acquired resistance and by wounding, showing different spatial and temporal induction pathways. In silico analysis of the 5′ untranslated regions of wPR4e and wPR4f-b revealed the presence of several abiotic and biotic stress-responsive elements. wPR4e and wPR4f-b putative promoters were fused to the β-glucuronidase (GUS) reporter gene, and transient and stable expression assays demonstrated that both are able to drive expression of GUS. Characterisation of these new PR4 genes and particularly of their 5′ untranslated regions, as well as the determination of their expression patterns, will contribute to our understanding of the responsiveness of this gene family to various stress conditions and of its role in plant defence. Walter de Gruyter Online Zeitschriften defence genes functional gene promoter gene expression PR4 genes promoter analysis SAR inducers Bertini, Laura oth Cascone, Annunziata oth Tucci, Marina oth D'Amore, Rosalinda oth Di Berardino, Iris oth Buonocore, Vincenzo oth Caporale, Carlo oth Caruso, Carla oth Enthalten in Biological chemistry Berlin [u.a.] : de Gruyter, 1996 387(2006), 8 vom: 09. Aug., Seite 1101-1111 (DE-627)NLEJ248235095 (DE-600)1466062-3 1437-4315 nnns volume:387 year:2006 number:8 day:09 month:08 pages:1101-1111 extent:11 https://doi.org/10.1515/BC.2006.136 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 387 2006 8 09 08 1101-1111 11 |
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10.1515/BC.2006.136 doi artikel_Grundlieferung.pp (DE-627)NLEJ24652166X DE-627 ger DE-627 rakwb Molecular and functional analysis of new members of the wheat PR4 gene family Walter de Gruyter 2006 11 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2006 by Walter de Gruyter Berlin New York Five new genes belonging to the pathogenesis-related (PR) 4 family have been cloned and characterised in Triticum aestivum. Two full-length genes, named wPR4e and wPR4f-b, were isolated by library screening, demonstrating the presence of a small intron only in wPR4f-b. Two other PR4 genes (wPR4f-a and wPR4f-c) were isolated by PCR, showing very high sequence identity with wPR4f-b and constituting a new sub-family. Transcription start analysis was performed by RLM-RACE, leading to the isolation of a fifth gene, named wPR4g, that is highly homologous to wPR4e; both encode putative vacuolar PR4 proteins (Wheatwin7 and Wheatwin5, respectively). wPR4e and wPR4f sub-family genes are induced by F. culmorum infection, by chemicals that lead to systemic acquired resistance and by wounding, showing different spatial and temporal induction pathways. In silico analysis of the 5′ untranslated regions of wPR4e and wPR4f-b revealed the presence of several abiotic and biotic stress-responsive elements. wPR4e and wPR4f-b putative promoters were fused to the β-glucuronidase (GUS) reporter gene, and transient and stable expression assays demonstrated that both are able to drive expression of GUS. Characterisation of these new PR4 genes and particularly of their 5′ untranslated regions, as well as the determination of their expression patterns, will contribute to our understanding of the responsiveness of this gene family to various stress conditions and of its role in plant defence. Walter de Gruyter Online Zeitschriften defence genes functional gene promoter gene expression PR4 genes promoter analysis SAR inducers Bertini, Laura oth Cascone, Annunziata oth Tucci, Marina oth D'Amore, Rosalinda oth Di Berardino, Iris oth Buonocore, Vincenzo oth Caporale, Carlo oth Caruso, Carla oth Enthalten in Biological chemistry Berlin [u.a.] : de Gruyter, 1996 387(2006), 8 vom: 09. Aug., Seite 1101-1111 (DE-627)NLEJ248235095 (DE-600)1466062-3 1437-4315 nnns volume:387 year:2006 number:8 day:09 month:08 pages:1101-1111 extent:11 https://doi.org/10.1515/BC.2006.136 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 387 2006 8 09 08 1101-1111 11 |
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10.1515/BC.2006.136 doi artikel_Grundlieferung.pp (DE-627)NLEJ24652166X DE-627 ger DE-627 rakwb Molecular and functional analysis of new members of the wheat PR4 gene family Walter de Gruyter 2006 11 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2006 by Walter de Gruyter Berlin New York Five new genes belonging to the pathogenesis-related (PR) 4 family have been cloned and characterised in Triticum aestivum. Two full-length genes, named wPR4e and wPR4f-b, were isolated by library screening, demonstrating the presence of a small intron only in wPR4f-b. Two other PR4 genes (wPR4f-a and wPR4f-c) were isolated by PCR, showing very high sequence identity with wPR4f-b and constituting a new sub-family. Transcription start analysis was performed by RLM-RACE, leading to the isolation of a fifth gene, named wPR4g, that is highly homologous to wPR4e; both encode putative vacuolar PR4 proteins (Wheatwin7 and Wheatwin5, respectively). wPR4e and wPR4f sub-family genes are induced by F. culmorum infection, by chemicals that lead to systemic acquired resistance and by wounding, showing different spatial and temporal induction pathways. In silico analysis of the 5′ untranslated regions of wPR4e and wPR4f-b revealed the presence of several abiotic and biotic stress-responsive elements. wPR4e and wPR4f-b putative promoters were fused to the β-glucuronidase (GUS) reporter gene, and transient and stable expression assays demonstrated that both are able to drive expression of GUS. Characterisation of these new PR4 genes and particularly of their 5′ untranslated regions, as well as the determination of their expression patterns, will contribute to our understanding of the responsiveness of this gene family to various stress conditions and of its role in plant defence. Walter de Gruyter Online Zeitschriften defence genes functional gene promoter gene expression PR4 genes promoter analysis SAR inducers Bertini, Laura oth Cascone, Annunziata oth Tucci, Marina oth D'Amore, Rosalinda oth Di Berardino, Iris oth Buonocore, Vincenzo oth Caporale, Carlo oth Caruso, Carla oth Enthalten in Biological chemistry Berlin [u.a.] : de Gruyter, 1996 387(2006), 8 vom: 09. Aug., Seite 1101-1111 (DE-627)NLEJ248235095 (DE-600)1466062-3 1437-4315 nnns volume:387 year:2006 number:8 day:09 month:08 pages:1101-1111 extent:11 https://doi.org/10.1515/BC.2006.136 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 387 2006 8 09 08 1101-1111 11 |
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10.1515/BC.2006.136 doi artikel_Grundlieferung.pp (DE-627)NLEJ24652166X DE-627 ger DE-627 rakwb Molecular and functional analysis of new members of the wheat PR4 gene family Walter de Gruyter 2006 11 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2006 by Walter de Gruyter Berlin New York Five new genes belonging to the pathogenesis-related (PR) 4 family have been cloned and characterised in Triticum aestivum. Two full-length genes, named wPR4e and wPR4f-b, were isolated by library screening, demonstrating the presence of a small intron only in wPR4f-b. Two other PR4 genes (wPR4f-a and wPR4f-c) were isolated by PCR, showing very high sequence identity with wPR4f-b and constituting a new sub-family. Transcription start analysis was performed by RLM-RACE, leading to the isolation of a fifth gene, named wPR4g, that is highly homologous to wPR4e; both encode putative vacuolar PR4 proteins (Wheatwin7 and Wheatwin5, respectively). wPR4e and wPR4f sub-family genes are induced by F. culmorum infection, by chemicals that lead to systemic acquired resistance and by wounding, showing different spatial and temporal induction pathways. In silico analysis of the 5′ untranslated regions of wPR4e and wPR4f-b revealed the presence of several abiotic and biotic stress-responsive elements. wPR4e and wPR4f-b putative promoters were fused to the β-glucuronidase (GUS) reporter gene, and transient and stable expression assays demonstrated that both are able to drive expression of GUS. Characterisation of these new PR4 genes and particularly of their 5′ untranslated regions, as well as the determination of their expression patterns, will contribute to our understanding of the responsiveness of this gene family to various stress conditions and of its role in plant defence. Walter de Gruyter Online Zeitschriften defence genes functional gene promoter gene expression PR4 genes promoter analysis SAR inducers Bertini, Laura oth Cascone, Annunziata oth Tucci, Marina oth D'Amore, Rosalinda oth Di Berardino, Iris oth Buonocore, Vincenzo oth Caporale, Carlo oth Caruso, Carla oth Enthalten in Biological chemistry Berlin [u.a.] : de Gruyter, 1996 387(2006), 8 vom: 09. Aug., Seite 1101-1111 (DE-627)NLEJ248235095 (DE-600)1466062-3 1437-4315 nnns volume:387 year:2006 number:8 day:09 month:08 pages:1101-1111 extent:11 https://doi.org/10.1515/BC.2006.136 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 387 2006 8 09 08 1101-1111 11 |
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10.1515/BC.2006.136 doi artikel_Grundlieferung.pp (DE-627)NLEJ24652166X DE-627 ger DE-627 rakwb Molecular and functional analysis of new members of the wheat PR4 gene family Walter de Gruyter 2006 11 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2006 by Walter de Gruyter Berlin New York Five new genes belonging to the pathogenesis-related (PR) 4 family have been cloned and characterised in Triticum aestivum. Two full-length genes, named wPR4e and wPR4f-b, were isolated by library screening, demonstrating the presence of a small intron only in wPR4f-b. Two other PR4 genes (wPR4f-a and wPR4f-c) were isolated by PCR, showing very high sequence identity with wPR4f-b and constituting a new sub-family. Transcription start analysis was performed by RLM-RACE, leading to the isolation of a fifth gene, named wPR4g, that is highly homologous to wPR4e; both encode putative vacuolar PR4 proteins (Wheatwin7 and Wheatwin5, respectively). wPR4e and wPR4f sub-family genes are induced by F. culmorum infection, by chemicals that lead to systemic acquired resistance and by wounding, showing different spatial and temporal induction pathways. In silico analysis of the 5′ untranslated regions of wPR4e and wPR4f-b revealed the presence of several abiotic and biotic stress-responsive elements. wPR4e and wPR4f-b putative promoters were fused to the β-glucuronidase (GUS) reporter gene, and transient and stable expression assays demonstrated that both are able to drive expression of GUS. Characterisation of these new PR4 genes and particularly of their 5′ untranslated regions, as well as the determination of their expression patterns, will contribute to our understanding of the responsiveness of this gene family to various stress conditions and of its role in plant defence. Walter de Gruyter Online Zeitschriften defence genes functional gene promoter gene expression PR4 genes promoter analysis SAR inducers Bertini, Laura oth Cascone, Annunziata oth Tucci, Marina oth D'Amore, Rosalinda oth Di Berardino, Iris oth Buonocore, Vincenzo oth Caporale, Carlo oth Caruso, Carla oth Enthalten in Biological chemistry Berlin [u.a.] : de Gruyter, 1996 387(2006), 8 vom: 09. Aug., Seite 1101-1111 (DE-627)NLEJ248235095 (DE-600)1466062-3 1437-4315 nnns volume:387 year:2006 number:8 day:09 month:08 pages:1101-1111 extent:11 https://doi.org/10.1515/BC.2006.136 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 387 2006 8 09 08 1101-1111 11 |
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Molecular and functional analysis of new members of the wheat PR4 gene family |
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Molecular and functional analysis of new members of the wheat PR4 gene family |
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10.1515/BC.2006.136 |
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molecular and functional analysis of new members of the wheat pr4 gene family |
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Molecular and functional analysis of new members of the wheat PR4 gene family |
| abstract |
Five new genes belonging to the pathogenesis-related (PR) 4 family have been cloned and characterised in Triticum aestivum. Two full-length genes, named wPR4e and wPR4f-b, were isolated by library screening, demonstrating the presence of a small intron only in wPR4f-b. Two other PR4 genes (wPR4f-a and wPR4f-c) were isolated by PCR, showing very high sequence identity with wPR4f-b and constituting a new sub-family. Transcription start analysis was performed by RLM-RACE, leading to the isolation of a fifth gene, named wPR4g, that is highly homologous to wPR4e; both encode putative vacuolar PR4 proteins (Wheatwin7 and Wheatwin5, respectively). wPR4e and wPR4f sub-family genes are induced by F. culmorum infection, by chemicals that lead to systemic acquired resistance and by wounding, showing different spatial and temporal induction pathways. In silico analysis of the 5′ untranslated regions of wPR4e and wPR4f-b revealed the presence of several abiotic and biotic stress-responsive elements. wPR4e and wPR4f-b putative promoters were fused to the β-glucuronidase (GUS) reporter gene, and transient and stable expression assays demonstrated that both are able to drive expression of GUS. Characterisation of these new PR4 genes and particularly of their 5′ untranslated regions, as well as the determination of their expression patterns, will contribute to our understanding of the responsiveness of this gene family to various stress conditions and of its role in plant defence. ©2006 by Walter de Gruyter Berlin New York |
| abstractGer |
Five new genes belonging to the pathogenesis-related (PR) 4 family have been cloned and characterised in Triticum aestivum. Two full-length genes, named wPR4e and wPR4f-b, were isolated by library screening, demonstrating the presence of a small intron only in wPR4f-b. Two other PR4 genes (wPR4f-a and wPR4f-c) were isolated by PCR, showing very high sequence identity with wPR4f-b and constituting a new sub-family. Transcription start analysis was performed by RLM-RACE, leading to the isolation of a fifth gene, named wPR4g, that is highly homologous to wPR4e; both encode putative vacuolar PR4 proteins (Wheatwin7 and Wheatwin5, respectively). wPR4e and wPR4f sub-family genes are induced by F. culmorum infection, by chemicals that lead to systemic acquired resistance and by wounding, showing different spatial and temporal induction pathways. In silico analysis of the 5′ untranslated regions of wPR4e and wPR4f-b revealed the presence of several abiotic and biotic stress-responsive elements. wPR4e and wPR4f-b putative promoters were fused to the β-glucuronidase (GUS) reporter gene, and transient and stable expression assays demonstrated that both are able to drive expression of GUS. Characterisation of these new PR4 genes and particularly of their 5′ untranslated regions, as well as the determination of their expression patterns, will contribute to our understanding of the responsiveness of this gene family to various stress conditions and of its role in plant defence. ©2006 by Walter de Gruyter Berlin New York |
| abstract_unstemmed |
Five new genes belonging to the pathogenesis-related (PR) 4 family have been cloned and characterised in Triticum aestivum. Two full-length genes, named wPR4e and wPR4f-b, were isolated by library screening, demonstrating the presence of a small intron only in wPR4f-b. Two other PR4 genes (wPR4f-a and wPR4f-c) were isolated by PCR, showing very high sequence identity with wPR4f-b and constituting a new sub-family. Transcription start analysis was performed by RLM-RACE, leading to the isolation of a fifth gene, named wPR4g, that is highly homologous to wPR4e; both encode putative vacuolar PR4 proteins (Wheatwin7 and Wheatwin5, respectively). wPR4e and wPR4f sub-family genes are induced by F. culmorum infection, by chemicals that lead to systemic acquired resistance and by wounding, showing different spatial and temporal induction pathways. In silico analysis of the 5′ untranslated regions of wPR4e and wPR4f-b revealed the presence of several abiotic and biotic stress-responsive elements. wPR4e and wPR4f-b putative promoters were fused to the β-glucuronidase (GUS) reporter gene, and transient and stable expression assays demonstrated that both are able to drive expression of GUS. Characterisation of these new PR4 genes and particularly of their 5′ untranslated regions, as well as the determination of their expression patterns, will contribute to our understanding of the responsiveness of this gene family to various stress conditions and of its role in plant defence. ©2006 by Walter de Gruyter Berlin New York |
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Molecular and functional analysis of new members of the wheat PR4 gene family |
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Bertini, Laura Cascone, Annunziata Tucci, Marina D'Amore, Rosalinda Di Berardino, Iris Buonocore, Vincenzo Caporale, Carlo Caruso, Carla |
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Bertini, Laura Cascone, Annunziata Tucci, Marina D'Amore, Rosalinda Di Berardino, Iris Buonocore, Vincenzo Caporale, Carlo Caruso, Carla |
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