Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana
Chlorogenic acid and its derivatives (CADs) are valuable bioactive plant secondary metabolites with many health benefits. In the present study, Stevia rebaudiana hairy root cultures were established, and the culture conditions for the production of CADs were optimized. The hairy roots were induced b...
Ausführliche Beschreibung
Autor*in: |
Fu, Xiao [verfasserIn] |
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Artikel |
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Englisch |
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2015 |
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Übergeordnetes Werk: |
Enthalten in: Journal of agricultural and food chemistry - Columbus, Ohio : American Chemical Soc., 1953, 63(2015), 1, Seite 262 |
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Übergeordnetes Werk: |
volume:63 ; year:2015 ; number:1 ; pages:262 |
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520 | |a Chlorogenic acid and its derivatives (CADs) are valuable bioactive plant secondary metabolites with many health benefits. In the present study, Stevia rebaudiana hairy root cultures were established, and the culture conditions for the production of CADs were optimized. The hairy roots were induced by coculture of S. rebaudiana leaves and Agrobacterium rhizogenes (C58C1) after infection, which were further verified by PCR detection of rolB and rolC genes. HPLC-MS and HPLC analysis showed that chlorogenic acid (3-caffeoylquinic acid, 3-CQA), 3,5-dicaffeoylquinic acid (3,5-CQA), and 4,5-dicaffeoylquinic acid (4,5-CQA) were the major CADs in the hairy roots. Eight single roots with rapid growth rate were selected. Among them, T3 had the highest yield of CADs. B5 medium supplemented with 40 g/L sucrose was more suitable for the production of CADs than others. Under optimal culture conditions, the total content of these three compounds reached 105.58 mg/g and total yield was 234.40 mg/100 mL. | ||
650 | 4 | |a Plant Roots - chemistry | |
650 | 4 | |a Stevia - chemistry | |
650 | 4 | |a Chlorogenic Acid - chemistry | |
650 | 4 | |a Plant Extracts - metabolism | |
650 | 4 | |a Plant Roots - metabolism | |
650 | 4 | |a Culture Media - chemistry | |
650 | 4 | |a Stevia - growth & development | |
650 | 4 | |a Stevia - metabolism | |
650 | 4 | |a Plant Extracts - chemistry | |
650 | 4 | |a Culture Media - metabolism | |
650 | 4 | |a Plant Roots - growth & development | |
650 | 4 | |a Chlorogenic Acid - metabolism | |
700 | 1 | |a Yin, Zhong-Ping |4 oth | |
700 | 1 | |a Chen, Ji-Guang |4 oth | |
700 | 1 | |a Shangguan, Xin-Chen |4 oth | |
700 | 1 | |a Wang, Xiaoqiang |4 oth | |
700 | 1 | |a Zhang, Qing-Feng |4 oth | |
700 | 1 | |a Peng, Da-Yong |4 oth | |
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PQ20160617 (DE-627)OLC1963007263 (DE-599)GBVOLC1963007263 (PRQ)p476-6a7286cd41c667716888c3756afc626b7979f7a7c5a0c822e0336fd8975e70650 (KEY)0011841920150000063000100262productionofchlorogenicacidanditsderivativesinhair DE-627 ger DE-627 rakwb eng 630 640 540 DNB 58.27 bkl 58.34 bkl 42.63 bkl Fu, Xiao verfasserin aut Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Chlorogenic acid and its derivatives (CADs) are valuable bioactive plant secondary metabolites with many health benefits. In the present study, Stevia rebaudiana hairy root cultures were established, and the culture conditions for the production of CADs were optimized. The hairy roots were induced by coculture of S. rebaudiana leaves and Agrobacterium rhizogenes (C58C1) after infection, which were further verified by PCR detection of rolB and rolC genes. HPLC-MS and HPLC analysis showed that chlorogenic acid (3-caffeoylquinic acid, 3-CQA), 3,5-dicaffeoylquinic acid (3,5-CQA), and 4,5-dicaffeoylquinic acid (4,5-CQA) were the major CADs in the hairy roots. Eight single roots with rapid growth rate were selected. Among them, T3 had the highest yield of CADs. B5 medium supplemented with 40 g/L sucrose was more suitable for the production of CADs than others. Under optimal culture conditions, the total content of these three compounds reached 105.58 mg/g and total yield was 234.40 mg/100 mL. Plant Roots - chemistry Stevia - chemistry Chlorogenic Acid - chemistry Plant Extracts - metabolism Plant Roots - metabolism Culture Media - chemistry Stevia - growth & development Stevia - metabolism Plant Extracts - chemistry Culture Media - metabolism Plant Roots - growth & development Chlorogenic Acid - metabolism Yin, Zhong-Ping oth Chen, Ji-Guang oth Shangguan, Xin-Chen oth Wang, Xiaoqiang oth Zhang, Qing-Feng oth Peng, Da-Yong oth Enthalten in Journal of agricultural and food chemistry Columbus, Ohio : American Chemical Soc., 1953 63(2015), 1, Seite 262 (DE-627)129602795 (DE-600)241619-0 (DE-576)015096610 0021-8561 nnns volume:63 year:2015 number:1 pages:262 http://www.ncbi.nlm.nih.gov/pubmed/25548875 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_2006 GBV_ILN_4219 58.27 AVZ 58.34 AVZ 42.63 AVZ AR 63 2015 1 262 |
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PQ20160617 (DE-627)OLC1963007263 (DE-599)GBVOLC1963007263 (PRQ)p476-6a7286cd41c667716888c3756afc626b7979f7a7c5a0c822e0336fd8975e70650 (KEY)0011841920150000063000100262productionofchlorogenicacidanditsderivativesinhair DE-627 ger DE-627 rakwb eng 630 640 540 DNB 58.27 bkl 58.34 bkl 42.63 bkl Fu, Xiao verfasserin aut Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Chlorogenic acid and its derivatives (CADs) are valuable bioactive plant secondary metabolites with many health benefits. In the present study, Stevia rebaudiana hairy root cultures were established, and the culture conditions for the production of CADs were optimized. The hairy roots were induced by coculture of S. rebaudiana leaves and Agrobacterium rhizogenes (C58C1) after infection, which were further verified by PCR detection of rolB and rolC genes. HPLC-MS and HPLC analysis showed that chlorogenic acid (3-caffeoylquinic acid, 3-CQA), 3,5-dicaffeoylquinic acid (3,5-CQA), and 4,5-dicaffeoylquinic acid (4,5-CQA) were the major CADs in the hairy roots. Eight single roots with rapid growth rate were selected. Among them, T3 had the highest yield of CADs. B5 medium supplemented with 40 g/L sucrose was more suitable for the production of CADs than others. Under optimal culture conditions, the total content of these three compounds reached 105.58 mg/g and total yield was 234.40 mg/100 mL. Plant Roots - chemistry Stevia - chemistry Chlorogenic Acid - chemistry Plant Extracts - metabolism Plant Roots - metabolism Culture Media - chemistry Stevia - growth & development Stevia - metabolism Plant Extracts - chemistry Culture Media - metabolism Plant Roots - growth & development Chlorogenic Acid - metabolism Yin, Zhong-Ping oth Chen, Ji-Guang oth Shangguan, Xin-Chen oth Wang, Xiaoqiang oth Zhang, Qing-Feng oth Peng, Da-Yong oth Enthalten in Journal of agricultural and food chemistry Columbus, Ohio : American Chemical Soc., 1953 63(2015), 1, Seite 262 (DE-627)129602795 (DE-600)241619-0 (DE-576)015096610 0021-8561 nnns volume:63 year:2015 number:1 pages:262 http://www.ncbi.nlm.nih.gov/pubmed/25548875 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_2006 GBV_ILN_4219 58.27 AVZ 58.34 AVZ 42.63 AVZ AR 63 2015 1 262 |
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PQ20160617 (DE-627)OLC1963007263 (DE-599)GBVOLC1963007263 (PRQ)p476-6a7286cd41c667716888c3756afc626b7979f7a7c5a0c822e0336fd8975e70650 (KEY)0011841920150000063000100262productionofchlorogenicacidanditsderivativesinhair DE-627 ger DE-627 rakwb eng 630 640 540 DNB 58.27 bkl 58.34 bkl 42.63 bkl Fu, Xiao verfasserin aut Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Chlorogenic acid and its derivatives (CADs) are valuable bioactive plant secondary metabolites with many health benefits. In the present study, Stevia rebaudiana hairy root cultures were established, and the culture conditions for the production of CADs were optimized. The hairy roots were induced by coculture of S. rebaudiana leaves and Agrobacterium rhizogenes (C58C1) after infection, which were further verified by PCR detection of rolB and rolC genes. HPLC-MS and HPLC analysis showed that chlorogenic acid (3-caffeoylquinic acid, 3-CQA), 3,5-dicaffeoylquinic acid (3,5-CQA), and 4,5-dicaffeoylquinic acid (4,5-CQA) were the major CADs in the hairy roots. Eight single roots with rapid growth rate were selected. Among them, T3 had the highest yield of CADs. B5 medium supplemented with 40 g/L sucrose was more suitable for the production of CADs than others. Under optimal culture conditions, the total content of these three compounds reached 105.58 mg/g and total yield was 234.40 mg/100 mL. Plant Roots - chemistry Stevia - chemistry Chlorogenic Acid - chemistry Plant Extracts - metabolism Plant Roots - metabolism Culture Media - chemistry Stevia - growth & development Stevia - metabolism Plant Extracts - chemistry Culture Media - metabolism Plant Roots - growth & development Chlorogenic Acid - metabolism Yin, Zhong-Ping oth Chen, Ji-Guang oth Shangguan, Xin-Chen oth Wang, Xiaoqiang oth Zhang, Qing-Feng oth Peng, Da-Yong oth Enthalten in Journal of agricultural and food chemistry Columbus, Ohio : American Chemical Soc., 1953 63(2015), 1, Seite 262 (DE-627)129602795 (DE-600)241619-0 (DE-576)015096610 0021-8561 nnns volume:63 year:2015 number:1 pages:262 http://www.ncbi.nlm.nih.gov/pubmed/25548875 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_2006 GBV_ILN_4219 58.27 AVZ 58.34 AVZ 42.63 AVZ AR 63 2015 1 262 |
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Plant Roots - chemistry Stevia - chemistry Chlorogenic Acid - chemistry Plant Extracts - metabolism Plant Roots - metabolism Culture Media - chemistry Stevia - growth & development Stevia - metabolism Plant Extracts - chemistry Culture Media - metabolism Plant Roots - growth & development Chlorogenic Acid - metabolism |
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Fu, Xiao |
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Fu, Xiao ddc 630 bkl 58.27 bkl 58.34 bkl 42.63 misc Plant Roots - chemistry misc Stevia - chemistry misc Chlorogenic Acid - chemistry misc Plant Extracts - metabolism misc Plant Roots - metabolism misc Culture Media - chemistry misc Stevia - growth & development misc Stevia - metabolism misc Plant Extracts - chemistry misc Culture Media - metabolism misc Plant Roots - growth & development misc Chlorogenic Acid - metabolism Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana |
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630 640 540 DNB 58.27 bkl 58.34 bkl 42.63 bkl Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana Plant Roots - chemistry Stevia - chemistry Chlorogenic Acid - chemistry Plant Extracts - metabolism Plant Roots - metabolism Culture Media - chemistry Stevia - growth & development Stevia - metabolism Plant Extracts - chemistry Culture Media - metabolism Plant Roots - growth & development Chlorogenic Acid - metabolism |
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ddc 630 bkl 58.27 bkl 58.34 bkl 42.63 misc Plant Roots - chemistry misc Stevia - chemistry misc Chlorogenic Acid - chemistry misc Plant Extracts - metabolism misc Plant Roots - metabolism misc Culture Media - chemistry misc Stevia - growth & development misc Stevia - metabolism misc Plant Extracts - chemistry misc Culture Media - metabolism misc Plant Roots - growth & development misc Chlorogenic Acid - metabolism |
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ddc 630 bkl 58.27 bkl 58.34 bkl 42.63 misc Plant Roots - chemistry misc Stevia - chemistry misc Chlorogenic Acid - chemistry misc Plant Extracts - metabolism misc Plant Roots - metabolism misc Culture Media - chemistry misc Stevia - growth & development misc Stevia - metabolism misc Plant Extracts - chemistry misc Culture Media - metabolism misc Plant Roots - growth & development misc Chlorogenic Acid - metabolism |
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Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana |
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Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana |
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production of chlorogenic acid and its derivatives in hairy root cultures of stevia rebaudiana |
title_auth |
Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana |
abstract |
Chlorogenic acid and its derivatives (CADs) are valuable bioactive plant secondary metabolites with many health benefits. In the present study, Stevia rebaudiana hairy root cultures were established, and the culture conditions for the production of CADs were optimized. The hairy roots were induced by coculture of S. rebaudiana leaves and Agrobacterium rhizogenes (C58C1) after infection, which were further verified by PCR detection of rolB and rolC genes. HPLC-MS and HPLC analysis showed that chlorogenic acid (3-caffeoylquinic acid, 3-CQA), 3,5-dicaffeoylquinic acid (3,5-CQA), and 4,5-dicaffeoylquinic acid (4,5-CQA) were the major CADs in the hairy roots. Eight single roots with rapid growth rate were selected. Among them, T3 had the highest yield of CADs. B5 medium supplemented with 40 g/L sucrose was more suitable for the production of CADs than others. Under optimal culture conditions, the total content of these three compounds reached 105.58 mg/g and total yield was 234.40 mg/100 mL. |
abstractGer |
Chlorogenic acid and its derivatives (CADs) are valuable bioactive plant secondary metabolites with many health benefits. In the present study, Stevia rebaudiana hairy root cultures were established, and the culture conditions for the production of CADs were optimized. The hairy roots were induced by coculture of S. rebaudiana leaves and Agrobacterium rhizogenes (C58C1) after infection, which were further verified by PCR detection of rolB and rolC genes. HPLC-MS and HPLC analysis showed that chlorogenic acid (3-caffeoylquinic acid, 3-CQA), 3,5-dicaffeoylquinic acid (3,5-CQA), and 4,5-dicaffeoylquinic acid (4,5-CQA) were the major CADs in the hairy roots. Eight single roots with rapid growth rate were selected. Among them, T3 had the highest yield of CADs. B5 medium supplemented with 40 g/L sucrose was more suitable for the production of CADs than others. Under optimal culture conditions, the total content of these three compounds reached 105.58 mg/g and total yield was 234.40 mg/100 mL. |
abstract_unstemmed |
Chlorogenic acid and its derivatives (CADs) are valuable bioactive plant secondary metabolites with many health benefits. In the present study, Stevia rebaudiana hairy root cultures were established, and the culture conditions for the production of CADs were optimized. The hairy roots were induced by coculture of S. rebaudiana leaves and Agrobacterium rhizogenes (C58C1) after infection, which were further verified by PCR detection of rolB and rolC genes. HPLC-MS and HPLC analysis showed that chlorogenic acid (3-caffeoylquinic acid, 3-CQA), 3,5-dicaffeoylquinic acid (3,5-CQA), and 4,5-dicaffeoylquinic acid (4,5-CQA) were the major CADs in the hairy roots. Eight single roots with rapid growth rate were selected. Among them, T3 had the highest yield of CADs. B5 medium supplemented with 40 g/L sucrose was more suitable for the production of CADs than others. Under optimal culture conditions, the total content of these three compounds reached 105.58 mg/g and total yield was 234.40 mg/100 mL. |
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Production of chlorogenic acid and its derivatives in hairy root cultures of Stevia rebaudiana |
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Yin, Zhong-Ping Chen, Ji-Guang Shangguan, Xin-Chen Wang, Xiaoqiang Zhang, Qing-Feng Peng, Da-Yong |
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7.39999 |