Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines

Previously, we have shown that a combination between X-irradiation and low-intensity pulsed ultrasound (US) could synergistically suppress cell survival post exposure (Buldakov et al., 2014). In this study, the cellular effects underlying the enhanced cell killing are investigated. U937 and Molt-4 c...
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Autor*in:	Buldakov, Mikhail A [verfasserIn] Hassan, Mariame A Jawaid, Paras Cherdyntseva, Nadejda V Kondo, Takashi

Format:	Artikel
Sprache:	Englisch

Erschienen:	2015

Rechteinformationen:	Nutzungsrecht: Copyright © 2014. Published by Elsevier B.V.

Schlagwörter:	Cell Death - radiation effects Leukemia - pathology Cell Survival - radiation effects Cell Cycle - radiation effects

Übergeordnetes Werk:	Enthalten in: Ultrasonics sonochemistry - Amsterdam [u.a.] : Elsevier, 1994, 23(2015), Seite 339-346
Übergeordnetes Werk:	volume:23 ; year:2015 ; pages:339-346

Links:	Volltext Link aufrufen

DOI / URN:	10.1016/j.ultsonch.2014.08.018

Katalog-ID:	OLC1959812467

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spelling	10.1016/j.ultsonch.2014.08.018 doi PQ20160617 (DE-627)OLC1959812467 (DE-599)GBVOLC1959812467 (PRQ)c1544-849e19b03ce9d49e4988d66e82997c0652e0fe5849f4740f14cb0fb7adfaacc80 (KEY)0238159120150000023000000339cellulareffectsoflowintensitypulsedultrasoundandxi DE-627 ger DE-627 rakwb eng 540 DE-600 Buldakov, Mikhail A verfasserin aut Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Previously, we have shown that a combination between X-irradiation and low-intensity pulsed ultrasound (US) could synergistically suppress cell survival post exposure (Buldakov et al., 2014). In this study, the cellular effects underlying the enhanced cell killing are investigated. U937 and Molt-4 cell lines were exposed to 1.0 MHz US with 50% duty factor at 0.3 W/cm(2) and pulsed at 1, 5 and 10 Hz immediately after exposure to X-rays at 0, 0.5, 2.5 and 5 Gy. The cells were assayed at different time points to depict the major cellular events that culminated in cell death. For instance, membrane damage and cell lysis were estimated immediately following exposure and 24 h later. Intracellular reactive oxygen species (ROS) were also determined flow cytometrically after treatment. Moreover, the extent of DNA damage and cell cycle progression were determined at 6 and 24 h, respectively. Despite the general trend for synergism, there was a disproportionation of mediating factors depending on the cell type and its specific biological makeup. Immediately, US could induce appreciable necrotic cell death through extensive membrane damage in U937 but induced cell lysis in Molt-4 cells. ROS might have contributed to cell killing in Molt-4 but not in U937 cells. Although both of the physical modalities are significantly DNA-damaging alone, no additional damage was observed in combination. Moreover, override in some arrested cell cycle phases was also observed following combination. Collectively, the interaction between X-rays and US seems to depend mainly on the acoustic environment determined by the setup and this might explain the contradictory data among reports. Nutzungsrecht: Copyright © 2014. Published by Elsevier B.V. Cell Death - radiation effects Leukemia - pathology Cell Survival - radiation effects Cell Cycle - radiation effects Hassan, Mariame A oth Jawaid, Paras oth Cherdyntseva, Nadejda V oth Kondo, Takashi oth Enthalten in Ultrasonics sonochemistry Amsterdam [u.a.] : Elsevier, 1994 23(2015), Seite 339-346 (DE-627)182456803 (DE-600)1208333-1 (DE-576)043089054 1350-4177 nnns volume:23 year:2015 pages:339-346 http://dx.doi.org/10.1016/j.ultsonch.2014.08.018 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25287395 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_170 GBV_ILN_2006 GBV_ILN_4012 AR 23 2015 339-346
allfields_unstemmed	10.1016/j.ultsonch.2014.08.018 doi PQ20160617 (DE-627)OLC1959812467 (DE-599)GBVOLC1959812467 (PRQ)c1544-849e19b03ce9d49e4988d66e82997c0652e0fe5849f4740f14cb0fb7adfaacc80 (KEY)0238159120150000023000000339cellulareffectsoflowintensitypulsedultrasoundandxi DE-627 ger DE-627 rakwb eng 540 DE-600 Buldakov, Mikhail A verfasserin aut Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Previously, we have shown that a combination between X-irradiation and low-intensity pulsed ultrasound (US) could synergistically suppress cell survival post exposure (Buldakov et al., 2014). In this study, the cellular effects underlying the enhanced cell killing are investigated. U937 and Molt-4 cell lines were exposed to 1.0 MHz US with 50% duty factor at 0.3 W/cm(2) and pulsed at 1, 5 and 10 Hz immediately after exposure to X-rays at 0, 0.5, 2.5 and 5 Gy. The cells were assayed at different time points to depict the major cellular events that culminated in cell death. For instance, membrane damage and cell lysis were estimated immediately following exposure and 24 h later. Intracellular reactive oxygen species (ROS) were also determined flow cytometrically after treatment. Moreover, the extent of DNA damage and cell cycle progression were determined at 6 and 24 h, respectively. Despite the general trend for synergism, there was a disproportionation of mediating factors depending on the cell type and its specific biological makeup. Immediately, US could induce appreciable necrotic cell death through extensive membrane damage in U937 but induced cell lysis in Molt-4 cells. ROS might have contributed to cell killing in Molt-4 but not in U937 cells. Although both of the physical modalities are significantly DNA-damaging alone, no additional damage was observed in combination. Moreover, override in some arrested cell cycle phases was also observed following combination. Collectively, the interaction between X-rays and US seems to depend mainly on the acoustic environment determined by the setup and this might explain the contradictory data among reports. Nutzungsrecht: Copyright © 2014. Published by Elsevier B.V. Cell Death - radiation effects Leukemia - pathology Cell Survival - radiation effects Cell Cycle - radiation effects Hassan, Mariame A oth Jawaid, Paras oth Cherdyntseva, Nadejda V oth Kondo, Takashi oth Enthalten in Ultrasonics sonochemistry Amsterdam [u.a.] : Elsevier, 1994 23(2015), Seite 339-346 (DE-627)182456803 (DE-600)1208333-1 (DE-576)043089054 1350-4177 nnns volume:23 year:2015 pages:339-346 http://dx.doi.org/10.1016/j.ultsonch.2014.08.018 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25287395 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_170 GBV_ILN_2006 GBV_ILN_4012 AR 23 2015 339-346
allfieldsGer	10.1016/j.ultsonch.2014.08.018 doi PQ20160617 (DE-627)OLC1959812467 (DE-599)GBVOLC1959812467 (PRQ)c1544-849e19b03ce9d49e4988d66e82997c0652e0fe5849f4740f14cb0fb7adfaacc80 (KEY)0238159120150000023000000339cellulareffectsoflowintensitypulsedultrasoundandxi DE-627 ger DE-627 rakwb eng 540 DE-600 Buldakov, Mikhail A verfasserin aut Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Previously, we have shown that a combination between X-irradiation and low-intensity pulsed ultrasound (US) could synergistically suppress cell survival post exposure (Buldakov et al., 2014). In this study, the cellular effects underlying the enhanced cell killing are investigated. U937 and Molt-4 cell lines were exposed to 1.0 MHz US with 50% duty factor at 0.3 W/cm(2) and pulsed at 1, 5 and 10 Hz immediately after exposure to X-rays at 0, 0.5, 2.5 and 5 Gy. The cells were assayed at different time points to depict the major cellular events that culminated in cell death. For instance, membrane damage and cell lysis were estimated immediately following exposure and 24 h later. Intracellular reactive oxygen species (ROS) were also determined flow cytometrically after treatment. Moreover, the extent of DNA damage and cell cycle progression were determined at 6 and 24 h, respectively. Despite the general trend for synergism, there was a disproportionation of mediating factors depending on the cell type and its specific biological makeup. Immediately, US could induce appreciable necrotic cell death through extensive membrane damage in U937 but induced cell lysis in Molt-4 cells. ROS might have contributed to cell killing in Molt-4 but not in U937 cells. Although both of the physical modalities are significantly DNA-damaging alone, no additional damage was observed in combination. Moreover, override in some arrested cell cycle phases was also observed following combination. Collectively, the interaction between X-rays and US seems to depend mainly on the acoustic environment determined by the setup and this might explain the contradictory data among reports. Nutzungsrecht: Copyright © 2014. Published by Elsevier B.V. Cell Death - radiation effects Leukemia - pathology Cell Survival - radiation effects Cell Cycle - radiation effects Hassan, Mariame A oth Jawaid, Paras oth Cherdyntseva, Nadejda V oth Kondo, Takashi oth Enthalten in Ultrasonics sonochemistry Amsterdam [u.a.] : Elsevier, 1994 23(2015), Seite 339-346 (DE-627)182456803 (DE-600)1208333-1 (DE-576)043089054 1350-4177 nnns volume:23 year:2015 pages:339-346 http://dx.doi.org/10.1016/j.ultsonch.2014.08.018 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25287395 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_170 GBV_ILN_2006 GBV_ILN_4012 AR 23 2015 339-346
allfieldsSound	10.1016/j.ultsonch.2014.08.018 doi PQ20160617 (DE-627)OLC1959812467 (DE-599)GBVOLC1959812467 (PRQ)c1544-849e19b03ce9d49e4988d66e82997c0652e0fe5849f4740f14cb0fb7adfaacc80 (KEY)0238159120150000023000000339cellulareffectsoflowintensitypulsedultrasoundandxi DE-627 ger DE-627 rakwb eng 540 DE-600 Buldakov, Mikhail A verfasserin aut Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Previously, we have shown that a combination between X-irradiation and low-intensity pulsed ultrasound (US) could synergistically suppress cell survival post exposure (Buldakov et al., 2014). In this study, the cellular effects underlying the enhanced cell killing are investigated. U937 and Molt-4 cell lines were exposed to 1.0 MHz US with 50% duty factor at 0.3 W/cm(2) and pulsed at 1, 5 and 10 Hz immediately after exposure to X-rays at 0, 0.5, 2.5 and 5 Gy. The cells were assayed at different time points to depict the major cellular events that culminated in cell death. For instance, membrane damage and cell lysis were estimated immediately following exposure and 24 h later. Intracellular reactive oxygen species (ROS) were also determined flow cytometrically after treatment. Moreover, the extent of DNA damage and cell cycle progression were determined at 6 and 24 h, respectively. Despite the general trend for synergism, there was a disproportionation of mediating factors depending on the cell type and its specific biological makeup. Immediately, US could induce appreciable necrotic cell death through extensive membrane damage in U937 but induced cell lysis in Molt-4 cells. ROS might have contributed to cell killing in Molt-4 but not in U937 cells. Although both of the physical modalities are significantly DNA-damaging alone, no additional damage was observed in combination. Moreover, override in some arrested cell cycle phases was also observed following combination. Collectively, the interaction between X-rays and US seems to depend mainly on the acoustic environment determined by the setup and this might explain the contradictory data among reports. Nutzungsrecht: Copyright © 2014. Published by Elsevier B.V. Cell Death - radiation effects Leukemia - pathology Cell Survival - radiation effects Cell Cycle - radiation effects Hassan, Mariame A oth Jawaid, Paras oth Cherdyntseva, Nadejda V oth Kondo, Takashi oth Enthalten in Ultrasonics sonochemistry Amsterdam [u.a.] : Elsevier, 1994 23(2015), Seite 339-346 (DE-627)182456803 (DE-600)1208333-1 (DE-576)043089054 1350-4177 nnns volume:23 year:2015 pages:339-346 http://dx.doi.org/10.1016/j.ultsonch.2014.08.018 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25287395 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_170 GBV_ILN_2006 GBV_ILN_4012 AR 23 2015 339-346
language	English
source	Enthalten in Ultrasonics sonochemistry 23(2015), Seite 339-346 volume:23 year:2015 pages:339-346
sourceStr	Enthalten in Ultrasonics sonochemistry 23(2015), Seite 339-346 volume:23 year:2015 pages:339-346
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topic_facet	Cell Death - radiation effects Leukemia - pathology Cell Survival - radiation effects Cell Cycle - radiation effects
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author	Buldakov, Mikhail A
spellingShingle	Buldakov, Mikhail A ddc 540 misc Cell Death - radiation effects misc Leukemia - pathology misc Cell Survival - radiation effects misc Cell Cycle - radiation effects Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines
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topic_title	540 DE-600 Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines Cell Death - radiation effects Leukemia - pathology Cell Survival - radiation effects Cell Cycle - radiation effects
topic	ddc 540 misc Cell Death - radiation effects misc Leukemia - pathology misc Cell Survival - radiation effects misc Cell Cycle - radiation effects
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title	Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines
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title_full	Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines
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title_sort	cellular effects of low-intensity pulsed ultrasound and x-irradiation in combination in two human leukaemia cell lines
title_auth	Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines
abstract	Previously, we have shown that a combination between X-irradiation and low-intensity pulsed ultrasound (US) could synergistically suppress cell survival post exposure (Buldakov et al., 2014). In this study, the cellular effects underlying the enhanced cell killing are investigated. U937 and Molt-4 cell lines were exposed to 1.0 MHz US with 50% duty factor at 0.3 W/cm(2) and pulsed at 1, 5 and 10 Hz immediately after exposure to X-rays at 0, 0.5, 2.5 and 5 Gy. The cells were assayed at different time points to depict the major cellular events that culminated in cell death. For instance, membrane damage and cell lysis were estimated immediately following exposure and 24 h later. Intracellular reactive oxygen species (ROS) were also determined flow cytometrically after treatment. Moreover, the extent of DNA damage and cell cycle progression were determined at 6 and 24 h, respectively. Despite the general trend for synergism, there was a disproportionation of mediating factors depending on the cell type and its specific biological makeup. Immediately, US could induce appreciable necrotic cell death through extensive membrane damage in U937 but induced cell lysis in Molt-4 cells. ROS might have contributed to cell killing in Molt-4 but not in U937 cells. Although both of the physical modalities are significantly DNA-damaging alone, no additional damage was observed in combination. Moreover, override in some arrested cell cycle phases was also observed following combination. Collectively, the interaction between X-rays and US seems to depend mainly on the acoustic environment determined by the setup and this might explain the contradictory data among reports.
abstractGer	Previously, we have shown that a combination between X-irradiation and low-intensity pulsed ultrasound (US) could synergistically suppress cell survival post exposure (Buldakov et al., 2014). In this study, the cellular effects underlying the enhanced cell killing are investigated. U937 and Molt-4 cell lines were exposed to 1.0 MHz US with 50% duty factor at 0.3 W/cm(2) and pulsed at 1, 5 and 10 Hz immediately after exposure to X-rays at 0, 0.5, 2.5 and 5 Gy. The cells were assayed at different time points to depict the major cellular events that culminated in cell death. For instance, membrane damage and cell lysis were estimated immediately following exposure and 24 h later. Intracellular reactive oxygen species (ROS) were also determined flow cytometrically after treatment. Moreover, the extent of DNA damage and cell cycle progression were determined at 6 and 24 h, respectively. Despite the general trend for synergism, there was a disproportionation of mediating factors depending on the cell type and its specific biological makeup. Immediately, US could induce appreciable necrotic cell death through extensive membrane damage in U937 but induced cell lysis in Molt-4 cells. ROS might have contributed to cell killing in Molt-4 but not in U937 cells. Although both of the physical modalities are significantly DNA-damaging alone, no additional damage was observed in combination. Moreover, override in some arrested cell cycle phases was also observed following combination. Collectively, the interaction between X-rays and US seems to depend mainly on the acoustic environment determined by the setup and this might explain the contradictory data among reports.
abstract_unstemmed	Previously, we have shown that a combination between X-irradiation and low-intensity pulsed ultrasound (US) could synergistically suppress cell survival post exposure (Buldakov et al., 2014). In this study, the cellular effects underlying the enhanced cell killing are investigated. U937 and Molt-4 cell lines were exposed to 1.0 MHz US with 50% duty factor at 0.3 W/cm(2) and pulsed at 1, 5 and 10 Hz immediately after exposure to X-rays at 0, 0.5, 2.5 and 5 Gy. The cells were assayed at different time points to depict the major cellular events that culminated in cell death. For instance, membrane damage and cell lysis were estimated immediately following exposure and 24 h later. Intracellular reactive oxygen species (ROS) were also determined flow cytometrically after treatment. Moreover, the extent of DNA damage and cell cycle progression were determined at 6 and 24 h, respectively. Despite the general trend for synergism, there was a disproportionation of mediating factors depending on the cell type and its specific biological makeup. Immediately, US could induce appreciable necrotic cell death through extensive membrane damage in U937 but induced cell lysis in Molt-4 cells. ROS might have contributed to cell killing in Molt-4 but not in U937 cells. Although both of the physical modalities are significantly DNA-damaging alone, no additional damage was observed in combination. Moreover, override in some arrested cell cycle phases was also observed following combination. Collectively, the interaction between X-rays and US seems to depend mainly on the acoustic environment determined by the setup and this might explain the contradictory data among reports.
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title_short	Cellular effects of low-intensity pulsed ultrasound and X-irradiation in combination in two human leukaemia cell lines
url	http://dx.doi.org/10.1016/j.ultsonch.2014.08.018 http://www.ncbi.nlm.nih.gov/pubmed/25287395
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up_date	2024-07-03T18:52:54.914Z
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