Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR
Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Resul...
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Walter de Gruyter ; 2005 |
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Copyright © 1999 by Walter de Gruyter GmbH & Co. KG |
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Walter de Gruyter Online Zeitschriften |
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In: 36(2005), 8 vom: 01. Juni, Seite 637-639 volume:36 ; year:2005 ; number:8 ; day:01 ; month:06 ; pages:637-639 ; extent:3 |
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10.1515/CCLM.1998.112 |
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| 520 | |a Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix, the patient specific T-cell receptor γ rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor γ gene rearrangement. Three patients exhibited two rearranged T-cell receptor γ genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy. | ||
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10.1515/CCLM.1998.112 doi artikel_Grundlieferung.pp (DE-627)NLEJ246698519 DE-627 ger DE-627 rakwb Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR Walter de Gruyter 2005 3 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Copyright © 1999 by Walter de Gruyter GmbH & Co. KG Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix, the patient specific T-cell receptor γ rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor γ gene rearrangement. Three patients exhibited two rearranged T-cell receptor γ genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy. Walter de Gruyter Online Zeitschriften Zadro, Renata oth Sučić, Mirna oth Aurer, Igor oth Metelko-Kovačević, Jasminka oth Labar, Boris oth Stavljenić Rukavina, Ana oth In 36(2005), 8 vom: 01. Juni, Seite 637-639 volume:36 year:2005 number:8 day:01 month:06 pages:637-639 extent:3 https://doi.org/10.1515/CCLM.1998.112 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 36 2005 8 01 06 637-639 3 |
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10.1515/CCLM.1998.112 doi artikel_Grundlieferung.pp (DE-627)NLEJ246698519 DE-627 ger DE-627 rakwb Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR Walter de Gruyter 2005 3 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Copyright © 1999 by Walter de Gruyter GmbH & Co. KG Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix, the patient specific T-cell receptor γ rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor γ gene rearrangement. Three patients exhibited two rearranged T-cell receptor γ genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy. Walter de Gruyter Online Zeitschriften Zadro, Renata oth Sučić, Mirna oth Aurer, Igor oth Metelko-Kovačević, Jasminka oth Labar, Boris oth Stavljenić Rukavina, Ana oth In 36(2005), 8 vom: 01. Juni, Seite 637-639 volume:36 year:2005 number:8 day:01 month:06 pages:637-639 extent:3 https://doi.org/10.1515/CCLM.1998.112 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 36 2005 8 01 06 637-639 3 |
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10.1515/CCLM.1998.112 doi artikel_Grundlieferung.pp (DE-627)NLEJ246698519 DE-627 ger DE-627 rakwb Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR Walter de Gruyter 2005 3 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Copyright © 1999 by Walter de Gruyter GmbH & Co. KG Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix, the patient specific T-cell receptor γ rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor γ gene rearrangement. Three patients exhibited two rearranged T-cell receptor γ genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy. Walter de Gruyter Online Zeitschriften Zadro, Renata oth Sučić, Mirna oth Aurer, Igor oth Metelko-Kovačević, Jasminka oth Labar, Boris oth Stavljenić Rukavina, Ana oth In 36(2005), 8 vom: 01. Juni, Seite 637-639 volume:36 year:2005 number:8 day:01 month:06 pages:637-639 extent:3 https://doi.org/10.1515/CCLM.1998.112 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 36 2005 8 01 06 637-639 3 |
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10.1515/CCLM.1998.112 doi artikel_Grundlieferung.pp (DE-627)NLEJ246698519 DE-627 ger DE-627 rakwb Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR Walter de Gruyter 2005 3 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Copyright © 1999 by Walter de Gruyter GmbH & Co. KG Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix, the patient specific T-cell receptor γ rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor γ gene rearrangement. Three patients exhibited two rearranged T-cell receptor γ genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy. Walter de Gruyter Online Zeitschriften Zadro, Renata oth Sučić, Mirna oth Aurer, Igor oth Metelko-Kovačević, Jasminka oth Labar, Boris oth Stavljenić Rukavina, Ana oth In 36(2005), 8 vom: 01. Juni, Seite 637-639 volume:36 year:2005 number:8 day:01 month:06 pages:637-639 extent:3 https://doi.org/10.1515/CCLM.1998.112 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 36 2005 8 01 06 637-639 3 |
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Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR |
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Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix, the patient specific T-cell receptor γ rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor γ gene rearrangement. Three patients exhibited two rearranged T-cell receptor γ genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy. Copyright © 1999 by Walter de Gruyter GmbH & Co. KG |
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Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix, the patient specific T-cell receptor γ rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor γ gene rearrangement. Three patients exhibited two rearranged T-cell receptor γ genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy. Copyright © 1999 by Walter de Gruyter GmbH & Co. KG |
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Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix, the patient specific T-cell receptor γ rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor γ gene rearrangement. Three patients exhibited two rearranged T-cell receptor γ genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy. Copyright © 1999 by Walter de Gruyter GmbH & Co. KG |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ246698519</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230505204258.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">220814s2005 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1515/CCLM.1998.112</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">artikel_Grundlieferung.pp</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ246698519</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Analysis of Clonality in T-Lymphoproliferative Diseases by Multiplex PCR</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="b">Walter de Gruyter</subfield><subfield code="c">2005</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">3</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Copyright © 1999 by Walter de Gruyter GmbH & Co. KG</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix, the patient specific T-cell receptor γ rearrangement was determined: five out of ten patients showed the exclusive presence of a single T-cell receptor γ gene rearrangement. Three patients exhibited two rearranged T-cell receptor γ genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segments. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Walter de Gruyter Online Zeitschriften</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zadro, Renata</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sučić, Mirna</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Aurer, Igor</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Metelko-Kovačević, Jasminka</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Labar, Boris</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Stavljenić Rukavina, Ana</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="g">36(2005), 8 vom: 01. Juni, Seite 637-639</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:36</subfield><subfield code="g">year:2005</subfield><subfield code="g">number:8</subfield><subfield code="g">day:01</subfield><subfield code="g">month:06</subfield><subfield code="g">pages:637-639</subfield><subfield code="g">extent:3</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1515/CCLM.1998.112</subfield><subfield code="z">Deutschlandweit zugänglich</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DGR</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">36</subfield><subfield code="j">2005</subfield><subfield code="e">8</subfield><subfield code="b">01</subfield><subfield code="c">06</subfield><subfield code="h">637-639</subfield><subfield code="g">3</subfield></datafield></record></collection>
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