Role of Molecular Size in Ratchet Fractionation
We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow ga...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
|---|
| Format: |
E-Artikel |
|---|
| Erschienen: |
2002 |
|---|
| Umfang: |
Online-Ressource 4 |
|---|
| Reproduktion: |
APS Digital Backfile Archive 1893-2003 |
|---|---|
| Übergeordnetes Werk: |
Enthalten in: Physical review letters - College Park, Md. : APS, 1958, 89(2002), 17 |
| Übergeordnetes Werk: |
volume:89 ; year:2002 ; number:17 ; extent:4 |
| Links: |
|---|
| Katalog-ID: |
NLEJ250596881 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLEJ250596881 | ||
| 003 | DE-627 | ||
| 005 | 20231114105120.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231114s2002 xx |||||o 00| ||und c | ||
| 035 | |a (DE-627)NLEJ250596881 | ||
| 035 | |a (DE-601)aps:00f17e11d39ba7d72bae0e859dacee53a46d3975 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 245 | 1 | 0 | |a Role of Molecular Size in Ratchet Fractionation |
| 264 | 1 | |c 2002 | |
| 300 | |a Online-Ressource | ||
| 300 | |a 4 | ||
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs. | ||
| 533 | |f APS Digital Backfile Archive 1893-2003 | ||
| 700 | 1 | |a Huang, Lotien Richard |4 oth | |
| 700 | 1 | |a Silberzan, Pascal |4 oth | |
| 700 | 1 | |a Tegenfeldt, Jonas O. |4 oth | |
| 700 | 1 | |a Cox, Edward C. |4 oth | |
| 700 | 1 | |a Sturm, James C. |4 oth | |
| 700 | 1 | |a Austin, <?format <format:justify></format:justify>?>Robert H. |4 oth | |
| 700 | 1 | |a Craighead, Harold |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Physical review letters |d College Park, Md. : APS, 1958 |g 89(2002), 17 |h Online-Ressource |w (DE-627)NLEJ248237888 |w (DE-600)1472655-5 |x 1079-7114 |7 nnns |
| 773 | 1 | 8 | |g volume:89 |g year:2002 |g number:17 |g extent:4 |
| 856 | 4 | 0 | |u https://www.tib.eu/de/suchen/id/aps%3A00f17e11d39ba7d72bae0e859dacee53a46d3975 |x Verlag |z Deutschlandweit zugänglich |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a ZDB-1-APS | ||
| 912 | |a GBV_NL_ARTICLE | ||
| 951 | |a AR | ||
| 952 | |d 89 |j 2002 |e 17 |g 4 | ||
| matchkey_str |
article:10797114:2002----::oefoeuaszirthtr |
|---|---|
| hierarchy_sort_str |
2002 |
| publishDate |
2002 |
| allfields |
(DE-627)NLEJ250596881 (DE-601)aps:00f17e11d39ba7d72bae0e859dacee53a46d3975 DE-627 ger DE-627 rakwb Role of Molecular Size in Ratchet Fractionation 2002 Online-Ressource 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs. APS Digital Backfile Archive 1893-2003 Huang, Lotien Richard oth Silberzan, Pascal oth Tegenfeldt, Jonas O. oth Cox, Edward C. oth Sturm, James C. oth Austin, <?format <format:justify></format:justify>?>Robert H. oth Craighead, Harold oth Enthalten in Physical review letters College Park, Md. : APS, 1958 89(2002), 17 Online-Ressource (DE-627)NLEJ248237888 (DE-600)1472655-5 1079-7114 nnns volume:89 year:2002 number:17 extent:4 https://www.tib.eu/de/suchen/id/aps%3A00f17e11d39ba7d72bae0e859dacee53a46d3975 Verlag Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-APS GBV_NL_ARTICLE AR 89 2002 17 4 |
| spelling |
(DE-627)NLEJ250596881 (DE-601)aps:00f17e11d39ba7d72bae0e859dacee53a46d3975 DE-627 ger DE-627 rakwb Role of Molecular Size in Ratchet Fractionation 2002 Online-Ressource 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs. APS Digital Backfile Archive 1893-2003 Huang, Lotien Richard oth Silberzan, Pascal oth Tegenfeldt, Jonas O. oth Cox, Edward C. oth Sturm, James C. oth Austin, <?format <format:justify></format:justify>?>Robert H. oth Craighead, Harold oth Enthalten in Physical review letters College Park, Md. : APS, 1958 89(2002), 17 Online-Ressource (DE-627)NLEJ248237888 (DE-600)1472655-5 1079-7114 nnns volume:89 year:2002 number:17 extent:4 https://www.tib.eu/de/suchen/id/aps%3A00f17e11d39ba7d72bae0e859dacee53a46d3975 Verlag Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-APS GBV_NL_ARTICLE AR 89 2002 17 4 |
| allfields_unstemmed |
(DE-627)NLEJ250596881 (DE-601)aps:00f17e11d39ba7d72bae0e859dacee53a46d3975 DE-627 ger DE-627 rakwb Role of Molecular Size in Ratchet Fractionation 2002 Online-Ressource 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs. APS Digital Backfile Archive 1893-2003 Huang, Lotien Richard oth Silberzan, Pascal oth Tegenfeldt, Jonas O. oth Cox, Edward C. oth Sturm, James C. oth Austin, <?format <format:justify></format:justify>?>Robert H. oth Craighead, Harold oth Enthalten in Physical review letters College Park, Md. : APS, 1958 89(2002), 17 Online-Ressource (DE-627)NLEJ248237888 (DE-600)1472655-5 1079-7114 nnns volume:89 year:2002 number:17 extent:4 https://www.tib.eu/de/suchen/id/aps%3A00f17e11d39ba7d72bae0e859dacee53a46d3975 Verlag Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-APS GBV_NL_ARTICLE AR 89 2002 17 4 |
| allfieldsGer |
(DE-627)NLEJ250596881 (DE-601)aps:00f17e11d39ba7d72bae0e859dacee53a46d3975 DE-627 ger DE-627 rakwb Role of Molecular Size in Ratchet Fractionation 2002 Online-Ressource 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs. APS Digital Backfile Archive 1893-2003 Huang, Lotien Richard oth Silberzan, Pascal oth Tegenfeldt, Jonas O. oth Cox, Edward C. oth Sturm, James C. oth Austin, <?format <format:justify></format:justify>?>Robert H. oth Craighead, Harold oth Enthalten in Physical review letters College Park, Md. : APS, 1958 89(2002), 17 Online-Ressource (DE-627)NLEJ248237888 (DE-600)1472655-5 1079-7114 nnns volume:89 year:2002 number:17 extent:4 https://www.tib.eu/de/suchen/id/aps%3A00f17e11d39ba7d72bae0e859dacee53a46d3975 Verlag Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-APS GBV_NL_ARTICLE AR 89 2002 17 4 |
| allfieldsSound |
(DE-627)NLEJ250596881 (DE-601)aps:00f17e11d39ba7d72bae0e859dacee53a46d3975 DE-627 ger DE-627 rakwb Role of Molecular Size in Ratchet Fractionation 2002 Online-Ressource 4 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs. APS Digital Backfile Archive 1893-2003 Huang, Lotien Richard oth Silberzan, Pascal oth Tegenfeldt, Jonas O. oth Cox, Edward C. oth Sturm, James C. oth Austin, <?format <format:justify></format:justify>?>Robert H. oth Craighead, Harold oth Enthalten in Physical review letters College Park, Md. : APS, 1958 89(2002), 17 Online-Ressource (DE-627)NLEJ248237888 (DE-600)1472655-5 1079-7114 nnns volume:89 year:2002 number:17 extent:4 https://www.tib.eu/de/suchen/id/aps%3A00f17e11d39ba7d72bae0e859dacee53a46d3975 Verlag Deutschlandweit zugänglich GBV_USEFLAG_U ZDB-1-APS GBV_NL_ARTICLE AR 89 2002 17 4 |
| source |
Enthalten in Physical review letters 89(2002), 17 volume:89 year:2002 number:17 extent:4 |
| sourceStr |
Enthalten in Physical review letters 89(2002), 17 volume:89 year:2002 number:17 extent:4 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| isfreeaccess_bool |
false |
| container_title |
Physical review letters |
| authorswithroles_txt_mv |
Huang, Lotien Richard @@oth@@ Silberzan, Pascal @@oth@@ Tegenfeldt, Jonas O. @@oth@@ Cox, Edward C. @@oth@@ Sturm, James C. @@oth@@ Austin, <?format <format:justify></format:justify>?>Robert H. @@oth@@ Craighead, Harold @@oth@@ |
| publishDateDaySort_date |
2002-01-01T00:00:00Z |
| hierarchy_top_id |
NLEJ248237888 |
| id |
NLEJ250596881 |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">NLEJ250596881</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20231114105120.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">231114s2002 xx |||||o 00| ||und c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ250596881</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-601)aps:00f17e11d39ba7d72bae0e859dacee53a46d3975</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Role of Molecular Size in Ratchet Fractionation</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2002</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">4</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">APS Digital Backfile Archive 1893-2003</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Huang, Lotien Richard</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Silberzan, Pascal</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tegenfeldt, Jonas O.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cox, Edward C.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sturm, James C.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Austin, <?format <format:justify></format:justify>?>Robert H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Craighead, Harold</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Physical review letters</subfield><subfield code="d">College Park, Md. : APS, 1958</subfield><subfield code="g">89(2002), 17</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ248237888</subfield><subfield code="w">(DE-600)1472655-5</subfield><subfield code="x">1079-7114</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:89</subfield><subfield code="g">year:2002</subfield><subfield code="g">number:17</subfield><subfield code="g">extent:4</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.tib.eu/de/suchen/id/aps%3A00f17e11d39ba7d72bae0e859dacee53a46d3975</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-APS</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">89</subfield><subfield code="j">2002</subfield><subfield code="e">17</subfield><subfield code="g">4</subfield></datafield></record></collection>
|
| series2 |
APS Digital Backfile Archive 1893-2003 |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)NLEJ248237888 |
| format |
electronic Article |
| delete_txt_mv |
keep |
| collection |
NL |
| remote_str |
true |
| illustrated |
Not Illustrated |
| issn |
1079-7114 |
| topic_title |
Role of Molecular Size in Ratchet Fractionation |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
cr |
| author2_variant |
l r h lr lrh p s ps j o t jo jot e c c ec ecc j c s jc jcs f f h a ffh ffha h c hc |
| hierarchy_parent_title |
Physical review letters |
| hierarchy_parent_id |
NLEJ248237888 |
| hierarchy_top_title |
Physical review letters |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)NLEJ248237888 (DE-600)1472655-5 |
| title |
Role of Molecular Size in Ratchet Fractionation |
| spellingShingle |
Role of Molecular Size in Ratchet Fractionation |
| ctrlnum |
(DE-627)NLEJ250596881 (DE-601)aps:00f17e11d39ba7d72bae0e859dacee53a46d3975 |
| title_full |
Role of Molecular Size in Ratchet Fractionation |
| journal |
Physical review letters |
| journalStr |
Physical review letters |
| isOA_bool |
false |
| recordtype |
marc |
| publishDateSort |
2002 |
| contenttype_str_mv |
txt |
| container_volume |
89 |
| physical |
Online-Ressource 4 |
| format_se |
Elektronische Aufsätze |
| title_sort |
role of molecular size in ratchet fractionation |
| title_auth |
Role of Molecular Size in Ratchet Fractionation |
| abstract |
We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs. |
| abstractGer |
We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs. |
| abstract_unstemmed |
We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs. |
| collection_details |
GBV_USEFLAG_U ZDB-1-APS GBV_NL_ARTICLE |
| container_issue |
17 |
| title_short |
Role of Molecular Size in Ratchet Fractionation |
| url |
https://www.tib.eu/de/suchen/id/aps%3A00f17e11d39ba7d72bae0e859dacee53a46d3975 |
| remote_bool |
true |
| author2 |
Huang, Lotien Richard Silberzan, Pascal Tegenfeldt, Jonas O. Cox, Edward C. Sturm, James C. Austin, <?format <format:justify></format:justify>?>Robert H. Craighead, Harold |
| author2Str |
Huang, Lotien Richard Silberzan, Pascal Tegenfeldt, Jonas O. Cox, Edward C. Sturm, James C. Austin, <?format <format:justify></format:justify>?>Robert H. Craighead, Harold |
| ppnlink |
NLEJ248237888 |
| mediatype_str_mv |
c |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth oth oth oth |
| up_date |
2024-07-06T08:24:45.190Z |
| _version_ |
1803817357348438016 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">NLEJ250596881</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20231114105120.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">231114s2002 xx |||||o 00| ||und c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ250596881</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-601)aps:00f17e11d39ba7d72bae0e859dacee53a46d3975</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Role of Molecular Size in Ratchet Fractionation</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2002</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">4</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">We show the importance of finite particle size in microfluidic asymmetric continuous-flow diffusion arrays, specifically the critical nature of the particle size with respect to the barrier gaps. We show that particles much smaller than the barrier gap follow individual field lines through narrow gaps and are poorly fractionated. In contrast, particles comparable to the gap size lose memory of their incoming field line and can be fractionated with high resolution. We demonstrate this effect using a new technological approach to create very straight and narrow injection bands in such arrays, and completely resolve bands of DNA of lengths 48 500 and 167 000 base pairs.</subfield></datafield><datafield tag="533" ind1=" " ind2=" "><subfield code="f">APS Digital Backfile Archive 1893-2003</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Huang, Lotien Richard</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Silberzan, Pascal</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tegenfeldt, Jonas O.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cox, Edward C.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sturm, James C.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Austin, <?format <format:justify></format:justify>?>Robert H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Craighead, Harold</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Physical review letters</subfield><subfield code="d">College Park, Md. : APS, 1958</subfield><subfield code="g">89(2002), 17</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ248237888</subfield><subfield code="w">(DE-600)1472655-5</subfield><subfield code="x">1079-7114</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:89</subfield><subfield code="g">year:2002</subfield><subfield code="g">number:17</subfield><subfield code="g">extent:4</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.tib.eu/de/suchen/id/aps%3A00f17e11d39ba7d72bae0e859dacee53a46d3975</subfield><subfield code="x">Verlag</subfield><subfield code="z">Deutschlandweit zugänglich</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-APS</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">89</subfield><subfield code="j">2002</subfield><subfield code="e">17</subfield><subfield code="g">4</subfield></datafield></record></collection>
|
| score |
7.400728 |