Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver
Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell vi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Marin, D.E. [verfasserIn] Pistol, G. C. [verfasserIn] Taranu, I. [verfasserIn] |
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E-Artikel |
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| Erschienen: |
Sciendo ; 2020 |
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| Schlagwörter: |
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| Umfang: |
8 |
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| Reproduktion: |
Walter de Gruyter Online Zeitschriften |
|---|---|
| Übergeordnetes Werk: |
Enthalten in: Archiva Zootechnica - 23(2020), 1 vom: 02. Okt., Seite 73-80 |
| Übergeordnetes Werk: |
volume:23 ; year:2020 ; number:1 ; day:02 ; month:10 ; pages:73-80 ; extent:8 |
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| DOI / URN: |
10.2478/azibna-2020-0007 |
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NLEJ247938572 |
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10.2478/azibna-2020-0007 doi articles2015-2020.pp (DE-627)NLEJ247938572 DE-627 ger DE-627 rakwb Marin, D.E. verfasserin aut Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver Sciendo 2020 8 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. In this context, a re-evaluation of the guidance levels for mycotoxins will be required in the future, in order to reduce the health risk associated with the possible consumption of mycotoxin co-contaminated food or feed. Walter de Gruyter Online Zeitschriften zearalenone ochratoxin liver co-contamination HepG2 Pistol, G. C. verfasserin aut Taranu, I. verfasserin aut Enthalten in Archiva Zootechnica 23(2020), 1 vom: 02. Okt., Seite 73-80 (DE-627)NLEJ248237209 2344-4592 nnns volume:23 year:2020 number:1 day:02 month:10 pages:73-80 extent:8 https://doi.org/10.2478/azibna-2020-0007 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 23 2020 1 02 10 73-80 8 |
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10.2478/azibna-2020-0007 doi articles2015-2020.pp (DE-627)NLEJ247938572 DE-627 ger DE-627 rakwb Marin, D.E. verfasserin aut Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver Sciendo 2020 8 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. In this context, a re-evaluation of the guidance levels for mycotoxins will be required in the future, in order to reduce the health risk associated with the possible consumption of mycotoxin co-contaminated food or feed. Walter de Gruyter Online Zeitschriften zearalenone ochratoxin liver co-contamination HepG2 Pistol, G. C. verfasserin aut Taranu, I. verfasserin aut Enthalten in Archiva Zootechnica 23(2020), 1 vom: 02. Okt., Seite 73-80 (DE-627)NLEJ248237209 2344-4592 nnns volume:23 year:2020 number:1 day:02 month:10 pages:73-80 extent:8 https://doi.org/10.2478/azibna-2020-0007 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 23 2020 1 02 10 73-80 8 |
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10.2478/azibna-2020-0007 doi articles2015-2020.pp (DE-627)NLEJ247938572 DE-627 ger DE-627 rakwb Marin, D.E. verfasserin aut Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver Sciendo 2020 8 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. In this context, a re-evaluation of the guidance levels for mycotoxins will be required in the future, in order to reduce the health risk associated with the possible consumption of mycotoxin co-contaminated food or feed. Walter de Gruyter Online Zeitschriften zearalenone ochratoxin liver co-contamination HepG2 Pistol, G. C. verfasserin aut Taranu, I. verfasserin aut Enthalten in Archiva Zootechnica 23(2020), 1 vom: 02. Okt., Seite 73-80 (DE-627)NLEJ248237209 2344-4592 nnns volume:23 year:2020 number:1 day:02 month:10 pages:73-80 extent:8 https://doi.org/10.2478/azibna-2020-0007 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 23 2020 1 02 10 73-80 8 |
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10.2478/azibna-2020-0007 doi articles2015-2020.pp (DE-627)NLEJ247938572 DE-627 ger DE-627 rakwb Marin, D.E. verfasserin aut Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver Sciendo 2020 8 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. In this context, a re-evaluation of the guidance levels for mycotoxins will be required in the future, in order to reduce the health risk associated with the possible consumption of mycotoxin co-contaminated food or feed. Walter de Gruyter Online Zeitschriften zearalenone ochratoxin liver co-contamination HepG2 Pistol, G. C. verfasserin aut Taranu, I. verfasserin aut Enthalten in Archiva Zootechnica 23(2020), 1 vom: 02. Okt., Seite 73-80 (DE-627)NLEJ248237209 2344-4592 nnns volume:23 year:2020 number:1 day:02 month:10 pages:73-80 extent:8 https://doi.org/10.2478/azibna-2020-0007 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 23 2020 1 02 10 73-80 8 |
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10.2478/azibna-2020-0007 doi articles2015-2020.pp (DE-627)NLEJ247938572 DE-627 ger DE-627 rakwb Marin, D.E. verfasserin aut Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver Sciendo 2020 8 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. In this context, a re-evaluation of the guidance levels for mycotoxins will be required in the future, in order to reduce the health risk associated with the possible consumption of mycotoxin co-contaminated food or feed. Walter de Gruyter Online Zeitschriften zearalenone ochratoxin liver co-contamination HepG2 Pistol, G. C. verfasserin aut Taranu, I. verfasserin aut Enthalten in Archiva Zootechnica 23(2020), 1 vom: 02. Okt., Seite 73-80 (DE-627)NLEJ248237209 2344-4592 nnns volume:23 year:2020 number:1 day:02 month:10 pages:73-80 extent:8 https://doi.org/10.2478/azibna-2020-0007 Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-DGR GBV_NL_ARTICLE AR 23 2020 1 02 10 73-80 8 |
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Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver |
| abstract |
Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. In this context, a re-evaluation of the guidance levels for mycotoxins will be required in the future, in order to reduce the health risk associated with the possible consumption of mycotoxin co-contaminated food or feed. |
| abstractGer |
Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. In this context, a re-evaluation of the guidance levels for mycotoxins will be required in the future, in order to reduce the health risk associated with the possible consumption of mycotoxin co-contaminated food or feed. |
| abstract_unstemmed |
Co-contamination with mycotoxin represents a serious concern for human and animal health. In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. In this context, a re-evaluation of the guidance levels for mycotoxins will be required in the future, in order to reduce the health risk associated with the possible consumption of mycotoxin co-contaminated food or feed. |
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Cytotoxic effects of individual and binary combinations of zearalenone and ochratoxin a on liver |
| url |
https://doi.org/10.2478/azibna-2020-0007 |
| remote_bool |
true |
| author2 |
Pistol, G. C. Taranu, I. |
| author2Str |
Pistol, G. C. Taranu, I. |
| ppnlink |
NLEJ248237209 |
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c |
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false |
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| doi_str |
10.2478/azibna-2020-0007 |
| up_date |
2024-07-05T22:06:57.504Z |
| _version_ |
1803778489064620032 |
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In this in vitro study, we investigated the combined effects of ZEA and OTA, mycotoxins which frequently contaminate cereals, in binary mixtures on the viability of human liver cancer cell line (HepG2). Cell viability was assessed after 24 h using a neutral red assay. An antagonistic effect was observed for binary toxins combinations affecting 25% of cell viability (CI=4.18), which turn into a synergistic effect as followed: slight at IL50 (CI=1.51), moderate at IL75 (CI=0.554) and strong at IL90 (CI=0.203). In conclusion, our results show an important additive and even synergistic cytotoxic effect of two commonly occurred mycotoxins: zearalenone and ochratoxin when they are present simultaneously in food or feed. The co-exposure to mycotoxins lead to a higher toxicity than the exposure to single toxin. Our study provides important data for mycotoxins risk assessment. 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