Immobilization of Enzymes on Polychlorotrifluoroethylene Particles Packed in Small Columns
The use of polychlorotrifluoroethylene (PCTFE) particles packed in 5-10 cm × 0.4 cm ID columns for the immobilization of enzymes by hydrophobic adsorption is investigated. Enzyme binding capacity of PCTFE is about 0.2 mg/gm of polymer. PCTFE-immobilized lactate dehydrogenase, alcohol dehydrogenase,...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Danielson, N. D. [verfasserIn] Bossu, T. M. [verfasserIn] Kruempelman, M. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2011 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Analytical letters - New York, NY : Taylor & Francis, 1967, 15(1982), 16, Seite 1289-1300 |
|---|---|
| Übergeordnetes Werk: |
number:16 ; volume:15 ; year:1982 ; pages:1289-1300 |
| Links: |
|---|
| DOI / URN: |
10.1080/00032718208069534 |
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NLEJ251862704 |
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| 520 | |a The use of polychlorotrifluoroethylene (PCTFE) particles packed in 5-10 cm × 0.4 cm ID columns for the immobilization of enzymes by hydrophobic adsorption is investigated. Enzyme binding capacity of PCTFE is about 0.2 mg/gm of polymer. PCTFE-immobilized lactate dehydrogenase, alcohol dehydrogenase, and urease-glutamate dehydrogenase are demonstrated to be useful for the determinations of pyruvate, ethanol, and urea, respectively. Immobilized enzyme lifetimes are generally about 1-2 months. | ||
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10.1080/00032718208069534 doi (DE-627)NLEJ251862704 (TFO)768007388 DE-627 ger DE-627 rda eng Danielson, N. D. verfasserin aut Immobilization of Enzymes on Polychlorotrifluoroethylene Particles Packed in Small Columns 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The use of polychlorotrifluoroethylene (PCTFE) particles packed in 5-10 cm × 0.4 cm ID columns for the immobilization of enzymes by hydrophobic adsorption is investigated. Enzyme binding capacity of PCTFE is about 0.2 mg/gm of polymer. PCTFE-immobilized lactate dehydrogenase, alcohol dehydrogenase, and urease-glutamate dehydrogenase are demonstrated to be useful for the determinations of pyruvate, ethanol, and urea, respectively. Immobilized enzyme lifetimes are generally about 1-2 months. Bossu, T. M. verfasserin aut Kruempelman, M. verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 15(1982), 16, Seite 1289-1300 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:16 volume:15 year:1982 pages:1289-1300 https://www.tib.eu/de/suchen/id/tandf%3Ad974a4b9983e782074ef9a453087a898c7d668f9 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 16 15 1982 1289-1300 |
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10.1080/00032718208069534 doi (DE-627)NLEJ251862704 (TFO)768007388 DE-627 ger DE-627 rda eng Danielson, N. D. verfasserin aut Immobilization of Enzymes on Polychlorotrifluoroethylene Particles Packed in Small Columns 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The use of polychlorotrifluoroethylene (PCTFE) particles packed in 5-10 cm × 0.4 cm ID columns for the immobilization of enzymes by hydrophobic adsorption is investigated. Enzyme binding capacity of PCTFE is about 0.2 mg/gm of polymer. PCTFE-immobilized lactate dehydrogenase, alcohol dehydrogenase, and urease-glutamate dehydrogenase are demonstrated to be useful for the determinations of pyruvate, ethanol, and urea, respectively. Immobilized enzyme lifetimes are generally about 1-2 months. Bossu, T. M. verfasserin aut Kruempelman, M. verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 15(1982), 16, Seite 1289-1300 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:16 volume:15 year:1982 pages:1289-1300 https://www.tib.eu/de/suchen/id/tandf%3Ad974a4b9983e782074ef9a453087a898c7d668f9 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 16 15 1982 1289-1300 |
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10.1080/00032718208069534 doi (DE-627)NLEJ251862704 (TFO)768007388 DE-627 ger DE-627 rda eng Danielson, N. D. verfasserin aut Immobilization of Enzymes on Polychlorotrifluoroethylene Particles Packed in Small Columns 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The use of polychlorotrifluoroethylene (PCTFE) particles packed in 5-10 cm × 0.4 cm ID columns for the immobilization of enzymes by hydrophobic adsorption is investigated. Enzyme binding capacity of PCTFE is about 0.2 mg/gm of polymer. PCTFE-immobilized lactate dehydrogenase, alcohol dehydrogenase, and urease-glutamate dehydrogenase are demonstrated to be useful for the determinations of pyruvate, ethanol, and urea, respectively. Immobilized enzyme lifetimes are generally about 1-2 months. Bossu, T. M. verfasserin aut Kruempelman, M. verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 15(1982), 16, Seite 1289-1300 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:16 volume:15 year:1982 pages:1289-1300 https://www.tib.eu/de/suchen/id/tandf%3Ad974a4b9983e782074ef9a453087a898c7d668f9 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 16 15 1982 1289-1300 |
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10.1080/00032718208069534 doi (DE-627)NLEJ251862704 (TFO)768007388 DE-627 ger DE-627 rda eng Danielson, N. D. verfasserin aut Immobilization of Enzymes on Polychlorotrifluoroethylene Particles Packed in Small Columns 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The use of polychlorotrifluoroethylene (PCTFE) particles packed in 5-10 cm × 0.4 cm ID columns for the immobilization of enzymes by hydrophobic adsorption is investigated. Enzyme binding capacity of PCTFE is about 0.2 mg/gm of polymer. PCTFE-immobilized lactate dehydrogenase, alcohol dehydrogenase, and urease-glutamate dehydrogenase are demonstrated to be useful for the determinations of pyruvate, ethanol, and urea, respectively. Immobilized enzyme lifetimes are generally about 1-2 months. Bossu, T. M. verfasserin aut Kruempelman, M. verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 15(1982), 16, Seite 1289-1300 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:16 volume:15 year:1982 pages:1289-1300 https://www.tib.eu/de/suchen/id/tandf%3Ad974a4b9983e782074ef9a453087a898c7d668f9 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 16 15 1982 1289-1300 |
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10.1080/00032718208069534 doi (DE-627)NLEJ251862704 (TFO)768007388 DE-627 ger DE-627 rda eng Danielson, N. D. verfasserin aut Immobilization of Enzymes on Polychlorotrifluoroethylene Particles Packed in Small Columns 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The use of polychlorotrifluoroethylene (PCTFE) particles packed in 5-10 cm × 0.4 cm ID columns for the immobilization of enzymes by hydrophobic adsorption is investigated. Enzyme binding capacity of PCTFE is about 0.2 mg/gm of polymer. PCTFE-immobilized lactate dehydrogenase, alcohol dehydrogenase, and urease-glutamate dehydrogenase are demonstrated to be useful for the determinations of pyruvate, ethanol, and urea, respectively. Immobilized enzyme lifetimes are generally about 1-2 months. Bossu, T. M. verfasserin aut Kruempelman, M. verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 15(1982), 16, Seite 1289-1300 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:16 volume:15 year:1982 pages:1289-1300 https://www.tib.eu/de/suchen/id/tandf%3Ad974a4b9983e782074ef9a453087a898c7d668f9 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 16 15 1982 1289-1300 |
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immobilization of enzymes on polychlorotrifluoroethylene particles packed in small columns |
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Immobilization of Enzymes on Polychlorotrifluoroethylene Particles Packed in Small Columns |
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The use of polychlorotrifluoroethylene (PCTFE) particles packed in 5-10 cm × 0.4 cm ID columns for the immobilization of enzymes by hydrophobic adsorption is investigated. Enzyme binding capacity of PCTFE is about 0.2 mg/gm of polymer. PCTFE-immobilized lactate dehydrogenase, alcohol dehydrogenase, and urease-glutamate dehydrogenase are demonstrated to be useful for the determinations of pyruvate, ethanol, and urea, respectively. Immobilized enzyme lifetimes are generally about 1-2 months. |
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The use of polychlorotrifluoroethylene (PCTFE) particles packed in 5-10 cm × 0.4 cm ID columns for the immobilization of enzymes by hydrophobic adsorption is investigated. Enzyme binding capacity of PCTFE is about 0.2 mg/gm of polymer. PCTFE-immobilized lactate dehydrogenase, alcohol dehydrogenase, and urease-glutamate dehydrogenase are demonstrated to be useful for the determinations of pyruvate, ethanol, and urea, respectively. Immobilized enzyme lifetimes are generally about 1-2 months. |
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The use of polychlorotrifluoroethylene (PCTFE) particles packed in 5-10 cm × 0.4 cm ID columns for the immobilization of enzymes by hydrophobic adsorption is investigated. Enzyme binding capacity of PCTFE is about 0.2 mg/gm of polymer. PCTFE-immobilized lactate dehydrogenase, alcohol dehydrogenase, and urease-glutamate dehydrogenase are demonstrated to be useful for the determinations of pyruvate, ethanol, and urea, respectively. Immobilized enzyme lifetimes are generally about 1-2 months. |
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