Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells
Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hM...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|
| Erschienen: |
De Gruyter ; 2012 |
|---|
| Schlagwörter: |
|---|
| Umfang: |
15 |
|---|
| Reproduktion: |
Walter de Gruyter Online Zeitschriften |
|---|---|
| Übergeordnetes Werk: |
Enthalten in: Biological chemistry - Berlin [u.a.] : de Gruyter, 1996, 393(2012), 12 vom: 13. Nov., Seite 1433-1447 |
| Übergeordnetes Werk: |
volume:393 ; year:2012 ; number:12 ; day:13 ; month:11 ; pages:1433-1447 ; extent:15 |
| Links: |
|---|
| DOI / URN: |
10.1515/hsz-2012-0186 |
|---|
| Katalog-ID: |
NLEJ246932066 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ246932066 | ||
| 003 | DE-627 | ||
| 005 | 20230506180012.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220814s2012 xx |||||o 00| ||und c | ||
| 024 | 7 | |a 10.1515/hsz-2012-0186 |2 doi | |
| 028 | 5 | 2 | |a artikel_Grundlieferung.pp |
| 035 | |a (DE-627)NLEJ246932066 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 245 | 1 | 0 | |a Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells |
| 264 | 1 | |b De Gruyter |c 2012 | |
| 300 | |a 15 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7. | ||
| 533 | |f Walter de Gruyter Online Zeitschriften | ||
| 650 | 4 | |a Frizzled receptors (Fzds) | |
| 650 | 4 | |a human mesenchymal stem cells (hMSCs) | |
| 650 | 4 | |a RNA interference | |
| 650 | 4 | |a TCF/LEF reporter gene system | |
| 650 | 4 | |a Wnt/β-catenin signaling | |
| 700 | 1 | |a Kolben, Thomas |4 oth | |
| 700 | 1 | |a Peröbner, Iris |4 oth | |
| 700 | 1 | |a Fernsebner, Katharina |4 oth | |
| 700 | 1 | |a Lechner, Felix |4 oth | |
| 700 | 1 | |a Geissler, Claudia |4 oth | |
| 700 | 1 | |a Ruiz-Heinrich, Lourdes |4 oth | |
| 700 | 1 | |a Capovilla, Simon |4 oth | |
| 700 | 1 | |a Jochum, Marianne |4 oth | |
| 700 | 1 | |a Neth, Peter |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Biological chemistry |d Berlin [u.a.] : de Gruyter, 1996 |g 393(2012), 12 vom: 13. Nov., Seite 1433-1447 |w (DE-627)NLEJ248235095 |w (DE-600)1466062-3 |x 1437-4315 |7 nnns |
| 773 | 1 | 8 | |g volume:393 |g year:2012 |g number:12 |g day:13 |g month:11 |g pages:1433-1447 |g extent:15 |
| 856 | 4 | 0 | |u https://doi.org/10.1515/hsz-2012-0186 |z Deutschlandweit zugänglich |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-DGR | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 393 |j 2012 |e 12 |b 13 |c 11 |h 1433-1447 |g 15 | ||
| matchkey_str |
article:14374315:2012----::iscighipcofizercposnnctnninlnoh |
|---|---|
| hierarchy_sort_str |
2012 |
| publishDate |
2012 |
| allfields |
10.1515/hsz-2012-0186 doi artikel_Grundlieferung.pp (DE-627)NLEJ246932066 DE-627 ger DE-627 rakwb Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells De Gruyter 2012 15 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7. Walter de Gruyter Online Zeitschriften Frizzled receptors (Fzds) human mesenchymal stem cells (hMSCs) RNA interference TCF/LEF reporter gene system Wnt/β-catenin signaling Kolben, Thomas oth Peröbner, Iris oth Fernsebner, Katharina oth Lechner, Felix oth Geissler, Claudia oth Ruiz-Heinrich, Lourdes oth Capovilla, Simon oth Jochum, Marianne oth Neth, Peter oth Enthalten in Biological chemistry Berlin [u.a.] : de Gruyter, 1996 393(2012), 12 vom: 13. Nov., Seite 1433-1447 (DE-627)NLEJ248235095 (DE-600)1466062-3 1437-4315 nnns volume:393 year:2012 number:12 day:13 month:11 pages:1433-1447 extent:15 https://doi.org/10.1515/hsz-2012-0186 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 393 2012 12 13 11 1433-1447 15 |
| spelling |
10.1515/hsz-2012-0186 doi artikel_Grundlieferung.pp (DE-627)NLEJ246932066 DE-627 ger DE-627 rakwb Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells De Gruyter 2012 15 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7. Walter de Gruyter Online Zeitschriften Frizzled receptors (Fzds) human mesenchymal stem cells (hMSCs) RNA interference TCF/LEF reporter gene system Wnt/β-catenin signaling Kolben, Thomas oth Peröbner, Iris oth Fernsebner, Katharina oth Lechner, Felix oth Geissler, Claudia oth Ruiz-Heinrich, Lourdes oth Capovilla, Simon oth Jochum, Marianne oth Neth, Peter oth Enthalten in Biological chemistry Berlin [u.a.] : de Gruyter, 1996 393(2012), 12 vom: 13. Nov., Seite 1433-1447 (DE-627)NLEJ248235095 (DE-600)1466062-3 1437-4315 nnns volume:393 year:2012 number:12 day:13 month:11 pages:1433-1447 extent:15 https://doi.org/10.1515/hsz-2012-0186 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 393 2012 12 13 11 1433-1447 15 |
| allfields_unstemmed |
10.1515/hsz-2012-0186 doi artikel_Grundlieferung.pp (DE-627)NLEJ246932066 DE-627 ger DE-627 rakwb Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells De Gruyter 2012 15 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7. Walter de Gruyter Online Zeitschriften Frizzled receptors (Fzds) human mesenchymal stem cells (hMSCs) RNA interference TCF/LEF reporter gene system Wnt/β-catenin signaling Kolben, Thomas oth Peröbner, Iris oth Fernsebner, Katharina oth Lechner, Felix oth Geissler, Claudia oth Ruiz-Heinrich, Lourdes oth Capovilla, Simon oth Jochum, Marianne oth Neth, Peter oth Enthalten in Biological chemistry Berlin [u.a.] : de Gruyter, 1996 393(2012), 12 vom: 13. Nov., Seite 1433-1447 (DE-627)NLEJ248235095 (DE-600)1466062-3 1437-4315 nnns volume:393 year:2012 number:12 day:13 month:11 pages:1433-1447 extent:15 https://doi.org/10.1515/hsz-2012-0186 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 393 2012 12 13 11 1433-1447 15 |
| allfieldsGer |
10.1515/hsz-2012-0186 doi artikel_Grundlieferung.pp (DE-627)NLEJ246932066 DE-627 ger DE-627 rakwb Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells De Gruyter 2012 15 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7. Walter de Gruyter Online Zeitschriften Frizzled receptors (Fzds) human mesenchymal stem cells (hMSCs) RNA interference TCF/LEF reporter gene system Wnt/β-catenin signaling Kolben, Thomas oth Peröbner, Iris oth Fernsebner, Katharina oth Lechner, Felix oth Geissler, Claudia oth Ruiz-Heinrich, Lourdes oth Capovilla, Simon oth Jochum, Marianne oth Neth, Peter oth Enthalten in Biological chemistry Berlin [u.a.] : de Gruyter, 1996 393(2012), 12 vom: 13. Nov., Seite 1433-1447 (DE-627)NLEJ248235095 (DE-600)1466062-3 1437-4315 nnns volume:393 year:2012 number:12 day:13 month:11 pages:1433-1447 extent:15 https://doi.org/10.1515/hsz-2012-0186 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 393 2012 12 13 11 1433-1447 15 |
| allfieldsSound |
10.1515/hsz-2012-0186 doi artikel_Grundlieferung.pp (DE-627)NLEJ246932066 DE-627 ger DE-627 rakwb Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells De Gruyter 2012 15 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7. Walter de Gruyter Online Zeitschriften Frizzled receptors (Fzds) human mesenchymal stem cells (hMSCs) RNA interference TCF/LEF reporter gene system Wnt/β-catenin signaling Kolben, Thomas oth Peröbner, Iris oth Fernsebner, Katharina oth Lechner, Felix oth Geissler, Claudia oth Ruiz-Heinrich, Lourdes oth Capovilla, Simon oth Jochum, Marianne oth Neth, Peter oth Enthalten in Biological chemistry Berlin [u.a.] : de Gruyter, 1996 393(2012), 12 vom: 13. Nov., Seite 1433-1447 (DE-627)NLEJ248235095 (DE-600)1466062-3 1437-4315 nnns volume:393 year:2012 number:12 day:13 month:11 pages:1433-1447 extent:15 https://doi.org/10.1515/hsz-2012-0186 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 393 2012 12 13 11 1433-1447 15 |
| source |
Enthalten in Biological chemistry 393(2012), 12 vom: 13. Nov., Seite 1433-1447 volume:393 year:2012 number:12 day:13 month:11 pages:1433-1447 extent:15 |
| sourceStr |
Enthalten in Biological chemistry 393(2012), 12 vom: 13. Nov., Seite 1433-1447 volume:393 year:2012 number:12 day:13 month:11 pages:1433-1447 extent:15 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Frizzled receptors (Fzds) human mesenchymal stem cells (hMSCs) RNA interference TCF/LEF reporter gene system Wnt/β-catenin signaling |
| isfreeaccess_bool |
false |
| container_title |
Biological chemistry |
| authorswithroles_txt_mv |
Kolben, Thomas @@oth@@ Peröbner, Iris @@oth@@ Fernsebner, Katharina @@oth@@ Lechner, Felix @@oth@@ Geissler, Claudia @@oth@@ Ruiz-Heinrich, Lourdes @@oth@@ Capovilla, Simon @@oth@@ Jochum, Marianne @@oth@@ Neth, Peter @@oth@@ |
| publishDateDaySort_date |
2012-11-13T00:00:00Z |
| hierarchy_top_id |
NLEJ248235095 |
| id |
NLEJ246932066 |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ246932066</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506180012.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">220814s2012 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1515/hsz-2012-0186</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">artikel_Grundlieferung.pp</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ246932066</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="b">De Gruyter</subfield><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">15</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Walter de Gruyter Online Zeitschriften</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Frizzled receptors (Fzds)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">human mesenchymal stem cells (hMSCs)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNA interference</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">TCF/LEF reporter gene system</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Wnt/β-catenin signaling</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kolben, Thomas</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Peröbner, Iris</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Fernsebner, Katharina</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lechner, Felix</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Geissler, Claudia</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ruiz-Heinrich, Lourdes</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Capovilla, Simon</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jochum, Marianne</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Neth, Peter</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Biological chemistry</subfield><subfield code="d">Berlin [u.a.] : de Gruyter, 1996</subfield><subfield code="g">393(2012), 12 vom: 13. Nov., Seite 1433-1447</subfield><subfield code="w">(DE-627)NLEJ248235095</subfield><subfield code="w">(DE-600)1466062-3</subfield><subfield code="x">1437-4315</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:393</subfield><subfield code="g">year:2012</subfield><subfield code="g">number:12</subfield><subfield code="g">day:13</subfield><subfield code="g">month:11</subfield><subfield code="g">pages:1433-1447</subfield><subfield code="g">extent:15</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1515/hsz-2012-0186</subfield><subfield code="z">Deutschlandweit zugänglich</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DGR</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">393</subfield><subfield code="j">2012</subfield><subfield code="e">12</subfield><subfield code="b">13</subfield><subfield code="c">11</subfield><subfield code="h">1433-1447</subfield><subfield code="g">15</subfield></datafield></record></collection>
|
| series2 |
Walter de Gruyter Online Zeitschriften |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ248235095 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1437-4315 |
| topic_title |
Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells Frizzled receptors (Fzds) human mesenchymal stem cells (hMSCs) RNA interference TCF/LEF reporter gene system Wnt/β-catenin signaling |
| publisher |
De Gruyter |
| publisherStr |
De Gruyter |
| topic |
misc Frizzled receptors (Fzds) misc human mesenchymal stem cells (hMSCs) misc RNA interference misc TCF/LEF reporter gene system misc Wnt/β-catenin signaling |
| spellingShingle |
misc Frizzled receptors (Fzds) misc human mesenchymal stem cells (hMSCs) misc RNA interference misc TCF/LEF reporter gene system misc Wnt/β-catenin signaling Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells |
| topic_unstemmed |
misc Frizzled receptors (Fzds) misc human mesenchymal stem cells (hMSCs) misc RNA interference misc TCF/LEF reporter gene system misc Wnt/β-catenin signaling |
| topic_browse |
misc Frizzled receptors (Fzds) misc human mesenchymal stem cells (hMSCs) misc RNA interference misc TCF/LEF reporter gene system misc Wnt/β-catenin signaling |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| author2_variant |
t k tk i p ip k f kf f l fl c g cg l r h lrh s c sc m j mj p n pn |
| hierarchy_parent_title |
Biological chemistry |
| hierarchy_parent_id |
NLEJ248235095 |
| hierarchy_top_title |
Biological chemistry |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ248235095 (DE-600)1466062-3 |
| title |
Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells |
| ctrlnum |
(DE-627)NLEJ246932066 |
| title_full |
Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells |
| journal |
Biological chemistry |
| journalStr |
Biological chemistry |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
2012 |
| contenttype_str_mv |
txt |
| container_start_page |
1433 |
| container_volume |
393 |
| physical |
15 |
| format_se |
Elektronische Aufsätze |
| doi_str_mv |
10.1515/hsz-2012-0186 |
| title_sort |
dissecting the impact of frizzled receptors in wnt/β-catenin signaling of human mesenchymal stem cells |
| title_auth |
Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells |
| abstract |
Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7. |
| abstractGer |
Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7. |
| abstract_unstemmed |
Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7. |
| collection_details |
GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE |
| container_issue |
12 |
| title_short |
Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells |
| url |
https://doi.org/10.1515/hsz-2012-0186 |
| remote_bool |
true |
| author2 |
Kolben, Thomas Peröbner, Iris Fernsebner, Katharina Lechner, Felix Geissler, Claudia Ruiz-Heinrich, Lourdes Capovilla, Simon Jochum, Marianne Neth, Peter |
| author2Str |
Kolben, Thomas Peröbner, Iris Fernsebner, Katharina Lechner, Felix Geissler, Claudia Ruiz-Heinrich, Lourdes Capovilla, Simon Jochum, Marianne Neth, Peter |
| ppnlink |
NLEJ248235095 |
| mediatype_str_mv |
c |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth oth oth oth oth oth |
| doi_str |
10.1515/hsz-2012-0186 |
| up_date |
2024-07-06T09:37:46.649Z |
| _version_ |
1803821951646433280 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">NLEJ246932066</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230506180012.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">220814s2012 xx |||||o 00| ||und c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1515/hsz-2012-0186</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">artikel_Grundlieferung.pp</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ246932066</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Dissecting the impact of Frizzled receptors in Wnt/β-catenin signaling of human mesenchymal stem cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="b">De Gruyter</subfield><subfield code="c">2012</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">15</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Wnt/β-catenin signaling is of fundamental importance in the regulation of self-renewal, migration/invasion, and differentiation of human mesenchymal stem cells (hMSCs). Because little information is available about the function of Frizzled receptors (Fzds) as the main receptors of Wnt proteins in hMSCs, we first performed comparative Fzd mRNA expression profiling. Fzd9 and Fzd10 were not expressed in hMSCs. While Fzd3 was expressed at low levels in hMSCs, the other Fzds exhibited high expression rates. Activation and repression of Wnt signaling in hMSCs revealed that the expression levels of Fzd1, Fzd6, and Fzd7 are positively correlated with the Wnt/β-catenin activation status, whereas Fzd8 exhibited an inverse relation. For studying the functional relevance of Fzds in Wnt/β-catenin signaling, RNA interference, ectopic expression studies, and rescue approaches were performed in hMSCs carrying a highly sensitive TCF/LEF reporter gene system (Gaussia luciferase). We found that, Fzd1, Fzd5, Fzd7, and Fzd8 are largely involved in Wnt/β-catenin signaling of hMSCs. Moreover, the knockdown of Fzd5 can be compensated by the ectopic expression of Fzd7. Conversely, the ectopic expression of Fzd5 in Fzd7-knockdown hMSCs resulted in a rescue of Wnt/β-catenin signaling, pointing to a functional redundancy of Fzd5 and Fzd7.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">Walter de Gruyter Online Zeitschriften</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Frizzled receptors (Fzds)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">human mesenchymal stem cells (hMSCs)</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNA interference</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">TCF/LEF reporter gene system</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Wnt/β-catenin signaling</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kolben, Thomas</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Peröbner, Iris</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Fernsebner, Katharina</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lechner, Felix</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Geissler, Claudia</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ruiz-Heinrich, Lourdes</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Capovilla, Simon</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jochum, Marianne</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Neth, Peter</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Biological chemistry</subfield><subfield code="d">Berlin [u.a.] : de Gruyter, 1996</subfield><subfield code="g">393(2012), 12 vom: 13. Nov., Seite 1433-1447</subfield><subfield code="w">(DE-627)NLEJ248235095</subfield><subfield code="w">(DE-600)1466062-3</subfield><subfield code="x">1437-4315</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:393</subfield><subfield code="g">year:2012</subfield><subfield code="g">number:12</subfield><subfield code="g">day:13</subfield><subfield code="g">month:11</subfield><subfield code="g">pages:1433-1447</subfield><subfield code="g">extent:15</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1515/hsz-2012-0186</subfield><subfield code="z">Deutschlandweit zugänglich</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-DGR</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">393</subfield><subfield code="j">2012</subfield><subfield code="e">12</subfield><subfield code="b">13</subfield><subfield code="c">11</subfield><subfield code="h">1433-1447</subfield><subfield code="g">15</subfield></datafield></record></collection>
|
| score |
7.4006166 |