Performance of the Roche Total Mycophenolic Acid® assay on the Cobas Integra 400®, Cobas 6000® and comparison to LC-MS/MS in liver transplant patients
Background: Mycophenolic acid (MPA) is an immunosuppressant for which therapeutic drug monitoring (TDM) is performed for optimal prophylaxis and avoidance of toxicity in transplant patients. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for TDM of MPA. There have been s...
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Gespeichert in:
| Autor*in: |
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| Format: |
E-Artikel |
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| Erschienen: |
Walter de Gruyter ; 2011 |
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| Schlagwörter: |
inosine monophosphate dehydrogenase |
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| Umfang: |
7 |
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| Reproduktion: |
Walter de Gruyter Online Zeitschriften |
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| Übergeordnetes Werk: |
Enthalten in: Clinical chemistry and laboratory medicine - Berlin [u.a.] : De Gruyter, 1998, 49(2011), 7 vom: 13. Juni, Seite 1159-1165 |
| Übergeordnetes Werk: |
volume:49 ; year:2011 ; number:7 ; day:13 ; month:06 ; pages:1159-1165 ; extent:7 |
| Links: |
|---|
| DOI / URN: |
10.1515/CCLM.2011.194 |
|---|
| Katalog-ID: |
NLEJ246734353 |
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| 520 | |a Background: Mycophenolic acid (MPA) is an immunosuppressant for which therapeutic drug monitoring (TDM) is performed for optimal prophylaxis and avoidance of toxicity in transplant patients. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for TDM of MPA. There have been several method comparisons of the Roche Total MPA assay, but none have been performed with respect to liver transplant patients. Methods: We validated the Roche Total MPA assay on the Cobas Integra 400 and Cobas 6000 and compared it to a validated LC-MS/MS (API 2000™) method. Fifty-five EDTA plasma samples from liver transplant patients were measured with the Roche assay on these platforms and compared to the LC-MS/MS results. Results: Validation of the LC-MS/MS, Cobas Integra 400 and 6000 was performed with good results. The LC-MS/MS/Integra 400/Cobas 6000 were linear up to 30, 15 and 17 mg/L, respectively. Imprecision was <10% for LC-MS/MS and <7% for the Roche assay on both platforms. The samples showed good agreement with LC-MS/MS. Passing-Bablok regression analysis showed Cobas Integra (mg/L)=1.02×LC-MS/MS (mg/L)–0.50 and Cobas 6000 (mg/L)=0.98×LC-MS/MS–0.47. Conclusions: The Roche Total Mycophenolic Acid-assay is suitable for measuring total MPA in plasma from liver transplant patients and is a good alternative for LC-MS/MS. | ||
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10.1515/CCLM.2011.194 doi artikel_Grundlieferung.pp (DE-627)NLEJ246734353 DE-627 ger DE-627 rakwb Performance of the Roche Total Mycophenolic Acid® assay on the Cobas Integra 400®, Cobas 6000® and comparison to LC-MS/MS in liver transplant patients Walter de Gruyter 2011 7 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Mycophenolic acid (MPA) is an immunosuppressant for which therapeutic drug monitoring (TDM) is performed for optimal prophylaxis and avoidance of toxicity in transplant patients. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for TDM of MPA. There have been several method comparisons of the Roche Total MPA assay, but none have been performed with respect to liver transplant patients. Methods: We validated the Roche Total MPA assay on the Cobas Integra 400 and Cobas 6000 and compared it to a validated LC-MS/MS (API 2000™) method. Fifty-five EDTA plasma samples from liver transplant patients were measured with the Roche assay on these platforms and compared to the LC-MS/MS results. Results: Validation of the LC-MS/MS, Cobas Integra 400 and 6000 was performed with good results. The LC-MS/MS/Integra 400/Cobas 6000 were linear up to 30, 15 and 17 mg/L, respectively. Imprecision was <10% for LC-MS/MS and <7% for the Roche assay on both platforms. The samples showed good agreement with LC-MS/MS. Passing-Bablok regression analysis showed Cobas Integra (mg/L)=1.02×LC-MS/MS (mg/L)–0.50 and Cobas 6000 (mg/L)=0.98×LC-MS/MS–0.47. Conclusions: The Roche Total Mycophenolic Acid-assay is suitable for measuring total MPA in plasma from liver transplant patients and is a good alternative for LC-MS/MS. Walter de Gruyter Online Zeitschriften enzyme assay inosine monophosphate dehydrogenase liquid chromatography-tandem mass spectrometry liver transplantation mycophenolic acid Decavele, An-Sofie C. oth Favoreel, Niels oth Heyden, Fien Vander oth Verstraete, Alain G. oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 49(2011), 7 vom: 13. Juni, Seite 1159-1165 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:49 year:2011 number:7 day:13 month:06 pages:1159-1165 extent:7 https://doi.org/10.1515/CCLM.2011.194 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 49 2011 7 13 06 1159-1165 7 |
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10.1515/CCLM.2011.194 doi artikel_Grundlieferung.pp (DE-627)NLEJ246734353 DE-627 ger DE-627 rakwb Performance of the Roche Total Mycophenolic Acid® assay on the Cobas Integra 400®, Cobas 6000® and comparison to LC-MS/MS in liver transplant patients Walter de Gruyter 2011 7 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Mycophenolic acid (MPA) is an immunosuppressant for which therapeutic drug monitoring (TDM) is performed for optimal prophylaxis and avoidance of toxicity in transplant patients. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for TDM of MPA. There have been several method comparisons of the Roche Total MPA assay, but none have been performed with respect to liver transplant patients. Methods: We validated the Roche Total MPA assay on the Cobas Integra 400 and Cobas 6000 and compared it to a validated LC-MS/MS (API 2000™) method. Fifty-five EDTA plasma samples from liver transplant patients were measured with the Roche assay on these platforms and compared to the LC-MS/MS results. Results: Validation of the LC-MS/MS, Cobas Integra 400 and 6000 was performed with good results. The LC-MS/MS/Integra 400/Cobas 6000 were linear up to 30, 15 and 17 mg/L, respectively. Imprecision was <10% for LC-MS/MS and <7% for the Roche assay on both platforms. The samples showed good agreement with LC-MS/MS. Passing-Bablok regression analysis showed Cobas Integra (mg/L)=1.02×LC-MS/MS (mg/L)–0.50 and Cobas 6000 (mg/L)=0.98×LC-MS/MS–0.47. Conclusions: The Roche Total Mycophenolic Acid-assay is suitable for measuring total MPA in plasma from liver transplant patients and is a good alternative for LC-MS/MS. Walter de Gruyter Online Zeitschriften enzyme assay inosine monophosphate dehydrogenase liquid chromatography-tandem mass spectrometry liver transplantation mycophenolic acid Decavele, An-Sofie C. oth Favoreel, Niels oth Heyden, Fien Vander oth Verstraete, Alain G. oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 49(2011), 7 vom: 13. Juni, Seite 1159-1165 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:49 year:2011 number:7 day:13 month:06 pages:1159-1165 extent:7 https://doi.org/10.1515/CCLM.2011.194 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 49 2011 7 13 06 1159-1165 7 |
| allfields_unstemmed |
10.1515/CCLM.2011.194 doi artikel_Grundlieferung.pp (DE-627)NLEJ246734353 DE-627 ger DE-627 rakwb Performance of the Roche Total Mycophenolic Acid® assay on the Cobas Integra 400®, Cobas 6000® and comparison to LC-MS/MS in liver transplant patients Walter de Gruyter 2011 7 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Mycophenolic acid (MPA) is an immunosuppressant for which therapeutic drug monitoring (TDM) is performed for optimal prophylaxis and avoidance of toxicity in transplant patients. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for TDM of MPA. There have been several method comparisons of the Roche Total MPA assay, but none have been performed with respect to liver transplant patients. Methods: We validated the Roche Total MPA assay on the Cobas Integra 400 and Cobas 6000 and compared it to a validated LC-MS/MS (API 2000™) method. Fifty-five EDTA plasma samples from liver transplant patients were measured with the Roche assay on these platforms and compared to the LC-MS/MS results. Results: Validation of the LC-MS/MS, Cobas Integra 400 and 6000 was performed with good results. The LC-MS/MS/Integra 400/Cobas 6000 were linear up to 30, 15 and 17 mg/L, respectively. Imprecision was <10% for LC-MS/MS and <7% for the Roche assay on both platforms. The samples showed good agreement with LC-MS/MS. Passing-Bablok regression analysis showed Cobas Integra (mg/L)=1.02×LC-MS/MS (mg/L)–0.50 and Cobas 6000 (mg/L)=0.98×LC-MS/MS–0.47. Conclusions: The Roche Total Mycophenolic Acid-assay is suitable for measuring total MPA in plasma from liver transplant patients and is a good alternative for LC-MS/MS. Walter de Gruyter Online Zeitschriften enzyme assay inosine monophosphate dehydrogenase liquid chromatography-tandem mass spectrometry liver transplantation mycophenolic acid Decavele, An-Sofie C. oth Favoreel, Niels oth Heyden, Fien Vander oth Verstraete, Alain G. oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 49(2011), 7 vom: 13. Juni, Seite 1159-1165 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:49 year:2011 number:7 day:13 month:06 pages:1159-1165 extent:7 https://doi.org/10.1515/CCLM.2011.194 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 49 2011 7 13 06 1159-1165 7 |
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10.1515/CCLM.2011.194 doi artikel_Grundlieferung.pp (DE-627)NLEJ246734353 DE-627 ger DE-627 rakwb Performance of the Roche Total Mycophenolic Acid® assay on the Cobas Integra 400®, Cobas 6000® and comparison to LC-MS/MS in liver transplant patients Walter de Gruyter 2011 7 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Mycophenolic acid (MPA) is an immunosuppressant for which therapeutic drug monitoring (TDM) is performed for optimal prophylaxis and avoidance of toxicity in transplant patients. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for TDM of MPA. There have been several method comparisons of the Roche Total MPA assay, but none have been performed with respect to liver transplant patients. Methods: We validated the Roche Total MPA assay on the Cobas Integra 400 and Cobas 6000 and compared it to a validated LC-MS/MS (API 2000™) method. Fifty-five EDTA plasma samples from liver transplant patients were measured with the Roche assay on these platforms and compared to the LC-MS/MS results. Results: Validation of the LC-MS/MS, Cobas Integra 400 and 6000 was performed with good results. The LC-MS/MS/Integra 400/Cobas 6000 were linear up to 30, 15 and 17 mg/L, respectively. Imprecision was <10% for LC-MS/MS and <7% for the Roche assay on both platforms. The samples showed good agreement with LC-MS/MS. Passing-Bablok regression analysis showed Cobas Integra (mg/L)=1.02×LC-MS/MS (mg/L)–0.50 and Cobas 6000 (mg/L)=0.98×LC-MS/MS–0.47. Conclusions: The Roche Total Mycophenolic Acid-assay is suitable for measuring total MPA in plasma from liver transplant patients and is a good alternative for LC-MS/MS. Walter de Gruyter Online Zeitschriften enzyme assay inosine monophosphate dehydrogenase liquid chromatography-tandem mass spectrometry liver transplantation mycophenolic acid Decavele, An-Sofie C. oth Favoreel, Niels oth Heyden, Fien Vander oth Verstraete, Alain G. oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 49(2011), 7 vom: 13. Juni, Seite 1159-1165 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:49 year:2011 number:7 day:13 month:06 pages:1159-1165 extent:7 https://doi.org/10.1515/CCLM.2011.194 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 49 2011 7 13 06 1159-1165 7 |
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10.1515/CCLM.2011.194 doi artikel_Grundlieferung.pp (DE-627)NLEJ246734353 DE-627 ger DE-627 rakwb Performance of the Roche Total Mycophenolic Acid® assay on the Cobas Integra 400®, Cobas 6000® and comparison to LC-MS/MS in liver transplant patients Walter de Gruyter 2011 7 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Mycophenolic acid (MPA) is an immunosuppressant for which therapeutic drug monitoring (TDM) is performed for optimal prophylaxis and avoidance of toxicity in transplant patients. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for TDM of MPA. There have been several method comparisons of the Roche Total MPA assay, but none have been performed with respect to liver transplant patients. Methods: We validated the Roche Total MPA assay on the Cobas Integra 400 and Cobas 6000 and compared it to a validated LC-MS/MS (API 2000™) method. Fifty-five EDTA plasma samples from liver transplant patients were measured with the Roche assay on these platforms and compared to the LC-MS/MS results. Results: Validation of the LC-MS/MS, Cobas Integra 400 and 6000 was performed with good results. The LC-MS/MS/Integra 400/Cobas 6000 were linear up to 30, 15 and 17 mg/L, respectively. Imprecision was <10% for LC-MS/MS and <7% for the Roche assay on both platforms. The samples showed good agreement with LC-MS/MS. Passing-Bablok regression analysis showed Cobas Integra (mg/L)=1.02×LC-MS/MS (mg/L)–0.50 and Cobas 6000 (mg/L)=0.98×LC-MS/MS–0.47. Conclusions: The Roche Total Mycophenolic Acid-assay is suitable for measuring total MPA in plasma from liver transplant patients and is a good alternative for LC-MS/MS. Walter de Gruyter Online Zeitschriften enzyme assay inosine monophosphate dehydrogenase liquid chromatography-tandem mass spectrometry liver transplantation mycophenolic acid Decavele, An-Sofie C. oth Favoreel, Niels oth Heyden, Fien Vander oth Verstraete, Alain G. oth Enthalten in Clinical chemistry and laboratory medicine Berlin [u.a.] : De Gruyter, 1998 49(2011), 7 vom: 13. Juni, Seite 1159-1165 (DE-627)NLEJ248235222 (DE-600)1492732-9 1437-4331 nnns volume:49 year:2011 number:7 day:13 month:06 pages:1159-1165 extent:7 https://doi.org/10.1515/CCLM.2011.194 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 49 2011 7 13 06 1159-1165 7 |
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Performance of the Roche Total Mycophenolic Acid® assay on the Cobas Integra 400®, Cobas 6000® and comparison to LC-MS/MS in liver transplant patients |
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Clinical chemistry and laboratory medicine |
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Clinical chemistry and laboratory medicine |
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2011 |
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10.1515/CCLM.2011.194 |
| title_sort |
performance of the roche total mycophenolic acid® assay on the cobas integra 400®, cobas 6000® and comparison to lc-ms/ms in liver transplant patients |
| title_auth |
Performance of the Roche Total Mycophenolic Acid® assay on the Cobas Integra 400®, Cobas 6000® and comparison to LC-MS/MS in liver transplant patients |
| abstract |
Background: Mycophenolic acid (MPA) is an immunosuppressant for which therapeutic drug monitoring (TDM) is performed for optimal prophylaxis and avoidance of toxicity in transplant patients. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for TDM of MPA. There have been several method comparisons of the Roche Total MPA assay, but none have been performed with respect to liver transplant patients. Methods: We validated the Roche Total MPA assay on the Cobas Integra 400 and Cobas 6000 and compared it to a validated LC-MS/MS (API 2000™) method. Fifty-five EDTA plasma samples from liver transplant patients were measured with the Roche assay on these platforms and compared to the LC-MS/MS results. Results: Validation of the LC-MS/MS, Cobas Integra 400 and 6000 was performed with good results. The LC-MS/MS/Integra 400/Cobas 6000 were linear up to 30, 15 and 17 mg/L, respectively. Imprecision was <10% for LC-MS/MS and <7% for the Roche assay on both platforms. The samples showed good agreement with LC-MS/MS. Passing-Bablok regression analysis showed Cobas Integra (mg/L)=1.02×LC-MS/MS (mg/L)–0.50 and Cobas 6000 (mg/L)=0.98×LC-MS/MS–0.47. Conclusions: The Roche Total Mycophenolic Acid-assay is suitable for measuring total MPA in plasma from liver transplant patients and is a good alternative for LC-MS/MS. |
| abstractGer |
Background: Mycophenolic acid (MPA) is an immunosuppressant for which therapeutic drug monitoring (TDM) is performed for optimal prophylaxis and avoidance of toxicity in transplant patients. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for TDM of MPA. There have been several method comparisons of the Roche Total MPA assay, but none have been performed with respect to liver transplant patients. Methods: We validated the Roche Total MPA assay on the Cobas Integra 400 and Cobas 6000 and compared it to a validated LC-MS/MS (API 2000™) method. Fifty-five EDTA plasma samples from liver transplant patients were measured with the Roche assay on these platforms and compared to the LC-MS/MS results. Results: Validation of the LC-MS/MS, Cobas Integra 400 and 6000 was performed with good results. The LC-MS/MS/Integra 400/Cobas 6000 were linear up to 30, 15 and 17 mg/L, respectively. Imprecision was <10% for LC-MS/MS and <7% for the Roche assay on both platforms. The samples showed good agreement with LC-MS/MS. Passing-Bablok regression analysis showed Cobas Integra (mg/L)=1.02×LC-MS/MS (mg/L)–0.50 and Cobas 6000 (mg/L)=0.98×LC-MS/MS–0.47. Conclusions: The Roche Total Mycophenolic Acid-assay is suitable for measuring total MPA in plasma from liver transplant patients and is a good alternative for LC-MS/MS. |
| abstract_unstemmed |
Background: Mycophenolic acid (MPA) is an immunosuppressant for which therapeutic drug monitoring (TDM) is performed for optimal prophylaxis and avoidance of toxicity in transplant patients. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for TDM of MPA. There have been several method comparisons of the Roche Total MPA assay, but none have been performed with respect to liver transplant patients. Methods: We validated the Roche Total MPA assay on the Cobas Integra 400 and Cobas 6000 and compared it to a validated LC-MS/MS (API 2000™) method. Fifty-five EDTA plasma samples from liver transplant patients were measured with the Roche assay on these platforms and compared to the LC-MS/MS results. Results: Validation of the LC-MS/MS, Cobas Integra 400 and 6000 was performed with good results. The LC-MS/MS/Integra 400/Cobas 6000 were linear up to 30, 15 and 17 mg/L, respectively. Imprecision was <10% for LC-MS/MS and <7% for the Roche assay on both platforms. The samples showed good agreement with LC-MS/MS. Passing-Bablok regression analysis showed Cobas Integra (mg/L)=1.02×LC-MS/MS (mg/L)–0.50 and Cobas 6000 (mg/L)=0.98×LC-MS/MS–0.47. Conclusions: The Roche Total Mycophenolic Acid-assay is suitable for measuring total MPA in plasma from liver transplant patients and is a good alternative for LC-MS/MS. |
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| title_short |
Performance of the Roche Total Mycophenolic Acid® assay on the Cobas Integra 400®, Cobas 6000® and comparison to LC-MS/MS in liver transplant patients |
| url |
https://doi.org/10.1515/CCLM.2011.194 |
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Decavele, An-Sofie C. Favoreel, Niels Heyden, Fien Vander Verstraete, Alain G. |
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Decavele, An-Sofie C. Favoreel, Niels Heyden, Fien Vander Verstraete, Alain G. |
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2024-07-06T09:13:38.733Z |
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