Analysis of protein S-100B in serum: a methodological study
Background: Dysfunction and damage of the human central nervous system can be detected with biochemical markers, and protein S-100B is the best-established such marker. The aim of this study was to evaluate whether the protein is stable during long-term storage, to establish reference values for the...
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Walter de Gruyter ; 2006 |
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©2006 by Walter de Gruyter Berlin New York |
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Walter de Gruyter Online Zeitschriften |
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Enthalten in: Clinical chemistry and laboratory medicine - Berlin [u.a.] : De Gruyter, 1998, 44, 9, Seite 1111-1114 |
| Übergeordnetes Werk: |
volume:44 ; number:9 ; pages:1111-1114 ; extent:4 |
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| DOI / URN: |
10.1515/CCLM.2006.211 |
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NLEJ246718056 |
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| 520 | |a Background: Dysfunction and damage of the human central nervous system can be detected with biochemical markers, and protein S-100B is the best-established such marker. The aim of this study was to evaluate whether the protein is stable during long-term storage, to establish reference values for the new Elecsys® S100 test and to compare this new method with the Liaison® Sangtec® 100 test. Methods: We analysed blood samples from 118 blood donors and 196 patients with subarachnoid haemorrhage or head injury. The long-term stability of S-100B in frozen serum samples was evaluated with repeated analysis in 1997 and 2003 using an immunoradiometric assay. Method comparison between the Liaison® Sangtec® 100 and Elecsys® S100 tests was performed using Bland-Altman difference plots. Results: Serum concentrations increased significantly during long-term storage (mean difference 0.15 μg/L; ±2 SD, 0.55 μg/L). Serum measurements using the Elecsys® S100 method in 118 healthy blood donors showed S-100B levels between 0.02 and 0.08 μg/L (mean 0.05). The 95th percentile was 0.07 μg/L. The Liaison® Sangtec® 100 test usually measured higher concentrations than the Elecsys® S100 method, and the difference between the two methods increased with increasing concentrations. The mean difference between the methods was 0.14 μg/L (±2 SD, 0.39 μg/L). Conclusions: Protein S-100B is not stable during long-term storage and the two analytical methods are not interchangeable. Clin Chem Lab Med 2006;44:1111–4. | ||
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10.1515/CCLM.2006.211 doi artikel_Grundlieferung.pp (DE-627)NLEJ246718056 DE-627 ger DE-627 rakwb Analysis of protein S-100B in serum: a methodological study Walter de Gruyter 2006 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2006 by Walter de Gruyter Berlin New York Background: Dysfunction and damage of the human central nervous system can be detected with biochemical markers, and protein S-100B is the best-established such marker. The aim of this study was to evaluate whether the protein is stable during long-term storage, to establish reference values for the new Elecsys® S100 test and to compare this new method with the Liaison® Sangtec® 100 test. Methods: We analysed blood samples from 118 blood donors and 196 patients with subarachnoid haemorrhage or head injury. The long-term stability of S-100B in frozen serum samples was evaluated with repeated analysis in 1997 and 2003 using an immunoradiometric assay. Method comparison between the Liaison® Sangtec® 100 and Elecsys® S100 tests was performed using Bland-Altman difference plots. Results: Serum concentrations increased significantly during long-term storage (mean difference 0.15 μg/L; ±2 SD, 0.55 μg/L). Serum measurements using the Elecsys® S100 method in 118 healthy blood donors showed S-100B levels between 0.02 and 0.08 μg/L (mean 0.05). The 95th percentile was 0.07 μg/L. The Liaison® Sangtec® 100 test usually measured higher concentrations than the Elecsys® S100 method, and the difference between the two methods increased with increasing concentrations. The mean difference between the methods was 0.14 μg/L (±2 SD, 0.39 μg/L). Conclusions: Protein S-100B is not stable during long-term storage and the two analytical methods are not interchangeable. Clin Chem Lab Med 2006;44:1111–4. Walter de Gruyter Online Zeitschriften methods protein S-100B serum analysis serum markers traumatic brain injury Müller, Kay oth Elverland, Astrid oth Romner, Bertil oth Waterloo, Knut oth Langbakk, Bodil oth Undén, Johan oth Ingebrigtsen, Tor oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 44, 9, Seite 1111-1114 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:44 number:9 pages:1111-1114 extent:4 https://doi.org/10.1515/CCLM.2006.211 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 44 9 1111-1114 4 |
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10.1515/CCLM.2006.211 doi artikel_Grundlieferung.pp (DE-627)NLEJ246718056 DE-627 ger DE-627 rakwb Analysis of protein S-100B in serum: a methodological study Walter de Gruyter 2006 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2006 by Walter de Gruyter Berlin New York Background: Dysfunction and damage of the human central nervous system can be detected with biochemical markers, and protein S-100B is the best-established such marker. The aim of this study was to evaluate whether the protein is stable during long-term storage, to establish reference values for the new Elecsys® S100 test and to compare this new method with the Liaison® Sangtec® 100 test. Methods: We analysed blood samples from 118 blood donors and 196 patients with subarachnoid haemorrhage or head injury. The long-term stability of S-100B in frozen serum samples was evaluated with repeated analysis in 1997 and 2003 using an immunoradiometric assay. Method comparison between the Liaison® Sangtec® 100 and Elecsys® S100 tests was performed using Bland-Altman difference plots. Results: Serum concentrations increased significantly during long-term storage (mean difference 0.15 μg/L; ±2 SD, 0.55 μg/L). Serum measurements using the Elecsys® S100 method in 118 healthy blood donors showed S-100B levels between 0.02 and 0.08 μg/L (mean 0.05). The 95th percentile was 0.07 μg/L. The Liaison® Sangtec® 100 test usually measured higher concentrations than the Elecsys® S100 method, and the difference between the two methods increased with increasing concentrations. The mean difference between the methods was 0.14 μg/L (±2 SD, 0.39 μg/L). Conclusions: Protein S-100B is not stable during long-term storage and the two analytical methods are not interchangeable. Clin Chem Lab Med 2006;44:1111–4. Walter de Gruyter Online Zeitschriften methods protein S-100B serum analysis serum markers traumatic brain injury Müller, Kay oth Elverland, Astrid oth Romner, Bertil oth Waterloo, Knut oth Langbakk, Bodil oth Undén, Johan oth Ingebrigtsen, Tor oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 44, 9, Seite 1111-1114 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:44 number:9 pages:1111-1114 extent:4 https://doi.org/10.1515/CCLM.2006.211 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 44 9 1111-1114 4 |
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10.1515/CCLM.2006.211 doi artikel_Grundlieferung.pp (DE-627)NLEJ246718056 DE-627 ger DE-627 rakwb Analysis of protein S-100B in serum: a methodological study Walter de Gruyter 2006 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2006 by Walter de Gruyter Berlin New York Background: Dysfunction and damage of the human central nervous system can be detected with biochemical markers, and protein S-100B is the best-established such marker. The aim of this study was to evaluate whether the protein is stable during long-term storage, to establish reference values for the new Elecsys® S100 test and to compare this new method with the Liaison® Sangtec® 100 test. Methods: We analysed blood samples from 118 blood donors and 196 patients with subarachnoid haemorrhage or head injury. The long-term stability of S-100B in frozen serum samples was evaluated with repeated analysis in 1997 and 2003 using an immunoradiometric assay. Method comparison between the Liaison® Sangtec® 100 and Elecsys® S100 tests was performed using Bland-Altman difference plots. Results: Serum concentrations increased significantly during long-term storage (mean difference 0.15 μg/L; ±2 SD, 0.55 μg/L). Serum measurements using the Elecsys® S100 method in 118 healthy blood donors showed S-100B levels between 0.02 and 0.08 μg/L (mean 0.05). The 95th percentile was 0.07 μg/L. The Liaison® Sangtec® 100 test usually measured higher concentrations than the Elecsys® S100 method, and the difference between the two methods increased with increasing concentrations. The mean difference between the methods was 0.14 μg/L (±2 SD, 0.39 μg/L). Conclusions: Protein S-100B is not stable during long-term storage and the two analytical methods are not interchangeable. Clin Chem Lab Med 2006;44:1111–4. Walter de Gruyter Online Zeitschriften methods protein S-100B serum analysis serum markers traumatic brain injury Müller, Kay oth Elverland, Astrid oth Romner, Bertil oth Waterloo, Knut oth Langbakk, Bodil oth Undén, Johan oth Ingebrigtsen, Tor oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 44, 9, Seite 1111-1114 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:44 number:9 pages:1111-1114 extent:4 https://doi.org/10.1515/CCLM.2006.211 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 44 9 1111-1114 4 |
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10.1515/CCLM.2006.211 doi artikel_Grundlieferung.pp (DE-627)NLEJ246718056 DE-627 ger DE-627 rakwb Analysis of protein S-100B in serum: a methodological study Walter de Gruyter 2006 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2006 by Walter de Gruyter Berlin New York Background: Dysfunction and damage of the human central nervous system can be detected with biochemical markers, and protein S-100B is the best-established such marker. The aim of this study was to evaluate whether the protein is stable during long-term storage, to establish reference values for the new Elecsys® S100 test and to compare this new method with the Liaison® Sangtec® 100 test. Methods: We analysed blood samples from 118 blood donors and 196 patients with subarachnoid haemorrhage or head injury. The long-term stability of S-100B in frozen serum samples was evaluated with repeated analysis in 1997 and 2003 using an immunoradiometric assay. Method comparison between the Liaison® Sangtec® 100 and Elecsys® S100 tests was performed using Bland-Altman difference plots. Results: Serum concentrations increased significantly during long-term storage (mean difference 0.15 μg/L; ±2 SD, 0.55 μg/L). Serum measurements using the Elecsys® S100 method in 118 healthy blood donors showed S-100B levels between 0.02 and 0.08 μg/L (mean 0.05). The 95th percentile was 0.07 μg/L. The Liaison® Sangtec® 100 test usually measured higher concentrations than the Elecsys® S100 method, and the difference between the two methods increased with increasing concentrations. The mean difference between the methods was 0.14 μg/L (±2 SD, 0.39 μg/L). Conclusions: Protein S-100B is not stable during long-term storage and the two analytical methods are not interchangeable. Clin Chem Lab Med 2006;44:1111–4. Walter de Gruyter Online Zeitschriften methods protein S-100B serum analysis serum markers traumatic brain injury Müller, Kay oth Elverland, Astrid oth Romner, Bertil oth Waterloo, Knut oth Langbakk, Bodil oth Undén, Johan oth Ingebrigtsen, Tor oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 44, 9, Seite 1111-1114 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:44 number:9 pages:1111-1114 extent:4 https://doi.org/10.1515/CCLM.2006.211 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 44 9 1111-1114 4 |
| allfieldsSound |
10.1515/CCLM.2006.211 doi artikel_Grundlieferung.pp (DE-627)NLEJ246718056 DE-627 ger DE-627 rakwb Analysis of protein S-100B in serum: a methodological study Walter de Gruyter 2006 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ©2006 by Walter de Gruyter Berlin New York Background: Dysfunction and damage of the human central nervous system can be detected with biochemical markers, and protein S-100B is the best-established such marker. The aim of this study was to evaluate whether the protein is stable during long-term storage, to establish reference values for the new Elecsys® S100 test and to compare this new method with the Liaison® Sangtec® 100 test. Methods: We analysed blood samples from 118 blood donors and 196 patients with subarachnoid haemorrhage or head injury. The long-term stability of S-100B in frozen serum samples was evaluated with repeated analysis in 1997 and 2003 using an immunoradiometric assay. Method comparison between the Liaison® Sangtec® 100 and Elecsys® S100 tests was performed using Bland-Altman difference plots. Results: Serum concentrations increased significantly during long-term storage (mean difference 0.15 μg/L; ±2 SD, 0.55 μg/L). Serum measurements using the Elecsys® S100 method in 118 healthy blood donors showed S-100B levels between 0.02 and 0.08 μg/L (mean 0.05). The 95th percentile was 0.07 μg/L. The Liaison® Sangtec® 100 test usually measured higher concentrations than the Elecsys® S100 method, and the difference between the two methods increased with increasing concentrations. The mean difference between the methods was 0.14 μg/L (±2 SD, 0.39 μg/L). Conclusions: Protein S-100B is not stable during long-term storage and the two analytical methods are not interchangeable. Clin Chem Lab Med 2006;44:1111–4. Walter de Gruyter Online Zeitschriften methods protein S-100B serum analysis serum markers traumatic brain injury Müller, Kay oth Elverland, Astrid oth Romner, Bertil oth Waterloo, Knut oth Langbakk, Bodil oth Undén, Johan oth Ingebrigtsen, Tor oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 44, 9, Seite 1111-1114 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:44 number:9 pages:1111-1114 extent:4 https://doi.org/10.1515/CCLM.2006.211 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 44 9 1111-1114 4 |
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Analysis of protein S-100B in serum: a methodological study |
| abstract |
Background: Dysfunction and damage of the human central nervous system can be detected with biochemical markers, and protein S-100B is the best-established such marker. The aim of this study was to evaluate whether the protein is stable during long-term storage, to establish reference values for the new Elecsys® S100 test and to compare this new method with the Liaison® Sangtec® 100 test. Methods: We analysed blood samples from 118 blood donors and 196 patients with subarachnoid haemorrhage or head injury. The long-term stability of S-100B in frozen serum samples was evaluated with repeated analysis in 1997 and 2003 using an immunoradiometric assay. Method comparison between the Liaison® Sangtec® 100 and Elecsys® S100 tests was performed using Bland-Altman difference plots. Results: Serum concentrations increased significantly during long-term storage (mean difference 0.15 μg/L; ±2 SD, 0.55 μg/L). Serum measurements using the Elecsys® S100 method in 118 healthy blood donors showed S-100B levels between 0.02 and 0.08 μg/L (mean 0.05). The 95th percentile was 0.07 μg/L. The Liaison® Sangtec® 100 test usually measured higher concentrations than the Elecsys® S100 method, and the difference between the two methods increased with increasing concentrations. The mean difference between the methods was 0.14 μg/L (±2 SD, 0.39 μg/L). Conclusions: Protein S-100B is not stable during long-term storage and the two analytical methods are not interchangeable. Clin Chem Lab Med 2006;44:1111–4. ©2006 by Walter de Gruyter Berlin New York |
| abstractGer |
Background: Dysfunction and damage of the human central nervous system can be detected with biochemical markers, and protein S-100B is the best-established such marker. The aim of this study was to evaluate whether the protein is stable during long-term storage, to establish reference values for the new Elecsys® S100 test and to compare this new method with the Liaison® Sangtec® 100 test. Methods: We analysed blood samples from 118 blood donors and 196 patients with subarachnoid haemorrhage or head injury. The long-term stability of S-100B in frozen serum samples was evaluated with repeated analysis in 1997 and 2003 using an immunoradiometric assay. Method comparison between the Liaison® Sangtec® 100 and Elecsys® S100 tests was performed using Bland-Altman difference plots. Results: Serum concentrations increased significantly during long-term storage (mean difference 0.15 μg/L; ±2 SD, 0.55 μg/L). Serum measurements using the Elecsys® S100 method in 118 healthy blood donors showed S-100B levels between 0.02 and 0.08 μg/L (mean 0.05). The 95th percentile was 0.07 μg/L. The Liaison® Sangtec® 100 test usually measured higher concentrations than the Elecsys® S100 method, and the difference between the two methods increased with increasing concentrations. The mean difference between the methods was 0.14 μg/L (±2 SD, 0.39 μg/L). Conclusions: Protein S-100B is not stable during long-term storage and the two analytical methods are not interchangeable. Clin Chem Lab Med 2006;44:1111–4. ©2006 by Walter de Gruyter Berlin New York |
| abstract_unstemmed |
Background: Dysfunction and damage of the human central nervous system can be detected with biochemical markers, and protein S-100B is the best-established such marker. The aim of this study was to evaluate whether the protein is stable during long-term storage, to establish reference values for the new Elecsys® S100 test and to compare this new method with the Liaison® Sangtec® 100 test. Methods: We analysed blood samples from 118 blood donors and 196 patients with subarachnoid haemorrhage or head injury. The long-term stability of S-100B in frozen serum samples was evaluated with repeated analysis in 1997 and 2003 using an immunoradiometric assay. Method comparison between the Liaison® Sangtec® 100 and Elecsys® S100 tests was performed using Bland-Altman difference plots. Results: Serum concentrations increased significantly during long-term storage (mean difference 0.15 μg/L; ±2 SD, 0.55 μg/L). Serum measurements using the Elecsys® S100 method in 118 healthy blood donors showed S-100B levels between 0.02 and 0.08 μg/L (mean 0.05). The 95th percentile was 0.07 μg/L. The Liaison® Sangtec® 100 test usually measured higher concentrations than the Elecsys® S100 method, and the difference between the two methods increased with increasing concentrations. The mean difference between the methods was 0.14 μg/L (±2 SD, 0.39 μg/L). Conclusions: Protein S-100B is not stable during long-term storage and the two analytical methods are not interchangeable. Clin Chem Lab Med 2006;44:1111–4. ©2006 by Walter de Gruyter Berlin New York |
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| title_short |
Analysis of protein S-100B in serum: a methodological study |
| url |
https://doi.org/10.1515/CCLM.2006.211 |
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Müller, Kay Elverland, Astrid Romner, Bertil Waterloo, Knut Langbakk, Bodil Undén, Johan Ingebrigtsen, Tor |
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Müller, Kay Elverland, Astrid Romner, Bertil Waterloo, Knut Langbakk, Bodil Undén, Johan Ingebrigtsen, Tor |
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10.1515/CCLM.2006.211 |
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2024-07-06T09:10:08.669Z |
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