Determination of L-Amino Acids and L-Amino Acid Oxidase Activity Using Luminol Chemiluminescence
A quantitative enzymatic method has been developed for analysis of most of the naturally occurring L-amino acids as well as the enzyme L-amino acid oxidase. The method is based on the reaction of L-amino acid oxidase with L-amino acids to generate hydrogen peroxide. The peroxide is then determined b...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lowery, Stephen [verfasserIn] Carr, Peter [verfasserIn] Seitz, W. Rudolf [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2011 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Analytical letters - New York, NY : Taylor & Francis, 1967, 10(1977), 12, Seite 931-943 |
|---|---|
| Übergeordnetes Werk: |
number:12 ; volume:10 ; year:1977 ; pages:931-943 |
| Links: |
|---|
| DOI / URN: |
10.1080/00032717708059249 |
|---|
| Katalog-ID: |
NLEJ251855341 |
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| 520 | |a A quantitative enzymatic method has been developed for analysis of most of the naturally occurring L-amino acids as well as the enzyme L-amino acid oxidase. The method is based on the reaction of L-amino acid oxidase with L-amino acids to generate hydrogen peroxide. The peroxide is then determined by reacting it with excess luminol and ferricyanide and measuring the resulting chemiluminescence. The lower limit of detection for any particular amino acid is related to the specific activity of the enzyme for that substrate. Concentrations as low as 5.0 × 10-8 M are measurable for some amino acids. Using L-phenylalanine as a substrate, enzyme activity can be measured in the range of 0.012 to 0.21 U. of enzyme. | ||
| 700 | 1 | |a Carr, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Seitz, W. Rudolf |e verfasserin |4 aut | |
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10.1080/00032717708059249 doi (DE-627)NLEJ251855341 (TFO)762574191 DE-627 ger DE-627 rda eng Lowery, Stephen verfasserin aut Determination of L-Amino Acids and L-Amino Acid Oxidase Activity Using Luminol Chemiluminescence 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A quantitative enzymatic method has been developed for analysis of most of the naturally occurring L-amino acids as well as the enzyme L-amino acid oxidase. The method is based on the reaction of L-amino acid oxidase with L-amino acids to generate hydrogen peroxide. The peroxide is then determined by reacting it with excess luminol and ferricyanide and measuring the resulting chemiluminescence. The lower limit of detection for any particular amino acid is related to the specific activity of the enzyme for that substrate. Concentrations as low as 5.0 × 10-8 M are measurable for some amino acids. Using L-phenylalanine as a substrate, enzyme activity can be measured in the range of 0.012 to 0.21 U. of enzyme. Carr, Peter verfasserin aut Seitz, W. Rudolf verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 10(1977), 12, Seite 931-943 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:12 volume:10 year:1977 pages:931-943 https://www.tib.eu/de/suchen/id/tandf%3A4abb4964b304016113e177ab900bbde7dc1d6788 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 12 10 1977 931-943 |
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10.1080/00032717708059249 doi (DE-627)NLEJ251855341 (TFO)762574191 DE-627 ger DE-627 rda eng Lowery, Stephen verfasserin aut Determination of L-Amino Acids and L-Amino Acid Oxidase Activity Using Luminol Chemiluminescence 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A quantitative enzymatic method has been developed for analysis of most of the naturally occurring L-amino acids as well as the enzyme L-amino acid oxidase. The method is based on the reaction of L-amino acid oxidase with L-amino acids to generate hydrogen peroxide. The peroxide is then determined by reacting it with excess luminol and ferricyanide and measuring the resulting chemiluminescence. The lower limit of detection for any particular amino acid is related to the specific activity of the enzyme for that substrate. Concentrations as low as 5.0 × 10-8 M are measurable for some amino acids. Using L-phenylalanine as a substrate, enzyme activity can be measured in the range of 0.012 to 0.21 U. of enzyme. Carr, Peter verfasserin aut Seitz, W. Rudolf verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 10(1977), 12, Seite 931-943 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:12 volume:10 year:1977 pages:931-943 https://www.tib.eu/de/suchen/id/tandf%3A4abb4964b304016113e177ab900bbde7dc1d6788 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 12 10 1977 931-943 |
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10.1080/00032717708059249 doi (DE-627)NLEJ251855341 (TFO)762574191 DE-627 ger DE-627 rda eng Lowery, Stephen verfasserin aut Determination of L-Amino Acids and L-Amino Acid Oxidase Activity Using Luminol Chemiluminescence 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A quantitative enzymatic method has been developed for analysis of most of the naturally occurring L-amino acids as well as the enzyme L-amino acid oxidase. The method is based on the reaction of L-amino acid oxidase with L-amino acids to generate hydrogen peroxide. The peroxide is then determined by reacting it with excess luminol and ferricyanide and measuring the resulting chemiluminescence. The lower limit of detection for any particular amino acid is related to the specific activity of the enzyme for that substrate. Concentrations as low as 5.0 × 10-8 M are measurable for some amino acids. Using L-phenylalanine as a substrate, enzyme activity can be measured in the range of 0.012 to 0.21 U. of enzyme. Carr, Peter verfasserin aut Seitz, W. Rudolf verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 10(1977), 12, Seite 931-943 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:12 volume:10 year:1977 pages:931-943 https://www.tib.eu/de/suchen/id/tandf%3A4abb4964b304016113e177ab900bbde7dc1d6788 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 12 10 1977 931-943 |
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10.1080/00032717708059249 doi (DE-627)NLEJ251855341 (TFO)762574191 DE-627 ger DE-627 rda eng Lowery, Stephen verfasserin aut Determination of L-Amino Acids and L-Amino Acid Oxidase Activity Using Luminol Chemiluminescence 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A quantitative enzymatic method has been developed for analysis of most of the naturally occurring L-amino acids as well as the enzyme L-amino acid oxidase. The method is based on the reaction of L-amino acid oxidase with L-amino acids to generate hydrogen peroxide. The peroxide is then determined by reacting it with excess luminol and ferricyanide and measuring the resulting chemiluminescence. The lower limit of detection for any particular amino acid is related to the specific activity of the enzyme for that substrate. Concentrations as low as 5.0 × 10-8 M are measurable for some amino acids. Using L-phenylalanine as a substrate, enzyme activity can be measured in the range of 0.012 to 0.21 U. of enzyme. Carr, Peter verfasserin aut Seitz, W. Rudolf verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 10(1977), 12, Seite 931-943 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:12 volume:10 year:1977 pages:931-943 https://www.tib.eu/de/suchen/id/tandf%3A4abb4964b304016113e177ab900bbde7dc1d6788 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 12 10 1977 931-943 |
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10.1080/00032717708059249 doi (DE-627)NLEJ251855341 (TFO)762574191 DE-627 ger DE-627 rda eng Lowery, Stephen verfasserin aut Determination of L-Amino Acids and L-Amino Acid Oxidase Activity Using Luminol Chemiluminescence 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A quantitative enzymatic method has been developed for analysis of most of the naturally occurring L-amino acids as well as the enzyme L-amino acid oxidase. The method is based on the reaction of L-amino acid oxidase with L-amino acids to generate hydrogen peroxide. The peroxide is then determined by reacting it with excess luminol and ferricyanide and measuring the resulting chemiluminescence. The lower limit of detection for any particular amino acid is related to the specific activity of the enzyme for that substrate. Concentrations as low as 5.0 × 10-8 M are measurable for some amino acids. Using L-phenylalanine as a substrate, enzyme activity can be measured in the range of 0.012 to 0.21 U. of enzyme. Carr, Peter verfasserin aut Seitz, W. Rudolf verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 10(1977), 12, Seite 931-943 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:12 volume:10 year:1977 pages:931-943 https://www.tib.eu/de/suchen/id/tandf%3A4abb4964b304016113e177ab900bbde7dc1d6788 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 12 10 1977 931-943 |
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Lowery, Stephen Determination of L-Amino Acids and L-Amino Acid Oxidase Activity Using Luminol Chemiluminescence |
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determination of l-amino acids and l-amino acid oxidase activity using luminol chemiluminescence |
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Determination of L-Amino Acids and L-Amino Acid Oxidase Activity Using Luminol Chemiluminescence |
| abstract |
A quantitative enzymatic method has been developed for analysis of most of the naturally occurring L-amino acids as well as the enzyme L-amino acid oxidase. The method is based on the reaction of L-amino acid oxidase with L-amino acids to generate hydrogen peroxide. The peroxide is then determined by reacting it with excess luminol and ferricyanide and measuring the resulting chemiluminescence. The lower limit of detection for any particular amino acid is related to the specific activity of the enzyme for that substrate. Concentrations as low as 5.0 × 10-8 M are measurable for some amino acids. Using L-phenylalanine as a substrate, enzyme activity can be measured in the range of 0.012 to 0.21 U. of enzyme. |
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A quantitative enzymatic method has been developed for analysis of most of the naturally occurring L-amino acids as well as the enzyme L-amino acid oxidase. The method is based on the reaction of L-amino acid oxidase with L-amino acids to generate hydrogen peroxide. The peroxide is then determined by reacting it with excess luminol and ferricyanide and measuring the resulting chemiluminescence. The lower limit of detection for any particular amino acid is related to the specific activity of the enzyme for that substrate. Concentrations as low as 5.0 × 10-8 M are measurable for some amino acids. Using L-phenylalanine as a substrate, enzyme activity can be measured in the range of 0.012 to 0.21 U. of enzyme. |
| abstract_unstemmed |
A quantitative enzymatic method has been developed for analysis of most of the naturally occurring L-amino acids as well as the enzyme L-amino acid oxidase. The method is based on the reaction of L-amino acid oxidase with L-amino acids to generate hydrogen peroxide. The peroxide is then determined by reacting it with excess luminol and ferricyanide and measuring the resulting chemiluminescence. The lower limit of detection for any particular amino acid is related to the specific activity of the enzyme for that substrate. Concentrations as low as 5.0 × 10-8 M are measurable for some amino acids. Using L-phenylalanine as a substrate, enzyme activity can be measured in the range of 0.012 to 0.21 U. of enzyme. |
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Determination of L-Amino Acids and L-Amino Acid Oxidase Activity Using Luminol Chemiluminescence |
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