Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training

High efficiency separations are needed to enhance selectivity, mass spectral quality, and quantitative performance in metabolomic studies. However, low sample throughput and complicated data preprocessing remain major bottlenecks to biomarker discovery. We introduce an accelerated data workflow to i...
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Autor*in:	Kuehnbaum, Naomi L [verfasserIn] Gillen, Jenna B Kormendi, Aleshia Lam, Karen P DiBattista, Alicia Gibala, Martin J Britz‐McKibbin, Philip

Format:	Artikel
Sprache:	Englisch

Erschienen:	2015

Rechteinformationen:	Nutzungsrecht: © 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim © COPYRIGHT 2015 Wiley Subscription Services, Inc.

Schlagwörter:	Mass spectrometry Exercise training Capillary electrophoresis Biomarker discovery Metabolomics Antioxidants Cysteine Training Analysis Physiological aspects Levocarnitine Metabolites Purines Working women

Übergeordnetes Werk:	Enthalten in: Electrophoresis - Weinheim : Wiley-VCH, 1980, 36(2015), 18, Seite 2226-2236
Übergeordnetes Werk:	volume:36 ; year:2015 ; number:18 ; pages:2226-2236

Links:	Volltext Link aufrufen

DOI / URN:	10.1002/elps.201400604

Katalog-ID:	OLC1958970247

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540			\|a Nutzungsrecht: © 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
540			\|a © COPYRIGHT 2015 Wiley Subscription Services, Inc.
650		4	\|a Mass spectrometry
650		4	\|a Exercise training
650		4	\|a Capillary electrophoresis
650		4	\|a Biomarker discovery
650		4	\|a Metabolomics
650		4	\|a Antioxidants
650		4	\|a Cysteine
650		4	\|a Training
650		4	\|a Analysis
650		4	\|a Physiological aspects
650		4	\|a Levocarnitine
650		4	\|a Metabolites
650		4	\|a Purines
650		4	\|a Working women
700	1		\|a Gillen, Jenna B \|4 oth
700	1		\|a Kormendi, Aleshia \|4 oth
700	1		\|a Lam, Karen P \|4 oth
700	1		\|a DiBattista, Alicia \|4 oth
700	1		\|a Gibala, Martin J \|4 oth
700	1		\|a Britz‐McKibbin, Philip \|4 oth
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allfields	10.1002/elps.201400604 doi PQ20160617 (DE-627)OLC1958970247 (DE-599)GBVOLC1958970247 (PRQ)g1573-c36b166373d395cfaa454c55790fa7f0402764510f860821c9ccc6712b4578013 (KEY)0204026320150000036001802226multiplexedseparationsforbiomarkerdiscoveryinmetab DE-627 ger DE-627 rakwb eng 540 570 DNB 570 AVZ BIODIV fid 35.29 bkl Kuehnbaum, Naomi L verfasserin aut Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier High efficiency separations are needed to enhance selectivity, mass spectral quality, and quantitative performance in metabolomic studies. However, low sample throughput and complicated data preprocessing remain major bottlenecks to biomarker discovery. We introduce an accelerated data workflow to identify plasma metabolite signatures of exercise responsiveness when using multisegment injection‐capillary electrophoresis‐mass spectrometry (MSI‐CE‐MS). This multiplexed separation platform takes advantage of customizable serial injections to enhance sample throughput and data fidelity based on temporally resolved ion signals derived from seven different sample segments analyzed within a single run. MSI‐CE‐MS was applied to explore the adaptive metabolic responses of a cohort of overweight/obese women ( BMI > 25, n = 9) performing a 6‐wk high‐intensity interval training intervention using a repeated measures/cross‐over study design. Venous blood samples were collected from each subject at three time intervals (baseline, postexercise, recovery) in their naïve and trained states while completing standardized cycling trials at the same absolute workload. Complementary statistical methods were used to classify dynamic changes in plasma metabolism associated with strenuous exercise and training status. Positive adaptations to exercise were associated with training‐induced upregulation in plasma l ‐carnitine at rest due to improved muscle oxidative capacity, and greater antioxidant capacity as reflected by lower circulating glutathionyl‐ l ‐cysteine mixed disulfide. Attenuation in plasma hypoxanthine and higher O ‐acetyl‐ l ‐carnitine levels postexercise also indicated lower energetic stress for trained women. Nutzungsrecht: © 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim © COPYRIGHT 2015 Wiley Subscription Services, Inc. Mass spectrometry Exercise training Capillary electrophoresis Biomarker discovery Metabolomics Antioxidants Cysteine Training Analysis Physiological aspects Levocarnitine Metabolites Purines Working women Gillen, Jenna B oth Kormendi, Aleshia oth Lam, Karen P oth DiBattista, Alicia oth Gibala, Martin J oth Britz‐McKibbin, Philip oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 36(2015), 18, Seite 2226-2236 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:36 year:2015 number:18 pages:2226-2236 http://dx.doi.org/10.1002/elps.201400604 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201400604/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 36 2015 18 2226-2236
spelling	10.1002/elps.201400604 doi PQ20160617 (DE-627)OLC1958970247 (DE-599)GBVOLC1958970247 (PRQ)g1573-c36b166373d395cfaa454c55790fa7f0402764510f860821c9ccc6712b4578013 (KEY)0204026320150000036001802226multiplexedseparationsforbiomarkerdiscoveryinmetab DE-627 ger DE-627 rakwb eng 540 570 DNB 570 AVZ BIODIV fid 35.29 bkl Kuehnbaum, Naomi L verfasserin aut Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier High efficiency separations are needed to enhance selectivity, mass spectral quality, and quantitative performance in metabolomic studies. However, low sample throughput and complicated data preprocessing remain major bottlenecks to biomarker discovery. We introduce an accelerated data workflow to identify plasma metabolite signatures of exercise responsiveness when using multisegment injection‐capillary electrophoresis‐mass spectrometry (MSI‐CE‐MS). This multiplexed separation platform takes advantage of customizable serial injections to enhance sample throughput and data fidelity based on temporally resolved ion signals derived from seven different sample segments analyzed within a single run. MSI‐CE‐MS was applied to explore the adaptive metabolic responses of a cohort of overweight/obese women ( BMI > 25, n = 9) performing a 6‐wk high‐intensity interval training intervention using a repeated measures/cross‐over study design. Venous blood samples were collected from each subject at three time intervals (baseline, postexercise, recovery) in their naïve and trained states while completing standardized cycling trials at the same absolute workload. Complementary statistical methods were used to classify dynamic changes in plasma metabolism associated with strenuous exercise and training status. Positive adaptations to exercise were associated with training‐induced upregulation in plasma l ‐carnitine at rest due to improved muscle oxidative capacity, and greater antioxidant capacity as reflected by lower circulating glutathionyl‐ l ‐cysteine mixed disulfide. Attenuation in plasma hypoxanthine and higher O ‐acetyl‐ l ‐carnitine levels postexercise also indicated lower energetic stress for trained women. Nutzungsrecht: © 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim © COPYRIGHT 2015 Wiley Subscription Services, Inc. Mass spectrometry Exercise training Capillary electrophoresis Biomarker discovery Metabolomics Antioxidants Cysteine Training Analysis Physiological aspects Levocarnitine Metabolites Purines Working women Gillen, Jenna B oth Kormendi, Aleshia oth Lam, Karen P oth DiBattista, Alicia oth Gibala, Martin J oth Britz‐McKibbin, Philip oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 36(2015), 18, Seite 2226-2236 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:36 year:2015 number:18 pages:2226-2236 http://dx.doi.org/10.1002/elps.201400604 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201400604/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 36 2015 18 2226-2236
allfields_unstemmed	10.1002/elps.201400604 doi PQ20160617 (DE-627)OLC1958970247 (DE-599)GBVOLC1958970247 (PRQ)g1573-c36b166373d395cfaa454c55790fa7f0402764510f860821c9ccc6712b4578013 (KEY)0204026320150000036001802226multiplexedseparationsforbiomarkerdiscoveryinmetab DE-627 ger DE-627 rakwb eng 540 570 DNB 570 AVZ BIODIV fid 35.29 bkl Kuehnbaum, Naomi L verfasserin aut Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier High efficiency separations are needed to enhance selectivity, mass spectral quality, and quantitative performance in metabolomic studies. However, low sample throughput and complicated data preprocessing remain major bottlenecks to biomarker discovery. We introduce an accelerated data workflow to identify plasma metabolite signatures of exercise responsiveness when using multisegment injection‐capillary electrophoresis‐mass spectrometry (MSI‐CE‐MS). This multiplexed separation platform takes advantage of customizable serial injections to enhance sample throughput and data fidelity based on temporally resolved ion signals derived from seven different sample segments analyzed within a single run. MSI‐CE‐MS was applied to explore the adaptive metabolic responses of a cohort of overweight/obese women ( BMI > 25, n = 9) performing a 6‐wk high‐intensity interval training intervention using a repeated measures/cross‐over study design. Venous blood samples were collected from each subject at three time intervals (baseline, postexercise, recovery) in their naïve and trained states while completing standardized cycling trials at the same absolute workload. Complementary statistical methods were used to classify dynamic changes in plasma metabolism associated with strenuous exercise and training status. Positive adaptations to exercise were associated with training‐induced upregulation in plasma l ‐carnitine at rest due to improved muscle oxidative capacity, and greater antioxidant capacity as reflected by lower circulating glutathionyl‐ l ‐cysteine mixed disulfide. Attenuation in plasma hypoxanthine and higher O ‐acetyl‐ l ‐carnitine levels postexercise also indicated lower energetic stress for trained women. Nutzungsrecht: © 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim © COPYRIGHT 2015 Wiley Subscription Services, Inc. Mass spectrometry Exercise training Capillary electrophoresis Biomarker discovery Metabolomics Antioxidants Cysteine Training Analysis Physiological aspects Levocarnitine Metabolites Purines Working women Gillen, Jenna B oth Kormendi, Aleshia oth Lam, Karen P oth DiBattista, Alicia oth Gibala, Martin J oth Britz‐McKibbin, Philip oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 36(2015), 18, Seite 2226-2236 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:36 year:2015 number:18 pages:2226-2236 http://dx.doi.org/10.1002/elps.201400604 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201400604/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 36 2015 18 2226-2236
allfieldsGer	10.1002/elps.201400604 doi PQ20160617 (DE-627)OLC1958970247 (DE-599)GBVOLC1958970247 (PRQ)g1573-c36b166373d395cfaa454c55790fa7f0402764510f860821c9ccc6712b4578013 (KEY)0204026320150000036001802226multiplexedseparationsforbiomarkerdiscoveryinmetab DE-627 ger DE-627 rakwb eng 540 570 DNB 570 AVZ BIODIV fid 35.29 bkl Kuehnbaum, Naomi L verfasserin aut Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier High efficiency separations are needed to enhance selectivity, mass spectral quality, and quantitative performance in metabolomic studies. However, low sample throughput and complicated data preprocessing remain major bottlenecks to biomarker discovery. We introduce an accelerated data workflow to identify plasma metabolite signatures of exercise responsiveness when using multisegment injection‐capillary electrophoresis‐mass spectrometry (MSI‐CE‐MS). This multiplexed separation platform takes advantage of customizable serial injections to enhance sample throughput and data fidelity based on temporally resolved ion signals derived from seven different sample segments analyzed within a single run. MSI‐CE‐MS was applied to explore the adaptive metabolic responses of a cohort of overweight/obese women ( BMI > 25, n = 9) performing a 6‐wk high‐intensity interval training intervention using a repeated measures/cross‐over study design. Venous blood samples were collected from each subject at three time intervals (baseline, postexercise, recovery) in their naïve and trained states while completing standardized cycling trials at the same absolute workload. Complementary statistical methods were used to classify dynamic changes in plasma metabolism associated with strenuous exercise and training status. Positive adaptations to exercise were associated with training‐induced upregulation in plasma l ‐carnitine at rest due to improved muscle oxidative capacity, and greater antioxidant capacity as reflected by lower circulating glutathionyl‐ l ‐cysteine mixed disulfide. Attenuation in plasma hypoxanthine and higher O ‐acetyl‐ l ‐carnitine levels postexercise also indicated lower energetic stress for trained women. Nutzungsrecht: © 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim © COPYRIGHT 2015 Wiley Subscription Services, Inc. Mass spectrometry Exercise training Capillary electrophoresis Biomarker discovery Metabolomics Antioxidants Cysteine Training Analysis Physiological aspects Levocarnitine Metabolites Purines Working women Gillen, Jenna B oth Kormendi, Aleshia oth Lam, Karen P oth DiBattista, Alicia oth Gibala, Martin J oth Britz‐McKibbin, Philip oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 36(2015), 18, Seite 2226-2236 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:36 year:2015 number:18 pages:2226-2236 http://dx.doi.org/10.1002/elps.201400604 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201400604/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 36 2015 18 2226-2236
allfieldsSound	10.1002/elps.201400604 doi PQ20160617 (DE-627)OLC1958970247 (DE-599)GBVOLC1958970247 (PRQ)g1573-c36b166373d395cfaa454c55790fa7f0402764510f860821c9ccc6712b4578013 (KEY)0204026320150000036001802226multiplexedseparationsforbiomarkerdiscoveryinmetab DE-627 ger DE-627 rakwb eng 540 570 DNB 570 AVZ BIODIV fid 35.29 bkl Kuehnbaum, Naomi L verfasserin aut Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier High efficiency separations are needed to enhance selectivity, mass spectral quality, and quantitative performance in metabolomic studies. However, low sample throughput and complicated data preprocessing remain major bottlenecks to biomarker discovery. We introduce an accelerated data workflow to identify plasma metabolite signatures of exercise responsiveness when using multisegment injection‐capillary electrophoresis‐mass spectrometry (MSI‐CE‐MS). This multiplexed separation platform takes advantage of customizable serial injections to enhance sample throughput and data fidelity based on temporally resolved ion signals derived from seven different sample segments analyzed within a single run. MSI‐CE‐MS was applied to explore the adaptive metabolic responses of a cohort of overweight/obese women ( BMI > 25, n = 9) performing a 6‐wk high‐intensity interval training intervention using a repeated measures/cross‐over study design. Venous blood samples were collected from each subject at three time intervals (baseline, postexercise, recovery) in their naïve and trained states while completing standardized cycling trials at the same absolute workload. Complementary statistical methods were used to classify dynamic changes in plasma metabolism associated with strenuous exercise and training status. Positive adaptations to exercise were associated with training‐induced upregulation in plasma l ‐carnitine at rest due to improved muscle oxidative capacity, and greater antioxidant capacity as reflected by lower circulating glutathionyl‐ l ‐cysteine mixed disulfide. Attenuation in plasma hypoxanthine and higher O ‐acetyl‐ l ‐carnitine levels postexercise also indicated lower energetic stress for trained women. Nutzungsrecht: © 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim © COPYRIGHT 2015 Wiley Subscription Services, Inc. Mass spectrometry Exercise training Capillary electrophoresis Biomarker discovery Metabolomics Antioxidants Cysteine Training Analysis Physiological aspects Levocarnitine Metabolites Purines Working women Gillen, Jenna B oth Kormendi, Aleshia oth Lam, Karen P oth DiBattista, Alicia oth Gibala, Martin J oth Britz‐McKibbin, Philip oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 36(2015), 18, Seite 2226-2236 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:36 year:2015 number:18 pages:2226-2236 http://dx.doi.org/10.1002/elps.201400604 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201400604/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 36 2015 18 2226-2236
language	English
source	Enthalten in Electrophoresis 36(2015), 18, Seite 2226-2236 volume:36 year:2015 number:18 pages:2226-2236
sourceStr	Enthalten in Electrophoresis 36(2015), 18, Seite 2226-2236 volume:36 year:2015 number:18 pages:2226-2236
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Mass spectrometry Exercise training Capillary electrophoresis Biomarker discovery Metabolomics Antioxidants Cysteine Training Analysis Physiological aspects Levocarnitine Metabolites Purines Working women
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author	Kuehnbaum, Naomi L
spellingShingle	Kuehnbaum, Naomi L ddc 540 ddc 570 fid BIODIV bkl 35.29 misc Mass spectrometry misc Exercise training misc Capillary electrophoresis misc Biomarker discovery misc Metabolomics misc Antioxidants misc Cysteine misc Training misc Analysis misc Physiological aspects misc Levocarnitine misc Metabolites misc Purines misc Working women Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training
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topic_title	540 570 DNB 570 AVZ BIODIV fid 35.29 bkl Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training Mass spectrometry Exercise training Capillary electrophoresis Biomarker discovery Metabolomics Antioxidants Cysteine Training Analysis Physiological aspects Levocarnitine Metabolites Purines Working women
topic	ddc 540 ddc 570 fid BIODIV bkl 35.29 misc Mass spectrometry misc Exercise training misc Capillary electrophoresis misc Biomarker discovery misc Metabolomics misc Antioxidants misc Cysteine misc Training misc Analysis misc Physiological aspects misc Levocarnitine misc Metabolites misc Purines misc Working women
topic_unstemmed	ddc 540 ddc 570 fid BIODIV bkl 35.29 misc Mass spectrometry misc Exercise training misc Capillary electrophoresis misc Biomarker discovery misc Metabolomics misc Antioxidants misc Cysteine misc Training misc Analysis misc Physiological aspects misc Levocarnitine misc Metabolites misc Purines misc Working women
topic_browse	ddc 540 ddc 570 fid BIODIV bkl 35.29 misc Mass spectrometry misc Exercise training misc Capillary electrophoresis misc Biomarker discovery misc Metabolomics misc Antioxidants misc Cysteine misc Training misc Analysis misc Physiological aspects misc Levocarnitine misc Metabolites misc Purines misc Working women
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title	Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training
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title_full	Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training
author_sort	Kuehnbaum, Naomi L
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title_sort	multiplexed separations for biomarker discovery in metabolomics: elucidating adaptive responses to exercise training
title_auth	Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training
abstract	High efficiency separations are needed to enhance selectivity, mass spectral quality, and quantitative performance in metabolomic studies. However, low sample throughput and complicated data preprocessing remain major bottlenecks to biomarker discovery. We introduce an accelerated data workflow to identify plasma metabolite signatures of exercise responsiveness when using multisegment injection‐capillary electrophoresis‐mass spectrometry (MSI‐CE‐MS). This multiplexed separation platform takes advantage of customizable serial injections to enhance sample throughput and data fidelity based on temporally resolved ion signals derived from seven different sample segments analyzed within a single run. MSI‐CE‐MS was applied to explore the adaptive metabolic responses of a cohort of overweight/obese women ( BMI > 25, n = 9) performing a 6‐wk high‐intensity interval training intervention using a repeated measures/cross‐over study design. Venous blood samples were collected from each subject at three time intervals (baseline, postexercise, recovery) in their naïve and trained states while completing standardized cycling trials at the same absolute workload. Complementary statistical methods were used to classify dynamic changes in plasma metabolism associated with strenuous exercise and training status. Positive adaptations to exercise were associated with training‐induced upregulation in plasma l ‐carnitine at rest due to improved muscle oxidative capacity, and greater antioxidant capacity as reflected by lower circulating glutathionyl‐ l ‐cysteine mixed disulfide. Attenuation in plasma hypoxanthine and higher O ‐acetyl‐ l ‐carnitine levels postexercise also indicated lower energetic stress for trained women.
abstractGer	High efficiency separations are needed to enhance selectivity, mass spectral quality, and quantitative performance in metabolomic studies. However, low sample throughput and complicated data preprocessing remain major bottlenecks to biomarker discovery. We introduce an accelerated data workflow to identify plasma metabolite signatures of exercise responsiveness when using multisegment injection‐capillary electrophoresis‐mass spectrometry (MSI‐CE‐MS). This multiplexed separation platform takes advantage of customizable serial injections to enhance sample throughput and data fidelity based on temporally resolved ion signals derived from seven different sample segments analyzed within a single run. MSI‐CE‐MS was applied to explore the adaptive metabolic responses of a cohort of overweight/obese women ( BMI > 25, n = 9) performing a 6‐wk high‐intensity interval training intervention using a repeated measures/cross‐over study design. Venous blood samples were collected from each subject at three time intervals (baseline, postexercise, recovery) in their naïve and trained states while completing standardized cycling trials at the same absolute workload. Complementary statistical methods were used to classify dynamic changes in plasma metabolism associated with strenuous exercise and training status. Positive adaptations to exercise were associated with training‐induced upregulation in plasma l ‐carnitine at rest due to improved muscle oxidative capacity, and greater antioxidant capacity as reflected by lower circulating glutathionyl‐ l ‐cysteine mixed disulfide. Attenuation in plasma hypoxanthine and higher O ‐acetyl‐ l ‐carnitine levels postexercise also indicated lower energetic stress for trained women.
abstract_unstemmed	High efficiency separations are needed to enhance selectivity, mass spectral quality, and quantitative performance in metabolomic studies. However, low sample throughput and complicated data preprocessing remain major bottlenecks to biomarker discovery. We introduce an accelerated data workflow to identify plasma metabolite signatures of exercise responsiveness when using multisegment injection‐capillary electrophoresis‐mass spectrometry (MSI‐CE‐MS). This multiplexed separation platform takes advantage of customizable serial injections to enhance sample throughput and data fidelity based on temporally resolved ion signals derived from seven different sample segments analyzed within a single run. MSI‐CE‐MS was applied to explore the adaptive metabolic responses of a cohort of overweight/obese women ( BMI > 25, n = 9) performing a 6‐wk high‐intensity interval training intervention using a repeated measures/cross‐over study design. Venous blood samples were collected from each subject at three time intervals (baseline, postexercise, recovery) in their naïve and trained states while completing standardized cycling trials at the same absolute workload. Complementary statistical methods were used to classify dynamic changes in plasma metabolism associated with strenuous exercise and training status. Positive adaptations to exercise were associated with training‐induced upregulation in plasma l ‐carnitine at rest due to improved muscle oxidative capacity, and greater antioxidant capacity as reflected by lower circulating glutathionyl‐ l ‐cysteine mixed disulfide. Attenuation in plasma hypoxanthine and higher O ‐acetyl‐ l ‐carnitine levels postexercise also indicated lower energetic stress for trained women.
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title_short	Multiplexed separations for biomarker discovery in metabolomics: Elucidating adaptive responses to exercise training
url	http://dx.doi.org/10.1002/elps.201400604 http://onlinelibrary.wiley.com/doi/10.1002/elps.201400604/abstract
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author2	Gillen, Jenna B Kormendi, Aleshia Lam, Karen P DiBattista, Alicia Gibala, Martin J Britz‐McKibbin, Philip
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up_date	2024-07-03T15:16:59.049Z
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