Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche)
Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 ana...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ajubi, Nasser E. [verfasserIn] Nijholt, Nine [verfasserIn] Wolthuis, Albert [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Erschienen: |
Walter de Gruyter ; 2005 |
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| Schlagwörter: |
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| Anmerkung: |
©2005 by Walter de Gruyter Berlin New York |
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| Umfang: |
3 |
| Reproduktion: |
Walter de Gruyter Online Zeitschriften |
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| Übergeordnetes Werk: |
Enthalten in: Clinical chemistry and laboratory medicine - Berlin [u.a.] : De Gruyter, 1998, 43, 1, Seite 68-70 |
| Übergeordnetes Werk: |
volume:43 ; number:1 ; pages:68-70 ; extent:3 |
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| DOI / URN: |
10.1515/CCLM.2005.010 |
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NLEJ246713615 |
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| 520 | |a Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 analyzer to replace the manual qualitative pregnancy test in urine. At urine hCG concentrations of 476, 45 and 11 U/L, we found inter-assay variation of 4.3%, 4.3% and 6.8% and average intra-assay variation of 3.0%, 2.6% and 3.0%, respectively. The analytical detection limit was 0.7 U/L. We did not detect any loss (due to degradation or adsorption) during a storage period of 5 days at 4°C or at −20°C. Recoveries of hCG in urine of a pregnant woman diluted with urine of a pre-menopausal non-pregnant woman (concentration range between 6 and 800 mU/L) were between 93% and 112% (y=0.997x−3.843, r 2=0.999). Diluting a serum sample (hCG 42,000 U/L) with urine (negative for hCG) up to 8000-fold yielded a completely linear hCG response, indicating that the assay was not affected by the urine matrix. In a correlation study with 60 urine samples (of which 10 were of male origin), we did not find any discrepancies between results for the manual pregnancy test and the hCG test on the Roche Modular E170 (using a cutoff value of 50 U/L). | ||
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10.1515/CCLM.2005.010 doi artikel_Grundlieferung.pp (DE-627)NLEJ246713615 DE-627 ger DE-627 rakwb Ajubi, Nasser E. verfasserin aut Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche) Walter de Gruyter 2005 3 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2005 by Walter de Gruyter Berlin New York Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 analyzer to replace the manual qualitative pregnancy test in urine. At urine hCG concentrations of 476, 45 and 11 U/L, we found inter-assay variation of 4.3%, 4.3% and 6.8% and average intra-assay variation of 3.0%, 2.6% and 3.0%, respectively. The analytical detection limit was 0.7 U/L. We did not detect any loss (due to degradation or adsorption) during a storage period of 5 days at 4°C or at −20°C. Recoveries of hCG in urine of a pregnant woman diluted with urine of a pre-menopausal non-pregnant woman (concentration range between 6 and 800 mU/L) were between 93% and 112% (y=0.997x−3.843, r 2=0.999). Diluting a serum sample (hCG 42,000 U/L) with urine (negative for hCG) up to 8000-fold yielded a completely linear hCG response, indicating that the assay was not affected by the urine matrix. In a correlation study with 60 urine samples (of which 10 were of male origin), we did not find any discrepancies between results for the manual pregnancy test and the hCG test on the Roche Modular E170 (using a cutoff value of 50 U/L). Walter de Gruyter Online Zeitschriften cost effectiveness human chorionic gonadotropin (hCG) logistics pregnancy tests urinalysis Nijholt, Nine verfasserin aut Wolthuis, Albert verfasserin aut Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 43, 1, Seite 68-70 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:43 number:1 pages:68-70 extent:3 https://doi.org/10.1515/CCLM.2005.010 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 43 1 68-70 3 |
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10.1515/CCLM.2005.010 doi artikel_Grundlieferung.pp (DE-627)NLEJ246713615 DE-627 ger DE-627 rakwb Ajubi, Nasser E. verfasserin aut Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche) Walter de Gruyter 2005 3 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2005 by Walter de Gruyter Berlin New York Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 analyzer to replace the manual qualitative pregnancy test in urine. At urine hCG concentrations of 476, 45 and 11 U/L, we found inter-assay variation of 4.3%, 4.3% and 6.8% and average intra-assay variation of 3.0%, 2.6% and 3.0%, respectively. The analytical detection limit was 0.7 U/L. We did not detect any loss (due to degradation or adsorption) during a storage period of 5 days at 4°C or at −20°C. Recoveries of hCG in urine of a pregnant woman diluted with urine of a pre-menopausal non-pregnant woman (concentration range between 6 and 800 mU/L) were between 93% and 112% (y=0.997x−3.843, r 2=0.999). Diluting a serum sample (hCG 42,000 U/L) with urine (negative for hCG) up to 8000-fold yielded a completely linear hCG response, indicating that the assay was not affected by the urine matrix. In a correlation study with 60 urine samples (of which 10 were of male origin), we did not find any discrepancies between results for the manual pregnancy test and the hCG test on the Roche Modular E170 (using a cutoff value of 50 U/L). Walter de Gruyter Online Zeitschriften cost effectiveness human chorionic gonadotropin (hCG) logistics pregnancy tests urinalysis Nijholt, Nine verfasserin aut Wolthuis, Albert verfasserin aut Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 43, 1, Seite 68-70 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:43 number:1 pages:68-70 extent:3 https://doi.org/10.1515/CCLM.2005.010 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 43 1 68-70 3 |
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10.1515/CCLM.2005.010 doi artikel_Grundlieferung.pp (DE-627)NLEJ246713615 DE-627 ger DE-627 rakwb Ajubi, Nasser E. verfasserin aut Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche) Walter de Gruyter 2005 3 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2005 by Walter de Gruyter Berlin New York Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 analyzer to replace the manual qualitative pregnancy test in urine. At urine hCG concentrations of 476, 45 and 11 U/L, we found inter-assay variation of 4.3%, 4.3% and 6.8% and average intra-assay variation of 3.0%, 2.6% and 3.0%, respectively. The analytical detection limit was 0.7 U/L. We did not detect any loss (due to degradation or adsorption) during a storage period of 5 days at 4°C or at −20°C. Recoveries of hCG in urine of a pregnant woman diluted with urine of a pre-menopausal non-pregnant woman (concentration range between 6 and 800 mU/L) were between 93% and 112% (y=0.997x−3.843, r 2=0.999). Diluting a serum sample (hCG 42,000 U/L) with urine (negative for hCG) up to 8000-fold yielded a completely linear hCG response, indicating that the assay was not affected by the urine matrix. In a correlation study with 60 urine samples (of which 10 were of male origin), we did not find any discrepancies between results for the manual pregnancy test and the hCG test on the Roche Modular E170 (using a cutoff value of 50 U/L). Walter de Gruyter Online Zeitschriften cost effectiveness human chorionic gonadotropin (hCG) logistics pregnancy tests urinalysis Nijholt, Nine verfasserin aut Wolthuis, Albert verfasserin aut Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 43, 1, Seite 68-70 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:43 number:1 pages:68-70 extent:3 https://doi.org/10.1515/CCLM.2005.010 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 43 1 68-70 3 |
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10.1515/CCLM.2005.010 doi artikel_Grundlieferung.pp (DE-627)NLEJ246713615 DE-627 ger DE-627 rakwb Ajubi, Nasser E. verfasserin aut Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche) Walter de Gruyter 2005 3 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2005 by Walter de Gruyter Berlin New York Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 analyzer to replace the manual qualitative pregnancy test in urine. At urine hCG concentrations of 476, 45 and 11 U/L, we found inter-assay variation of 4.3%, 4.3% and 6.8% and average intra-assay variation of 3.0%, 2.6% and 3.0%, respectively. The analytical detection limit was 0.7 U/L. We did not detect any loss (due to degradation or adsorption) during a storage period of 5 days at 4°C or at −20°C. Recoveries of hCG in urine of a pregnant woman diluted with urine of a pre-menopausal non-pregnant woman (concentration range between 6 and 800 mU/L) were between 93% and 112% (y=0.997x−3.843, r 2=0.999). Diluting a serum sample (hCG 42,000 U/L) with urine (negative for hCG) up to 8000-fold yielded a completely linear hCG response, indicating that the assay was not affected by the urine matrix. In a correlation study with 60 urine samples (of which 10 were of male origin), we did not find any discrepancies between results for the manual pregnancy test and the hCG test on the Roche Modular E170 (using a cutoff value of 50 U/L). Walter de Gruyter Online Zeitschriften cost effectiveness human chorionic gonadotropin (hCG) logistics pregnancy tests urinalysis Nijholt, Nine verfasserin aut Wolthuis, Albert verfasserin aut Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 43, 1, Seite 68-70 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:43 number:1 pages:68-70 extent:3 https://doi.org/10.1515/CCLM.2005.010 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 43 1 68-70 3 |
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Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche) |
| abstract |
Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 analyzer to replace the manual qualitative pregnancy test in urine. At urine hCG concentrations of 476, 45 and 11 U/L, we found inter-assay variation of 4.3%, 4.3% and 6.8% and average intra-assay variation of 3.0%, 2.6% and 3.0%, respectively. The analytical detection limit was 0.7 U/L. We did not detect any loss (due to degradation or adsorption) during a storage period of 5 days at 4°C or at −20°C. Recoveries of hCG in urine of a pregnant woman diluted with urine of a pre-menopausal non-pregnant woman (concentration range between 6 and 800 mU/L) were between 93% and 112% (y=0.997x−3.843, r 2=0.999). Diluting a serum sample (hCG 42,000 U/L) with urine (negative for hCG) up to 8000-fold yielded a completely linear hCG response, indicating that the assay was not affected by the urine matrix. In a correlation study with 60 urine samples (of which 10 were of male origin), we did not find any discrepancies between results for the manual pregnancy test and the hCG test on the Roche Modular E170 (using a cutoff value of 50 U/L). ©2005 by Walter de Gruyter Berlin New York |
| abstractGer |
Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 analyzer to replace the manual qualitative pregnancy test in urine. At urine hCG concentrations of 476, 45 and 11 U/L, we found inter-assay variation of 4.3%, 4.3% and 6.8% and average intra-assay variation of 3.0%, 2.6% and 3.0%, respectively. The analytical detection limit was 0.7 U/L. We did not detect any loss (due to degradation or adsorption) during a storage period of 5 days at 4°C or at −20°C. Recoveries of hCG in urine of a pregnant woman diluted with urine of a pre-menopausal non-pregnant woman (concentration range between 6 and 800 mU/L) were between 93% and 112% (y=0.997x−3.843, r 2=0.999). Diluting a serum sample (hCG 42,000 U/L) with urine (negative for hCG) up to 8000-fold yielded a completely linear hCG response, indicating that the assay was not affected by the urine matrix. In a correlation study with 60 urine samples (of which 10 were of male origin), we did not find any discrepancies between results for the manual pregnancy test and the hCG test on the Roche Modular E170 (using a cutoff value of 50 U/L). ©2005 by Walter de Gruyter Berlin New York |
| abstract_unstemmed |
Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 analyzer to replace the manual qualitative pregnancy test in urine. At urine hCG concentrations of 476, 45 and 11 U/L, we found inter-assay variation of 4.3%, 4.3% and 6.8% and average intra-assay variation of 3.0%, 2.6% and 3.0%, respectively. The analytical detection limit was 0.7 U/L. We did not detect any loss (due to degradation or adsorption) during a storage period of 5 days at 4°C or at −20°C. Recoveries of hCG in urine of a pregnant woman diluted with urine of a pre-menopausal non-pregnant woman (concentration range between 6 and 800 mU/L) were between 93% and 112% (y=0.997x−3.843, r 2=0.999). Diluting a serum sample (hCG 42,000 U/L) with urine (negative for hCG) up to 8000-fold yielded a completely linear hCG response, indicating that the assay was not affected by the urine matrix. In a correlation study with 60 urine samples (of which 10 were of male origin), we did not find any discrepancies between results for the manual pregnancy test and the hCG test on the Roche Modular E170 (using a cutoff value of 50 U/L). ©2005 by Walter de Gruyter Berlin New York |
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Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche) |
| url |
https://doi.org/10.1515/CCLM.2005.010 |
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| author2 |
Nijholt, Nine Wolthuis, Albert |
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| doi_str |
10.1515/CCLM.2005.010 |
| up_date |
2024-07-06T09:09:09.375Z |
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