Hormone-autonomous cell culture from cotydelonary tissue of the marine plant Cymodocea nodosa
In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymati...
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E-Artikel |
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| Erschienen: |
De Gruyter ; 2012 |
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10 |
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| Reproduktion: |
Walter de Gruyter Online Zeitschriften |
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| Übergeordnetes Werk: |
Enthalten in: Botanica marina - Berlin [u.a.] : de Gruyter, 1959, 55(2012), 6 vom: 04. Sept., Seite 591-600 |
| Übergeordnetes Werk: |
volume:55 ; year:2012 ; number:6 ; day:04 ; month:09 ; pages:591-600 ; extent:10 |
| Links: |
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| DOI / URN: |
10.1515/bot-2012-0122 |
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NLEJ246647140 |
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| 520 | |a In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potential. An effective enzymatic digestion protocol for C. nodosa meristem tissue was developed; this consisted of a 24-h digestion period at 28°C using a 0.6-m enzymatic solution (plasmolysis medium containing 1% (w:v) cellulase and hemicellulase). It was necessary to add citric acid (0.02 m) as an antioxidant to the enzymatic solution to obtain the highest cell yield and viability (mean±SE: 371.6±30.1×105 cells g-1 fw and 97.8±0.6%, respectively). NaCl and other salt components of the seawater were key factors in the establishment of axenic cell cultures and their further progress; their absence in the medium inhibited contaminant overgrowth. Nevertheless, cells maintained growth for 90 days, even without hormones and deprived of salts, although these salts might be necessary to allow the development of morphogenetic programmes desired for plant production. In this manner, we generated a basic tool for the future of C. nodosa in vitro culture that will allow the establishment and maintenance of hormone-autonomous cell cultures with strong potential in future biotechnology for marine higher plants. | ||
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10.1515/bot-2012-0122 doi artikel_Grundlieferung.pp (DE-627)NLEJ246647140 DE-627 ger DE-627 rakwb Hormone-autonomous cell culture from cotydelonary tissue of the marine plant Cymodocea nodosa De Gruyter 2012 10 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potential. An effective enzymatic digestion protocol for C. nodosa meristem tissue was developed; this consisted of a 24-h digestion period at 28°C using a 0.6-m enzymatic solution (plasmolysis medium containing 1% (w:v) cellulase and hemicellulase). It was necessary to add citric acid (0.02 m) as an antioxidant to the enzymatic solution to obtain the highest cell yield and viability (mean±SE: 371.6±30.1×105 cells g-1 fw and 97.8±0.6%, respectively). NaCl and other salt components of the seawater were key factors in the establishment of axenic cell cultures and their further progress; their absence in the medium inhibited contaminant overgrowth. Nevertheless, cells maintained growth for 90 days, even without hormones and deprived of salts, although these salts might be necessary to allow the development of morphogenetic programmes desired for plant production. In this manner, we generated a basic tool for the future of C. nodosa in vitro culture that will allow the establishment and maintenance of hormone-autonomous cell cultures with strong potential in future biotechnology for marine higher plants. Walter de Gruyter Online Zeitschriften axenic cells cotyledon culture Cymodocea nodosa Elso, Maite Zarranz oth González-Henríquez, Nieves oth García-Jiménez, Pilar oth Robaina, Rafael R. oth Enthalten in Botanica marina Berlin [u.a.] : de Gruyter, 1959 55(2012), 6 vom: 04. Sept., Seite 591-600 (DE-627)NLEJ248235184 (DE-600)1475447-2 1437-4323 nnns volume:55 year:2012 number:6 day:04 month:09 pages:591-600 extent:10 https://doi.org/10.1515/bot-2012-0122 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 55 2012 6 04 09 591-600 10 |
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10.1515/bot-2012-0122 doi artikel_Grundlieferung.pp (DE-627)NLEJ246647140 DE-627 ger DE-627 rakwb Hormone-autonomous cell culture from cotydelonary tissue of the marine plant Cymodocea nodosa De Gruyter 2012 10 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potential. An effective enzymatic digestion protocol for C. nodosa meristem tissue was developed; this consisted of a 24-h digestion period at 28°C using a 0.6-m enzymatic solution (plasmolysis medium containing 1% (w:v) cellulase and hemicellulase). It was necessary to add citric acid (0.02 m) as an antioxidant to the enzymatic solution to obtain the highest cell yield and viability (mean±SE: 371.6±30.1×105 cells g-1 fw and 97.8±0.6%, respectively). NaCl and other salt components of the seawater were key factors in the establishment of axenic cell cultures and their further progress; their absence in the medium inhibited contaminant overgrowth. Nevertheless, cells maintained growth for 90 days, even without hormones and deprived of salts, although these salts might be necessary to allow the development of morphogenetic programmes desired for plant production. In this manner, we generated a basic tool for the future of C. nodosa in vitro culture that will allow the establishment and maintenance of hormone-autonomous cell cultures with strong potential in future biotechnology for marine higher plants. Walter de Gruyter Online Zeitschriften axenic cells cotyledon culture Cymodocea nodosa Elso, Maite Zarranz oth González-Henríquez, Nieves oth García-Jiménez, Pilar oth Robaina, Rafael R. oth Enthalten in Botanica marina Berlin [u.a.] : de Gruyter, 1959 55(2012), 6 vom: 04. Sept., Seite 591-600 (DE-627)NLEJ248235184 (DE-600)1475447-2 1437-4323 nnns volume:55 year:2012 number:6 day:04 month:09 pages:591-600 extent:10 https://doi.org/10.1515/bot-2012-0122 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 55 2012 6 04 09 591-600 10 |
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10.1515/bot-2012-0122 doi artikel_Grundlieferung.pp (DE-627)NLEJ246647140 DE-627 ger DE-627 rakwb Hormone-autonomous cell culture from cotydelonary tissue of the marine plant Cymodocea nodosa De Gruyter 2012 10 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potential. An effective enzymatic digestion protocol for C. nodosa meristem tissue was developed; this consisted of a 24-h digestion period at 28°C using a 0.6-m enzymatic solution (plasmolysis medium containing 1% (w:v) cellulase and hemicellulase). It was necessary to add citric acid (0.02 m) as an antioxidant to the enzymatic solution to obtain the highest cell yield and viability (mean±SE: 371.6±30.1×105 cells g-1 fw and 97.8±0.6%, respectively). NaCl and other salt components of the seawater were key factors in the establishment of axenic cell cultures and their further progress; their absence in the medium inhibited contaminant overgrowth. Nevertheless, cells maintained growth for 90 days, even without hormones and deprived of salts, although these salts might be necessary to allow the development of morphogenetic programmes desired for plant production. In this manner, we generated a basic tool for the future of C. nodosa in vitro culture that will allow the establishment and maintenance of hormone-autonomous cell cultures with strong potential in future biotechnology for marine higher plants. Walter de Gruyter Online Zeitschriften axenic cells cotyledon culture Cymodocea nodosa Elso, Maite Zarranz oth González-Henríquez, Nieves oth García-Jiménez, Pilar oth Robaina, Rafael R. oth Enthalten in Botanica marina Berlin [u.a.] : de Gruyter, 1959 55(2012), 6 vom: 04. Sept., Seite 591-600 (DE-627)NLEJ248235184 (DE-600)1475447-2 1437-4323 nnns volume:55 year:2012 number:6 day:04 month:09 pages:591-600 extent:10 https://doi.org/10.1515/bot-2012-0122 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 55 2012 6 04 09 591-600 10 |
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10.1515/bot-2012-0122 doi artikel_Grundlieferung.pp (DE-627)NLEJ246647140 DE-627 ger DE-627 rakwb Hormone-autonomous cell culture from cotydelonary tissue of the marine plant Cymodocea nodosa De Gruyter 2012 10 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potential. An effective enzymatic digestion protocol for C. nodosa meristem tissue was developed; this consisted of a 24-h digestion period at 28°C using a 0.6-m enzymatic solution (plasmolysis medium containing 1% (w:v) cellulase and hemicellulase). It was necessary to add citric acid (0.02 m) as an antioxidant to the enzymatic solution to obtain the highest cell yield and viability (mean±SE: 371.6±30.1×105 cells g-1 fw and 97.8±0.6%, respectively). NaCl and other salt components of the seawater were key factors in the establishment of axenic cell cultures and their further progress; their absence in the medium inhibited contaminant overgrowth. Nevertheless, cells maintained growth for 90 days, even without hormones and deprived of salts, although these salts might be necessary to allow the development of morphogenetic programmes desired for plant production. In this manner, we generated a basic tool for the future of C. nodosa in vitro culture that will allow the establishment and maintenance of hormone-autonomous cell cultures with strong potential in future biotechnology for marine higher plants. Walter de Gruyter Online Zeitschriften axenic cells cotyledon culture Cymodocea nodosa Elso, Maite Zarranz oth González-Henríquez, Nieves oth García-Jiménez, Pilar oth Robaina, Rafael R. oth Enthalten in Botanica marina Berlin [u.a.] : de Gruyter, 1959 55(2012), 6 vom: 04. Sept., Seite 591-600 (DE-627)NLEJ248235184 (DE-600)1475447-2 1437-4323 nnns volume:55 year:2012 number:6 day:04 month:09 pages:591-600 extent:10 https://doi.org/10.1515/bot-2012-0122 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 55 2012 6 04 09 591-600 10 |
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10.1515/bot-2012-0122 doi artikel_Grundlieferung.pp (DE-627)NLEJ246647140 DE-627 ger DE-627 rakwb Hormone-autonomous cell culture from cotydelonary tissue of the marine plant Cymodocea nodosa De Gruyter 2012 10 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potential. An effective enzymatic digestion protocol for C. nodosa meristem tissue was developed; this consisted of a 24-h digestion period at 28°C using a 0.6-m enzymatic solution (plasmolysis medium containing 1% (w:v) cellulase and hemicellulase). It was necessary to add citric acid (0.02 m) as an antioxidant to the enzymatic solution to obtain the highest cell yield and viability (mean±SE: 371.6±30.1×105 cells g-1 fw and 97.8±0.6%, respectively). NaCl and other salt components of the seawater were key factors in the establishment of axenic cell cultures and their further progress; their absence in the medium inhibited contaminant overgrowth. Nevertheless, cells maintained growth for 90 days, even without hormones and deprived of salts, although these salts might be necessary to allow the development of morphogenetic programmes desired for plant production. In this manner, we generated a basic tool for the future of C. nodosa in vitro culture that will allow the establishment and maintenance of hormone-autonomous cell cultures with strong potential in future biotechnology for marine higher plants. Walter de Gruyter Online Zeitschriften axenic cells cotyledon culture Cymodocea nodosa Elso, Maite Zarranz oth González-Henríquez, Nieves oth García-Jiménez, Pilar oth Robaina, Rafael R. oth Enthalten in Botanica marina Berlin [u.a.] : de Gruyter, 1959 55(2012), 6 vom: 04. Sept., Seite 591-600 (DE-627)NLEJ248235184 (DE-600)1475447-2 1437-4323 nnns volume:55 year:2012 number:6 day:04 month:09 pages:591-600 extent:10 https://doi.org/10.1515/bot-2012-0122 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 55 2012 6 04 09 591-600 10 |
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hormone-autonomous cell culture from cotydelonary tissue of the marine plant cymodocea nodosa |
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Hormone-autonomous cell culture from cotydelonary tissue of the marine plant Cymodocea nodosa |
| abstract |
In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potential. An effective enzymatic digestion protocol for C. nodosa meristem tissue was developed; this consisted of a 24-h digestion period at 28°C using a 0.6-m enzymatic solution (plasmolysis medium containing 1% (w:v) cellulase and hemicellulase). It was necessary to add citric acid (0.02 m) as an antioxidant to the enzymatic solution to obtain the highest cell yield and viability (mean±SE: 371.6±30.1×105 cells g-1 fw and 97.8±0.6%, respectively). NaCl and other salt components of the seawater were key factors in the establishment of axenic cell cultures and their further progress; their absence in the medium inhibited contaminant overgrowth. Nevertheless, cells maintained growth for 90 days, even without hormones and deprived of salts, although these salts might be necessary to allow the development of morphogenetic programmes desired for plant production. In this manner, we generated a basic tool for the future of C. nodosa in vitro culture that will allow the establishment and maintenance of hormone-autonomous cell cultures with strong potential in future biotechnology for marine higher plants. |
| abstractGer |
In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potential. An effective enzymatic digestion protocol for C. nodosa meristem tissue was developed; this consisted of a 24-h digestion period at 28°C using a 0.6-m enzymatic solution (plasmolysis medium containing 1% (w:v) cellulase and hemicellulase). It was necessary to add citric acid (0.02 m) as an antioxidant to the enzymatic solution to obtain the highest cell yield and viability (mean±SE: 371.6±30.1×105 cells g-1 fw and 97.8±0.6%, respectively). NaCl and other salt components of the seawater were key factors in the establishment of axenic cell cultures and their further progress; their absence in the medium inhibited contaminant overgrowth. Nevertheless, cells maintained growth for 90 days, even without hormones and deprived of salts, although these salts might be necessary to allow the development of morphogenetic programmes desired for plant production. In this manner, we generated a basic tool for the future of C. nodosa in vitro culture that will allow the establishment and maintenance of hormone-autonomous cell cultures with strong potential in future biotechnology for marine higher plants. |
| abstract_unstemmed |
In vitro germinated seedlings of Cymodocea nodosa were used as a source of young embryonic tissue for cell culture. Histological study of the cotyledon revealed the presence of meristematic tissue that gave rise to the first leaf of the plantlet, which released large numbers of cells after enzymatic digestion and thus indicated great growth potential. An effective enzymatic digestion protocol for C. nodosa meristem tissue was developed; this consisted of a 24-h digestion period at 28°C using a 0.6-m enzymatic solution (plasmolysis medium containing 1% (w:v) cellulase and hemicellulase). It was necessary to add citric acid (0.02 m) as an antioxidant to the enzymatic solution to obtain the highest cell yield and viability (mean±SE: 371.6±30.1×105 cells g-1 fw and 97.8±0.6%, respectively). NaCl and other salt components of the seawater were key factors in the establishment of axenic cell cultures and their further progress; their absence in the medium inhibited contaminant overgrowth. Nevertheless, cells maintained growth for 90 days, even without hormones and deprived of salts, although these salts might be necessary to allow the development of morphogenetic programmes desired for plant production. In this manner, we generated a basic tool for the future of C. nodosa in vitro culture that will allow the establishment and maintenance of hormone-autonomous cell cultures with strong potential in future biotechnology for marine higher plants. |
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| title_short |
Hormone-autonomous cell culture from cotydelonary tissue of the marine plant Cymodocea nodosa |
| url |
https://doi.org/10.1515/bot-2012-0122 |
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| author2 |
Elso, Maite Zarranz González-Henríquez, Nieves García-Jiménez, Pilar Robaina, Rafael R. |
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Elso, Maite Zarranz González-Henríquez, Nieves García-Jiménez, Pilar Robaina, Rafael R. |
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NLEJ248235184 |
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| doi_str |
10.1515/bot-2012-0122 |
| up_date |
2024-07-06T08:59:09.856Z |
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7.3998127 |