A Simultaneous Enzyme-Linked Immunoassay for Human Thyroglobulin
A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Endo, Yuichi [verfasserIn] Ohtaki, Sachiya [verfasserIn] Kan, Hiroaki [verfasserIn] Yoshitake, Shinji [verfasserIn] Ishikawa, Eiji [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2011 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Analytical letters - New York, NY : Taylor & Francis, 1967, 15(1982), 16, Seite 1301-1315 |
|---|---|
| Übergeordnetes Werk: |
number:16 ; volume:15 ; year:1982 ; pages:1301-1315 |
| Links: |
|---|
| DOI / URN: |
10.1080/00032718208069535 |
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| 520 | |a A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase conjugate and serum sample were mixed at the same time. In order to improve the assay performance, an appropriate amount of Fab'-β-D-galactosidase conjugate was carefully chosen.The present assay was simple, rapid, highly sensitive and excellently reproducible. The sensitivity of the assay was approximately 1.5 amol/tube corresponding to 0.5 ng/ml using 2 μ1 of serum, which was higher than that in the two-step sandwich enzyme-linked immunoassay previously reported. The coefficients of variation for different levels of serum thyroglobulin determined by the present assay ranged from 3.4-7.2% and 2.5-6.1 % for within and between run, respectively. | ||
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10.1080/00032718208069535 doi (DE-627)NLEJ251862712 (TFO)768007389 DE-627 ger DE-627 rda eng Endo, Yuichi verfasserin aut A Simultaneous Enzyme-Linked Immunoassay for Human Thyroglobulin 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase conjugate and serum sample were mixed at the same time. In order to improve the assay performance, an appropriate amount of Fab'-β-D-galactosidase conjugate was carefully chosen.The present assay was simple, rapid, highly sensitive and excellently reproducible. The sensitivity of the assay was approximately 1.5 amol/tube corresponding to 0.5 ng/ml using 2 μ1 of serum, which was higher than that in the two-step sandwich enzyme-linked immunoassay previously reported. The coefficients of variation for different levels of serum thyroglobulin determined by the present assay ranged from 3.4-7.2% and 2.5-6.1 % for within and between run, respectively. Ohtaki, Sachiya verfasserin aut Kan, Hiroaki verfasserin aut Yoshitake, Shinji verfasserin aut Ishikawa, Eiji verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 15(1982), 16, Seite 1301-1315 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:16 volume:15 year:1982 pages:1301-1315 https://www.tib.eu/de/suchen/id/tandf%3Afdfd731730ebb8a6c1cae4cd9616dea30b00cd11 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 16 15 1982 1301-1315 |
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10.1080/00032718208069535 doi (DE-627)NLEJ251862712 (TFO)768007389 DE-627 ger DE-627 rda eng Endo, Yuichi verfasserin aut A Simultaneous Enzyme-Linked Immunoassay for Human Thyroglobulin 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase conjugate and serum sample were mixed at the same time. In order to improve the assay performance, an appropriate amount of Fab'-β-D-galactosidase conjugate was carefully chosen.The present assay was simple, rapid, highly sensitive and excellently reproducible. The sensitivity of the assay was approximately 1.5 amol/tube corresponding to 0.5 ng/ml using 2 μ1 of serum, which was higher than that in the two-step sandwich enzyme-linked immunoassay previously reported. The coefficients of variation for different levels of serum thyroglobulin determined by the present assay ranged from 3.4-7.2% and 2.5-6.1 % for within and between run, respectively. Ohtaki, Sachiya verfasserin aut Kan, Hiroaki verfasserin aut Yoshitake, Shinji verfasserin aut Ishikawa, Eiji verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 15(1982), 16, Seite 1301-1315 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:16 volume:15 year:1982 pages:1301-1315 https://www.tib.eu/de/suchen/id/tandf%3Afdfd731730ebb8a6c1cae4cd9616dea30b00cd11 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 16 15 1982 1301-1315 |
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10.1080/00032718208069535 doi (DE-627)NLEJ251862712 (TFO)768007389 DE-627 ger DE-627 rda eng Endo, Yuichi verfasserin aut A Simultaneous Enzyme-Linked Immunoassay for Human Thyroglobulin 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase conjugate and serum sample were mixed at the same time. In order to improve the assay performance, an appropriate amount of Fab'-β-D-galactosidase conjugate was carefully chosen.The present assay was simple, rapid, highly sensitive and excellently reproducible. The sensitivity of the assay was approximately 1.5 amol/tube corresponding to 0.5 ng/ml using 2 μ1 of serum, which was higher than that in the two-step sandwich enzyme-linked immunoassay previously reported. The coefficients of variation for different levels of serum thyroglobulin determined by the present assay ranged from 3.4-7.2% and 2.5-6.1 % for within and between run, respectively. Ohtaki, Sachiya verfasserin aut Kan, Hiroaki verfasserin aut Yoshitake, Shinji verfasserin aut Ishikawa, Eiji verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 15(1982), 16, Seite 1301-1315 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:16 volume:15 year:1982 pages:1301-1315 https://www.tib.eu/de/suchen/id/tandf%3Afdfd731730ebb8a6c1cae4cd9616dea30b00cd11 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 16 15 1982 1301-1315 |
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10.1080/00032718208069535 doi (DE-627)NLEJ251862712 (TFO)768007389 DE-627 ger DE-627 rda eng Endo, Yuichi verfasserin aut A Simultaneous Enzyme-Linked Immunoassay for Human Thyroglobulin 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase conjugate and serum sample were mixed at the same time. In order to improve the assay performance, an appropriate amount of Fab'-β-D-galactosidase conjugate was carefully chosen.The present assay was simple, rapid, highly sensitive and excellently reproducible. The sensitivity of the assay was approximately 1.5 amol/tube corresponding to 0.5 ng/ml using 2 μ1 of serum, which was higher than that in the two-step sandwich enzyme-linked immunoassay previously reported. The coefficients of variation for different levels of serum thyroglobulin determined by the present assay ranged from 3.4-7.2% and 2.5-6.1 % for within and between run, respectively. Ohtaki, Sachiya verfasserin aut Kan, Hiroaki verfasserin aut Yoshitake, Shinji verfasserin aut Ishikawa, Eiji verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 15(1982), 16, Seite 1301-1315 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:16 volume:15 year:1982 pages:1301-1315 https://www.tib.eu/de/suchen/id/tandf%3Afdfd731730ebb8a6c1cae4cd9616dea30b00cd11 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 16 15 1982 1301-1315 |
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10.1080/00032718208069535 doi (DE-627)NLEJ251862712 (TFO)768007389 DE-627 ger DE-627 rda eng Endo, Yuichi verfasserin aut A Simultaneous Enzyme-Linked Immunoassay for Human Thyroglobulin 2011 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase conjugate and serum sample were mixed at the same time. In order to improve the assay performance, an appropriate amount of Fab'-β-D-galactosidase conjugate was carefully chosen.The present assay was simple, rapid, highly sensitive and excellently reproducible. The sensitivity of the assay was approximately 1.5 amol/tube corresponding to 0.5 ng/ml using 2 μ1 of serum, which was higher than that in the two-step sandwich enzyme-linked immunoassay previously reported. The coefficients of variation for different levels of serum thyroglobulin determined by the present assay ranged from 3.4-7.2% and 2.5-6.1 % for within and between run, respectively. Ohtaki, Sachiya verfasserin aut Kan, Hiroaki verfasserin aut Yoshitake, Shinji verfasserin aut Ishikawa, Eiji verfasserin aut Enthalten in Analytical letters New York, NY : Taylor & Francis, 1967 15(1982), 16, Seite 1301-1315 Online-Ressource (DE-627)NLEJ251855147 (DE-600)2098207-0 (DE-576)263254321 1532-236X nnns number:16 volume:15 year:1982 pages:1301-1315 https://www.tib.eu/de/suchen/id/tandf%3Afdfd731730ebb8a6c1cae4cd9616dea30b00cd11 Digitalisierung Deutschlandweit zugänglich ZDB-1-TFO GBV_NL_ARTICLE AR 16 15 1982 1301-1315 |
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A Simultaneous Enzyme-Linked Immunoassay for Human Thyroglobulin |
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A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase conjugate and serum sample were mixed at the same time. In order to improve the assay performance, an appropriate amount of Fab'-β-D-galactosidase conjugate was carefully chosen.The present assay was simple, rapid, highly sensitive and excellently reproducible. The sensitivity of the assay was approximately 1.5 amol/tube corresponding to 0.5 ng/ml using 2 μ1 of serum, which was higher than that in the two-step sandwich enzyme-linked immunoassay previously reported. The coefficients of variation for different levels of serum thyroglobulin determined by the present assay ranged from 3.4-7.2% and 2.5-6.1 % for within and between run, respectively. |
| abstractGer |
A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase conjugate and serum sample were mixed at the same time. In order to improve the assay performance, an appropriate amount of Fab'-β-D-galactosidase conjugate was carefully chosen.The present assay was simple, rapid, highly sensitive and excellently reproducible. The sensitivity of the assay was approximately 1.5 amol/tube corresponding to 0.5 ng/ml using 2 μ1 of serum, which was higher than that in the two-step sandwich enzyme-linked immunoassay previously reported. The coefficients of variation for different levels of serum thyroglobulin determined by the present assay ranged from 3.4-7.2% and 2.5-6.1 % for within and between run, respectively. |
| abstract_unstemmed |
A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase conjugate and serum sample were mixed at the same time. In order to improve the assay performance, an appropriate amount of Fab'-β-D-galactosidase conjugate was carefully chosen.The present assay was simple, rapid, highly sensitive and excellently reproducible. The sensitivity of the assay was approximately 1.5 amol/tube corresponding to 0.5 ng/ml using 2 μ1 of serum, which was higher than that in the two-step sandwich enzyme-linked immunoassay previously reported. The coefficients of variation for different levels of serum thyroglobulin determined by the present assay ranged from 3.4-7.2% and 2.5-6.1 % for within and between run, respectively. |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">NLEJ251862712</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20231206140753.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">231206s2011 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1080/00032718208069535</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)NLEJ251862712</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(TFO)768007389</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Endo, Yuichi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">A Simultaneous Enzyme-Linked Immunoassay for Human Thyroglobulin</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2011</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A “simultaneous” enzyme-linked immunoassay for the measurement of thyroglobulin in human serum was established using polyclonal rabbit antibody, in which rabbit anti-human thyroglobulin IgG-coated silicone rubber rod, rabbit anti-human thyroglobulin monovalent fragment (Fab')-β-D-galactosidase conjugate and serum sample were mixed at the same time. In order to improve the assay performance, an appropriate amount of Fab'-β-D-galactosidase conjugate was carefully chosen.The present assay was simple, rapid, highly sensitive and excellently reproducible. The sensitivity of the assay was approximately 1.5 amol/tube corresponding to 0.5 ng/ml using 2 μ1 of serum, which was higher than that in the two-step sandwich enzyme-linked immunoassay previously reported. The coefficients of variation for different levels of serum thyroglobulin determined by the present assay ranged from 3.4-7.2% and 2.5-6.1 % for within and between run, respectively.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ohtaki, Sachiya</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kan, Hiroaki</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yoshitake, Shinji</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ishikawa, Eiji</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Analytical letters</subfield><subfield code="d">New York, NY : Taylor & Francis, 1967</subfield><subfield code="g">15(1982), 16, Seite 1301-1315</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)NLEJ251855147</subfield><subfield code="w">(DE-600)2098207-0</subfield><subfield code="w">(DE-576)263254321</subfield><subfield code="x">1532-236X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">number:16</subfield><subfield code="g">volume:15</subfield><subfield code="g">year:1982</subfield><subfield code="g">pages:1301-1315</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.tib.eu/de/suchen/id/tandf%3Afdfd731730ebb8a6c1cae4cd9616dea30b00cd11</subfield><subfield code="x">Digitalisierung</subfield><subfield code="z">Deutschlandweit zugänglich</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">ZDB-1-TFO</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_NL_ARTICLE</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="e">16</subfield><subfield code="d">15</subfield><subfield code="j">1982</subfield><subfield code="h">1301-1315</subfield></datafield></record></collection>
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