Performance evaluation of human cytokines profiles obtained by various multiplexed-based technologies underlines a need for standardization
Background: Multiplexed methods permit simultaneous quantification of multiple cytokines. As several manufacturers offer reagents to quantify the same cytokines on a single instrument, comparison of the distribution should be made to determine whether these data are comparable from one assay to anot...
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E-Artikel |
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De Gruyter ; 2013 |
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9 |
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Walter de Gruyter Online Zeitschriften |
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Enthalten in: Clinical chemistry and laboratory medicine - Berlin [u.a.] : De Gruyter, 1998, 51(2013), 7 vom: 09. Jan., Seite 1385-1393 |
| Übergeordnetes Werk: |
volume:51 ; year:2013 ; number:7 ; day:09 ; month:01 ; pages:1385-1393 ; extent:9 |
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| DOI / URN: |
10.1515/cclm-2012-0648 |
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NLEJ246694971 |
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| 520 | |a Background: Multiplexed methods permit simultaneous quantification of multiple cytokines. As several manufacturers offer reagents to quantify the same cytokines on a single instrument, comparison of the distribution should be made to determine whether these data are comparable from one assay to another. Methods: We performed the quantification of cytokines in serum samples with three commercially available assays: Cytometric Bead Array (CBA), Protein Biochip Array Technology (PBAT), and Luminex Technology analysis. Using detection limit and reference range of the three commercial multiplex technologies, we evaluated: 1) the overall distribution of cytokines; and 2) the clinical impact. Results: The three cytokines, IL-1β, IL-1α and IL-4, cannot be measured by these methods because of the high number of non-detected data (>50%). By contrast, four cytokines as IL-8, VEGF, MCP-1 and EGF exhibited a low percentage of non-detected data whatever method was used. The comparison of the percentage of samples with values higher than the respective reference range of each method reported an absence of clinical concordance (Cohen’s κ-test <0.40). Conclusions: Our results highlight the lack of transferability between the three commercially available multiplex methods evaluated (CBA, PBAT and Luminex Technology). Analytical performances are adequate for longitudinal studies using a same methodology but caution should be used for comparisons between results obtained with different methods underlying a need for standardization. | ||
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| 700 | 1 | |a Lehmann, Sylvain |4 oth | |
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| 700 | 1 | |a Cristol, Jean Paul |4 oth | |
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10.1515/cclm-2012-0648 doi artikel_Grundlieferung.pp (DE-627)NLEJ246694971 DE-627 ger DE-627 rakwb Performance evaluation of human cytokines profiles obtained by various multiplexed-based technologies underlines a need for standardization De Gruyter 2013 9 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Multiplexed methods permit simultaneous quantification of multiple cytokines. As several manufacturers offer reagents to quantify the same cytokines on a single instrument, comparison of the distribution should be made to determine whether these data are comparable from one assay to another. Methods: We performed the quantification of cytokines in serum samples with three commercially available assays: Cytometric Bead Array (CBA), Protein Biochip Array Technology (PBAT), and Luminex Technology analysis. Using detection limit and reference range of the three commercial multiplex technologies, we evaluated: 1) the overall distribution of cytokines; and 2) the clinical impact. Results: The three cytokines, IL-1β, IL-1α and IL-4, cannot be measured by these methods because of the high number of non-detected data (>50%). By contrast, four cytokines as IL-8, VEGF, MCP-1 and EGF exhibited a low percentage of non-detected data whatever method was used. The comparison of the percentage of samples with values higher than the respective reference range of each method reported an absence of clinical concordance (Cohen’s κ-test <0.40). Conclusions: Our results highlight the lack of transferability between the three commercially available multiplex methods evaluated (CBA, PBAT and Luminex Technology). Analytical performances are adequate for longitudinal studies using a same methodology but caution should be used for comparisons between results obtained with different methods underlying a need for standardization. Walter de Gruyter Online Zeitschriften cytokines Cytometric Bead Array (CBA) Luminex Technology multiplex-based immunoassays Protein Biochip Array Technology (PBAT) Dupuy, Anne Marie oth Kuster, Nils oth Lizard, Gérard oth Ragot, Kévin oth Lehmann, Sylvain oth Gallix, Benoît oth Cristol, Jean Paul oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 51(2013), 7 vom: 09. Jan., Seite 1385-1393 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:51 year:2013 number:7 day:09 month:01 pages:1385-1393 extent:9 https://doi.org/10.1515/cclm-2012-0648 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 51 2013 7 09 01 1385-1393 9 |
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10.1515/cclm-2012-0648 doi artikel_Grundlieferung.pp (DE-627)NLEJ246694971 DE-627 ger DE-627 rakwb Performance evaluation of human cytokines profiles obtained by various multiplexed-based technologies underlines a need for standardization De Gruyter 2013 9 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Multiplexed methods permit simultaneous quantification of multiple cytokines. As several manufacturers offer reagents to quantify the same cytokines on a single instrument, comparison of the distribution should be made to determine whether these data are comparable from one assay to another. Methods: We performed the quantification of cytokines in serum samples with three commercially available assays: Cytometric Bead Array (CBA), Protein Biochip Array Technology (PBAT), and Luminex Technology analysis. Using detection limit and reference range of the three commercial multiplex technologies, we evaluated: 1) the overall distribution of cytokines; and 2) the clinical impact. Results: The three cytokines, IL-1β, IL-1α and IL-4, cannot be measured by these methods because of the high number of non-detected data (>50%). By contrast, four cytokines as IL-8, VEGF, MCP-1 and EGF exhibited a low percentage of non-detected data whatever method was used. The comparison of the percentage of samples with values higher than the respective reference range of each method reported an absence of clinical concordance (Cohen’s κ-test <0.40). Conclusions: Our results highlight the lack of transferability between the three commercially available multiplex methods evaluated (CBA, PBAT and Luminex Technology). Analytical performances are adequate for longitudinal studies using a same methodology but caution should be used for comparisons between results obtained with different methods underlying a need for standardization. Walter de Gruyter Online Zeitschriften cytokines Cytometric Bead Array (CBA) Luminex Technology multiplex-based immunoassays Protein Biochip Array Technology (PBAT) Dupuy, Anne Marie oth Kuster, Nils oth Lizard, Gérard oth Ragot, Kévin oth Lehmann, Sylvain oth Gallix, Benoît oth Cristol, Jean Paul oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 51(2013), 7 vom: 09. Jan., Seite 1385-1393 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:51 year:2013 number:7 day:09 month:01 pages:1385-1393 extent:9 https://doi.org/10.1515/cclm-2012-0648 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 51 2013 7 09 01 1385-1393 9 |
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10.1515/cclm-2012-0648 doi artikel_Grundlieferung.pp (DE-627)NLEJ246694971 DE-627 ger DE-627 rakwb Performance evaluation of human cytokines profiles obtained by various multiplexed-based technologies underlines a need for standardization De Gruyter 2013 9 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Multiplexed methods permit simultaneous quantification of multiple cytokines. As several manufacturers offer reagents to quantify the same cytokines on a single instrument, comparison of the distribution should be made to determine whether these data are comparable from one assay to another. Methods: We performed the quantification of cytokines in serum samples with three commercially available assays: Cytometric Bead Array (CBA), Protein Biochip Array Technology (PBAT), and Luminex Technology analysis. Using detection limit and reference range of the three commercial multiplex technologies, we evaluated: 1) the overall distribution of cytokines; and 2) the clinical impact. Results: The three cytokines, IL-1β, IL-1α and IL-4, cannot be measured by these methods because of the high number of non-detected data (>50%). By contrast, four cytokines as IL-8, VEGF, MCP-1 and EGF exhibited a low percentage of non-detected data whatever method was used. The comparison of the percentage of samples with values higher than the respective reference range of each method reported an absence of clinical concordance (Cohen’s κ-test <0.40). Conclusions: Our results highlight the lack of transferability between the three commercially available multiplex methods evaluated (CBA, PBAT and Luminex Technology). Analytical performances are adequate for longitudinal studies using a same methodology but caution should be used for comparisons between results obtained with different methods underlying a need for standardization. Walter de Gruyter Online Zeitschriften cytokines Cytometric Bead Array (CBA) Luminex Technology multiplex-based immunoassays Protein Biochip Array Technology (PBAT) Dupuy, Anne Marie oth Kuster, Nils oth Lizard, Gérard oth Ragot, Kévin oth Lehmann, Sylvain oth Gallix, Benoît oth Cristol, Jean Paul oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 51(2013), 7 vom: 09. Jan., Seite 1385-1393 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:51 year:2013 number:7 day:09 month:01 pages:1385-1393 extent:9 https://doi.org/10.1515/cclm-2012-0648 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 51 2013 7 09 01 1385-1393 9 |
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10.1515/cclm-2012-0648 doi artikel_Grundlieferung.pp (DE-627)NLEJ246694971 DE-627 ger DE-627 rakwb Performance evaluation of human cytokines profiles obtained by various multiplexed-based technologies underlines a need for standardization De Gruyter 2013 9 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Multiplexed methods permit simultaneous quantification of multiple cytokines. As several manufacturers offer reagents to quantify the same cytokines on a single instrument, comparison of the distribution should be made to determine whether these data are comparable from one assay to another. Methods: We performed the quantification of cytokines in serum samples with three commercially available assays: Cytometric Bead Array (CBA), Protein Biochip Array Technology (PBAT), and Luminex Technology analysis. Using detection limit and reference range of the three commercial multiplex technologies, we evaluated: 1) the overall distribution of cytokines; and 2) the clinical impact. Results: The three cytokines, IL-1β, IL-1α and IL-4, cannot be measured by these methods because of the high number of non-detected data (>50%). By contrast, four cytokines as IL-8, VEGF, MCP-1 and EGF exhibited a low percentage of non-detected data whatever method was used. The comparison of the percentage of samples with values higher than the respective reference range of each method reported an absence of clinical concordance (Cohen’s κ-test <0.40). Conclusions: Our results highlight the lack of transferability between the three commercially available multiplex methods evaluated (CBA, PBAT and Luminex Technology). Analytical performances are adequate for longitudinal studies using a same methodology but caution should be used for comparisons between results obtained with different methods underlying a need for standardization. Walter de Gruyter Online Zeitschriften cytokines Cytometric Bead Array (CBA) Luminex Technology multiplex-based immunoassays Protein Biochip Array Technology (PBAT) Dupuy, Anne Marie oth Kuster, Nils oth Lizard, Gérard oth Ragot, Kévin oth Lehmann, Sylvain oth Gallix, Benoît oth Cristol, Jean Paul oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 51(2013), 7 vom: 09. Jan., Seite 1385-1393 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:51 year:2013 number:7 day:09 month:01 pages:1385-1393 extent:9 https://doi.org/10.1515/cclm-2012-0648 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 51 2013 7 09 01 1385-1393 9 |
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10.1515/cclm-2012-0648 doi artikel_Grundlieferung.pp (DE-627)NLEJ246694971 DE-627 ger DE-627 rakwb Performance evaluation of human cytokines profiles obtained by various multiplexed-based technologies underlines a need for standardization De Gruyter 2013 9 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Multiplexed methods permit simultaneous quantification of multiple cytokines. As several manufacturers offer reagents to quantify the same cytokines on a single instrument, comparison of the distribution should be made to determine whether these data are comparable from one assay to another. Methods: We performed the quantification of cytokines in serum samples with three commercially available assays: Cytometric Bead Array (CBA), Protein Biochip Array Technology (PBAT), and Luminex Technology analysis. Using detection limit and reference range of the three commercial multiplex technologies, we evaluated: 1) the overall distribution of cytokines; and 2) the clinical impact. Results: The three cytokines, IL-1β, IL-1α and IL-4, cannot be measured by these methods because of the high number of non-detected data (>50%). By contrast, four cytokines as IL-8, VEGF, MCP-1 and EGF exhibited a low percentage of non-detected data whatever method was used. The comparison of the percentage of samples with values higher than the respective reference range of each method reported an absence of clinical concordance (Cohen’s κ-test <0.40). Conclusions: Our results highlight the lack of transferability between the three commercially available multiplex methods evaluated (CBA, PBAT and Luminex Technology). Analytical performances are adequate for longitudinal studies using a same methodology but caution should be used for comparisons between results obtained with different methods underlying a need for standardization. Walter de Gruyter Online Zeitschriften cytokines Cytometric Bead Array (CBA) Luminex Technology multiplex-based immunoassays Protein Biochip Array Technology (PBAT) Dupuy, Anne Marie oth Kuster, Nils oth Lizard, Gérard oth Ragot, Kévin oth Lehmann, Sylvain oth Gallix, Benoît oth Cristol, Jean Paul oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 51(2013), 7 vom: 09. Jan., Seite 1385-1393 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:51 year:2013 number:7 day:09 month:01 pages:1385-1393 extent:9 https://doi.org/10.1515/cclm-2012-0648 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 51 2013 7 09 01 1385-1393 9 |
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performance evaluation of human cytokines profiles obtained by various multiplexed-based technologies underlines a need for standardization |
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Performance evaluation of human cytokines profiles obtained by various multiplexed-based technologies underlines a need for standardization |
| abstract |
Background: Multiplexed methods permit simultaneous quantification of multiple cytokines. As several manufacturers offer reagents to quantify the same cytokines on a single instrument, comparison of the distribution should be made to determine whether these data are comparable from one assay to another. Methods: We performed the quantification of cytokines in serum samples with three commercially available assays: Cytometric Bead Array (CBA), Protein Biochip Array Technology (PBAT), and Luminex Technology analysis. Using detection limit and reference range of the three commercial multiplex technologies, we evaluated: 1) the overall distribution of cytokines; and 2) the clinical impact. Results: The three cytokines, IL-1β, IL-1α and IL-4, cannot be measured by these methods because of the high number of non-detected data (>50%). By contrast, four cytokines as IL-8, VEGF, MCP-1 and EGF exhibited a low percentage of non-detected data whatever method was used. The comparison of the percentage of samples with values higher than the respective reference range of each method reported an absence of clinical concordance (Cohen’s κ-test <0.40). Conclusions: Our results highlight the lack of transferability between the three commercially available multiplex methods evaluated (CBA, PBAT and Luminex Technology). Analytical performances are adequate for longitudinal studies using a same methodology but caution should be used for comparisons between results obtained with different methods underlying a need for standardization. |
| abstractGer |
Background: Multiplexed methods permit simultaneous quantification of multiple cytokines. As several manufacturers offer reagents to quantify the same cytokines on a single instrument, comparison of the distribution should be made to determine whether these data are comparable from one assay to another. Methods: We performed the quantification of cytokines in serum samples with three commercially available assays: Cytometric Bead Array (CBA), Protein Biochip Array Technology (PBAT), and Luminex Technology analysis. Using detection limit and reference range of the three commercial multiplex technologies, we evaluated: 1) the overall distribution of cytokines; and 2) the clinical impact. Results: The three cytokines, IL-1β, IL-1α and IL-4, cannot be measured by these methods because of the high number of non-detected data (>50%). By contrast, four cytokines as IL-8, VEGF, MCP-1 and EGF exhibited a low percentage of non-detected data whatever method was used. The comparison of the percentage of samples with values higher than the respective reference range of each method reported an absence of clinical concordance (Cohen’s κ-test <0.40). Conclusions: Our results highlight the lack of transferability between the three commercially available multiplex methods evaluated (CBA, PBAT and Luminex Technology). Analytical performances are adequate for longitudinal studies using a same methodology but caution should be used for comparisons between results obtained with different methods underlying a need for standardization. |
| abstract_unstemmed |
Background: Multiplexed methods permit simultaneous quantification of multiple cytokines. As several manufacturers offer reagents to quantify the same cytokines on a single instrument, comparison of the distribution should be made to determine whether these data are comparable from one assay to another. Methods: We performed the quantification of cytokines in serum samples with three commercially available assays: Cytometric Bead Array (CBA), Protein Biochip Array Technology (PBAT), and Luminex Technology analysis. Using detection limit and reference range of the three commercial multiplex technologies, we evaluated: 1) the overall distribution of cytokines; and 2) the clinical impact. Results: The three cytokines, IL-1β, IL-1α and IL-4, cannot be measured by these methods because of the high number of non-detected data (>50%). By contrast, four cytokines as IL-8, VEGF, MCP-1 and EGF exhibited a low percentage of non-detected data whatever method was used. The comparison of the percentage of samples with values higher than the respective reference range of each method reported an absence of clinical concordance (Cohen’s κ-test <0.40). Conclusions: Our results highlight the lack of transferability between the three commercially available multiplex methods evaluated (CBA, PBAT and Luminex Technology). Analytical performances are adequate for longitudinal studies using a same methodology but caution should be used for comparisons between results obtained with different methods underlying a need for standardization. |
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| title_short |
Performance evaluation of human cytokines profiles obtained by various multiplexed-based technologies underlines a need for standardization |
| url |
https://doi.org/10.1515/cclm-2012-0648 |
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| author2 |
Dupuy, Anne Marie Kuster, Nils Lizard, Gérard Ragot, Kévin Lehmann, Sylvain Gallix, Benoît Cristol, Jean Paul |
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Dupuy, Anne Marie Kuster, Nils Lizard, Gérard Ragot, Kévin Lehmann, Sylvain Gallix, Benoît Cristol, Jean Paul |
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NLEJ248235222 |
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| doi_str |
10.1515/cclm-2012-0648 |
| up_date |
2024-07-06T09:05:27.577Z |
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